scholarly journals DNA Barcode for the Parthenogenetic Surinam Cockroach Pycnoscelus surinamensis (Linnaeus, 1758) (Blattodea: Blaberidae) from India

2022 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
A Shabnam ◽  
K P Dinesh

DNA Barcoding is one of the emerging tools in molecular identification of faunal diversity, specifically insect fauna. The Surinam cockroach, Pycnoscelus surinamensis is the only known roach to be obligatorily parthenogenetic, with reported haplotypes. P. surinamensis is well established in Indomalayan, tropical and subtropical regions and substantially documented from India with a phenetic approach. Herewith we report the first set of mt DNA barcode from a vouchered collection for the species from southern Western Ghats India. Discussions are made on the identity of two sequences each of Blatteria species and Pycnoscelus species reported from USA.

Author(s):  
Qian Tang ◽  
Qi Luo ◽  
Qian Duan ◽  
Lei Deng ◽  
Renyi Zhang

Nowadays, the global fish consumption continues to rise along with the continuous growth of the population, which has led to the dilemma of overfishing of fishery resources. Especially high-value fish that are overfished are often replaced by other fish. Therefore, the accurate identification of fish products in the market is a problem worthy of attention. In this study, full-DNA barcoding (FDB) and mini-DNA barcoding (MDB) used to detect the fraud of fish products in Guiyang, Guizhou province in China. The molecular identification results showed that 39 of the 191 samples were not consistent with the labels. The mislabelling of fish products for fresh, frozen, cooked and canned were 11.70%, 20.00%, 34.09% and 50.00%, respectively. The average kimura 2 parameter distances of MDB within species and genera were 0.27% and 5.41%, respectively; while average distances of FDB were 0.17% within species and 6.17% within genera. In this study, commercial fraud is noticeable, most of the high-priced fish were replaced of low-priced fish with a similar feature. Our study indicated that DNA barcoding is a valid tool for the identification of fish products and that it allows an idea of conservation and monitoring efforts, while confirming the MDB as a reliable tool for fish products.


Author(s):  
Donald Hobern ◽  
Paul Hebert

Between 2010 and 2015, the International Barcode of Life (iBOL) consortium successfully completed the BARCODE 500K project, a $125 million effort that delivered DNA barcode coverage for 500,000 species. BIOSCAN is a seven-year program (2019-2025) that builds on this foundation, expanding coverage of the barcode reference library to two million species and operationalising metabarcoding for eukaryote communities globally. BIOSCAN will scan species assemblages from at least 2,500 ecosystems and will codify species interactions for at least 2,500 sites. DNA barcoding is a well-established approach for rapid, cost-effective species diagnosis, with many applications in support of taxonomy, biosecurity, conservation, and monitoring. Uptake has been particularly significant in hyperdiverse invertebrate groups where morphological approaches to species identification are often limiting (because of the scale of diversity and the small number of expert taxonomists) or inapplicable (for example in associating individuals from different life stages). The barcode reference library maintained as BOLD Systems by the Centre for Biodiversity Genomics in Guelph, Ontario is a significant biodiversity informatics infrastructure for bridging genomics and classical taxonomy, collections research, and field surveys. Effort across multiple years in Canada has delivered a library of reference sequences for the COI mitochondrial barcode that covers most of the known insect fauna for the country, enabling a comprehensive assessment of Canadian arthropod diversity (Hebert et al. 2016, Langor and Sheffield 2019). The Global Malaise Trap Program is expanding lessons learned in Canada to support species inventories in new regions such as Kruger National Park in South Africa. As DNA barcode libraries approach completeness for any site, analysis can employ metabarcoding to lower costs significantly for monitoring programs that track changes in species composition. Data from this program, and from barcode-based exploration in other regions, will greatly expand the fraction of biodiversity that can be monitored and compared over time and space. GBIF has collected more than one billion species records, but around 60% of these are for birds, with another 25% for vascular plants. Metabarcoding offers the opportunity for a wider selection of taxa to be included in global data sets and in support of local conservation and planning. The BIOSCAN program, launched by iBOL in 2019, seeks to operationalise DNA barcoding at the global scale for development of species inventories and preliminary exploration of undescribed diversity, for surveying community composition across the world's ecosystems, and codifying species interactions (the symbiome). BIOSCAN will exploit the latest advances in sequencing platforms to lower costs, increase precision, and accelerate processing of samples, to speed the uptake of DNA barcoding for protecting life on Earth.


2018 ◽  
Vol 19 (2) ◽  
pp. 364-368
Author(s):  
LULUT DWI SULISTYANINGSIH ◽  
MARLINA ARDIYANI ◽  
ABINAWANTO ABINAWANTO ◽  
ANDI SALAMAH

Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs especially in East Asia and North America as they were known to be rich in steroidal saponin. Pharmacological study has been carried out in Indonesia. This genus is widespread in Indonesia and fairly abundant in Java and has been known either as edible fruit or medicinal plants. Characteristics of Smilax as a dioecious plant with high morphological variations make it thorny in species identification. Various molecular approaches have been devised to overcome identification problems such as DNA barcoding. This study, therefore was conducted to analyze the DNA barcoding application for phylogenetic and identification of Smilax in Java. A total of 31 samples were used in this study including 19 accession numbers from NCBI GeneBank. The genus Ripogonum was used as the out-group in phylogenetic reconstruction. Samples were successfully extracted by CTAB method with some modifications. rbcL region was used as the DNA barcode showed sufficient variation and conserved flanks. Two unidentified specimens have high similarity with S. leucophyla and lies in the same clade. The phylogenetic tree constructed by Maximum Likelihood analysis. The result showed that the monophyletic of Smilacaceae consisted of four clades. The genus Heterosmilax nested with Smilax though with low bootstraps value. It supports the monogeneric status of Smilacaceae.


Acta Tropica ◽  
2015 ◽  
Vol 149 ◽  
pp. 94-105 ◽  
Author(s):  
S. Anbalagan ◽  
V. Arunprasanna ◽  
M. Kannan ◽  
S. Dinakaran ◽  
M. Krishnan

2018 ◽  
Vol 111 (1) ◽  
pp. 229
Author(s):  
Ajaz RASOOL ◽  
Tariq AHMAD ◽  
Bashir Ahmad GANAI ◽  
Shaziya GULL

Identifying organisms has grown in importance as we monitor the biological effects of global climate change and attempt to preserve species diversity in the face of accelerating habitat destruction. Classical taxonomy falls short in this race to catalogue biological diversity before it disappears. Differentiating subtle anatomical differences between closely related species requires the subjective judgment of highly trained specialists – and few are being trained in institutes today. DNA barcodes allow non-experts to objectively identify species – from small, damaged, or even industrially processed material. The aim of DNA barcoding is to establish a shared community resource of DNA sequences commonly used for identification, discrimination or taxonomic classification of organisms. It is a method that uses a short genetic marker in an organism's DNA to identify and distinguish its belonging from particular species, varieties or inter varieties. This simple technique has attracted attention from taxonomists, ecologists, conservation biologists, agriculturists, plant-quarantine officers and studies using the DNA barcode has rapidly increased. The extreme diversity of insects and their economical, epidemiological and agricultural importance have made them a major target of DNA barcoding. In this review, we present an overview of DNA barcoding of insects with emphasis on Chalcid wasps of India.


2020 ◽  
Vol 12 (9) ◽  
pp. 16143-16152
Author(s):  
Aparna Sureshchandra Kalawate ◽  
Shital Pawara ◽  
A. Shabnam ◽  
K.P. Dinesh

The present study was taken up to report a new record of the tiger moth genus, Olepa Watson, 1980 from India along with the discovery of a new subspecies.  Earlier  the genus was thought to have restricted distribution range in South and South-East Asia until the report of O. schleini Witt, Müller, Kravchenko, Miller, Hausmann & Speidel from the Mediterranean Coastal Plain of Israel in 2005. The species identification and the new subspecies is proposed based on the combination of morphological studies, available literature comparisons, geographical distribution,  DNA barcoding and its phylogeny. Morphological character crypticity and genital structure variations are well documented in the genus with ‘bio-species’ groups. DNA Barcoding data of mt COI has provided some resolution in sorting the problems of ‘bio-species’ groups of the genus in the past studies. In the present study, with the available  mt DNA COI barcodes and newly generated barcodes genetic identity is confirmed for the species O. ricini, O. schleini, O. toulgoeti and Olepa schleini chandrai ssp. nov., with their phylogenetic relationships. Morphological variations within the O. schleini species complex are discussed with a new record of the species for India and a new subspecies description. With the first mt COI barcode phylogeny for the genus, comments are made on the taxonomic identity of the mt COI DNA barcodes available in the GenBank for the Olepa species from India.  


ENTOMON ◽  
2019 ◽  
Vol 44 (1) ◽  
pp. 23-32 ◽  
Author(s):  
P. C. Sujitha ◽  
G. Prasad ◽  
R. Nitin ◽  
Dipendra Nath Basu ◽  
Krushnamegh Kunte ◽  
...  

Eurema nilgiriensis Yata, 1990, the Nilgiri grass yellow, was described from Nilgiris in southern India. There are not many published records of this species since its original description, and it was presumed to be a high-elevation endemic species restricted to its type locality. Based on the external morphology (wing patterns) as well as the male genitalia, the first confirmed records of the species from Agasthyamalais and Kodagu in the southern Western Ghats, is provided here. This report is a significant range extension for the species outside the Nilgiris, its type locality. Ecological data pertaining to this species as well as the field identification key to all known Eurema of Western Ghats are also presented.


2016 ◽  
Vol 2 (1) ◽  
pp. 46 ◽  
Author(s):  
Kalathummarath Shinoj ◽  
Kollancheri Puthanveetil Vimal ◽  
Purayidathkandy Sunojkumar

2015 ◽  
Vol 1 (1) ◽  
pp. 7 ◽  
Author(s):  
Chandransekar Balachandran ◽  
Veeramuthu Duraipandiyan ◽  
Nobuhiko Emi ◽  
Savarimuthu Ignacimuthu

2020 ◽  
Vol 12 (11) ◽  
pp. 16502-16509
Author(s):  
Sandeep Das ◽  
K.P. Rajkumar ◽  
K.A. Sreejith ◽  
M. Royaltata ◽  
P.S. Easa

Abstract: The Resplendent Shrub Frog, Raorchestes resplendens Biju, Shouche, Dubois, Dutta, & Bossuyt, 2010 is a Critically Endangered species endemic to the Western Ghats and was considered to be restricted to a three-square kilometer patch atop Anamudi summit.  In this study, we report 36 new locations of the species from the Anamalai massif of the southern Western Ghats.  Niche-based prediction modelling suggests that the species is restricted to Anamalai massif.  The call description of this frog is also provided for the first time. The preferred microhabitat of the frog is Chrysopogon grass clumps in the marshy/swampy montane grassland ecosystem. Restricted to a small area with controlled burning management practiced in its habitat, R. resplendens needs immediate attention.


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