scholarly journals Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

2010 ◽  
Vol 7 (3) ◽  
pp. 1159-1165
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Cold Water ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Diffusion Method ◽  
Resistant Bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds

scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE

2019 ◽  
pp. 113-115
Author(s):  
PURIT PATTANAPANIT ◽  
SUNISA MITHONGLANG ◽  
SUNITA MITHONGLANG ◽  
SURACHAI TECHAOEI
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Aromatic Plants ◽  
Volatile Oils

Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargoniumroseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria wereobtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcusaureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusionassay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayedby disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oilwith MIC and MBC of 1.25%.Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation providessupport to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.

scholarly journals Giloy (Tinospora Cordifolia): In Vitro Evaluation of Antibacterial Properties

2021 ◽  
Vol 07 (06) ◽  
Author(s):  
Tulsa Devi ◽  
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Water Extract ◽  
Hot Water ◽  
Tinospora Cordifolia ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Hot Water Extract ◽  
Agar Well Diffusion Method

Antibiotic resistance has become a global concern and hence, the search for other source of antimicrobials initiated to find a way to control infections in future. The main objective of this paper is to screen Giloy (Tinospora cordifolia) for its antibacterial activity. The stem of Tinospora cordifolia is used to prepare extract for determining it’s in vitro antibacterial activity as per the agar well diffusion method. In the agar well diffusion method 100μl of 24 hr broth culture of bacteria was aseptically and evenly swabbed on Mueller Hinton agar plates. Wells of about 8 mm diameter were aseptically cut using sterile cork-borer. 100 μl of plant extracts of different concentrations were then placed into the separate wells. The plates were incubated at 37 oC for 24hr. Antimicrobial activity of the giloy was determined by measuring the diameter of zone of inhibition. The methanolic extract of Tinospora cordifolia showed 13, 11, 9 and 5 mm zone of inhibition in S. aureus cultures by using 100, 75, 50 and 25 mg/ml concentration, respectively while hot water extract of Tinospora cordifolia showed 14, 12, 10 and 8 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25 mg/ml concentration, respectively and the cold extract of Tinospora cordifolia showed 10, 8, 5 and 0 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25mg/ml concentration, respectively. The methanolic extract of Tinospora cordifolia indicated 12, 10, 6 and 4 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively and the hot water extract of Tinospora cordifolia showed 16, 14, 12 and 10 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25mg/ml concentration, respectively. The cold water extract of Tinospora cordifolia showed 13, 10, 8, and 5 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively. It has been observed that Tinospora cordifolia showed very promising results as indicated by the zone of inhibition of bacterial culture through agar well diffusion method that varies from few mm to few cm. This study indicates the in-vitro antibacterial effect of Giloy which needs further validation through in-vivo studies.

Antimicrobial activity of Syzygium caryophyllatum L.

2021 ◽  
pp. 4275-4276
Author(s):  
Rohit Raj ◽  
Chandrashekar. K. S ◽  
Vasudev Pai
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Small Tree ◽  
Good Antibacterial Activity ◽  
Tropical Area ◽  
The Family ◽  
Agar Diffusion Method ◽  
India And China

Syzygium caryophyllatum L. is a small tree or large shrub grow widely mainly in the tropical area. It is native to India and China. S. caryophyllatum L. belonging to the family Myrtaceae is taken for screening antimicrobial activity. Ethanolic extract of the leaves of Syzygium caryophyllatum was screened for antibacterial activity using Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Activity of ethanolic extract was good when compared to Ampicillin. The antimicrobial activity was determined by Agar diffusion method and also MIC technique. From the result it was found that ethanolic extract of the leaves of Syzygium caryophyllatum exhibited good antibacterial activity against these gram +ve and gram –ve microorganisms.

scholarly journals Antibacterial Activity of Endostemon tereticaulis (Poir.) M. Ashby Essential Oil and Ethanolic Extract Against Resistant Pathogenic Bacteria

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095325
Author(s):  
Bala Namata Abba ◽  
Abderrahmane Romane ◽  
Amadou Tidjani Ilagouma
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Bactericidal Concentration

Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), ( E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase- Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190 , Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.

scholarly journals Antimicrobial Activity of two Mentha Species Essential Oil and its Dependence on Different Origin and Chemical Diversity

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Mária Pl'uchtová ◽  
Teresa Gervasi ◽  
Qada Benameur ◽  
Vito Pellizzeri ◽  
Daniela Grul'ová ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Chemical Diversity ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Growth Inhibition Zone

Genus Mentha presents group of plants which are the most studied in family Lamiaceae. Aboveground parts are used for different purposes in pharmacy, food industry or confectionery. Most important is natural product extracted from leaves - essential oil (EO). The aim of presented experiment was to demonstrate different chemotype and compare antibacterial activity of two Mentha species EO. Plant samples were obtained from various environments – from Slovakia and from Italy. Dominant compounds were determined by GC/MS. The results showed high amount of menthol and menthone in tested Slovak peppermint EO. On the other hand, carvone and 1,8-cineole were determinate as dominant compounds in Italian spearmint EO. The antimicrobial activity of the EO was investigated by disc diffusion and broth micro dilution methods. EO was evaluated for their antibacterial activity against 7 microorganisms: Enterobacter cloacae, Salmonella spp., Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes. The determination results of antibacterial activity by agar disk diffusion method ranged from 7 to 14 mm of the growth inhibition zone. MIC of tested mint EO varied from 0.625 to 2.5 μg/mL. In addition, both EO showed relatively the same antibacterial activity against the selected Gram-negative bacteria. However, there is a variation in the antibacterial activity against Gram-positive bacteria.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

Evaluation of Antibacterial Activity of Combined Plant Extract of Pyracantha crenulata and Zanthoxylum armatum

2020 ◽  
Vol 2 (29) ◽  
pp. 32-36
Author(s):  
Semwal Amit Negi Sweta
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Disk Diffusion Method ◽  
Zanthoxylum Armatum ◽  
Plant Extraction

Abstract-Medicinal plants represent an essential source of drugs and have played an important role in healthcaresystem.PyracanthacrenulataandZanthoxylum armatumhave been used as traditional medicine. The main aim of the study was to find the antibacterial activity of the selected plants against bacterialspecies:E.coliandPseudomonasaeruginosa. The solvents used for plant extraction were hexane, chloroform, ethanol and aqueous. The in vitro antimicrobial activity was performed by Agar disk diffusion method. The hexane and aqueous extracts showed moderate activity whereas theethanolicextractsshowedasignificantantibacterial activity. In the study Tetracycline was used as standard. The combined ethanolic extract of both the selected plant showed the synergistic effect on the bacterial strain tested. This leads to the conclusion that the combined effect can have possible application in the development of products as antimicrobial.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

scholarly journals Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Western Ghats ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

scholarly journals Antibacterial Activity of Some Herbal Extracts for the Treatment of Typhoid Fever Sold in Okitipupa Town, Ondo State, Nigeria

2021 ◽  
Vol 6 (1) ◽  
pp. 86-90
Author(s):  
I. A. Adeyemo ◽  
◽  
C. O. Omolade
Keyword(s):  
Antibacterial Activity ◽  
Typhoid Fever ◽  
Test Organism ◽  
Water Extract ◽  
Salmonella Typhi ◽  
Hot Water ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Herbal Mixture ◽  
Plant Parts

The study investigated and compared the antimicrobial potency of aqueous (cold and hot) and ethanolic extracts of six plant parts used as herbal mixture against clinical isolate of Salmonella typhi in Okitipupa town. Extracts from six plant parts used as herbal mixture were tested for antibacterial activity against Salmonella typhi isolated from patients suffering from typhoid fever. The antibacterial activities of each of the extracts (ethanolic, cold and hot waters) were determined using agar diffusion method and the activities produced zone of inhibition ranging from 20mm-34mm. Ethanolic extract was found to be more potent being capable of exerting significant inhibitory activity on the test organism investigated with an inhibition zone of 34mm followed by the cold-water extract (28mm) while hot water extract gave the least (20mm). The phytochemical analysis showed the presence of saponins, tannins, flavonoids, phenols, glucosinolates and triterpenes in the extracts but total absence of proteins. The study confirms the medicinal value of herbal mixtures and justifies the claim of its local use in health remedies for typhoid fever caused by Salmonella typhi. There is the need therefore to develop effective combination of antimicrobial agents in purified form from higher plants and their parts for clinical trials. Keywords: Herbal mixture, antibacterial activity, typhoid fever, phytochemical analysis.

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