Isolation and characterization of bacteriophages from wastewater sources on Enterococcus spp. isolated from clinical samples

Iranian Journal of Microbiology ◽

10.18502/ijm.v13i5.7434 ◽

2021 ◽

Author(s):

Yara Elahi ◽

Jamileh Nowroozi ◽

Ramin Mazaheri Nezhad Fard

Keyword(s):

Multidrug Resistant ◽

Eukaryotic Cells ◽

Clinical Samples ◽

Streptococcal Infections ◽

Bacterial Isolates ◽

Streptococcus Dysgalactiae ◽

Antibiotic Resistant ◽

Isolation And Characterization ◽

Enterococcus Spp

Background and Objectives: In recent decades, enterococcal resistance to antimicrobials has greatly increased. Further- more, these chemicals include several side effects on the patients. Since no reports are available of the bacteriophages' effects on eukaryotic cells, they can be good solutions for multidrug-resistant bacterial problems. Therefore, the major aim of this study was to isolate bacteriophages from wastewaters on clinical antibiotic-resistant enterococci. Materials and Methods: Clinical bacteria were isolated, then enterococcal isolates were identified using different methods. The antibiotic resistance scheme of the enterococcal isolates was assessed. The bacterial isolates were exposed to wastewa- ter samples containing potential bacteriophages. Technically, isolated bacteriophages were studied by electron microscopy. Results: Isolated bacteria were verified as Enterococcus faecium. Results showed that bacteriophages could easily be isolat- ed from wastewater sources. The isolated bacteriophages were effective on E. faecium as well as Streptococcus dysgalactiae. Furthermore, these bacteriophages were challenged with five other bacteria (ATCC) with no visible effects. In general, the isolated bacteriophages belonged to the Myoviridae, Siphoviridae, and Inoviridae families. Conclusion: Further studies on bacteriophages and their efficacy on enterococcal strains could increase the treatment possi- bility of enterococcal infections. Due to these bacteriophages' effects on Streptococcus strains, bacteriophages may be used to treat streptococcal infections as well.

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Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods

Microorganisms ◽

10.3390/microorganisms9020423 ◽

2021 ◽

Vol 9 (2) ◽

pp. 423

Author(s):

Ahmed Esmael ◽

Ehab Azab ◽

Adil A. Gobouri ◽

Mohamed A. Nasr-Eldin ◽

Mahmoud M. A. Moustafa ◽

...

Keyword(s):

Salmonella Typhimurium ◽

Multidrug Resistant ◽

Viable Count ◽

Food Storage ◽

Chicken Breast ◽

Whole Genome Analysis ◽

Isolation And Characterization ◽

Phage Cocktail ◽

Global Threat

Foodborne salmonellosis is a global threat to public health. In the current study, we describe the isolation and characterization of two broad-spectrum, lytic Salmonella phages: SPHG1 and SPHG3 infecting a multidrug-resistant Salmonella Typhimurium EG.SmT3. Electron microscopy and whole genome analysis identified SPHG1 as a Myovirus, while SPHG3 as a new member of the genus “Kuttervirus” within the family Ackermannviridae. SPHG1 and SPHG3 had a lysis time of 60 min. with burst sizes of 104 and 138 PFU/cell, respectively. The two phages were robust at variable temperatures and pH ranges that match the corresponding values of most of the food storage and processing conditions. A phage cocktail containing the two phages was stable in the tested food articles for up to 48 h. The application of the phage cocktail at MOIs of 1000 or 100 resulted in a significant reduction in the viable count of S. Typhimurium by 4.2 log10/sample in milk, water, and on chicken breast. Additionally, the phage cocktail showed a prospective ability to eradicate and reduce the biofilm that formed by S. Typhimurium EG.SmT3. A phage cocktail of SPHG1 and SPHG3 is considered as a promising candidate as a biocontrol agent against foodborne salmonellosis due to its broad host ranges, highly lytic activities, and the absence of any virulence or lysogeny-related genes in their genomes.

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Purification and characterization of bacteriocins-like inhibitory substances from food isolated Enterococcus faecalis OS13 with activity against nosocomial enterococci

Scientific Reports ◽

10.1038/s41598-021-83357-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ahmed O. El-Gendy ◽

Dag A. Brede ◽

Tamer M. Essam ◽

Magdy A. Amin ◽

Shaban H. Ahmed ◽

...

Keyword(s):

Enterococcus Faecalis ◽

Antimicrobial Agents ◽

Food Samples ◽

Proteinase K ◽

Clinical Samples ◽

Bile Salt Hydrolase ◽

Antibiotic Resistant ◽

Molecular Weights ◽

Global Threat

AbstractNosocomial infections caused by enterococci are an ongoing global threat. Thus, finding therapeutic agents for the treatment of such infections are crucial. Some Enterococcus faecalis strains are able to produce antimicrobial peptides called bacteriocins. We analyzed 65 E. faecalis isolates from 43 food samples and 22 clinical samples in Egypt for 17 common bacteriocin-encoding genes of Enterococcus spp. These genes were absent in 11 isolates that showed antimicrobial activity putatively due to bacteriocins (three from food, including isolate OS13, and eight from clinical isolates). The food-isolated E. faecalis OS13 produced bacteriocin-like inhibitory substances (BLIS) named enterocin OS13, which comprised two peptides (enterocin OS13α OS13β) that inhibited the growth of antibiotic-resistant nosocomial E. faecalis and E. faecium isolates. The molecular weights of enterocin OS13α and OS13β were determined as 8079 Da and 7859 Da, respectively, and both were heat-labile. Enterocin OS13α was sensitive to proteinase K, while enterocin OS13β was resistant. Characterization of E. faecalis OS13 isolate revealed that it belonged to sequence type 116. It was non-hemolytic, bile salt hydrolase-negative, gelatinase-positive, and sensitive to ampicillin, penicillin, vancomycin, erythromycin, kanamycin, and gentamicin. In conclusion, BLIS as enterocin OS13α and OS13β represent antimicrobial agents with activities against antibiotic-resistant enterococcal isolates.

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Isolation and Characterization of Novel Human Parechovirus from Clinical Samples

Emerging Infectious Diseases ◽

10.3201/eid1306.060896 ◽

2007 ◽

Vol 13 (6) ◽

pp. 889-895 ◽

Cited By ~ 105

Author(s):

Kanako Watanabe ◽

Masayasu Oie ◽

Masaya Higuchi ◽

Makoto Nishikawa ◽

Masahiro Fujii

Keyword(s):

Clinical Samples ◽

Human Parechovirus ◽

Isolation And Characterization

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Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

Aceh International Journal of Science and Technology ◽

10.13170/aijst.4.3.3016 ◽

2015 ◽

Vol 4 (3) ◽

Author(s):

Kesi Kurnia ◽

Nina Hermayani Sadi ◽

Syafitri Jumianto

Keyword(s):

Heterotrophic Bacteria ◽

Water Environment ◽

Resistant Bacteria ◽

Bacterial Isolates ◽

Gram Negative ◽

Isolation And Characterization ◽

Agar Media ◽

Physical And Chemical ◽

Standard Disk

Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. This study aim to isolate and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, West Java, Indonesia. The water samples were collected along three points around Cilalay Lake. Water physical and chemical determination was performed using the Water Quality Checker. The bacterial isolates were screened on Triptone Glucose Yeast (TGY) agar plates. Afterwards selected isolates were grown on Nutrient Agar media 50% with supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates were resistant. The most abundant type of resistant bacteria to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water

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Isolation and Characterization of Two Klebsiella pneumoniae Phages Encoding Divergent Depolymerases

International Journal of Molecular Sciences ◽

10.3390/ijms21093160 ◽

2020 ◽

Vol 21 (9) ◽

pp. 3160 ◽

Cited By ~ 1

Author(s):

Pilar Domingo-Calap ◽

Beatriz Beamud ◽

Lucas Mora-Quilis ◽

Fernando González-Candelas ◽

Rafael Sanjuán

Keyword(s):

Klebsiella Pneumoniae ◽

Multidrug Resistant ◽

Health Concern ◽

Resistant Bacteria ◽

Isolation And Characterization ◽

The Family ◽

Important Challenge ◽

Tail Spike ◽

Reference Strains

The emergence of multidrug-resistant bacteria is a major global health concern. The search for new therapies has brought bacteriophages into the spotlight, and new phages are being described as possible therapeutic agents. Among the bacteria that are most extensively resistant to current antibiotics is Klebsiella pneumoniae, whose hypervariable extracellular capsule makes treatment particularly difficult. Here, we describe two new K. pneumoniae phages, πVLC5 and πVLC6, isolated from environmental samples. These phages belong to the genus Drulisvirus within the family Podoviridae. Both phages encode a similar tail spike protein with putative depolymerase activity, which is shared among other related phages and probably determines their ability to specifically infect K. pneumoniae capsular types K22 and K37. In addition, we found that phage πVLC6 also infects capsular type K13 and is capable of striping the capsules of K. pneumoniae KL2 and KL3, although the phage was not infectious in these two strains. Genome sequence analysis suggested that the extended tropism of phage πVLC6 is conferred by a second, divergent depolymerase. Phage πVLC5 encodes yet another putative depolymerase, but we found no activity of this phage against capsular types other than K22 and K37, after testing a panel of 77 reference strains. Overall, our results confirm that most phages productively infected one or few Klebsiella capsular types. This constitutes an important challenge for clinical applications.

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Isolation and characterization of bacteriophage to control multidrug-resistant Pseudomonas aeruginosa planktonic cells and biofilm

Biologicals ◽

10.1016/j.biologicals.2019.10.003 ◽

2020 ◽

Vol 63 ◽

pp. 89-96 ◽

Cited By ~ 3

Author(s):

Muhammad Adnan ◽

Muhammad Rahman Ali Shah ◽

Muhsin Jamal ◽

Fazal Jalil ◽

Saadia Andleeb ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Multidrug Resistant ◽

Planktonic Cells ◽

Isolation And Characterization

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Technological Feature Assessment of Lactic Acid Bacteria Isolated from Cricket Powder’s Spontaneous Fermentation as Potential Starters for Cricket-Wheat Bread Production

Foods ◽

10.3390/foods9091322 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1322

Author(s):

Viola Galli ◽

Manuel Venturi ◽

Niccolò Pini ◽

Lisa Granchi

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Wheat Bread ◽

Aminopeptidase Activity ◽

Spontaneous Fermentation ◽

Baked Goods ◽

Isolation And Characterization ◽

Enterococcus Spp ◽

Bread Production

The bacterial community profile of cricket powder highlighted the presence of four main genera: Bacteroides spp., Parabacteroides spp., Lactococcus spp., and Enterococcus spp. The spontaneous fermentation of cricket powder allowed for the isolation and characterization of seven lactic acid bacteria strains belonging to six species: Latilactobacillus curvatus, Lactiplantibacillus plantarum, Latilactobacillus sakei, Lactococcus garvieae, Weissella confusa, and Enterococcus durans. The strains were characterized and selected according to different technological properties. L. plantarum CR L1 and L. curvatus CR L13 showed the best performance in terms of general aminopeptidase activity, acidification, and growth rate in MRS broth and in dough with cricket powder and wheat flour, as well as robustness during consecutive backslopping. Thus, they were used as starter-mixed to produce sourdough to manufacture bread fortified with 20% cricket powder. The addition of cricket powder led to a significant increase of protein (up to 94%) and lipid content, from 0.7 up to 6 g/100 g of bread. Spontaneous fermentation represents a source of microbial diversity that can be exploited in order to obtain potential starters for food with innovative ingredients. Edible insects powder can be successfully added in leavened baked goods to enhance their nutritional value.

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Isolation and characterization of multidrug-resistant Leclercia species from animal clinical case

Letters in Applied Microbiology ◽

10.1111/lam.12811 ◽

2017 ◽

Vol 66 (1) ◽

pp. 44-48 ◽

Cited By ~ 4

Author(s):

M. Choudhary ◽

B.K. Choudhary ◽

S. Bhoyar ◽

S.B. Kale ◽

S.P. Chaudhari ◽

...

Keyword(s):

Clinical Case ◽

Multidrug Resistant ◽

Isolation And Characterization

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Isolation and characterization of bacteria from the rhizosphere and bulk soil of Stellera chamaejasme L.

Canadian Journal of Microbiology ◽

10.1139/cjm-2014-0543 ◽

2015 ◽

Vol 61 (3) ◽

pp. 171-181 ◽

Cited By ~ 7

Author(s):

Haiyan Cui ◽

Xiaoyan Yang ◽

Dengxue Lu ◽

Hui Jin ◽

Zhiqiang Yan ◽

...

Keyword(s):

Bulk Soil ◽

Growth Stages ◽

Bacterial Isolates ◽

Phenotypic Properties ◽

Isolation And Characterization ◽

Fruiting Stage ◽

Major Shift ◽

Stellera Chamaejasme ◽

Bacterial Groups

This study is the first to describe the composition and characteristics of culturable bacterial isolates from the rhizosphere and bulk soil of the medicinal plant Stellera chamaejasme L. at different growth stages. Using a cultivation-dependent approach, a total of 148 isolates showing different phenotypic properties were obtained from the rhizosphere and bulk soil. Firmicutes and Actinobacteria were the major bacterial groups in both the rhizosphere and bulk soil at all 4 growth stages of S. chamaejasme. The diversity of the bacterial community in the rhizosphere was higher than that in bulk soil in flowering and fruiting stages. The abundance of bacterial communities in the rhizosphere changed with the growth stages and had a major shift at the fruiting stage. Dynamic changes of bacterial abundance and many bacterial groups in the rhizosphere were similar to those in bulk soil. Furthermore, most bacterial isolates exhibited single or multiple biochemical activities associated with S. chamaejasme growth, which revealed that bacteria with multiple physiological functions were abundant and widespread in the rhizosphere and bulk soil. These results are essential (i) for understanding the ecological roles of bacteria in the rhizosphere and bulk soil and (ii) as a foundation for further evaluating their efficacy as effective S. chamaejasme growth-promoting rhizobacteria.

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