scholarly journals Integrated Control of Aedes Aegypti With Parasitic Nematodes, Biological Control Agents, Chemical Insecticides and Plant Essential Oil

2021 ◽  
Author(s):  
Jinfeng Xiong ◽  
Hui Zhang ◽  
Caixia Li ◽  
Rui Ma ◽  
Hui Ai ◽  
...  
Keyword(s):  
Biological Control ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
High Efficiency ◽  
Pcr Analysis ◽  
Control Group ◽  
Mosquito Vector ◽  
Biological Control Agents ◽  
Competitive Binding Assay ◽  
Density Control

Abstract Aedes aegypti can transmit dengue fever, yellow fever, Chikungunya fever, Zika virus disease and vector density control is the most effective way to prevent these infectious diseases. However, the extensive use of chemical pesticides has caused a series of problems, such as environmental pollution, killing non-target organisms and so on. In this study, a parasitic nematode, Romanomermis wuchangensis was used in the larviciding evaluation of Ae. aegypti, while the activity of four chemical insecticides and biological control agents were tested. Besides, Mentha haplocalyx essential oil was isolated and its olfactory physiological function with OBP1 protein of Ae. aegypti antenna was measured by the prokaryotic expression and fluorescence competitive binding assay. Compared with the control group, R. wuchangensis indicated high efficiency and environmental friendliness in the control of Ae. aegypti. After the second instar larvae were parasitized, the mortality of two treatment groups exceeded 75%. Compared to control group, the quantitative real-time PCR analysis results demonstrated that SOD, POD and CAT genes had obvious high expression levels in the nematodes parasitic groups. The antioxidant enzyme test results also exhibited obvious difference of SOD, CAT and POD during the nematode parasitic period. Besides, Bacillus thuringiensis (Bti) and chemical insecticide experimental results also showed great insecticidal efficacy against mosquito larvae. Five chemical components including Menthol, Pinene, Limonene, Isopulegol and Pulegone were identified from M. haplocalyx and exhibited great binding ability with AaegOBP1 protein. Present results illustrated that the integrated application of these various mosquito vector control methods in the future has broad prospects.

scholarly journals Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

2021 ◽  
Vol 15 (7) ◽  
pp. e0009587
Author(s):  
Ivana Sierra ◽  
Jose Manuel Latorre-Estivalis ◽  
Lucila Traverso ◽  
Paula V. Gonzalez ◽  
Ariel Aptekmann ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Vector Control ◽  
Gene Families ◽  
Control Group ◽  
Mosquito Vector ◽  
Rna Seq ◽  
Chemosensory Proteins ◽  
The Impact

Background Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

scholarly journals Blue Tansy Essential Oil: Chemical Composition, Repellent Activity Against Aedes aegypti and Attractant Activity for Ceratitis capitata

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199019 ◽  
Author(s):  
Iris Stappen ◽  
Juergen Wanner ◽  
Nurhayat Tabanca ◽  
Ulrich R. Bernier ◽  
Paul E. Kendra
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Ceratitis Capitata ◽  
Stationary Phases ◽  
Management Strategies ◽  
Fruit Fly ◽  
Effective Dosage ◽  
Mosquito Vector ◽  
Tea Tree ◽  
Potential Applications

Blue tansy essential oil (BTEO) ( Tanacetum annuum L.) was analyzed by GC-MS and GC-FID using two different capillary column stationary phases. Sabinene (14.0%), camphor (13.6%), myrcene (8.0%), β-pinene (7.7%), and chamazulene (6.9%) were the main components using an SE52 column (non-polar). On a polar CW20M phase column, sabinene (15.1%), camphor (14.4%), α-phellandrene (7.9%), β-pinene (7.7%), and myrcene (6.9%) were the most abundant compounds. To assess the oil for potential applications in integrated pest management strategies, behavioral bioassays were conducted to test for repellency against yellow fever mosquito Aedes aegypti, and for attractant activity for Mediterranean fruit fly Ceratitis capitata. Results showed that BTEO was not effective in repelling Ae. aegypti (minimum effective dosage [MED]: 0.625 ± 0.109 mg/cm2 compared with the standard insect repellent DEET (N,N-diethyl-3-methylbenzamide). In assays with male C. capitata, BTEO displayed mild attraction compared with two positive controls (essential oils from tea tree Melaleuca alternifolia and African ginger bush Tetradenia riparia). Additional studies are needed to identify the specific attractant chemicals in BTEO and to determine if they confer a synergistic effect when combined with other known attractants for C. capitata. To the best of our knowledge, this study represents the first investigation of BTEO for repellency against the mosquito vector Ae. aegypti and for attractancy to C. capitata, a major agricultural pest worldwide.

scholarly journals AKTIVITAS EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) SEBAGAI LARVASIDA TERHADAP Aedes aegypti DI KECAMATAN KELAPA LIMA KOTA KUPANG

2020 ◽  
Vol 8 (1) ◽  
pp. 54-68
Author(s):  
Deswandi W. S. Berri ◽  
Julianty Almet ◽  
Diana Agustiani Wuri
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Research Method ◽  
Hemorrhagic Fever ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mosquito Vector ◽  
Curcuma Xanthorrhiza ◽  
Mosquito Vector Control

Dengue hemorrhagic fever (DHF) is a disease that is found in some tropical and subtropical regions. This disease is caused by dengue virus and is transmitted to humans through the bite of the Aedes aegypti mosquito. the solution taken in controlling DHF is to break the life cycle of the Aedes aegypti mosquito. Vector control is generally carried out using synthetic larvicides, namely abate / temefos, but the use of abate can cause residues, environmental pollution, poisoning and resistance of the eradicated vectors so that natural larvasides from plants are needed namely temulawak rhizome (Curcuma xanthorrhiza Roxb.) for vector control. The purpose of this study was to determine whether temulawak rhizome extract was effective or not in killing Aedes aegypti larvae. This research method includes larva collection, identification and maintenance of mosquitoes, determining sample size, making extracts and testing effectiveness. This study used a control and experiment group with 3 repetitions in the minutes to 15, 30, 45, 60 and 1440 (24 Hours). The control group was positive control using abate and negative control using aquades while the eksperiment group used extract of temulawak rhizome (Curcuma xanthorrhiza Roxb.) with concentrations of 0.6%, 0.8%, 1%, 1.2% and 1.5%. The results of this study indicate that the temulawak rhizome extract (Curcuma xanthorrhiza Roxb.) effective as larvicides because at the lowest concentration 0.6% can kill 100% Aedes aegypti larvae.

scholarly journals Impact of Cymbopogon flexuosus (Poaceae) essential oil and primary components on the eclosion and larval development of Aedes aegypti

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ruth Mariela Castillo-Morales ◽  
Sugey Ortiz Serrano ◽  
Adriana Lisseth Rodríguez Villamizar ◽  
Stelia Carolina Mendez-Sanchez ◽  
Jonny E. Duque
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
High Performance ◽  
Geranyl Acetate ◽  
Control Group ◽  
Head Width ◽  
Growth Inhibitors ◽  
Egg Hatching ◽  
Larval Length ◽  
Cymbopogon Flexuosus

AbstractThe current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti. We treated eggs with 6, 18, or 30 mg L−1 and larvae with 3 or 6 mg L−1 of EO and its major compounds (citral and geranyl acetate). Citral and geranyl acetate were evaluated at 18, 30, and 42 mg L−1 and compared with commercial growth inhibitors (diflubenzuron and methoprene). We measured larval head diameter, siphon length, and larval length. Finally, we examined concentrations of molt hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography coupled to mass spectrometry. All geranyl acetate concentrations decreased egg hatching, while EO altered molting among larval instars and between larvae and pupae, with an increase in the larval length (3 mg L−1: 6 ± 0.0 mm; 6 mg L−1: 6 ± 0.7 mm) and head width (3 mg L−1: 0.8 ± 0 mm; 6 mg L−1: 0.8 ± 0.0 mm) compared with the control group. We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, namely decreased hormone concentrations, an extended larval period, and death.

Toxic effect of essential oil and its compounds isolated from Sphaeranthus amaranthoides Burm. f. against dengue mosquito vector Aedes aegypti Linn.

2019 ◽  
Vol 160 ◽  
pp. 163-170 ◽  
Author(s):  
Annamalai Thanigaivel ◽  
Kanagaraj Muthu-Pandian Chanthini ◽  
Sengodan Karthi ◽  
Prabhakaran Vasantha-Srinivasan ◽  
Athirstam Ponsankar ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Toxic Effect ◽  
Mosquito Vector

Evaluation of entomopathogenic fungi as potential biological control agents of the dengue mosquito,Aedes aegypti(Diptera: Culicidae)

2011 ◽  
Vol 21 (9) ◽  
pp. 1027-1047 ◽  
Author(s):  
Jonathan M. Darbro ◽  
Robert I. Graham ◽  
Brian H. Kay ◽  
Peter A. Ryan ◽  
Matthew B. Thomas
Keyword(s):  
Biological Control ◽  
Aedes Aegypti ◽  
Entomopathogenic Fungi ◽  
Biological Control Agents

scholarly journals Development of a Confinable Gene-Drive System in the Human Disease Vector, Aedes aegypti

2019 ◽  
Author(s):  
Ming Li ◽  
Ting Yang ◽  
Nikolay P. Kandul ◽  
Michelle Bui ◽  
Stephanie Gamez ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
High Efficiency ◽  
New Technologies ◽  
Current Control ◽  
Pathogen Transmission ◽  
Mosquito Vector ◽  
Control Methods ◽  
Split Gene ◽  
Pathogen Effector ◽  
Gene Drives

AbstractAedes aegypti, the principal mosquito vector for many arboviruses that causes yellow fever, dengue, Zika, and chikungunya, increasingly infects millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has recently sparked significant enthusiasm for the genetic control of mosquito populations, however no such system has been developed in Ae. aegypti. To fill this void and demonstrate efficacy in Ae. aegypti, here we develop several CRISPR-based split-gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild Ae. aegypti populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of Ae. aegypti to combat local pathogen transmission.Significance StatementAe. aegypti is a globally distributed arbovirus vector spreading deadly pathogens to millions of people annually. Current control methods are inadequate and therefore new technologies need to be innovated and implemented. With the aim of providing new tools for controlling this pest, here we engineered and tested several split gene drives in this species. These drives functioned at very high efficiency and may provide a tool to fill the void in controlling this vector. Taken together, our results provide compelling path forward for the feasibility of future effector-linked split-drive technologies that can contribute to the safe, sustained control and potentially the elimination of pathogens transmitted by this species.

scholarly journals Pemanfaatan Jeruk Purut (Citrus Hystrix) Sebagai Biolarvasida

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Titik Lestari
Keyword(s):  
Essential Oil ◽  
Literature Review ◽  
Aedes Aegypti ◽  
Research Methods ◽  
Chemical Compound ◽  
Mosquito Vector ◽  
The Third ◽  
Chemical Content ◽  
Lethal Time ◽  
Third Instar Larvae

Absctract: Citrus hystrix, Biolarvicides. Insectisides and pestisides were used as larvicides to control mosquito vector Aedes aegypti particularly. Some research suggested there had been a larvae resistance to pesticides. Biolarvicides with basic ingredients plant was another alternative that can be used. Lime (Citrus hystrix) was one of plants that often used as biolarvicides, both lits fruit and leaves. This paper aimed to know about chemical compound in lime and its potential as biolarvicidal. Research methods was literature review from several research journals. The results showed that the presence of coumpounds were essential oil, flavonoids, saponins, terpeesn, limonoids, citronellal, linalool, citronellol, acetate citronelil, kariofilin and geraniol. LC50 of lime leaves as biolarvicides againts the third instar larvae of Aedes aegypti was 279.882 ppm, biolarvicides oxicity values (LC90) was 4000 ppm with the lethal time (LT90) was 12.67 hours. Based on results of this study concluded that the presence of the chemical content of Citrus hystrix plant potential as biolarvicides.

scholarly journals Impact of Cymbopogon Flexuosus (Poaceae) Essential Oil and Primary Components on the Eclosion and Larval Development of Aedes Aegypti

2021 ◽  
Author(s):  
Ruth Mariela Castillo-Morales ◽  
Sugey Ortiz Serrano ◽  
Adriana Lisseth Rodríguez Villamizar ◽  
Stelia Carolina Méndez-Sánchez ◽  
Jonny Edward Duque
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
High Performance ◽  
Geranyl Acetate ◽  
Control Group ◽  
Head Width ◽  
Growth Inhibitors ◽  
Egg Hatching ◽  
Cymbopogon Flexuosus ◽  
Moult Hormone

Abstract The current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti. We treated eggs with 6, 18, and 30 mg.L-1 and larvae with 3 and 6 mg.L-1 EO concentrations. Citral and geranyl acetate were evaluated at 18, 30, and 42 mg.L-1 and compared to the commercial growth inhibitors (diflubenzuron and methoprene). We measured larval head diameter, siphon length, and body length. Finally, we examined concentrations of moult hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography coupled to mass spectrometry. The EO decreased egg hatching at all concentrations and altered molting among larval instars and between larvae and pupae, with an increase in the length (3 mg.L-1: 6 ± 0.0 mm; 6 mg.L-1: 6 ± 0.7 mm) and head width (3 mg.L-1: 0.8 ± 0 mm; 6 mg.L-1: 0.8 ± 0.0 mm) compared with the control group. We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, decreasing hormone concentration, extending the larval period, and death.

scholarly journals Impact of Cymbopogon flexuosus (Poaceae) essential oil and primary components on the eclosion and larval development of Aedes aegypti (Diptera: Culicidae)

2021 ◽  
Author(s):  
Ruth Mariela Castillo-Morales ◽  
Sugey Ortiz Serrano ◽  
Adriana Lisseth Rodríguez Villamizar ◽  
Stelia Carolina Mendez-Sanchez ◽  
Jonny E. Duque
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
High Performance ◽  
Morphological Analysis ◽  
Geranyl Acetate ◽  
Control Group ◽  
Head Width ◽  
Egg Hatching ◽  
Cymbopogon Flexuosus ◽  
Eggs And Larvae

AbstractThe current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti (L.) eggs and larvae. To demonstrate the ovicidal activity of EO, we treated embryonated eggs with 6, 18, and 30 mg.L-1 and larvae with 3 and 6 mg.L-1 EO concentrations. Citral and geranyl acetate were evaluated at 18, 30, and 42 mg.L-1 and compared with commercial growth inhibitors (diflubenzuron and methoprene) at 3 and 6 mg.L-1 concentrations. For each treatment, we measured larval head diameter, siphon length, and body length. To complement the morphological analysis, we examined concentrations of moult hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography (HPLC) coupled to mass spectrometry. The EO decreased egg hatching at all concentrations: 6 mg.L-1 in 45.3%; 18 mg.L-1 in 23.3%, and 30 mg.L-1 in 34.6%. EO also altered molting among larval instars and between larvae and pupae, with an increase in the length (3 mg.L-1: 6 ± 0.0 mm; 6 mg.L-1: 6 ± 0.7 mm) and head width (3 mg.L-1: 0.8 ± 0 mm; 6 mg.L-1: 0.8 ± 0.0 mm) compared with the control group (length: 5.3 ± 0 mm; head width: 0.7 ± 0.0 mm). We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, decreasing hormone concentration, extending the larval period, and death.

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