scholarly journals Screening of Mango Landraces for Polyembryony and Confirmation of Seedling Origin using Microsatellite Markers

2021 ◽  
Author(s):  
U.R. Reshma ◽  
S. Simi
Keyword(s):  
Early Stage ◽  
Microsatellite Analysis ◽  
Mother Plant ◽  
Seedling Vigour ◽  
Banding Patterns ◽  
Genetic Characteristics ◽  
Randomized Design ◽  
Vigour Index ◽  
Mother Plants ◽  
Hybridization Programme

Background: The introduction of polyembryonic rootstocks in the area of propagation is of great importance since they produce one zygotic and several nucellar plantlets. Proper identification of sexual embryo from each hybrid seed is necessary in order to preserve only the nucellar seedlings, which would help to maintain the rootstock’s genetic characteristics as well as to overcome the major constraints in the area of fruit breeding especially in hybridization programme by eliminating the nucellar ones to advanced generations. Contrasting reports exists regarding the vigour of zygotic seedlings of polyembryonic mango genotypes. It is necessary to identify/ distinguish the zygotic seedling from the nucellar population at an early stage, for which, microsatellite analysis could be a reliable tool. Methods: The experiment was laid out in completely randomized design (CRD) with 20 treatments replicated thrice. The twenty local mango landraces from Thiruvananthapuram (Kerala) were screened for polyembryony and were geo-referenced. Germination studies were conducted. Microsatellite analysis of all the plantlets from two varieties which exhibited the highest polyembryony were done using SSR primers and their banding patterns were compared with those of their respective mother plants. Result: Out of twenty mango varieties screened, seventeen were polyembryonic. Kappa Manga recorded the highest germination, germination index and seedling vigour index-I. Kotookonam Varikka recorded the highest polyembryony and followed by Kochu Kilichundan. Microsatellite analysis revealed that all the seedlings obtained from the respective stones of Kotookonam Varikka and Kochu Kilichundan had identical SSR profile to the mother plant, which indicated nucellar origin of seedlings having similar genetic composition to the mother plant.

scholarly journals Results in the determination of some Hosta varieties by the method of isoelectric focusing

2001 ◽  
Vol 7 (1) ◽  
Author(s):  
A. Mándy ◽  
É. Stefanovits-Bányai ◽  
Cs. Imre ◽  
Zs. Szafián
Keyword(s):  
Isoelectric Focusing ◽  
Early Stage ◽  
Mother Plant ◽  
Isoenzyme Pattern ◽  
Easy Method ◽  
Mother Plants ◽  
Peroxidase Enzymes ◽  
Hybrid Lines ◽  
Isoenzyme Patterns

Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.  

scholarly journals Effect of Gibberellic Acid on Germination and Vigour of Kagzi Lime Seedlings

2019 ◽  
pp. 1-8
Author(s):  
Asha Chaudhary ◽  
T. R. Ahlawat ◽  
Sanjeev Kumar ◽  
Suchismita Jena ◽  
Dharmishtha Patel
Keyword(s):  
Aqueous Solution ◽  
Gibberellic Acid ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Survival Percentage ◽  
Randomized Design ◽  
Early Germination ◽  
Vigour Index ◽  
Number Of Leaves ◽  
Collar Diameter

Aims: To investigate the effect of storage treatments, gibberellic acid (GA3) and duration of soaking on seed germination and seedling growth in Kagzi lime. Study Design: The experiment was laid out in a Completely Randomized Design (CRD) with factorial concept and three repetitions. Place and Duration of Study: The experiment was conducted during July to November 2017 at Department of Fruit Science, Navsari Agricultural University, Navsari, Gujarat, India. Methodology: The experiment consisted of sixteen treatments which were repeated thrice. It comprised of three factors and their respective levels. Factor S1 indicated freshly extracted kagzi lime seeds and S2 comprised of seeds stored for 15 days. The different concentrations of gibberellic acid were G1 - 200 ppm; G2 - 300 ppm; G3 - 400 ppm and G4 - 500 ppm. Whereas, duration D1 stood for 12 hours and D2 for 24 hours. Results: The interaction between storage treatments, pre-sowing treatments and duration of soaking was found significant for all parameters included in the study except collar diameter and sturdiness quotient. Soaking freshly extracted Kagzi lime seeds in an aqueous solution of 500 ppm GA3 for 12 hours resulted in the minimum days for 50% germination (27.89), Seedling Vigour Index (3654.83) and survival percentage (84.70). The same treatment when extended for 24 hours recorded the highest germination percentage (95.68) and number of leaves (28.76). Soaking freshly extracted seeds in 400 ppm GA3 solution for 12 hours registered the maximum shoot length (21.63 cm). Kagzi lime seedlings raised from freshly extracted seeds resulted in higher collar diameter (2.04 mm) and sturdiness quotient (8.70). Further, a soaking duration of 12 hours proved better over 24 hours for collar diameter (2.04 mm) and sturdiness quotient (8.69). Between the different concentrations of GA3, 400 ppm gave better results for collar diameter (2.09 mm) and 200 ppm for sturdiness quotient (8.65).   Conclusion: Soaking freshly extracted kagzi lime seeds in an aqueous solution of 500 ppm GA3 for 12 hrs proved to be the best treatment combination for inducing early germination, higher Seedling Vigour Index and survival percentage. Nurserymen can employ these findings for early germination and vigorous seedlings in Kagzi lime. 

The potential of the smoke-derived compound 3-methyl-2H-furo[2,3-c]pyran-2-one as a priming agent for tomato seeds

2007 ◽  
Vol 17 (3) ◽  
pp. 175-181 ◽  
Author(s):  
Neeru Jain ◽  
Johannes Van Staden
Keyword(s):  
Large Scale ◽  
Seed Storage ◽  
Seed Priming ◽  
Stress Conditions ◽  
Scale Production ◽  
Seedling Vigour ◽  
Flow Cytometry Data ◽  
Tomato Seeds ◽  
Vigour Index ◽  
Continuous Presence

AbstractThe stimulatory role of 3-methyl-2H-furo[2,3-c]pyran-2-one, a smoke-derived butenolide, on germination and post-germination events is well documented. Previous studies have involved germinating seeds in the continuous presence of the compound. However, commercial growers cannot exploit the potential of the butenolide for large-scale production of crops due to limited availability and environmental constraints. The present study was undertaken to assess the potential of the butenolide as a priming agent of tomato (Solanum esculentum Mill.) seeds. Flow cytometry data revealed that butenolide-primed seeds had a higher percentage of nuclei at the 4C stage than water-primed seeds. Emergence of the radicle was much faster in the primed seeds. After 36 h of imbibition, a higher percentage of the butenolide-primed seeds (22%) exhibited radicle emergence compared to the water-primed seeds (12%). While butenolide-primed seeds initially germinated more rapidly than either water-primed or unprimed seeds in a 48-h period, water-imbibed seeds reached a similar germination level as the butenolide-primed seeds by 60 h of incubation. The butenolide-primed seeds produced significantly (P ≤ 0.05) more vigorous seedlings than water-primed seeds or seeds in the continuous presence of either water or butenolide. A gradual decrease in the seedling vigour index was recorded for both water and butenolide-primed seeds with increased seed storage at room temperature. Nevertheless, the vigour index was significantly greater in the butenolide-primed seeds than the water-primed seeds. Vigour indices were significantly (P ≤ 0.05) higher for the butenolide-primed seeds under various stress conditions (salinity, osmoticum or temperature) compared to control or water-primed seeds. Results of the present study suggest that the butenolide is a good seed-priming agent. Additionally, primed seeds retained the promotive effect for a considerable time. This was also the case for tomato seeds under various simulated field stress conditions.

scholarly journals The Influence Of Zinc On Seed Germination And Growth In The First Ontogenetic Stages In The Species Cucumis Melo L.

2015 ◽  
Vol 9 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Anişoara Stratu ◽  
Naela Costică
Keyword(s):  
Seed Germination ◽  
Cucumis Melo ◽  
Growth Process ◽  
Lateral Roots ◽  
Tolerance Index ◽  
High Concentration ◽  
Seedling Vigour ◽  
Ontogenetic Stages ◽  
Vigour Index ◽  
Germination And Growth

AbstractThe paper presents the results of a study regarding the influence of treatment with zinc in different concentrations (50 mg∕l, 100 mg∕l, 200 mg ∕l, 300mg/l, 400 mg ∕l, 500 mg/l, 600 mg ∕l) on seed germination and growth in early ontogenetic stages ofCucumis meloL. We analyzed the following indicators: the percentage of germinated seeds; the length of root, the length of the hypocotyl and the length of the seedling; the number of the laterale roots; the tolerance index and the seedling vigor index. The results underline the specific variations of analysed indicators, depending on the concentrations used for the treatments of seeds. The concentrations used for treatment do not influence negatively the seed germination, but affected the seedling growth (especially the root elongation), the formation and growth process of lateral roots and the seedling vigour index. The delay effect of growth process is very pronounced in the case of high concentration.

scholarly journals Size and vigor of Anadenanthera colubrina (Vell.) Brenan seeds harvested in Caatinga areas

2017 ◽  
Vol 39 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Jaciara de Souza Bispo ◽  
Danielle Carolina Campos da Costa ◽  
Samara Elizabeth Vieira Gomes ◽  
Gilmara Moreira de Oliveira ◽  
Janete Rodrigues Matias ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Dry Matter ◽  
Folk Medicine ◽  
Germination Percentage ◽  
Climate Variations ◽  
Randomized Design ◽  
Mother Plants ◽  
Physiological Quality ◽  
Completely Randomized Design

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.

scholarly journals Resistance to nematode Meloidogyne paranaensis in Arabica coffee genotypes introgressed with Coffea liberica

2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Mother Plants ◽  
Completely Randomized Design ◽  
Set Up ◽  
Moderately Resistant ◽  
Reproduction Factor ◽  
Coffea Liberica

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.

scholarly journals Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.)

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger
Keyword(s):  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Growth And Yield ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.

scholarly journals Effect of plant density and seed position on mother plant on physiological characteristic of cocklebur (Xanthium strumarium) seeds

2014 ◽  
Vol 32 (1) ◽  
pp. 61-68
Author(s):  
A. Foroughi ◽  
J. Gherekhloo ◽  
F. Ghaderi-Far
Keyword(s):  
Seed Quality ◽  
Plant Density ◽  
Physiological Characteristic ◽  
Mother Plant ◽  
Xanthium Strumarium ◽  
Randomized Design ◽  
Seed Position ◽  
Plant Densities ◽  
Shade Environment ◽  
Cardinal Temperatures

Experiments were conducted in 2010 to determine the influence of plant density and seed position on the mother plant on seed physiological characteristics of cocklebur (Xanthium strumarium). Cocklebur burs were collected in fall of 2010 from Research Farm of University of Agricultural Sciences and Natural Resources of Gorgan, Iran. The experiment was established as factorial arrangement using a completely randomized design with three replications. The factors included different densities of cocklebur (0, 2, 4, 6 and 8 plant m-2) and the top and bottom parts of the canopy. Non dormant seeds were used for determining cardinal temperatures and tolerance to salinity and drought stresses. Base, optimum and ceiling germination temperatures were estimated between 7.09 to 12.33, 32 to 35 and 44 to 45 respectively in different treatments. Salinity stress up to 300 Mm and osmotic potential 8 bar inhibited the germination completely. Comparison of base temperatures and sigmoid equation coefficients showed that seeds produced in the top had higher germination than those that produced at the bottom of the mother plant. It seems plant densities through seed position on the mother plant affect seed quality. Likewise changes of light quality and quantity in shade environment increased seed dormancy in matured seeds. Shade environment affect seed germination on mother plant that increased dormancy of seeds maturing under shade be an adaptive response that reduces the probability of germination of offspring under unfavorable (shade, competitive) conditions.

Influence of Different Pre-sowing Treatments on Germination and Seedling Vigour of Guava (Psedium guajava L.) Seeds

2015 ◽  
Vol 22 (1) ◽  
pp. 7-9
Author(s):  
Poomaruthai Masilamani ◽  
A. Nagaraja ◽  
M. Yadav ◽  
D. Srivastava
Keyword(s):  
Sulphuric Acid ◽  
Hot Water ◽  
Control Treatment ◽  
Seedling Vigour ◽  
Vigour Index ◽  
Index Value ◽  
And Control ◽  
Paper Method

The study aimed at investigating the effect of pre-sowing treatments on the germination and seedling vigour of Guava (Var. Allahabad Safeda) seeds. The pre- sowing treatments were made up cold and hot water soaking, manual scarification with sand paper, electronic scarification, different concentration of sulphuric acid, H2O2, CaOCl2 along with control. The treated and control seeds were placed for germination in top of the paper method. Twenty five days after sowing the result revealed that seeds soaked with CaOCl2 2% for 12 hrs. registered highest germination of 41 per cent followed by seed treated with H2SO4 200ml/kg for 15 minutes (40%). The control seeds gave only 10 per cent germination. Vigour index values shows significant differences among the treatments. The highest vigour index values of 119 were recorded in the seeds treated with H2SO4 200ml/kg for 10 minutes. The lowest vigour index value of 16 was recorded in control treatment. The results showed that treating the seeds with CaOCl2 2% for 12 hrs (or) treated with H2SO4 200ml/kg for 10 (or) 15 minutes was the most effective pre-sowing treatment for the germination and seedling vigour of Guava seeds.

Aptitudes germinatives comparées de graines de Rumex acetosella issues de populations correspondant à des stades distincts d'une succession postculturale

1988 ◽  
Vol 66 (7) ◽  
pp. 1381-1390 ◽  
Author(s):  
J. Escarré ◽  
C. Houssard
Keyword(s):  
Seed Weight ◽  
Plant Biomass ◽  
Germination Rate ◽  
The Other ◽  
Mother Plant ◽  
Old Fields ◽  
Rumex Acetosella ◽  
Significant Difference ◽  
Flowering Rate ◽  
Mother Plants

Germination of Rumex acetosella L. was studied under a variety of experimental conditions to determine whether populations from old fields of different ages (fallow for 6 months to 15 years) differed in seed weight and germination rate and in plant biomass and flowering rate of the resulting plants. Fresh seeds collected from these natural populations showed differences in germination that varied with the date of harvest and the number of years since the field was last cultivated: seeds of the youngest population harvested in July germinated the best and were lighter than those of the other populations. Different fertilizer levels applied during the growth of the mother plants from seeds collected in the original old fields affected the mean weight and the germination rate of the resulting seeds, regardless of the density of cultivation of the mother plant. However, the effect of different fertilizer levels on mean seed weight varied with the origin of each population. Heavy seeds (mean weight > 0.6 mg) of mother plants from the populations of fields abandoned over 2 years ago germinated better than light seeds (mean weight < 0.6 mg). There was no significant difference between heavy and light seeds in terms of percent germination for seeds from mother plants that came from the "youngest" field. These differences in germination rate observed in field-collected seeds were also found in light seeds of the offspring generation: light seeds of the mother plant from the population belonging to the more recently abandoned old field germinated the best. Plants grown from heavy seeds that came from the other populations of older fields had more biomass than those resulting from light seeds. This difference has not been observed between individuals resulting from light and heavy seeds of the youngest population. On the other hand, these individuals had a higher flowering rate than those resulting from the older populations. These results are interpreted in relation to the successional status of the populations: high flowering and early germination rates are suitable characteristics for establishment of plants on bare ground or after a disturbance, whereas heavy seeds with more reserves ensure germination and enough biomass of the resulting plants in density-dependent conditions.

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