scholarly journals Produksi Enzim Fibrinolitik Tempe oleh Rhizopus oryzae FNCC 6078

2021 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Alicia Sada ◽  
Noor Erma Sugianto ◽  
Achmad Toto Poernomo
Keyword(s):  
Solid State ◽  
Optimum Condition ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Fibrinolytic Activity ◽  
Rhizopus Oryzae ◽  
Fibrinolytic Enzyme ◽  
Temperature Optimum ◽  
Inoculum Volume

ackground Rhizopus oryzae FNCC 6078 had been evaluated producing fibrinolytic enzyme under solid state fermentation. Soybean had been used to produce fibrinolytic enzyme through fermentation in tempeh. The main purpose of this study was to reveal optimum condition for fermentation. The parameters of the condition were inoculum volume, incubation period and temperature. Optimum condition was defined by maximum fibrinolytic activity. Methode Fibrinolytic activity was measured using spectrophotometry at 274 nm. Result optimum condition for producing fibrinolytic enzyme was 1,5 mL volume of inoculum of Rhizopus oryzae suspension in 25%T, 42 hours for incubation period and 35oC temperature incubation.

Lignocellulolytic activity of Pleurotus ostreatus under solid state fermentation using silage, stover, and cobs of maize

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3797-3807
Author(s):  
Magdah Ganash ◽  
Tarek M. Abdel Ghany ◽  
Mohamed A. Al Abboud ◽  
Mohamed M. Alawlaqi ◽  
Husam Qanash ◽  
...  
Keyword(s):  
Solid State ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
Value Added ◽  
Extracellular Protein ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulosic Substrate

Lignocellulolytic white-rot fungi allow the bioconversion of agricultural wastes into value-added products that are used in a myriad of applications. The aim of this work was to use corn residues (Zea mays L.) to produce valuable products under solid-state fermentation (SSF) with Pleurotus ostreatus. White-rot fungus P. ostreatus was isolated from maize silage (MS) and thereafter it was inoculated on MS as substrate and compared with maize stover (MSt) and maize cobs (MC) to determine the best lignocellulosic substrate for the production of lignocellulolytic enzymes and extracellular protein. The MS gave the highest productivity of CMCase (368.2 U/mL), FPase (170.5 U/mL), laccase (11.4 U/mL), and MnPase (6.6 U/mL). This is compared to productivity on MSt of 222 U/mL, 50.2 U/mL, 4.55 U/mL, and 2.57 U/mL, respectively; and productivity on MC at the same incubation period as 150.5 U/mL, 48.2 U/mL, 3.58 U/mL, and 2.5 U/mL, respectively. The levels of enzyme production declined with increasing incubation period after 15 and 20 days using MS and MC, respectively, as substrates. Maximum liberated extracellular protein content (754 to 878 µg/mL) was recorded using MS, while a low amount (343 to 408 µg/mL) was liberated with using MSt and MC.

scholarly journals Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme byBacillussp. IND12 Using Response Surface Methodology in Solid-State Fermentation

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Ponnuswamy Vijayaraghavan ◽  
P. Rajendran ◽  
Samuel Gnana Prakash Vincent ◽  
Arumugaperumal Arun ◽  
Naif Abdullah Al-Dhabi ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Solid State ◽  
Response Surface ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Blood Clot ◽  
Fibrinolytic Enzyme ◽  
Surface Model ◽  
Cow Dung ◽  
Enhanced Production

Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings,Bacillussp. IND12 was selected for fibrinolytic enzyme production.Bacillussp. IND12 effectively used cow dung for its growth and enzyme production (687±6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO4were the vital parameters with statistical significance (p<0.001). Three factors (moisture, sucrose, and MgSO4) were further studied through experiments of central composite rotational design and response surface methodology. Enzyme production of optimized medium showed4143±12.31 U/g material, which was more than fourfold the initial enzyme production (978±36.4 U/g). The analysis of variance showed that the developed response surface model was highly significant (p<0.001). The fibrinolytic enzyme digested goat blood clot (100%), chicken skin (83±3.6%), egg white (100%), and bovine serum albumin (29±4.9%).

scholarly journals Lovastatin Production from Cunninghamella blakesleeana under Solid State Fermentation

2021 ◽  
Author(s):  
Janani Balraj ◽  
Thandeeswaran Murugesan ◽  
Vidhya Kalieswaran ◽  
Karunyadevi Jairaman ◽  
Devippriya Esakkimuthu ◽  
...  
Keyword(s):  
Solid State ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
Experimental Studies ◽  
Nitrogen Sources ◽  
Medium Composition ◽  
Critical Parameters ◽  
Physical Parameters ◽  
Cunninghamella Blakesleeana

Abstract Our earlier paper had established the fact that new soil fungi known as Cunninghamella blakesleeana is potent enough to produce lovastatin significantly. At present, there are no reports on the media optimization for the lovastatin production. Hence, the objective is to optimize the fermentation conditions for lovastatin production by Cunninghamella blakesleeana under Solid State fermentation (SSF) condition through screening the critical factors by one factor at a time and then, optimize the factors selected from screening using statistical approaches. SSF was carried using the pure culture of Cunninghamella blakesleeana KP780148.1 with wheat bran as substrate. Initial screening was performed for physical parameters, carbon sources and nitrogen sources and then optimized the selected parameters through PBD and BBD. Screening result indicated the optimum values of the analysed parameter for the maximal production of lovastatin by Cunninghamella blakesleeana were selected. Out of the nine factors MgSO4, (NH4)2SO4, pH and Incubation period were found to influence the lovastatin production significantly after PBD. The optimal levels of these variables and the effect of their mutual interactions on lovastatin production were determined using BBD surface design. The optimum medium composition was found to be MgSO4(0.2 g/L), (NH4)2 SO4 (12.5 g/L), pH (6) and Incubation period (7 days). Experimental studies showed a yield of 7.39 mg/g at the above optimized conditions which were observed to be very nearby to the predicted value and hence the model was successfully validated. Hence, this is the first report on the optimization of critical parameters for lovastatin production by Cunninghamella blakesleeana.

scholarly journals A Review on Production of Polygalacturonase Using Various Organisms and Its Applications

2019 ◽  
pp. 1-12
Author(s):  
A. Radha ◽  
R. Sneha ◽  
R. Kiruthiga ◽  
P. Priyadharshini ◽  
N. Prabhu
Keyword(s):  
Solid State ◽  
Physicochemical Properties ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Full Range ◽  
Carbon Sources ◽  
Oil Extraction ◽  
Hydrolytic Cleavage ◽  
Biotechnological Application ◽  
Pectinolytic Enzyme

Polygalacturonase is a pectinolytic enzyme that catalyses the hydrolytic cleavage of the polygalacturonic linkage chain. An enzyme is a polygalacturonase is expressed in fruits. The polygalacturonase produced from various organisms isolated from various fruits. The solid-state fermentation was used in the production of polygalacturonase. The production of PG was found at various incubation period and pH and temperature are using fruits as best nitrogen and carbon sources. Although they have other parts of the genome they are active in the fruit. Peak expression requires a full range of promoter and saturation lines. Based on the physicochemical properties of the purified enzymes, this enzyme possesses great potential for industrial and biotechnological application such as oil extraction, fruit clarifications.

scholarly journals A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Moumita Karmakar ◽  
Rina Rani Ray
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Solid State Fermentation ◽  
Substrate Concentration ◽  
Water Hyacinth ◽  
Enzyme Production ◽  
Rhizopus Oryzae ◽  
Aquatic Weed ◽  
The Impact ◽  
Central Composite

The production cost of β-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (SSF). In the present study, the effect of different production conditions on the yield of β-glucosidase and endoglucanase by Rhizopus oryzae MTCC 9642 from water hyacinth was investigated systematically using response surface methodology. A Central composite experimental design was applied to optimize the impact of three variables, namely, substrate concentration, pH, and temperature, on enzyme production. The optimal level of each parameter for maximum enzyme production by the fungus was determined. Highest activity of endoglucanase of 495 U/mL was achieved at a substrate concentration of 1.23%, pH 7.29, and temperature 29.93°C whereas maximum β-glucosidase activity of 137.32 U/ml was achieved at a substrate concentration of 1.25%, pH 6.66, and temperature 32.09°C. There was a direct correlation between the levels of enzymatic activities and the substrate concentration of water hyacinth as carbon source.

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