scholarly journals The Development of a Technique for Sensitizing Cells of Various Origins for Biotechnological Purposes

2022 ◽  
Author(s):  
Anna Oleshkevich ◽  
Elena Yarygina
Keyword(s):  
Cell Culture ◽  
Traveling Wave ◽  
Proliferative Activity ◽  
Modulation Frequency ◽  
Mdbk Cell ◽  
Proliferation Index ◽  
Frequency Range ◽  
Generation Frequency ◽  
Modulated Waves ◽  
Stimulation Of

The functional activity stimulation of cell cultures was tested in MDBK cell culture, photobacteria AliivibriofischeriandHalobacteriumhalobium. Theaim of the investigation was to increase the ”yield” of the cells using an environmentallysafe stimulant and membrane-tropic agent that isalso safe for the experimenter. Ultrasonicwaves were used.Experimental ultrasonic exposure varied within the following limits: time from 1 to 300 sec, SATA-intensity of 0.01–2.0 W/cm2, generation frequency of 0.88 or 2.64 MHz, standing or traveling wave. The modulation frequency range was within 0.1–150 Hz. The devices used were: UST-1-01F, UST-5 and UST1.02C. The modulating generators were G3–112 and CP–110.Stimulation of MDBK cell growth was initiated by US-intensity of 0.03–0.05 W/cm2 , with an exposure of 5–30 sec.Exposure to ultrasound with an intensity of 0.2–0.4 W/cm2 (for 3 min) had a stimulating effect on bioluminescence and was associated with an increase in the growth rate ofA. fischeri. The findings indicated that 0.4 W/cm2ultrasonic intensity and modulation frequencies from 0.25 to 0.7 Hz can stimulate the growth of archaea.It was revealed that the maximum proliferation index in all cases of stimulant application was noted in cultures with minimal initial proliferative activity in the control.The authors expect thatthese results on the possibilities of acoustic continuous and modulated waves can be applied for biotechnological purposes to develop a new biotechnological method. Keywords: cell culture, ultrasound, proliferation, stimulation

scholarly journals Interleukin-8 and Growth-Regulated Oncogene Alpha Mediate Angiogenesis in Kaposi's Sarcoma

2002 ◽  
Vol 76 (22) ◽  
pp. 11570-11583 ◽  
Author(s):  
Brian R. Lane ◽  
Jianguo Liu ◽  
Paul J. Bock ◽  
Dominique Schols ◽  
Michael J. Coffey ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Interleukin 8 ◽  
Cellular Growth ◽  
Immunodeficiency Virus ◽  
Hiv 1 ◽  
Stimulation Of

ABSTRACT The development of the complex neoplasm Kaposi's sarcoma is dependent on infection with the Kaposi's sarcoma-associated herpesvirus (KSHV) and appears to be greatly enhanced by cytokines and human immunodeficiency virus type 1 (HIV-1) Tat. Interleukin-8 (IL-8) and growth-regulated oncogene alpha (GRO-α) are chemokines involved in chemoattraction, neovascularization, and stimulation of HIV-1 replication. We have previously demonstrated that production of GRO-α is stimulated by exposure of monocyte-derived macrophages (MDM) to HIV-1. Here we show that exposure of MDM to HIV-1, viral Tat, or viral gp120 leads to a substantial increase in IL-8 production. We also demonstrate that IL-8 and GRO-α are induced by KSHV infection of endothelial cells and are crucial to the angiogenic phenotype developed by KSHV-infected endothelial cells in cell culture and upon implantation into SCID mice. Thus, the three known etiological factors in Kaposi's sarcoma pathogenesis—KSHV, HIV-1 Tat, and cellular growth factors—might be linked, in part, through induction of IL-8 and GRO-α.

scholarly journals Regulation of angiopoietin-like protein 4/fasting-induced adipose factor (Angptl4/FIAF) expression in mouse white adipose tissue and 3T3-L1 adipocytes

2008 ◽  
Vol 100 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Sarah Dutton ◽  
Paul Trayhurn
Keyword(s):  
Skeletal Muscle ◽  
Cell Culture ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Culture Medium ◽  
Mrna Level ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Before And After ◽  
Stimulation Of

Angiopoietin-like protein 4 (Angptl4)/FIAF (fasting-induced adipose factor) was first identified as a target for PPAR and to be strongly induced in white adipose tissue (WAT) by fasting. Here we have examined the regulation of the expression and release of this adipokine in mouse WAT and in 3T3-L1 adipocytes. Angptl4/FIAF expression was measured by RT-PCR and real-time PCR; plasma Angptl4/FIAF and release of the protein in cell culture was determined by western blotting. The Angptl4/FIAF gene was expressed in each of the major WAT depots of mice, the mRNA level in WAT being similar to the liver and much higher (>50-fold) than skeletal muscle. Fasting mice (18 h) resulted in a substantial increase in Angptl4/FIAF mRNA in liver and muscle (9·5- and 21-fold, respectively); however, there was no effect of fasting on Angptl4/FIAF mRNA in WAT and the plasma level of Angptl4/FIAF was unchanged. The Angptl4/FIAF gene was expressed in 3T3-L1 adipocytes before and after differentiation, the level increasing post-differentiation; Angptl4/FIAF was released into the culture medium. Insulin, leptin, dexamethasone, noradrenaline, TNFα and several IL (IL-1β, IL-6, IL-10, IL-18) had little effect on Angptl4/FIAF mRNA levels in 3T3-L1 adipocytes. However, a major stimulation of Angptl4/FIAF expression was observed with rosiglitazone and the inflammatory prostaglandins PGD2 and PGJ2. Angptl4/FIAF does not act as an adipose tissue signal of nutritional status, but is markedly induced by fasting in liver and skeletal muscle.

scholarly journals Photodetector resistant to background light noise with extended dynamic range of input signals

2021 ◽  
pp. 3-8
Author(s):  
V. M. Lipka ◽  
V. V. Ryukhtin ◽  
Yu. G. Dobrovolsky
Keyword(s):  
Dynamic Range ◽  
Automatic Gain Control ◽  
Modulation Frequency ◽  
Low Frequency ◽  
Gain Control ◽  
Ambient Air ◽  
Frequency Range ◽  
Background Light ◽  
Useful Signal ◽  
Extended Dynamic

Measurement of periodic optical information signals in the background light noise with a photodetector with extended dynamic range is an urgent task of modern electronics and thus has become the aim of this study. To increase the dynamic range of the photodetector, a new version of the automatic gain control (AGC) circuit has been developed, which consists of an AGC controller, an output photodetector amplifier and an AGC detector. The authors measured the dynamic range of the photodetector when receiving optical radiation with a wavelength of 1064 nm in the power range from 2.10–8 to 2.10–5 W at a modulation frequency of 20 kHz with the AGC on. Under these conditions, the dynamic range of the photodetector was found to be up to 67 dB. If the AGC was off, the dynamic range did not exceed 30 dB. Thus, the study made it possible to create a photodetector with an extended dynamic range up to 67 dB based on a new version of the AGC circuit. The design of the photodetector allowed choosing a useful signal of a particular modulation frequency in the frequency range from 3 to 45 kHz and effectively suppresses the frequencies caused by optical interference in the low frequency range from the frequency of the input signal of constant amplitude up to 3 kHz inclusive. This compensates the current up to 15 mA, which is equivalent to the power of light interference of about 15 mW. Further research should address the issues of reliability of the proposed photodetector design and optimization of its optical system. The photodetector can be used in geodesy and ambient air quality monitoring.

scholarly journals Localized mechanical stimulation of single cells with engineered spatio-temporal profile

Lab on a Chip ◽  
2018 ◽  
Vol 18 (19) ◽  
pp. 2955-2965 ◽  
Author(s):  
M. Monticelli ◽  
D. S. Jokhun ◽  
D. Petti ◽  
G. V. Shivashankar ◽  
R. Bertacco
Keyword(s):  
Cell Culture ◽  
Mechanical Stimulation ◽  
Single Cells ◽  
Mechanical Stimuli ◽  
Temporal Profile ◽  
A Cell ◽  
Spatio Temporal ◽  
Stimulation Of

We introduce a new platform for mechanobiology based on active substrates, made of Fe-coated polymeric micropillars, capable to apply mechanical stimuli with tunable spatio-temporal profile on a cell culture.

Expansion and Contraction of the Oral Disc in the Sea Anemone Tealia Felina

1972 ◽  
Vol 57 (3) ◽  
pp. 633-649
Author(s):  
I. D. MCFARLANE ◽  
I. D. LAWN
Keyword(s):  
Recovery Period ◽  
Stimulus Frequency ◽  
Sensory Response ◽  
Frequency Range ◽  
Oral Disc ◽  
Nerve Net ◽  
Slow Contraction ◽  
Slow Onset ◽  
Radial Muscle ◽  
Stimulation Of

1. Electrical stimulation of the SS 1 of Tealia felina causes inhibition of spontaneousactivity and increase in length of oral disc radial muscle preparations. This response is elicited over a wide stimulus frequency range (1 every 2 sec to 1 every 30 sec). The response shows a slow onset and a long recovery period. 2. Stimulation of the nerve net at frequencies between 1 shock every 5 sec and 1 shock every 20 sec produces slow contraction. The radials also show fast contractions to shocks less than 2 sec apart. 3. Dissolved food substances excite the SS 1 in the column. The sensory response to application of food extract to a small area of the column shows evidence of sensory adaptation. 4. These observations are related to the pre-feeding response of Tealia and a model for oral disc expansion is described.

An optimized model for hCG stimulation of human mural granulosa cell culture

2019 ◽  
Vol 19 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Libby Ophir ◽  
Yuval Yung ◽  
Gil Mordechai Yerushalmi ◽  
Micha Baum ◽  
Ronit Machtinger ◽  
...  
Keyword(s):  
Cell Culture ◽  
Granulosa Cell ◽  
Optimized Model ◽  
Stimulation Of
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