Comparative Body Condition and Haemato-biochemical Profile of Tellicherry Does during Pregnancy, Lactation and Dry Period

Author(s):  
T. Muthuramalingam ◽  
S. Meenakshi Sundaram ◽  
P. Tensingh Gnanaraj ◽  
T. Sivakumar ◽  
S. Vairamuthu ◽  
...  

Background: The aim of the study is to provide a complete picture of dynamics of body condition and selected hemato-biochemical parameters of Telicherry does during pregnancy, lactation and dry period.Methods: Blood samples were collected from 40 Tellicherry does ageing around 2-2½ years fortnightly during early pregnancy (1-3 months), late pregnancy (3-5 months), early lactation, mid lactation, late lactation and dry period. Haematological parameters such as haemoglobin (Hb), packed cell volume (PCV), red blood cells count (RBC) and white blood cells count (WBC) were analyzed. Biochemical parameters such as lactate dehydrogenase (LDH), blood urea nitrogen (BUN), total protein (TP), glucose, triglyceride, aspartate transaminase (AST), calcium (Ca), phosphorus (P), sodium (Na) and potassium (K) were estimated.Result: Significant reduction in body weight and condition was observed during early lactation followed by gradual increase during mid and late lactation. The levels of Hb, PCV, RBC (P less than 0.01) found to be elevated throughout pregnancy while WBC, BUN and total protein values were higher during late pregnancy and late lactation. However, LDH levels (P less than 0.01) were higher during both pregnancy and lactation. The serum levels of glucose and triglyceride were lowest during early lactation and highest during early pregnancy and dry period. Reduction in Ca and P (P less than 0.01) levels were observed throughout lactation. AST, Na and K levels remained unaffected during all the physiological status. 

2021 ◽  
Vol 52 (4) ◽  
pp. 941-948
Author(s):  
Awad & et al.

The present study designed  to investigate the hematological and blood biochemical changes in pre and post lambing periods in Iraqi Nuaemie ewes. Ten Nuaemie ewes weighed 35-45 kg and aged between 2-3 years were reared in animal's house of Veterinary College / Tikrit University from October-2018 to March-2019, Ten ml of blood samples were collected from each animal during the periods of last gestation month, at lambing and 2 weeks thereafter, Two and half ml of blood samples were collected in EDTA- containing tubes to determine the hematological parameters and the remaining was used to separate serum and stored at -20 °c for blood biochemical assessment. The results   revealed decreased in total red blood cells, haemoglobin and packed cell volume during post lambing period. The total white blood cells count and neutrophils were decreased during the post-partum period, while the lymphocyte was decreased at the day of lambing (50±5.8%). The biochemical parameters exhibited lesser total protein concentrations at the day of lambing (6.5± 1.85 g/dl ) while greater glucose, cholesterol and triglyceride concentrations during post-partum period. The concentration of urea and creatinine increased during the pre-partum period whereas, LDL and HDL concentrations increased in post- lambing period. The minerals concentrations revealed lesser concentrations of Zink and iron during the post-partum period while, copper concentration was greater during similar period. In conclusion, the physiological status of animals have clearly effects on the haematological and biochemical parameters in Iraqi Nuaemie ewes.  


Author(s):  
Y. Garba ◽  
E. A. Adeola

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.


2015 ◽  
Vol 67 (2) ◽  
pp. 535-545 ◽  
Author(s):  
Marko Prokic ◽  
Milica Paunovic ◽  
Milos Matic ◽  
Natasa Djordjevic ◽  
Branka Ognjanovic ◽  
...  

Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH), as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC) was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO) and lipid peroxides (LPO) in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases.


animal ◽  
2019 ◽  
Vol 13 (7) ◽  
pp. 1478-1488 ◽  
Author(s):  
K. Schuh ◽  
H. Sadri ◽  
S. Häussler ◽  
L.A. Webb ◽  
C. Urh ◽  
...  

2014 ◽  
Vol 11 (2) ◽  
pp. 3-6
Author(s):  
B Bayar-Enkh ◽  
S Ganbat ◽  
P Enkhtuya

Some hematological values, blood total protein, protein fractions, and minerals such as calcium and phosphorus of takhi, which are now being reintroduced in Khustain natural park, were measured. There were 8.3 million red blood cells and 8000 white blood cells in 1 mm3 whole blood of takhi reintroduced in Khustain natural park, and hemoglobin was 179.6±3.2 g/l. As well, total proteins, calcium and phosphorus of takhi were 67± 0.6 g/l, 2.1±0.31 mmol/l, and 1.1±0.2 mmol/l respectively and they were similar to those in Mongolian horses. It has been necessary to investigate further adaptability of takhi in asscoation with both internal and external environments of its body.DOI: http://dx.doi.org/10.5564/mjas.v11i2.206 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.3-6


Author(s):  
M. J. Argente ◽  
D. M. Abad-Salazar ◽  
E. M. Bermejo-González ◽  
M. L. Garcíaz ◽  
A. López-Palazón

Rabbit is widely used as an experimental animal model in infectious and non-infectious diseases. The haematologic data can be helpful in evaluating the health status of animals over time. The aim of this study was to determine the levels of red blood cells (RBC), white blood cells (WBC) and differential leukocyte counts in 5 nulliparous and 5 multiparous females, i.e. in young and older animals, at mating and at delivery. The values of RBC did not change with age, but WBC and lymphocytes decreased with age, a -33% and a -60% less in multiparous females than nulliparous ones. Monocytes count was double at delivery than at mating. In conclusion, aging on the immune system is manifested as reduction in production of mature lymphocytes and as a result, older females would not respond to immune challenge as robustly as the young ones. Physiological status is only related to production of monocytes.


2017 ◽  
Vol 63 (12) ◽  
pp. 1856-1865 ◽  
Author(s):  
Christopher R McCudden ◽  
John Brooks ◽  
Priya Figurado ◽  
Pierre R Bourque

Abstract BACKGROUND Reference intervals are vital for interpretation of laboratory results. Many existing reference intervals for cerebrospinal fluid total protein (CSF-TP) are derived from old literature because of the invasive nature of sampling. The objective of this study was to determine reference intervals for CSF-TP using available patient data. METHODS Twenty years of hospital database information was mined for previously reported CSF-TP results. Associated demographic, laboratory, and clinical diagnosis (International Classification of Diseases 9/10 codes) details were extracted. CSF-TP results included 3 different analytical platforms: the Siemens Vista 1500, Beckman Lx20, and Roche Hitachi 917. From an initial data set of 19591 samples, the following exclusion criteria were applied: incomplete data, white blood cells (WBCs) &gt;5 × 106/L, red blood cells (RBCs) &gt;50 × 106/L, and glucose &lt;2.5 mmol/L. Patient charts were reviewed in detail to exclude 60 different conditions for which increases in CSF-TP would be expected. A total of 6068 samples were included; 63% of the samples were from females. Continuous reference intervals were determined using quantile regression. Age- and sex-partitioned intervals were established using the quantile regression equation and splitting age-groups into 5-year bins. RESULTS CSF-TP showed a marked age dependence, and males had significantly higher CSF-TP than females across all ages. CSF-TP results from the 3 different instruments and manufacturers showed small (approximately 0.04 g/L), but statistically significant, differences. CSF-TP showed weak, but again statistically significant, correlation with WBC and RBC but was independent of serum total protein and creatinine. CONCLUSIONS The age dependence of CSF-TP supports that age-partitioned reference intervals will be more accurate than a single cutoff, particularly in patients with advancing age.


2020 ◽  
Vol 17 (1(Suppl.)) ◽  
pp. 0207
Author(s):  
Safaa Ali

This study is conducted to evaluate the therapeutic and antioxidant effect of lemon juice on some hematological and biochemical parameters. Thirty female mice used in this study were exposed to oxidative stress through giving them hydrogen peroxide in drinking water for 30 days. Animals randomly distributed over 3 groups, each group contained 10 animals and treated as follows: T1 control group (drinking distilled water only), T2 (0.75% hydrogen peroxide in drinking water) and T3 (0.75% hydrogen peroxide in drinking water with daily drenching with 1 mL lemon juice). At the end of the experiment, blood samples were collected from animals for evaluating the following hematological and biochemical parameters: Haemoglobin concentration (Hb), red blood cells count (RBC), white blood cells count (WBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), level of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total protein. The results showed that T3 exhibited an enhancement in RBC count, Hb concentration, WBC, lymphocyte and total protein and reduction in the level of AST and ALT compared to T2. These findings clearly revealed the advance protective and antioxidant features of lemon juice on hematological and biochemical parameters of the oxidatively stressed female mice.


2017 ◽  
Vol 48 (2) ◽  
Author(s):  
O. S. H. AL-jubori

This study was aimed to find out the synergistic effect of ginger and propolis some productivity and physiological traits of Awassi ewes. The study was conducted in field the Department of Animal Production - College of Agriculture, University of Baghdad Al-Jaderia, during 14/01/2016 - 03/27/2016, using 12 Awassi ewes with ages from 9 -11 months old ,average weight of 31 kg, The results showed a significant increase in the glucose for concentration (P <0. 05) in the ginger group compared with other groups where .significant increase in the concentration of blood sugar(81.0 mg / 100 ml of blood)  in the first month and continued to the second month,The level of cholesterol increase (65.90) in the second month of the group of ginger, triglycerides, Low-density lipid had significant increase in the group ginger , significant increase in  propolis group with high density lipid (54.00) , total protein , albumin and GPT enzyme shows significant increased effect on the contrary GOT enzyme was increased significant in mixture group in the second month (13.00IU / liter), while the level of hemoglobin and the Packed cells volume and the total count of white blood cells and red blood cells did not show any significant differences(normal value ) , It can be conclude from this study that the addition of 25 g ginger to concentrated diet / head / day is the best treatment.


2014 ◽  
Vol 57 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Martina Bedenicki ◽  
Dalibor Potocnjak ◽  
Ivica Harapin ◽  
Berislav Radisic ◽  
Marko Samardzija ◽  
...  

Abstract. Haematological and biochemical parameters were examined in the blood of 87 cows, bulls and oxen of the indigenous Croatian breed, Istrian cattle. The sample represents 11.93 % of the total adult population. This breed is classified as highly endangered. The age of tested animals was between 2 and 17 years. Cattle were divided into three groups, animals aged 2 to 6 years (n=19), 7 to 10 years (n=37), and older than 10 years (n=31). Animals were kept in the area of Istria County, mostly in stables though occasionally at pasture. The cows were milked twice daily and gave an average of 1 000 litres of milk per year. Erythrocytes, leukocytes, haemoglobin, haematocrit and mean corpuscular volume were determined. Differential analysis was performed by counting blood cells in the blood smear after Pappenheim staining. Biochemical parameters such as total cholesterol, triglycerides, urea, creatinine, total bilirubin, calcium and inorganic phosphorus were determined. The activity of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase was determined by spectrophotometry. These biochemical and haematological parameters of this Istrian breed were within the physiological range, while slight deviations were observed for certain parameters. The greatest deviation from the physiological range was shown in the concentrations of urea and creatinine. These results may contribute to a better understanding of the metabolic profile and haematological indicators for estimating the physiological status of these endangered cattle in Istria, for further investigation and for diagnostic purposes.


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