scholarly journals The importance of bovine seminal plasma proteins in the sperm function and fertility

SPERMOVA ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 83-95
Author(s):  
María Alejandra Cardozo ◽  
◽  
Jaime Antonio Cardozo ◽  
Fabian Rueda

Bovine livestock is one of the most important economic and social sectors for many countries. In this sense, the development of strategies to improve reproductive bull fertility and reproduction rates is relevant. It's highlighted the role of seminal plasma proteins (SPP) in reproductive fertility, so it has found close relationships among studies on the structure and biological activity of SPP, with seminal quality, including viability, sperm motility, and morphology. In addition, they have been found to regulate sperm functions such as capacitation, acrosome reaction, and they are even related to protecting sperm against thermal and oxidative stress. Moreover, the methods of separation and protein identification and their contribution to characterizing the bovine SP proteome should be also highlighted. In this sense, the most recent studies have been directed towards developing supplements with SPP that improve quality sperm subjected to cryopreservation processes. Research has begun and should forward to establish how the networks or sets of proteins are related to the functioning and fertility of sperm, the search for biomarkers of fertility, and the use of proteins in biotechnological processes, to increase efficiency reproductive.

2018 ◽  
Vol 30 (1) ◽  
pp. 165 ◽  
Author(s):  
J. Rungruangsak ◽  
J. Suwimonteerabutr ◽  
S. Asawakarn ◽  
K. Buranaamnuay ◽  
N. Chantaravisoot ◽  
...  

Seminal plasma is the semen components that maintain sperm metabolism, pH and osmolality. Fibronectin (FN1) and glutathione peroxidase (GPX5) are the seminal plasma proteins that play an important role on the boar sperm functions. The purpose of the present study was to determine the differences in GPX5 and FN1 contents in the boar semen having good, moderate, and poor freezability. A total of 38 ejaculates from 25 boars with proven fertility were included in the experiment. All the ejaculates included in the study had >70% subjective motility, >75% normal morphology, >75% sperm viability, and volume >100 mL per ejaculate. The semen was collected through semen collection bag with filter and split into 2 portions. The first portion was prepared for the evaluation of seminal plasma proteins (i.e. GPX5 and FN1) and the second portion was cryopreserved and evaluated for post-thaw semen qualities. The seminal plasma samples were collected in cryotube and plug into liquid nitrogen. The samples was stored at –80°C before protein extraction. After thawing, the ejaculates were classified into 3 groups according to their post-thawed sperm motility: poor (14.6 ± 3.9%), modersate (28.5 ± 4.1%), and good (64.0 ± 8.7%) freezability. The amounts of GPX5 and FN1 proteins were evaluated through Western blot analysis. The normalized quantity of proteins was compared among groups by one-way ANOVA. The normalized level of FN1 in seminal plasma was higher in good- than in poor-freezability groups (8.0 ± 0.8% v. 5.7 ± 0.7%, respectively; P < 0.05), but did not differ significantly compared with that of the moderate-freezability group (7.5 ± 0.8%; P > 0.05). The levels of GPX5 in good-, moderate-, and poor-freezability groups were 14.7 ± 3.0%, 16.8 ± 3.2%, and 10.9 ± 3.0%, respectively (P > 0.05). The level of FN1 in seminal plasma was significantly correlated with the post-thaw sperm progressive motility (r = 0.38, P = 0.01), total motility (r = 0.37, P = 0.02), and the proportion of bent tail sperm (r = –0.33, P = 0.04). The level of GPX5 was not correlated with any of the post-thaw sperm qualities (P > 0.05). However, the levels of GPX5 was positively correlated with FN1 (r = 0.40, P = 0.01, n = 38). It can be concluded that FN1 in seminal plasma can be used as a marker of sperm freezability in boar.


2019 ◽  
Vol 26 (4) ◽  
pp. 238-250 ◽  
Author(s):  
Archana Kumar ◽  
T.B. Sridharn ◽  
Kamini A. Rao

Seminal plasma proteins contributed by secretions of accessory glands plays a copious role in fertilization. Their role is overlooked for decades and even now, as Artificial Reproduction Techniques (ART) excludes the plasma components in the procedures. Recent evidences suggest the importance of these proteins starting from imparting fertility status to men, fertilization and till successful implantation of the conceptus in the female uterus. Seminal plasma is rich in diverse proteins, but a major part of the seminal plasma is constituted by very lesser number of proteins. This makes isolation and further research on non abundant protein a tough task. With the advent of much advanced proteomic techniques and bio informatics tools, studying the protein component of seminal plasma has become easy and promising. This review is focused on the role of seminal plasma proteins on various walks of fertilization process and thus, the possible exploitation of seminal plasma proteins for understanding the etiology of male related infertility issues. In addition, a compilation of seminal plasma proteins and their functions has been done.


2010 ◽  
Vol 22 (6) ◽  
pp. 893 ◽  
Author(s):  
Melissa L. Vadnais ◽  
Kenneth P. Roberts

Dilute boar seminal plasma (SP) has been shown to inhibit in vitro capacitation and cooling-induced capacitation-like changes in boar spermatozoa, as assessed by the ability of the spermatozoa to undergo an ionophore-induced acrosome reaction. We hypothesised that the protein component of SP is responsible for this effect. To test this hypothesis, varying concentrations of total SP protein or SP proteins fractionated by heparin binding were assayed for their ability to inhibit in vitro capacitation, as well as cooling- and cryopreservation-induced capacitation-like changes. In vitro capacitation and cooling-induced capacitation-like changes were prevented by 10% whole SP, as well as by total proteins extracted from SP at concentrations greater than 500 μg mL−1. No amount of SP protein was able to prevent cryopreservation-induced capacitation-like changes. Total SP proteins were fractionated based on their heparin-binding properties and the heparin-binding fraction was shown to possess capacitation inhibitory activity at concentrations as low as 250 µg mL−1. The proteins in the heparin-binding fraction were subjected to mass spectrometry and identified. The predominant proteins were three members of the spermadhesin families, namely AQN-3, AQN-1 and AWN, and SP protein pB1. We conclude that one or more of these heparin-binding SP proteins is able to inhibit in vitro capacitation and cooling-induced capacitation-like changes, but not cryopreservation-induced capacitation-like changes, in boar spermatozoa.


2011 ◽  
Vol 14 (3) ◽  
pp. 489-499 ◽  
Author(s):  
M. Mogielnicka-Brzozowska ◽  
W. Kordan

Characteristics of selected seminal plasma proteins and their application in the improvement of the reproductive processes in mammals Understanding the biochemical processes associated with ovum fertilization and knowledge about the structure and function of individual substances participating in these processes is crucial for the development of biotechnological methods to improve reproduction of animals and humans. Among many components of seminal plasma, proteins and peptides play a specific role in regulation of the fertilization process, particularly through their ability to bind various types of ligands such as polysaccharides, lipids and ions. Heparin-binding proteins regulate capacitation and acrosome reaction processes. Affinity of plasma proteins to mannans of the fallopian tube epithelium facilitates formation of spermatozoa reservoirs in the female reproductive tract. Ability to bind phosphorylcholine is one of the conditions for the coating of the seminal plasma proteins on the sperm membrane and also determines the formation of oligomeric forms of certain proteins. Zinc binding by seminal plasma proteins regulates sperm chromatin condensation state. It also affects motility of these cells and acrosome reaction. The interspecies analysis indicates significant structural and functional similarities, especially for the proteins with low molecular weight. Fertility associated proteins (FAPs) have been determined in the bull, stallion, boar, ram and dog. The contents of these proteins correlate with the indicators of the fertilizing abilities of sperm. In humans, several seminal plasma proteins were found which serve as diagnostic markers of spermatogenesis, seminiferous epithelium state, and azoospermia. To determine the semen ability for preservation, measurement of some seminal plasma protein content may also be used. Addition of specific plasma proteins to a spermatozoa solution undergoing the process of preservation may be used to retain the features of the cells responsible for efficient fertilization.


2018 ◽  
Vol 195 ◽  
pp. 251-258 ◽  
Author(s):  
Viviane Maria Codognoto ◽  
Paulo Henrique Yamada ◽  
Rúbia Alves Schmith ◽  
Felipe Rydygier de Ruediger ◽  
Caroline Scott ◽  
...  

1993 ◽  
Vol 64 (3) ◽  
pp. 221-227
Author(s):  
Soichi TSUJI ◽  
Masatoshi ASAO ◽  
Hiroshi KUSUNOKI ◽  
Takao OISHI

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Paula Lorena Souto ◽  
Lilian Silveira Travassos Carmouy ◽  
Cristiane Santos ◽  
Edison Martins ◽  
Vera Martins ◽  
...  

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