Cultured and Uncultured Microbial Community Associated With Biogas Production

2021 ◽  
Author(s):  
Júlia Ronzella Ottoni ◽  
Suzan Prado Fernandes Bernal ◽  
Tiago Joelzer Marteres ◽  
Franciele Natividade Luiz ◽  
Viviane Piccin dos Santos ◽  
...  
Keyword(s):  
Renewable Energy ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Biogas Production ◽  
Renewable Energy Sources ◽  
Starter Culture ◽  
Sequencing Analysis ◽  
Industrial Plants ◽  
Comprehensive Information ◽  
Culture Independent

Abstract The search for sustainable development has led countries around the world to seek the improvement of technologies that use renewable energy sources. One of the alternatives in the production of renewable energy comes from the use of waste including urban solids, animal excrement from livestock and biomass residues from agro-industrial plants. These materials may be used in the production of biogas, making its production highly sustainable and environmentally friendly, in addition to reducing public expenses for the treatment of those wastes. The present study evaluated the cultivated and uncultivated microbial community from a substrate (starter) used as an adapter for biogas production in anaerobic digestion processes. 16S rDNA metabarcoding revealed domain of bacteria belonging to the phyla Firmicutes, Bacteroidota, Chloroflexi and Synergistota. The methanogenic group was represented by the phyla Halobacterota and Euryarchaeota. Through 16S rRNA sequencing analysis of isolates recovered from the starter culture, the genera Rhodococcus, Vagococcus, Lysinibacillus, Niallia, Priestia, Robertmurraya, Luteimonas and Proteiniclasticum were recovered, groups that were not observed in the metabarcoding data. The groups mentioned are involved in the metabolism pathways of sugars and other compounds derived from lignocellulosic material, as well as in anaerobic methane production processes. The results demonstrate that culture-dependent approaches, such as isolation and sequencing of isolates, as well as culture-independent studies, such as the Metabarcoding approach, are complementary methodologies that, when integrated, provide robust and comprehensive information about the microbial communities involved in various processes, including the production of biogas in anaerobic digestion processes.

scholarly journals Anaerobic Digestion of Biomass for production of Biogas: Progress and Advantages

2020 ◽  
Vol 6 (5) ◽  
pp. 108-111
Author(s):  
Kumar Gaurav
Keyword(s):  
Renewable Energy ◽  
Anaerobic Digestion ◽  
Fossil Fuels ◽  
Biogas Production ◽  
Energy Requirement ◽  
Renewable Energy Sources ◽  
Agricultural Waste ◽  
Energy Generation ◽  
Primary Energy ◽  
Energy Sources

A major share of world’s primary energy requirement is dependent on fossil fuels which is not only a non renewable source of energy and on the verge of extinction but also associated with serious environmental concerns. To combat these issues, alternative renewable energy sources are required. Certain examples of renewable energy sources are solar energy, wind energy, hydro and thermal energy, biofuels etc. Biomass is one such alternative which is freely and abundantly available. It is mainly the agricultural waste and vegetable waste which are perishable and create a lot of nuisance. Tapping this biomass for energy production will be beneficial in two ways; it will be an excellent source of energy generation and it will also help in waste management for environment protection. Energy generation from Biomass can take place either chemically or thermo-chemically. In the present paper advantages of anaerobic digestion of biomass are discussed for biogas production.

Anaerobic digestion: Technology for biogas production as a source of renewable energy—A review

2020 ◽  
pp. 0958305X2092311 ◽  
Author(s):  
KeChrist Obileke ◽  
Nwabunwanne Nwokolo ◽  
Golden Makaka ◽  
Patrick Mukumba ◽  
Helen Onyeaka
Keyword(s):  
Renewable Energy ◽  
Anaerobic Digestion ◽  
Biogas Production ◽  
Renewable Energy Sources ◽  
Temperature Stability ◽  
Clean Energy ◽  
Appropriate Technology ◽  
Biogas Energy ◽  
Metabolic Activities ◽  
Insight Into

The authors reviewed the future prospects and previous studies on anaerobic digestion technology for biogas production and highlight the solutions to problems relating to construction and maintenance of biogas digesters, which can now be accessed in a single paper. It is the aim of the review to provide insight into the use, process and application of anaerobic digestion as an appropriate technology for biogas production from peer reviewed literature. Recent studies have shown that the microbial communities and metabolic pathways involves in anaerobic digestion are influenced by temperature. Their metabolic activities increase significantly with increase in temperature. Therefore, the findings of the review reveal that temperature is a major parameter for biogas production due to its influence on metabolic activities involved in anaerobic digestion. Hence, there is the need for insulation as well as external heating to maintain temperature stability and to avoid temperature fluctuations. More also, the anaerobic digestion technology for production of biogas is a viable option that can supplement as well as reduce the usage of non-renewable energy sources such as fossil fuel. The detailed information addressed in this study would increase biogas energy mix as well as mitigating climate change. Therefore, the study recommends the use of biogas as a clean energy for the purpose of power generation, cooking and heating.

scholarly journals Regional Diversification of Potential, Production and Efficiency of Use of Biogas and Biomass in Poland

Energies ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 742
Author(s):  
Grzegorz Ślusarz ◽  
Barbara Gołębiewska ◽  
Marek Cierpiał-Wolan ◽  
Jarosław Gołębiewski ◽  
Dariusz Twaróg ◽  
...  
Keyword(s):  
Renewable Energy ◽  
Biomass Production ◽  
Biogas Production ◽  
Renewable Energy Sources ◽  
Energy Sources ◽  
Efficiency Frontier ◽  
The European Union ◽  
Production Potential ◽  
Solid Biomass ◽  
High Share

Energy obtained from renewable sources is an important element of the sustainable development strategy of the European Union and its member states. The aim of this research is, therefore, to assess the potential and use of renewable energy sources and their effectiveness from the regional perspective in Poland. The research covered the years 2012 and 2018. The diversification of production and potential of renewable energy sources was defined on the basis of biogas and biomass. Calculations made using the data envelopment analysis (DEA) method showed that, in 2012, only three voivodeships achieved the highest efficiency in terms of the use of biogas and biomass resources; in 2018, this number increased to four. Comparing the effective units in 2012 and 2018, it can be seen that their efficiency frontier moved upwards by 56% in terms of biogas and 21% in terms of to biomass. Despite a large relative increase in the production of heat from biogas by 99% compared to the production of heat from biomass by 38%, the efficiency frontier for biogas did not change considerably. It was found that the resources of solid biomass are used far more intensively than the resources of biogas. However, in the case of biogas, a significant increase in the utilization of the production potential was observed: from 3.3% in 2012 to 6.4% in 2018, whereas in the same years, the utilization of solid biomass production potential remained at the same level (15.3% in 2012, 15.4% in 2018). It was also observed that, at the level of voivodeships, the utilization of biogas and biomass production potential is negatively correlated with the size of this potential. The combined potential of solid biomass and biogas can cover the demand of each of the studied regions in Poland in terms of thermal energy. The coverage ranges from 104% to 1402%. The results show that when comparing biomass and biogas, the production of both electricity and heat was dominated by solid biomass. Its high share occurred especially in voivodeships characterized by a high share of forest area and a low potential for biogas production (Lubuskie Voivodeship, Zachodniopomorskie Voivodeship).

scholarly journals Influence of Temperature on Biogas Production Efficiency and Microbial Community in a Two-Phase Anaerobic Digestion System

Water ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 133 ◽  
Author(s):  
Shiwei Wang ◽  
Fang Ma ◽  
Weiwei Ma ◽  
Ping Wang ◽  
Guang Zhao ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Production Efficiency ◽  
Biogas Production ◽  
Two Phase ◽  
Influence Of Temperature ◽  
Influence Of Temperature On ◽  
Acidogenic Phase ◽  
Methanogenic Phase

In this study, the influence of temperature on biogas production efficiency and the microbial community structure was investigated in a two-phase anaerobic digestion reactor for co-digestion of cow manure and corn straw. The results illustrated that the contents of solluted chemical oxygen demand (SCOD) and volatile fatty acid (VFA) in the acidogenic phase and biogas production in the methanogenic phase maintained relatively higher levels at temperatures ranging from 35–25 °C. The methane content of biogas production could be maintained higher than 50% at temperatures above 25 °C. The microbial community structure analysis indicated that the dominant functional bacteria were Acinetobacter, Acetitomaculum, and Bacillus in the acidogenic phase and Cenarchaeum in the methanogenic phase at 35–25 °C. However, the performances of the acidogenic phase and the methanogenic phase could be significantly decreased at a lower temperature of 20 °C, and microbial activity was inhibited obviously. Accordingly, a low temperature was adverse for the performance of the acidogenic and methanogenic phases, while moderate temperatures above 25 °C were more conducive to high biogas production efficiency.

The valuation of malnutrition in the mono-digestion of maize silage by anaerobic batch tests

2008 ◽  
Vol 58 (7) ◽  
pp. 1453-1459 ◽  
Author(s):  
L. Hinken ◽  
I. Urban ◽  
E. Haun ◽  
I. Urban ◽  
D. Weichgrebe ◽  
...  
Keyword(s):  
Trace Elements ◽  
Anaerobic Digestion ◽  
Trace Element ◽  
Biogas Production ◽  
Renewable Energy Sources ◽  
Anaerobic Bacteria ◽  
Trace Element Content ◽  
Energy Crops ◽  
Maize Silage ◽  
Batch Tests

Anaerobic digestion is a technology which is used to produce methane from organic solids and energy crops. Especially in recent years, the fermentation of energy crops has become more and more important because of increasing costs for energy and special benefits for renewable energy sources in Germany. Anaerobic bacteria require macro and micro nutrients to grow. Absence of these elements can inhibit the anaerobic process significantly. In particular mono-substrates like maize or certain industrial wastewater often cannot provide all required nutrients. For this reason this research investigates the influence of substrate and trace elements on anaerobic digestion in detail. Different agricultural anaerobic biomasses are analysed with special regard to their trace element content. Based on these results, the influence of three trace elements (iron, cobalt, and nickel) on anaerobic digestion was studied in anaerobic batch tests at different sludge loading rates and for different substrates (maize and acetate). Biogas production was found to be 35% for maize silage and up to 70% higher for acetate with trace element dosage than in the reference reactor.

Estimation of Biogas Production from Shrimp Pond Sediment Using the Artificial Intelligence

2012 ◽  
Vol 260-261 ◽  
pp. 695-700
Author(s):  
J. Srisertpol ◽  
P. Srinakorn ◽  
A. Kheawnak ◽  
K. Chamniprasart
Keyword(s):  
Renewable Energy ◽  
Anaerobic Digestion ◽  
Flow Rate ◽  
Environmental Planning ◽  
Biogas Production ◽  
Biochemical Properties ◽  
Shrimp Pond ◽  
Pond Sediment ◽  
Balance Principle ◽  
The Mathematical Model

A biogas production development increases renewable energy and reduces the environmental impact which is caused by carbon dioxide. Thisis important for energy and environmental planning in Thailand. The biogas production by anaerobic digestionproduces methane that can be used as renewable energy. This research was to study biogas production from the anaerobic digestion of shrimp pond sediment by the batch reaction, an estimation of the mathematical model using theArtificial Intelligence (AI) technique and the treatment of shrimp pond sediment.The mass balance principle to create mathematical modeling and decompositions of organic matter into biogas were used to compare the experimental dataincluding, temperature, pH, biogas flow rate and biochemical properties of the shrimp pond sediment. From the results, mathematical models can estimate the dynamic response of the biogas flow rate and factors that affectedthe biogas productions. The treatment of shrimp pond sediment by anaerobic digestion process could reduce TS, TDS, TSS, TVS, BOD, COD and ECby81-89%, 52-60%, 95-99%, 80-89%, 86-95% , 85-95% and 12-22 % respectively.

scholarly journals Effect of Thermal Pretreatment on the Yield of Biogas from Microcoleous Vaginatus

2021 ◽  
Vol 17 (4) ◽  
pp. 250-256
Author(s):  
M. Haruna ◽  
O.R. Momoh ◽  
S. Bilal
Keyword(s):  
Cell Wall ◽  
Anaerobic Digestion ◽  
Biogas Production ◽  
Renewable Energy Sources ◽  
Research Work ◽  
Proximate Analysis ◽  
Biogas Yield ◽  
Bacteria Growth ◽  
Positive Effect ◽  
Made In

Biomass is being looked upon as one of the promising renewable energy sources for the future, with growing interest in microalgae conversion into biogas through anaerobic digestion. Recently, the ability of microalgae to treat waste water has doubled its potentials material today. However, in spite of the progress made in that regards, there are still challenges of algae conversion to biofuel, due to the presence of complex cell wall in some algae. Cell wall inhibits bacteria growth during degradation. In this research work 10 grams of Microcoleous vaginatus was treated in an oven at varying temperatures of 70, 75 and 80 oC for an hour, out of which 4 g was measured into 250 ml serum bottle for digestion at mesophilic temperature of 37 oC. Based on the results of proximate analysis, 69%increase in carbohydrate was attained with 72.7 – 148% reduction in moisture content. The biogas yield of untreated sample was 4.36 mLg−1 VS, while, pretreated samples at 70, 75 and 80 ℃ produced 8.39, 9.07 and 9.38 mLg−1VS (volatile solid) of biogas. This  corresponds to 92, 108 and 115% higher than that of untreated samples. However, thermal treatment of M. vaginatus prior to digestion show positive effect on carbohydrate extraction and enhanced biogas and methane yield as well. Therefore, this makes the substrate a good feedstock for biogas production. Keywords: Biomass, pretreatment, thermal, anaerobic digestion, degradation, Microcoleous vaginatus.

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