scholarly journals Intensity distribution segmentation in Ultrafast Doppler and correlative Scanning Laser Confocal Microscopy for assessing vascular changes associated with ageing in murine hippocampi

2021 ◽  
Author(s):  
Maximiliano Anzibar Fialho ◽  
Lucía Vázquez ◽  
Mariana Martínez ◽  
Miguel Calero ◽  
Jerome Baranger ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Cerebral Blood Volume ◽  
Brain Injuries ◽  
Volume Fraction ◽  
Ex Vivo ◽  
Imaging Modalities ◽  
Scanning Laser ◽  
Vascular Structure ◽  
Laser Confocal Microscopy ◽  
Cross Sectional

Abstract The hippocampus plays an important role in learning and memory, requiring high-neuronal oxygenation. Understanding the relationship between blood flow and vascular structure – and how it changes with ageing – is physiologically and anatomically relevant. Ultrafast Doppler (µDoppler) and Scanning Laser Confocal Microscopy (SLCM) are powerful imaging modalities that can measure in-vivo Cerebral Blood Volume (CBV) and ex-vivo vascular structure, respectively. Here, we apply both imaging modalities to a cross-sectional and longitudinal study of hippocampi vasculature in wild-type mice brains. We introduce a segmentation of CBV distribution obtained from µDoppler and show that this mice-independent and mesoscopic measurement is correlated with the number of vessels and Vessel Volume Fraction (VVF) distributions obtained from SLCM – e.g., high CBV relates to fewer number of vessels but with large VVF. Moreover, we find significant changes in CBV distribution and vasculature due to ageing (5 vs. 21 month-old mice), highlighting the sensitivity of our approach. Overall, we are able to associate CBV with vascular structure – and track its longitudinal changes – at the artery-vein, venules, arteriole, and capillary levels. We believe that this correlative approach can be a powerful tool for studying other acute (e.g., brain injuries), progressive (e.g., neurodegeneration) or induced pathological changes.

scholarly journals Localization of cerium-based reaction products by scanning laser reflectance confocal microscopy.

1990 ◽  
Vol 38 (3) ◽  
pp. 315-318 ◽  
Author(s):  
J M Robinson ◽  
B E Batten
Keyword(s):  
Hydrogen Peroxide ◽  
Confocal Microscopy ◽  
Scanning Laser ◽  
Reaction Products ◽  
Laser Confocal Microscopy ◽  
Light Microscope Level ◽  
New Approach ◽  
Detection Systems ◽  
Laser Reflectance ◽  
Hydrogen Peroxide Release

Scanning laser confocal microscopy was utilized to visualize sites of hydrogen peroxide release from stimulated neutrophils and lysosomal acid phosphatase in these and other cells using cerium in the detection systems. Imaging of the cerium-containing reactions was achieved by employing the reflectance mode of this instrument. Localization of these products at the light microscope level was direct and did not require other reactions to generate a visible product. This new approach to cerium cytochemistry should prove useful for many applications.

In Vivo and Ex Vivo Laser Confocal Microscopy Findings in Patients With Early-stage Acanthamoeba Keratitis

Cornea ◽  
2008 ◽  
Vol 27 (4) ◽  
pp. 439-445 ◽  
Author(s):  
Akira Kobayashi ◽  
Yasuhisa Ishibashi ◽  
Yosaburo Oikawa ◽  
Hideaki Yokogawa ◽  
Kazuhisa Sugiyama
Keyword(s):  
Confocal Microscopy ◽  
Ex Vivo ◽  
Early Stage ◽  
Acanthamoeba Keratitis ◽  
Laser Confocal Microscopy

Detection of diaminobenzidine reactions using scanning laser confocal reflectance microscopy.

1989 ◽  
Vol 37 (12) ◽  
pp. 1761-1765 ◽  
Author(s):  
J M Robinson ◽  
B E Batten
Keyword(s):  
Confocal Microscopy ◽  
Horseradish Peroxidase ◽  
Scanning Laser ◽  
Reaction Products ◽  
Laser Confocal Microscopy ◽  
Confocal Reflectance Microscopy ◽  
Mouse Oocytes ◽  
Important Addition ◽  
Reflectance Microscopy ◽  
Cytochemical Technique

We used scanning laser confocal microscopy to visualize sites of peroxidatic activity as detected by the diaminobenzidine (DAB) reaction. Imaging was achieved by employing the reflectance mode of this instrument. Intense reflectance was detected after DAB localization of endogenous granule-associated myeloperoxidase in neutrophils and of the exogenous tracer horseradish peroxidase in mouse oocytes. Detection of DAB reaction products with confocal reflectance microscopy will probably be an important addition to the utility of this cytochemical technique.

Imaging cellular dynamics using scanning laser confocal microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 12-13
Author(s):  
M.S. Cooper
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Three Dimensional ◽  
Optical Systems ◽  
Scanning Laser ◽  
Fluorescent Light ◽  
Physiological Changes ◽  
Incident Laser ◽  
Laser Confocal Microscopy ◽  
Image Planes

In recent years, the ability to image morphological dynamics and physiological changes in living cells and tissues has been greatly advanced by the advent of scanning laser confocal microscopy. Confocal microscopes employ optical systems in which both the condenser and objective lenses are focused onto a single volume element of the specimen. In practice, galvanometer-driven mirrors or acousto-optical deflectors are used to scan a laser beam over the specimen in a raster-like fashion through an epifluorescence microscope. The incident laser beam, as well as the collected fluorescent light, are passed through pinhole or slit apertures in image planes that are conjugate to the plane of the specimen. This method of illumination and detection prevents fluorescent light which is generated above and below the plane-of-focus from impinging on the imaging system's photodetector, thus rejecting much of the fluorescent light which normally blurs the image of a three-dimensional fluorescent specimen.

Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

2004 ◽  
Author(s):  
Mohamed-Adel Slamani ◽  
Andrzej Krol ◽  
Jacques Beaumont ◽  
Robert L. Price ◽  
Ioana L. Coman ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Three Dimensional ◽  
Scanning Laser ◽  
Three Dimensional Reconstruction ◽  
Laser Confocal Microscopy ◽  
Dimensional Reconstruction

scholarly journals In vivo evaluation of DSAEK interface with scanning-laser confocal microscopy

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Giulio Ferrari ◽  
Verena Reichegger ◽  
Luca Ludergnani ◽  
Elisabetta Delfini ◽  
Claudio Macaluso
Keyword(s):  
Confocal Microscopy ◽  
Scanning Laser ◽  
Laser Confocal Microscopy ◽  
In Vivo Evaluation

An Evaluation of Decorative Techniques on a Red-Figure Attic Vase from the Worcester Art Museum using Reflectance Transformation Imaging (RTI) and Confocal Microscopy with a Special Focus on the “Relief Line”

2011 ◽  
Vol 1319 ◽  
Author(s):  
Paula Artal-Isbrand ◽  
Philip Klausmeyer ◽  
Winifred Murray
Keyword(s):  
Confocal Microscopy ◽  
Art Museum ◽  
Confocal Microscope ◽  
Scanning Laser ◽  
Special Focus ◽  
Laser Confocal Microscopy ◽  
Ancient Technology ◽  
Surface Examination ◽  
Tools And Techniques ◽  
Reflectance Transformation Imaging

ABSTRACTDecorative features on a Greek red-figure stamnos in the collection of the Worcester Art Museum were examined using Reflectance Transformation Imaging (RTI) and scanning laser confocal microscopy. These two surface examination tools helped to answer questions relating to the decorative process, particularly the tools and techniques that Attic painters used to create the so-called glossy black “relief lines” and “relief dots.” This research also incorporated fabricated mock-ups to help understand the ancient technology. It was determined that the relief line was not produced by an extruded method, but with a brush made of one or very few hairs, an idea first proposed by Gérard Seiterle in 1976 and termed Linierhaar. It was observed that not one but two distinct types of relief lines exist: the “laid” line (proposed by Seiterle) characterized by a ridge running through the middle of the line and the “pulled” line (proposed in this paper) which has a furrowed profile. Both line types were reproduced with a Linierhaar. Additionally, relief dots were replicated using a conventional brush. Surface examinations of other red-figure vessels using RTI and the confocal microscope suggest these conclusions apply to vessels of this genre as a whole.

Evaluation of Prostaglandin Analogue Effects on Corneal Keratocyte Density Using Scanning Laser Confocal Microscopy

2010 ◽  
Vol 19 (9) ◽  
pp. 617-621 ◽  
Author(s):  
Chiara Bergonzi ◽  
Andrea Giani ◽  
Mirella Blini ◽  
Sylvia Marchi ◽  
Saverio Luccarelli ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Scanning Laser ◽  
Prostaglandin Analogue ◽  
Laser Confocal Microscopy ◽  
Keratocyte Density

Backside Infrared Imaging Using Improved Refraction-Assisted Illumination Methods

2010 ◽  
Author(s):  
Terence Yeoh ◽  
Stephen LaLumondiere ◽  
Neil Ives ◽  
Martin Leung
Keyword(s):  
Confocal Microscopy ◽  
Infrared Imaging ◽  
Scanning Laser ◽  
High Contrast ◽  
Uniform Illumination ◽  
Laser Confocal Microscopy ◽  
Image Composition ◽  
Microelectronic Devices

Abstract Infrared backside imaging using refraction assisted illumination (RAIL) allows for imaging microelectronic devices at high contrast and resolution through 700 microns of silicon without the need for specialized optics or coatings. However, due to the angle of illumination, not all of the metal features are equally illuminated. In this paper we present improvements on this method through image composition of a series of RAIL images taken at various illumination directions. The resultant composited image provides more uniform illumination and better approximates the features seen in scanning laser confocal microscopy.

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