Predictive Values of the Selected Inflammatory Index in the Progression of Colon Cancer Patients

Abstract Background: Ample evidence has revealed that the lymphocyte-to-monocyte ratio (LMR), albumin-to-globulin ratio (AGR) and mean platelet volume (MPV) are cancer-related inflammatory markers. The present study aimed to assess a better diagnostic marker for the progression of colon cancer. Methods: This retrospective study enrolled 251 patients with colon cancer, 171 patients with benign colon diseases, and 187 healthy control subjects from January 2012 to September 2020. The receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to determine the diagnostic values of the selected inflammatory index.Results: The levels of LMR, AGR and MPV were decreased in the colon cancer group compared with the healthy control and benign colon disease groups. The LMR, AGR and MPV were all correlated with tumor size. Moreover, LMR and AGR was associated with lymph node metastasis and clinical stage, AGR was related to distant metastasis. Both the LMR (p = 0.030) and AGR (p = 0.005) were negatively correlated with the concentration of carcinoembryonic antigen (CEA). The AUC value of MPV combined with CEA had a good diagnostic ability for distinguishing controls from colon cancer cases (AUC = 0.950) and patients with benign colon diseases (AUC = 0.886). Meanwhile, the combination of LMR or AGR with CEA could enhance the diagnostic efficacy (AUC; 0.746 for LMR + CEA, 0.737 for AGR + CEA) of detecting colon cancer from benign colon diseases. Conclusions: CEA combined with the selected inflammatory index may be used as better blood-based biomarkers in the progression of colon cancer patients.

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Diagnostic value of the lymphocyte-to-monocyte and albumin-to-globulin ratios in patients with colon cancer

10.21203/rs.3.rs-18853/v1 ◽

2020 ◽

Author(s):

Li Huang ◽

Zhuning Mo ◽

Zuojian Hu ◽

Linyan Zhang ◽

Shanzi Qin ◽

...

Keyword(s):

Colon Cancer ◽

Clinical Stage ◽

Diagnostic Value ◽

Chi Square ◽

Diagnostic Efficacy ◽

Ample Evidence ◽

Lymphocyte To Monocyte Ratio ◽

Cancer Group ◽

Healthy Control ◽

Colon Diseases

Abstract Background: Ample evidence has revealed that the lymphocyte-to-monocyte ratio (LMR) and albumin-to-globulin ratio (AGR) are cancer-related inflammatory markers. The present study aimed to assess whether LMR and AGR, alone or in combination with carcinoembryonic antigen (CEA), was a useful diagnostic marker for colon cancer.Methods: This retrospective study enrolled 251 patients with colon cancer, 171 patients with benign colon diseases, and 187 healthy control subjects from January 2012 to September 2019. Mann-Whitney U test or Chi-square test were used to analyze differences between groups in laboratory parameters and clinicopathological features. The diagnostic value of LMR and AGR combined or not with CEA in colon cancer patients was determined via a receiver operating characteristic (ROC) curve.Results: The levels of LMR and AGR were decreased in the colon cancer group compared with the healthy control and benign colon disease groups. The LMR and AGR were correlated with lymph node metastasis, tumor size, and clinical stage. Moreover, AGR was associated with distant metastasis. Both the LMR (r = −0.137, p = 0.030) and AGR (r = −0.178, p = 0.005) were negatively correlated with the concentration of CEA. LMR or AGR combined with CEA could enhance sensitivity (75.30% for LMR + CEA, 58.57% for AGR + CEA) and generate larger area under curve (AUC; 0.75 for LMR + CEA, 0.74 for AGR + CEA) compared with the LMR (p < 0.001), AGR (p < 0.001), or CEA (p < 0.001) alone.Conclusion: The combination of LMR or AGR with CEA may enhance the sensitivity and diagnostic efficacy of detecting colon cancer from benign colon diseases.

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Value and Diagnostic Efficacy of Fetal Morphology Assessment Using Ultrasound in A Poor-Resource Setting

Diagnostics ◽

10.3390/diagnostics9030109 ◽

2019 ◽

Vol 9 (3) ◽

pp. 109 ◽

Cited By ~ 1

Author(s):

Ukweh ◽

Ugbem ◽

Okeke ◽

Ekpo

Keyword(s):

Predictive Value ◽

Performance Metrics ◽

Area Under The Curve ◽

Youden Index ◽

Likelihood Ratios ◽

Diagnostic Efficacy ◽

Predictive Values ◽

Resource Setting ◽

Post Test ◽

Sensitivity Specificity

Background: Ultrasound is operator-dependent, and its value and efficacy in fetal morphology assessment in a low-resource setting is poorly understood. We assessed the value and efficacy of fetal morphology ultrasound assessment in a Nigerian setting. Materials and Methods: We surveyed fetal morphology ultrasound performed across five facilities and followed-up each fetus to ascertain the outcome. Fetuses were surveyed in the second trimester (18th–22nd weeks) using the International Society of Ultrasound in Obstetrics and Gynecology (ISUOG) guideline. Clinical and surgical reports were used as references to assess the diagnostic efficacy of ultrasound in livebirths, and autopsy reports to confirm anomalies in terminated pregnancies, spontaneous abortions, intrauterine fetal deaths, and still births. We calculated sensitivity, specificity, positive and negative predictive values, Area under the curve (AUC), Youden index, likelihood ratios, and post-test probabilities. Results: In total, 6520 fetuses of women aged 15–46 years (mean = 31.7 years) were surveyed. The overall sensitivity, specificity, and AUC were 77.1 (95% CI: 68–84.6), 99.5 (95% CI: 99.3–99.7), and 88.3 (95% CI: 83.7–92.2), respectively. Other performance metrics were: positive predictive value, 72.4 (95% CI: 64.7–79.0), negative predictive value, 99.6 (95% CI: 99.5–99.7), and Youden index (77.1%). Abnormality prevalence was 1.67% (95% CI: 1.37–2.01), and the positive and negative likelihood ratios were 254 (95% CI: 107.7–221.4) and 0.23 (95% CI: 0.16–0.33), respectively. The post-test probability for positive test was 72% (95% CI: 65–79). Conclusion: Fetal morphology assessment is valuable in a poor economics setting, however, the variation in the diagnostic efficacy across facilities and the limitations associated with the detection of circulatory system anomalies need to be addressed.

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Determination of Serum Angiopioetin-2 levels in Breast cancer patients at Damascus University Hospitals

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00843 ◽

2021 ◽

pp. 4856-4858

Author(s):

Majd Haidar ◽

Jumana Al Saleh

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Clinical Stage ◽

Tumor Grade ◽

Serum Levels ◽

Breast Cancer Patients ◽

University Hospitals ◽

Healthy Control ◽

Elisa Technique ◽

Angiopoietin 2

Background: Breast cancer has increased universally. Angiogenesis is an essential step for cancer growth, progression and metastasis. Recent studies have focused on Angiopoietin-2 as a promising biomarker in prospecting the prognosis of the cancer. Objective: Determine the relation between serum concentrations of Angiopoietin-2 and clinicopathological data in breast cancer patients. Methodology: This study is prospective, cohort type. It includes 30 women with breast cancer, who met the study criteria. The serum Ang-2 value was calibrated using ELISA technique. The data was processed using SPSS program. Results: Ang-2 serum levels were statistically significant associated with ER status (P = 0.036). However, no signifi¬cant correlation was observed between serum Ang-2 level and the other clinicopathologic param¬eters tested, including Histological type (P = 0.417), Clinical grade G (P = 0.91), Clinical stage T (P = 0.913), Clinical stage N (P = 0.086), PR status (P = 0.467), and HER-2 status (P = 0.791). Serum levels of Ang-2 for breast cancer patients was significantly higher than healthy control (20411.33± 6283.19 vs 1731.70± 368.35 pg/ml, P< 0.000). our data indicates that serum levels of Ang-2 has no correlation between the tumor grade and stage in breast cancer patients.

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Activity of cancer procoagulant (CP) in serum of patients with cancer of lung, breast, oesophagus and colorectum.

Acta Biochimica Polonica ◽

10.18388/abp.1997_4446 ◽

1997 ◽

Vol 44 (1) ◽

pp. 109-112 ◽

Cited By ~ 4

Author(s):

M Rucińska ◽

M Furman ◽

Z Skrzydlewski ◽

E Zaremba

Keyword(s):

Blood Serum ◽

Cancer Patients ◽

Malignant Tumor ◽

Clinical Stage ◽

Healthy People ◽

Colorectal Cancers ◽

Patients With Cancer ◽

Healthy Control ◽

Cancer Procoagulant ◽

Radical Removal

Activity of cancer procoagulant (CP) was studied in blood serum of 90 patients with cancer of lung, breast, oesophagus and colorectum, and of 15 healthy people. The activity of CP was determined by the coagulation method. Sera of patients with cancer showed higher mean activity of CP than sera of healthy control. Of the 90 cancer patients 78 were identified correctly by this test as having cancer (sensitivity 85%). In the case of lung and colorectal cancers the higher CP activity was observed the more advanced was the clinical stage of cancer, and the test was positive in 100%. After radical removal of malignant tumor of lung, decreased CP activity was found.

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Unfavorable clinical implications of peripheral blood CD44+ and CD54+ lymphocytes in patients with lung cancer undergoing chemotherapy

The International Journal of Biological Markers ◽

10.5301/ijbm.5000309 ◽

2017 ◽

Vol 33 (2) ◽

pp. 208-214 ◽

Cited By ~ 1

Author(s):

Zhuanbo Luo ◽

Yun Wang ◽

Yanru Lou ◽

Chao Cao ◽

Richard Hubbard ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Patients ◽

Peripheral Blood ◽

Lymphocyte Subsets ◽

Clinical Stage ◽

Unmet Need ◽

Control Group ◽

Lung Cancer Patients ◽

Healthy Control

Background: There is an unmet need for identification of additional prognostic markers for lung cancer. The aim of this study was to identify novel clinical and immunological predictors of prognosis in lung cancer patients. Methods: Lymphocyte subsets CD3+, CD4+, CD8+, CD4+/8+, CD25+, CD69+, CD44+ and CD54+ were quantified in peripheral blood using flow cytometry, for 203 newly diagnosed lung cancer patients and 120 healthy controls. Results: The levels of CD3+, CD4+, CD8+, CD4+/CD8+ and CD69+ lymphocytes were significantly lower in patients with lung cancer compared with the healthy control group, while CD54+ and CD44+ lymphocytes were significantly higher. In stage III/IV patients with lymph node metastasis or distant metastasis, the levels of CD44+ and CD54+ lymphocytes were significantly increased compared with patients with stage I/II disease (p<0.05). The levels of CD44+ and CD54+ lymphocytes markedly reduced after chemotherapy, and follow-up analysis indicated that patients found without increase of CD44+ and CD54+ lymphocytes after chemotherapy had survival advantages. Independent predictors of survival in lung cancer patients included clinical stage (hazard ratio [HR] = 2.791; 95% confidence interval [95% CI], 1.42-3.54, p<0.001), CD44+ lymphocytes (HR = 1.282; 95% CI, 1.02-1.49, p = 0.002) and CD54+ lymphocytes (HR = 1.475; 95% CI, 1.22-1.73, p = 0.003). Elevated levels of CD44+ and CD54+ lymphocytes correlated with poor prognosis in lung cancer patients. Conclusions: Peripheral blood lymphocyte subsets in patients with lung cancer are different from those in healthy people, and circulating CD44+ and CD54+ lymphocytes seem to be a promising criterion to predict survival in lung cancer patients undergoing chemotherapy.

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Discovery of N-glycan Biomarkers for the Canine Osteoarthritis

Life ◽

10.3390/life10090199 ◽

2020 ◽

Vol 10 (9) ◽

pp. 199

Author(s):

Hyunjun Lee ◽

Ahyun Lee ◽

Nari Seo ◽

Jiwon Oh ◽

Oh-Kyeong Kweon ◽

...

Keyword(s):

Solid Phase ◽

Area Under The Curve ◽

Protein Glycosylation ◽

Post Translational Modification ◽

Clinical Tool ◽

Protein Activity ◽

Diagnostic Efficacy ◽

Diagnostic Biomarkers ◽

Healthy Control ◽

Canine Serum

Protein glycosylation is a post-translational modification that impacts on protein activity, stability, and interactions. It was sensitively altered by the cellular state and, therefore, is now used for a diagnostic or prognostic indicator of various human diseases such as cancer. To evaluate the clinical feasibility in the veterinary area, the N-glycan biomarkers were discovered from canine serum for the diagnosis of osteoarthritis (OA), which is one of the most common diseases of dogs. N-glycome was obtained from 20 μL of canine serum by the enzymatic cleavage followed by the purification and enrichment using solid-phase extraction. Independent compositions of 163 and 463 N-glycans were found from healthy control (n = 41) and osteoarthritis patients (n = 92), respectively. Initially, 31 of the potential biomarkers were screened by the p-values below 1.0 × 10−10 from ANOVA. Then, the area under the curve (AUC) and the intensity ratio between OA patient and healthy control (P/C ratio) were calculated. Considering the diagnostic efficacy, the AUC bigger than 0.9 and the P/C ratio larger than 3.0 were used to discover 16 N-glycans as diagnostic biomarkers. Particularly, five of the diagnostic biomarkers were AUC above 0.99 and three of N-glycans had AUC 1.0. The results suggest a clear possibility for N-glycan biomarkers to be used as a clinical tool in the veterinary medical area enabling to provide objective and non-invasive diagnostic information.

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