Effects of Water Temperature on Activities of Metabolic Enzymes of Juvenile Siganus guttatus

Author(s):  
C. Song ◽  
Y. Wang ◽  
J.Y. Liu ◽  
F. Zhao ◽  
X.R. Huang ◽  
...  

Background: Temperature is one of the most important environmental factors affecting the survival, growth and metabolism of fish. The current study was aimed to study the effects of water temperature on the metabolic enzyme activities of juvenile Siganus guttatus. Methods: The juveniles were domesticated at 28±1°C for two weeks and then the temperature was adjusted to the target temperature groups (31°C, 27°C, 23°C and 19°C) by the gradually increasing or decreasing temperature with the change rate of 2°C per day. The experiment lasted for 70 d. At the end of the experiment, the fish were anesthetized and all the livers were dissected on ice plate and stored in the refrigerator at -80°C for the determination of enzyme activity. Result: The activities of glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT), hexokinase (HK) and pyruvate kinase (PK), lipoprotein lipase (LPL) and hepatic lipase (HL) tend to be increased with the reduction of temperature. The above enzymes activities in 19°C group were highest. The activity of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and citrate synthase (CS) was lowest in 19°C. These results suggests that 19°C had exceeded the suitable temperature range for juvenile S. guttatus. At low temperature, S. guttatus mainly use fat for energy, but less anaerobic metabolism for energy.

Author(s):  
Т.В. Никитина ◽  
А.А. Кашеварова ◽  
М.М. Гридина ◽  
А.А. Хабарова ◽  
А.Г. Мензоров ◽  
...  

Митотическая нестабильность кольцевых хромосом может приводить к появлению клеточных клонов с различной генетической структурой. В качестве модели нестабильности кольцевых хромосом в митозе мы использовали фибробласты от пациентов с r(8), r(13), r(18) и r(22) и полученные из них индуцированные плюрипотентные стволовые клетки (ИПСК). Линии ИПСК с r(22) имели относительно стабильный кариотип на протяжении десятков (до 60) пассажей и сохраняли неизменную структуру кольцевой хромосомы. Кариотип линий ИПСК с r(8) и r(18) на ранних пассажах стабильный, планируется его изучение на поздних пассажах. Наибольшее разнообразие кариотипа выявлено в линиях ИПСК с r(13), в которых наблюдали различные перестройки и выраженную клеточную гетерогенность. Определение факторов, влияющих на митотическую стабильность кольцевых хромосом, может иметь значение для консультирования пациентов. Mitotic instability of ring chromosomes can lead to the appearance of cell clones with different genetic structure. IPSCs from fibroblasts of patients with r(8), r(13), r(18), and r(22) were used as a model of ring chromosomes mitotic behavior. Karyotypes of iPSC lines with r(8) and r(18) have so far been evaluated only in the early passages, lines with r(22) have maintained a relatively stable karyotype up to 60 passages. The occurrence of rearrangements and cellular heterogeneity was found characteristic for r(13) iPSCs. The determination of factors affecting the ring chromosomes mitotic stability would be beneficial for the patient’s prognosis.


1992 ◽  
Vol 57 (8) ◽  
pp. 1632-1638 ◽  
Author(s):  
Věra Tatarkovičová ◽  
Zdeněk Stránský

The procedure for the determination of carbamate pesticides in soil was optimized. The following factors affecting the final results were investigated: extracting solvent, extraction procedure, extract purification procedure, and soil type. Triple extraction with acetone and purification of the extract on a two-stage purification column containing an activated carbon-silica gel 1+1 mixture were found optimal. The extracts after treatment were analyzed by RP-HPLC with UV detection. The method developed allows carbamate pesticides in soil to be determined at concentrations in excess of 30 μg kg-1.


2021 ◽  
Vol 22 (2) ◽  
pp. 348-363
Author(s):  
Damian Boniface Sambuo ◽  
Stephen Kirama ◽  
Kitala Malamsha

Determination of fish landing price is important, as the same contributes to the structure, conduct and performance of the fish market in Lake Victoria. Determination of relevant landing price is a gap to console between fishermen, agents (middlemen), processors and the government. The main objective of this study was therefore to examine fish price determination. Specifically, to examine the methods for fish price determination and analyse factors that affect fish landing price in Lake Victoria, a cross-sectional design was employed, and 300 respondents were randomly selected from two district councils, namely, Sengerema and Buchosa. Both qualitative and quantitative data were analysed using descriptive statistics and inferential analysis. Findings show that landing price is determined through formal negotiation with processors, consultation with other traders, informal negotiation with buyers and Beach Management Unit (BMU). The study concluded that these are the common methods used to determine landing prices. Also, distance from fishing to onshore landing centres, market information channels, age and experiences of the fishermen are the factors significantly found affecting landing price. It is recommended that the mechanism for setting up fishery price, fish market structure, fishery information and the formation of fishery regulatory body needs fishery policy and sector reforms that mark the determination of fish landing price.


2020 ◽  
Vol 9 (1) ◽  
pp. 170-181 ◽  
Author(s):  
Shangyong Zhang ◽  
Ruipeng Zhong ◽  
Ruoyu Hong ◽  
David Hui

AbstractThe surface activity of carbon black (CB) is an important factor affecting the reinforcement of rubber. The quantitative determination of the surface activity (surface free energy) of CB is of great significance. A simplified formula is obtained to determine the free energy of CB surface through theoretical analysis and mathematical derivation. The surface free energy for four kinds of industrial CBs were measured by inverse gas chromatography, and the influential factors were studied. The results showed that the aging time of the chromatographic column plays an important role in accurate measurement of the surface free energy of CB, in comparison with the influences from the inlet pressure and carrier gas flow rate of the chromatographic column filled with CB. Several kinds of industrial CB were treated at high temperature, and the surface free energy of CB had a significant increase. With the increase of surface free energy, the maximum torque was decreased significantly, the elongation at break tended to increase, the heat generation of vulcanizates was increased, and the wear resistance was decreased.


2021 ◽  
pp. 1-9
Author(s):  
Etsuo Niki

Reactive oxygen and nitrogen species have been implicated in the onset and progression of various diseases and the role of antioxidants in the maintenance of health and prevention of diseases has received much attention. The action and effect of antioxidants have been studied extensively under different reaction conditions in multiple media. The antioxidant effects are determined by many factors. This review aims to discuss several important issues that should be considered for determination of experimental conditions and interpretation of experimental results in order to understand the beneficial effects and limit of antioxidants against detrimental oxidation of biological molecules. Emphasis was laid on cell culture experiments and effects of diversity of multiple oxidants on antioxidant efficacy.


2020 ◽  
Vol 12 (1) ◽  
pp. 718-725
Author(s):  
Maria Mrówczyńska ◽  
Jacek Sztubecki ◽  
Małgorzata Sztubecka ◽  
Izabela Skrzypczak

Abstract Objects’ measurements often boil down to the determination of changes due to external factors affecting on their structure. The estimation of changes in a tested object, in addition to proper measuring equipment, requires the use of appropriate measuring methods and experimental data result processing methods. This study presents a statement of results of geometrical measurements of a steel cylinder that constitutes the main structural component of the historical weir Czersko Polskie in Bydgoszcz. In the initial stage, the estimation of reliable changes taking place in the cylinder structure involved the selection of measuring points essential for mapping its geometry. Due to the continuous operation of the weir, the points covered only about one-third of the cylinder area. The set of points allowed us to determine the position of the cylinder axis as well as skews and deformations of the cylinder surface. In the next stage, the use of methods based on artificial neural networks allowed us to predict the changes in the tested object. Artificial neural networks have proved to be useful in determining displacements of building structures, particularly hydro-technical objects. The above-mentioned methods supplement classical measurements that create the opportunity for carrying out additional analyses of changes in a spatial position of such structures. The purpose of the tests is to confirm the suitability of artificial neural networks for predicting displacements of building structures, particularly hydro-technical objects.


Mathematics ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 766
Author(s):  
Rashad A. R. Bantan ◽  
Ramadan A. Zeineldin ◽  
Farrukh Jamal ◽  
Christophe Chesneau

Deanship of scientific research established by the King Abdulaziz University provides some research programs for its staff and researchers and encourages them to submit proposals in this regard. Distinct research study (DRS) is one of these programs. It is available all the year and the King Abdulaziz University (KAU) staff can submit more than one proposal at the same time up to three proposals. The rules of the DSR program are simple and easy so it contributes in increasing the international rank of KAU. The authors are offered financial and moral reward after publishing articles from these proposals in Thomson-ISI journals. In this paper, multiplayer perceptron (MLP) artificial neural network (ANN) is employed to determine the factors that have more effect on the number of ISI published articles. The proposed study used real data of the finished projects from 2011 to April 2019.


Author(s):  
Amin Najafi ◽  
Mohammad Saeed Seif

Determination of high-speed crafts’ hydrodynamic coefficients will help to analyze the dynamics of these kinds of vessels and the factors affecting their dynamic stabilities. Also, it can be useful and effective in controlling the vessel instabilities. The main purpose of this study is to determine the coefficients of longitudinal motions of a planing catamaran with and without a hydrofoil using Reynolds-averaged Navier–Stokes method to evaluate the foil effects on them. Determination of hydrodynamic coefficients by experimental approach is costly and requires meticulous laboratory equipment; therefore, utilizing the numerical methods and developing a virtual laboratory seem highly efficient. In this study, the numerical results for hydrodynamic coefficients of a high-speed craft are verified against Troesch’s experimental results. In the following, after determination of hydrodynamic coefficients of a planing catamaran with and without foil, the foil effects on its hydrodynamic coefficients are evaluated. The results indicate that most of the coefficients are frequency-independent especially at high frequencies.


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