scholarly journals Whitening and Moisturizing Effects of Hydrolyzed Swiftlet Nest Extracts

2021 ◽  
Vol 19 (4) ◽  
pp. 639-649
Author(s):  
Yun Jeong Kim ◽  
Ye Sol Goh ◽  
Waiting Cheung ◽  
Yong Sam Kim ◽  
Hyunsang Lee
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw Material ◽  
Dependent Manner ◽  
Dpph Radical ◽  
Concentration Dependent Manner ◽  
Skin Whitening ◽  
Melanin Contents ◽  
Dpph Radical Scavenging

Purpose: This research verified the skin whitening and moisturizing effects of hydrolyzed swiftlet nest extracts (HSNE) in vitro using human keratinocytes and melanoma.Methods: To confirm the antioxidant effect of HSNE, DPPH radical-scavenging activity was measured. To find out the whitening effect of HSNE, the genes related to melanogenesis, mRNA expression of tyrosinase (TYR), tyrosinase related protein (TRP) 1, 2 and microphthalmia associated transcription factor (MITF) were measured. We also measured the melanin contents after treatment of HSNE to confirm the anti-melanogenesis effect. Using reverse transcription polymerase chain reaction (RT-PCR), the genes related to moisturizing such as aquaporin (AQP) 3, hyaluronan synthase (HAS) 1, 2, and 3 were determined. The results of the tests were analyzed with student’s t-test and expressed as mean±standard deviation.Results: DPPH radical scavenging effects of HSNE increased in a concentration dependent manner. The expression of melanogenesis-related genes were inhibited by the treatment of HSNE in a concentration-dependent manner (MITF, TYR, TRP1, and 2). Melanin contents also decreased with the treatment of HSNE. The expression of moisturizing-related genes (HAS1, 2, 3, and AQP3) increased in a concentration-dependent manner.Conclusion: It is confirmed that the hydrolyzed swiftlet nest extracts have skin whitening and moisturizing effects and can be used as a functional cosmetic raw material.

scholarly journals In Vitro Toxicity, Antioxidant, Anti-Inflammatory, and Antidiabetic Potential of Sphaerostephanos unitus (L.) Holttum

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 333 ◽  
Author(s):  
Marimuthu Alias Antonisamy Johnson ◽  
C. Xavier Madona ◽  
Ray S. Almeida ◽  
Natália Martins ◽  
Henrique D. M. Coutinho
Keyword(s):  
Petroleum Ether ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Toxicity ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Pteridophytes have been widely used in several systems of medicine. Several reports have increasingly assessed their bioactive effects, but for Sphaerostephanos unitus (L.) Holttum, only its antibacterial potential has been assessed. In this sense, the present study was carried out to reveal the phytochemical profile and to determine the toxicity, antioxidant, antidiabetic, and anti-inflammatory potential of S. unitus. Brine shrimp lethality, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, phosphomolybdenum assay, superoxide radical scavenging activity, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assay (ABTS), and in vitro α-amylase inhibitory and membrane stabilization assays were applied. S. unitus extract toxicity showed variable mortality percentages, with LC50 values ranging from 4 to 30 mg/mL. DPPH radical scavenging effects of S. unitus extracts were as follows: methanol > acetone > petroleum ether > chloroform. S. unitus acetone extract displayed the strongest phosphomolybdenum reduction (10 ± 2 mg Ascorbic Acid Equivalent/g). The studied extracts also revealed efficient, superoxide scavenging effects in a dose-dependent manner. In S. unitus, the highest ABTS radical scavenging rate was observed in the chloroform extract (3000 ± 40 µmol/g). The S. unitus anti-inflammatory effect was as follows: petroleum ether > chloroform > methanol > acetone. In S. unitus extract, the highest percentage of α-amylase activity (80%) was observed for the petroleum ether extract (25 µg/mL). Faced with these findings, further studies should be performed to isolate and identify the S. unitus compounds responsible for their antioxidant, antidiabetic and anti-inflammatory effects.

IN VITRO ANTIOXIDANT AND ALPHA-AMYLASE, ALPHA-GLUCOSIDASE INHIBITORY ACTIVITY OF FLOWERS OF CALOTROPIS GIGANTEA

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (10) ◽  
pp. 38-42
Author(s):  
N. K Choudhary ◽  
◽  
J Dwivedi ◽  
S Sharma
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Antidiabetic Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The present investigations were carried out to evaluate the in vitro antioxidant as well as antidiabetic activity of flowers of Calotropis gigantea. Different extracts (petroleum ether, chloroform and ethanolic extract) were prepared using successive solvent extraction method (soxhlet) and screened for its in vitro antioxidant activity using Diphenyl picryl hydrazyl (DPPH) radical scavenging activity, ABT S radical cation decolorization assay and nitric oxide (NO) radical scavenging activity and IC50 were calculated. In vitro antidiabetic activity was studied using α – amylase and α – glucosidase inhibitory assay. Chloroform extract, among the three extracts (i.e. petroleum ether, chloroform and ethanolic), showed maximum antioxidant activity with IC50 value of 151.23µg/ml, 73.56 µg/ml and 158.92µg/ml against DPPH radical scavenging activity, ABTS radical cation decolorization assay and nitric oxide (NO) radical scavenging activity respectively. The chloroform extract produced a significant in vitro antidiabetic activity with IC50 of 52.3µg/ml 18.2µg/ml against α – amylase and α – glucosidase enzymes but less inhibitory effect than standard acarbose.

scholarly journals In Vitro Antioxidant Activity of Dibenz[b,f]azepine and its Analogues

2008 ◽  
Vol 5 (s2) ◽  
pp. 1123-1132 ◽  
Author(s):  
H. Vijay Kumar ◽  
C. R. Gnanendra ◽  
Nagaraja Naik ◽  
D. Channe Gowda
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Antioxidant Compounds ◽  
Dependent Manner ◽  
Concentration Dependent Manner

Dibenz[b,f]azepine and its five derivatives bearing different functional groups were synthesized by known methods. The compounds thus synthesized were evaluated for antioxidant potential through different in vitro models such as (DPPH) free radical scavenging activity,ß-carotene-linoleic acid model system, reducing power assay and phosphomolybdenum method. Under our experimental condition among the synthesized compounds dibenz[b,f]azepine (a) and 10-methoxy-5H-dibenz[b,f]azepine (d) exhibited potent antioxidant activity in concentration dependent manner in all the above four methods. Butylated hydroxyl anisole (BHA) and ascorbic acid (AA) were used as the reference antioxidant compounds. The most active compounds like dibenz[b,f]azepine and its methoxy group substituent have shown more promising antioxidant and radical scavengers compared to the standards like BHA and ascorbic acid. It is conceivable from the studies that the tricyclic amines,i.e. dibenz[b, f]azepine and some of its derivatives are effective in their antioxidant activity properties.

scholarly journals In Vitro Antidiabetic Effects and Antioxidant Potential ofCassia nemophilaPods

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Gauhar Rehman ◽  
Muhammad Hamayun ◽  
Amjad Iqbal ◽  
Saif Ul Islam ◽  
Saba Arshad ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Yeast Cells ◽  
In Vitro Assays ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

The antidiabetic and antioxidant potential of ethanolic extract ofCassia nemophilapod (EECNP) was evaluated by three in vitro assays, including yeast glucose uptake assay, glucose adsorption assay, and DPPH radical scavenging activity. The result revealed that the extracts have enhanced the uptake of glucose through the plasma membrane of yeast cells. A linear increase in glucose uptake by yeast cells was noticed with gradual increase in the concentration of the test samples. Moreover, the adsorption capacity of the EECNP was directly proportional to the molar concentration of glucose. Also, the DPPH radical scavenging capacity of the extract was increased to a maximum value of 43.3% at 80 μg/ml, which was then decreased to 41.9% at 100 μg/ml. From the results, it was concluded that EECNP possess good antidiabetic and antioxidant properties as shown by in vitro assays.

scholarly journals Evaluation of Ethnopharmacological and Antioxidant Potential ofZanthoxylum armatumDC.

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Rabia Kanwal ◽  
Muhammad Arshad ◽  
Yamin Bibi ◽  
Saira Asif ◽  
Sunbal Khalil Chaudhari
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Zanthoxylum Armatum

Zanthoxylum armatumDC. (syn.Z. alatumRoxb.) is an important medicinal plant commonly called Timur or Indian prickly ash. The ethnopharmacological study ofZ. armatumrevealed the use of different plant parts for curing various ailments including cholera, chest infection, fever, indigestion, stomach disorders, gas problems, piles, toothache, gum problems, dyspepsia, as carminative, antipyretic, aromatic, tonic, and stomachic. Keeping in view the medicinal potential of the plant, the antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, and phosphomolybdate assay using different concentrations (7.81 μg/mL–250 μg/mL). Ascorbic acid was taken as standard. The results indicated that the free radical scavenging activity ranged from 40.12% to 78.39%, and the reductive potential ranged from 0.265 nm to 1.411 nm while the total antioxidant activity ranged from 0.124 nm to 0.183 nm. The antioxidant potential evaluated by three assays increased in a concentration dependent manner and ascorbic acid showed better antioxidant activity than leaf extract. Results obtained through different tests confirmed redox protective activities ofZanthoxylum armatum. Further in vitro and in vivo research should be performed, so this plant can be further utilized in drug development.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF CORCHORUS OLITORIUS

2017 ◽  
pp. 59-63
Author(s):  
Abdul Sadat ◽  
Mayukh Hore ◽  
Kaushik Chakraborty ◽  
Subhrajyoti Roy
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

scholarly journals IN VITRO SCREENING OF ANTIOXIDANT AND ANTIAGING POTENTIAL OF CUCUMIS SATIVUS FRUIT EXTRACT

2020 ◽  
pp. 187-190
Author(s):  
CHANCHAL GARG ◽  
RAVINDER SINGH ◽  
MUNISH GARG
Keyword(s):  
Ethyl Acetate ◽  
Cucumis Sativus ◽  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Objective: The present study was designed to screen the anti-aging and anti-wrinkle potential of Cucumis sativus fruit through in vitro estimation of antioxidant, anti-hyaluronidase, anti-elastase, anti-collagenase/anti-matrix metalloproteinase (MMP)-1, and anti-tyrosinase activity. Methods: Raw juice of cucumber was taken, filtered and fractionated with ethyl acetate and n-butanol. The obtained extracts were then evaluated for their antioxidant potential through 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay taking ascorbic acid as positive control and other enzymatic activities in reference to hyaluronidase inhibition, MMP-1/collagenase inhibition, and elastase inhibition taking catechin as reference standard whereas for tyrosinase inhibition the standard used was quercetin. Results: All the evaluations were performed in triplicates and results were noted down. It was observed that aqueous extract of C. sativus fruits showed a maximum DPPH radical scavenging activity (p<0.0001), half-maximal inhibitory concentration (IC50) at a concentration of 122.67 μg/ml. The ethyl acetate fraction of C. sativus fruits exhibited maximum hyaluronidase (p<0.0001), MMP-1/collagenase (p<0.04), and tyrosinase (p<0.04) inhibitory activity, IC50 at a concentration of 59.54, 45.79, and 24.46 μg/ml, respectively. The elastase (p<0.0001) inhibitory activity by n-butanol fraction of C. sativus fruits extract was maximum, IC50 at a concentration of 52.76 μg/ml. Conclusion: A potent anti-aging and anti-wrinkle properties were well demonstrated by C. sativus, as depicted from the results obtained.

scholarly journals EFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS

2020 ◽  
pp. 128-131
Author(s):  
RAMBU KONDA A PRAING ◽  
TITIK SUNARNI
Keyword(s):  
Antioxidant Activity ◽  
Glutathione Peroxidase ◽  
Wistar Rats ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Gpx Activity

Objective: The aims of the research were to evaluate the antioxidant effect of Sterculia quadrifida R.Br bark by in vitro 1,1-biphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method and glutathione peroxidase (GPx) activity on diabetic Wistar rats model. Methods: S. quadrifida bark powder was extracted in ethanol 70% by maceration method. The antioxidant activity of ethanol extract was tested by DPPH radical scavenging method. The activity of GPx was evaluated from the liver of alloxan-induced diabetic rats. The antioxidant activity was determined by measuring the absorbance using ultraviolet-visible spectrophotometry method. Results: The ethanol extract of S. quadrifida bark had a strong DPPH radical scavenging activity, with the IC50 value was 4.86±0.01 ug/ml, besides that the extract also showed significantly activity of GPx (p<0.05) at a dose of 65, 130, and 260 mg/kg bw. Conclusion: The ethanol extract of S. quadrifida bark had a potent antioxidant activity by DPPH radical scavenging and GPx activity.

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