Antimicrobial activity of three medicinally important plants (Berberis balochistanica, Thymus serpyllum and Salvia bucharica) of Shudden, Mazhoo Tehsil Nana Sab area, District Pishin, Balochistan-Pakistan

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

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The content and antimicrobial activity of essential oils in the herb Thymus marshallianus Willd. and Thymus serpyllum L.

Problems of Biological Medical and Pharmaceutical Chemistry ◽

10.29296/25877313-2021-03-04 ◽

2021 ◽

Vol 24 (3) ◽

pp. 27-32

Author(s):

A.S. Sheremetyeva ◽

A.V. Frolkova ◽

O.G. Shapoval ◽

N.A. Durnova ◽

M.A. Berezutsky

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Thymus Serpyllum

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Antimicrobial Activity of Ultrasonic Extracts of Two Chemotypes of Thymus serpyllum L. of Central Kazakhstan and their Polyphenolic Profiles

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.5520 ◽

2021 ◽

Vol 9 (A) ◽

pp. 61-67

Author(s):

Svetlana Ivasenko ◽

Perizat Orazbayeva ◽

Krystyna Skalicka–Wozniak ◽

Agnieszka Ludwiczuk ◽

Alexandr Marchenko ◽

...

Keyword(s):

Antimicrobial Activity ◽

Wide Spectrum ◽

Biological Properties ◽

Climatic Conditions ◽

Biologically Active ◽

Gram Positive Bacteria ◽

Quantitative Content ◽

Two Samples ◽

Thymus Serpyllum ◽

Bacteria And Fungi

BACKGROUND: The medicinal plant of Thymus serpyllum L. in nature, depending on the geographical region, climatic conditions, and growing environment, is represented with some chemotypes. Composition and quantitative content of the basic groups of the biologically active substances can be differed, and thus their biological properties are also various. AIM: The aim of the study was to determine possibility of the using the ultrasonic extracts of two chemotypes of T. serpyllum L. of Central Kazakhstan as an antimicrobial agent against test strains of microorganisms. MATERIALS AND METHODS: Two samples of T. serpyllum were extracted with 70% ethanol using ultrasound. The polyphenol content of the ultrasound extracts was determined using the LC-ultraviolet-ESI- tandem mass spectrometry technique. A study of an antimicrobial activity of the ultrasonic extracts was performed with eight strains of Gram-positive bacteria, six strains of Gram-negative bacteria, and four cultures of fungi. RESULTS: The ultrasonic extracts of two chemotypes of T. serpyllum L. are similar in composition of phenolic compounds but differ in a quantitative content of phenolic acids and flavonoids, except for a rosmarinic acid. The ultrasonic extracts have a wide spectrum of antimicrobial activity, exhibit the bactericidal or bacteriostatic activity against all tested bacteria and fungi at a concentration of 0.0625–20 mg/ml, but differ in their strength of action against test strains of microorganisms. CONCLUSION: The ultrasonic extracts of two chemotypes of T. serpyllum L. of Central Kazakhstan can be considered as a potential drug with a wide spectrum of antimicrobial activity. The results of chromatographic analysis will be used for standardization of a drug.

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Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha.43.2.9899 ◽

2015 ◽

Vol 43 (2) ◽

Cited By ~ 1

Author(s):

Aneta WESOŁOWSKA ◽

Monika GRZESZCZUK ◽

Dorota JADCZAK ◽

Paweł NAWROTEK ◽

Magdalena STRUK

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Thymus Serpyllum

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Antimicrobial activity of extracts of Thymus marschallianus and Thymus serpyllum against clinical strains of microorganisms

Традиционная медицина ◽

10.54296/18186173_2021_3_27 ◽

2021 ◽

pp. 27-32

Author(s):

A.S. Sheremetyeva ◽

O.G. Shapoval ◽

A.V. Frolkova ◽

N.A. Durnova

Keyword(s):

Antimicrobial Activity ◽

Clinical Strains ◽

Thymus Serpyllum

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HPLC-DAD analysis of Thymus serpyllum based natural pigments and investigation of their antimicrobial properties

Pigment & Resin Technology ◽

10.1108/prt-07-2012-0045 ◽

2013 ◽

Vol 43 (1) ◽

pp. 19-25 ◽

Cited By ~ 2

Author(s):

Emrah Çakmakçi ◽

Ozan Deveoglu ◽

Ahmed Muhammed ◽

Ali Fouad ◽

Emine Torgan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Thermal Degradation ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Precipitation Method ◽

Degradation Behaviour ◽

Content Type ◽

Natural Pigments ◽

Thymus Serpyllum

Purpose – In this study, it was aimed to investigate the antibacterial properties of natural pigments prepared from Thymus serpyllum. Design/methodology/approach – Al (III), Fe (II), Sn (II) and Cu (II) complexed natural pigments were obtained by using a precipitation method and the main constituents in the pigments were identified with HPLC-DAD. Also FTIR analysis was performed for further structural characterization. Moreover, the thermal stability and thermal degradation properties of the pigments were analyzed by thermogravimetric analyses (TGA). The antimicrobial activity of the thyme plant-extracted pigments was evaluated by measuring the minimal inhibitory concentration. Findings – Apigenin and luteolin flavones were detected as the main components of the natural dyes. Thermal degradation behaviour of the pigments was determined by means of TGA. All pigments showed high char yields and it was attributed to the high complexation between the metal and the ligand species. The antimicrobial activity of the thyme plant-extracted pigments was measured and it was found that all pigments had high antimicrobial activity. Aluminum-thymus pigments showed the highest antimicrobial efficiency among other pigments used in this study. Originality/value – The obtained pigments have high antimicrobial activities, and therefore, they can be used for the production of antimicrobial textiles. Furthermore, Thymus-based natural pigments might have potential applications in coating, paint, plastic industries, etc.

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Antimicrobial Activity of Antibiotics, Oregano and Thyme Essential Oils against Extended Spectrum Beta Lactamase Producing Klebsiella pneumoniae Clinical Isolates

Journal of Advances in Microbiology ◽

10.9734/jamb/2021/v21i1230419 ◽

2021 ◽

pp. 112-119

Author(s):

Snježana Hodžić ◽

Amela Hercegovac ◽

Nijaz Tihić ◽

Darja Husejnagić ◽

Aldijana Avdić ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Klebsiella Pneumoniae ◽

Essential Oils ◽

Clinical Isolates ◽

Diffusion Method ◽

Beta Lactamase ◽

Extended Spectrum Beta Lactamase ◽

Extended Spectrum ◽

Thymus Serpyllum

Aims: The aims of this study was to investigate the susceptibility of extended-spectrum beta-lactamase (ESBL) producing Klebsiella pneumoniae clinical isolates to antibiotics and essential oils - Origanum compactum, Origanum majorana and Thymus serpyllum. Study Design: Study included 30 isolates of Klebsiella pneumoniae obtained from clinical material provided from the University Clinical Center Tuzla. Place and Duration of Study: Department of Biology, Faculty of Science, University of Tuzla, BiH, between September 2019 to September 2020. Methodology: Antibiotic susceptibility testing was performed by the Kirby-Bauer disk diffusion method. The following commercially available antibiotic discs were used: amoxicillin (30µg), cefalexin (30 µg), gentamicin (10 µg), amikacin (30 µg), imipenem (10 µg), piperacillin (75µg), ampicilin (10 µg), meropenem (10 µg), ciprofloksacin (10 µg), ceftazidim (30 µg), cefotaksim (30 µg), ceftriaxone (30 µg), cefepime (30 µg) and aztreonam (30 µg). The antibacterial effect of the essential oils was tested for ESBL K. pneumoniae isolates using the diffusion method according to Clinical laboratory standards institute (CLSI) guidelines. Results: O. compactum and O. majorana essential oils showed the same antimicrobial activity with 80.0% effect on ESBL K. pneumoniae isolates, Thymus serpyllum EO showed antimicrobial activity of 60.0%. The lowest MIC value had the O. compactum essential oil (MIC 6 mg/ml-10.5 mg/ml), followed by the T. serpyllum (MIC 17.2 mg/ml-43 mg/ml), while the O. majorana essential oil showed MIC values in range from 11 mg/ml to 39 mg/ml. Conclusion: The results of the study showed the exceptional sensitivity of ESBL K. pneumoniae clinical isolates to the essential oils from Origanum and Thymus genera, which highly suggests their potential application in the struggle against these pathogens in the future.

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Chemical Characterization and Antibacterial Activity of Essential Oil of Medicinal Plants from Eastern Serbia

Molecules ◽

10.3390/molecules25225482 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5482

Author(s):

Milica Aćimović ◽

Miroslav Zorić ◽

Valtcho D. Zheljazkov ◽

Lato Pezo ◽

Ivana Čabarkapa ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Chemical Characterization ◽

Bacterial Strains ◽

Origanum Vulgare ◽

Medicinal Species ◽

Self Medication ◽

Pharmaceutical Industries ◽

Thymus Serpyllum ◽

The Village

The objective of this study was to evaluate wild growing Satureja kitaibelii, Thymus serpyllum, Origanum vulgare, Achillea millefolium and Achillea clypeolata with respect to their essential oil (EO) content, composition and antimicrobial activity. The five species were collected at Mt. Rtanj and the village of Sesalac, Eastern Serbia. The main EO constituents of Lamiaceae plants were p-cymene (24.4%), geraniol (63.4%) and germacrene D (21.5%) in Satureja kitaibelii, Thymus serpyllum and Origanum vulgare ssp. vulgare, respectively. A. millefolium EO had multiple constituents with major ones being camphor (9.8%), caryophyllene oxide (6.5%), terpinen-4-ol (6.3%) and 1,8-cineole (5.6%), while the main EO constituents of A. clypeolata were 1,8-cineole (45.1%) and camphor (18.2%). Antimicrobial testing of the EO showed that Staphylococcus aureus (Gram-positive) was more sensitive to all of the tested EOs than Escherichia coli (Gram-negative). S. kitaibelii EO showed the highest antimicrobial activity against both tested bacterial strains. This is the first study to characterize the EO composition and antimicrobial activity of these five medicinal species from Eastern Serbia in comparison with comprehensive literature data. The results can be utilized by the perfumery, cosmetics, food and pharmaceutical industries, but also for healing purposes in self-medication.

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