scholarly journals Effect of Different Preparation Conditions On The Properties of Bamboo Fiber-Based Bioactive Composite Membrane

2022 ◽  
Author(s):  
Jiang Liuyun ◽  
Bingli Ma ◽  
Zhihong Jiang ◽  
Yingjun Ma ◽  
Yue Wen ◽  
...  
Keyword(s):  
Composite Membrane ◽  
Composite Membranes ◽  
Bamboo Fiber ◽  
Bone Tissue Regeneration ◽  
Drying Methods ◽  
X Ray Diffraction ◽  
Casting Technique ◽  
Forming Mechanism ◽  
Preparation Conditions

Abstract A novel nano-hydroxyapatite/bamboo fiber (n-HA/BF) bioactive composite membrane was obtained by a simple casting technique. The membrane forming mechanism and the effects of different forming membrane methods, drying methods and n-HA amounts on the properties of n-HA/BF membrane were investigated by Fourier Transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), contact angle, electromechanical universal tester, in vitro soaking in simulated body fluid (SBF) and in vitro cell cultureexperiment. The results demonstrated that the n-HA dispersity in BF matix was not affected by the prepartion condition, however, the morphologies of membrane was determined by the different preparation conditions owing to different hydrogen bond shrinkage. Moreover, the hydrophilicity of the composite membrane was improved under the condition of the membrane formation in oven, freeze drying and high addition content of n-HA, and the mechanical properties of composite membrane depended on n-HA content. In vitro soaking behavior indicated that the degradability and bone-like apatite deposition could be controled by differentpreparation conditions. And the cell proliferation experiment showed that the n-HA/BF composite membranes obtained under different preparation conditions were all non-toxic. The above results indicated that the n-HA/BF composite membrane could be obtained by a simple casting technique, and the properties could be controlled by adopting different preparation conditions, which would have a great promising as guide bone tissue regeneration (GBR) membrane, and the study would provide a new application for BF in biomedical field.

DEVELOPMENT AND CHARACTERIZATION OF NOVEL GASTRORETENTIVE RAFT FORMING FLOATING FILM OF ATENOLOL

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (03) ◽  
pp. 15-23
Author(s):  
N Sharma ◽  
◽  
R. Awasthi
Keyword(s):  
Drug Release ◽  
Polymer Matrix ◽  
Processing Parameters ◽  
Dissolution Profile ◽  
X Ray Diffraction ◽  
Casting Technique ◽  
Floating Raft ◽  
Weight Variation ◽  
Folding Endurance

The aim of present work was to develop a gastroretentive floating raft forming film of atenolol using solvent casting technique. The films were characterized in terms of drug-excipient compatibility by FTIR, drug content, swelling, folding endurance, thermal behaviour by DSC, effect of processing parameters on drug state (amorphous or crystalline) by X-ray diffraction (XRD), and in vitro drug release profiles. The results confirm that there was no interaction between the drug-polymers and fusion of drug crystals within the polymer matrix. Results of XRD indicate partial dissolution of drug within the polymer matrix and suggested it was partly distributed in amorphous form throughout the film. The weight variation, thickness and folding endurance of films were in the range of 2.170 ± 0.05 to 2.444 ± 0.23 gm, 1.120 ± 0.032 to 1.125 ± 0.011 mm and 200 ± 5 to 400 ± 5, respectively. The pH values of the different films were between 6.8 to 7.21. After 24 h, the best selected film shows 75% and 90% of drug release in 0.1 N HCl (pH 1.2) and in phosphate buffer (pH 6.8), respectively. Based on these results it is suggested that the incorporation of drug into the hydrophilic floating film may be an appropriate strategy to improve the dissolution profile and oral bioavailability of the drug.

scholarly journals Preparation and Bioactivity Properties of a Novel Composite Membrane of Fructose Mediatedβ-Tricalcium Pyrophosphate/(Polyethylene Glycol/Chitosan) for Guided Tissue Regeneration

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Jian-Wen Wang ◽  
Min-Hsiung Hon ◽  
Yi-Ming Kuo ◽  
Mei-Hui Chung
Keyword(s):  
Mechanical Property ◽  
Tissue Regeneration ◽  
Composite Membrane ◽  
Degradation Rate ◽  
Composite Membranes ◽  
Guided Tissue Regeneration ◽  
Glycol Chitosan ◽  
Cell Compatibility ◽  
Suitable Material

A novel composite membrane ofβ-tricalcium pyrophosphate (β-TCP) and fructose- (F-) mediated chitosan/poly(ethylene glycol) (CS/PEG) was prepared by thermally induced phase separation technique. The prepared composite membranes were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanical property, swelling, degradation, and cytotoxicity of the composite membranes were evaluated in vitro with respect to its potential for use as biodegradable guided tissue regeneration (GTR) membrane. In vitro degradation tests showed the composite membrane with a controllable degradation rate when changing theβ-TCP content. The incorporation ofβ-TCP granules also caused a significant enhancement of tensile strength. Whenβ-TCP content is controlled to 50 wt%, homogeneous composite membranes with well mechanical property and enzymatic degradation rate can be obtained. Cytotoxicity assay demonstrates that the composite membranes were nontoxic and had very good cell compatibility. Most importantly, the release of calcium ions and glucosamine from the composite membranes was proved to increase the cell proliferation of NIH3T3. The results of this study have indicated that this novel F-β-TCP/CS/PEG composite can be a suitable material for GTR applications.

scholarly journals In Vitro Hydroxyapatite-Forming Ability and Antimicrobial Properties of Mesoporous Bioactive Glasses Doped with Ti/Ag

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Huiming Lin ◽  
Jing Zhang ◽  
Fengyu Qu ◽  
Jingjie Jiang ◽  
Pingping Jiang
Keyword(s):  
Antimicrobial Properties ◽  
Textural Properties ◽  
Bone Tissue Regeneration ◽  
X Ray Diffraction ◽  
Bioactive Glasses ◽  
X Ray ◽  
Adsorption Desorption ◽  
Improved Characteristics ◽  
Regeneration Systems

Mesoporous bioactive glasses (MBGs) doped with Ti/Ag were synthesized. The structural, morphological, and textural properties of all samples were investigated by small-angle X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, andN2adsorption-desorption technique. In vitro hydroxyapatite- (HAP-) induced growth and antimicrobial properties of these materials were investigated in detail. These results indicate that MBGs-Ti/Ag possess faster HAP-induced growth and higher sterilization rate than the pure MBGs materials. With Ti/Ag doping, these functional MBGs show the improved characteristics and have more promising potential in bone-tissue regeneration systems and surgery.

scholarly journals High-Hydrophilic and Antifouling Poly (Vinylidene Fluoride) Composite Membranes via in Situ Mosaic- Assembled Nanocrystalline Cellulose and g-C3N4

2021 ◽  
Author(s):  
Hongbin Li ◽  
chao liu ◽  
Yongqiang Guo ◽  
Shuzhen Gao
Keyword(s):  
Tensile Strength ◽  
Composite Membrane ◽  
Vinylidene Fluoride ◽  
Water Flux ◽  
Composite Membranes ◽  
Nanocrystalline Cellulose ◽  
Membrane Properties ◽  
X Ray Diffraction ◽  
Poly Vinylidene Fluoride

Abstract Developing an antifouling and stable separation poly (vinylidene fluoride) (PVDF) membrane for water treatment is of great significance but challenging due to the limitations of its low surface properties and strong hydrophobicity. In this study, a novel multi-block composite ultrafiltration membrane was developed using the mosaic-assembled doping of pineapple leaf nanocrystalline cellulose and g-C3N4. The effects of adding different components on the PVDF composite membrane properties have been analyzed. The surface chemical composition, surface morphology, crystallinity and thermal stability of the composite membranes were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). Both of the tensile strength and elongation length of the PVDF composite membranes were enhanced due to the addition of pineapple leaf nanocellulose and g-C3N4, and the tensile strength and elongation length of PVDF/PEG/g-C3N4/Pineapple leaf nanocellulose composite membrane can reach 10.61 MPa and 8.85 mm. The porosity of the PVDF composite membranes was 46.6%, respectively. The water flux and flux recovery ratio of PVDF/PEG/g-C3N4/Pineapple leaf nanocellulose also can reach 256.75 L/(m2 ⋅h) and up to 82.1%. All the above experimental data showed that the addition of pineapple leaf nanocellulose and g-C3N4 can greatly improve the performance of the PVDF composite membrane. The prepared modified membrane has potential application value in the field of wastewater separation and treatment.

scholarly journals Titania Nanofiber Scaffolds with Enhanced Biointegration Activity—Preliminary In Vitro Studies

2019 ◽  
Vol 20 (22) ◽  
pp. 5642 ◽  
Author(s):  
Ehlert ◽  
Roszek ◽  
Jędrzejewski ◽  
Bartmański ◽  
Radtke
Keyword(s):  
Cell Viability ◽  
Bone Tissue ◽  
Chemical Oxidation ◽  
Bone Tissue Regeneration ◽  
High Porosity ◽  
X Ray Diffraction ◽  
Bioactive Materials ◽  
Tio2 Nanofibers ◽  
Tissue Cells

The increasing need for novel bone replacement materials has been driving numerous studies on modifying their surface to stimulate osteogenic cells expansion and to accelerate bone tissue regeneration. The goal of the presented study was to optimize the production of titania-based bioactive materials with high porosity and defined nanostructure, which supports the cell viability and growth. We have chosen to our experiments TiO2 nanofibers, produced by chemical oxidation of Ti6Al4V alloy. Fibrous nanocoatings were characterized structurally (X-ray diffraction (XRD)) and morphologically (scanning electron microscopy (SEM)). The wettability of the coatings and their mechanical properties were also evaluated. We have investigated the direct influence of the modified titanium alloy surfaces on the survival and proliferation of mesenchymal stem cells derived from adipose tissue (ADSCs). In parallel, proliferation of bone tissue cells—human osteoblasts MG-63 and connective tissue cells - mouse fibroblasts L929, as well as cell viability in co-cultures (osteoblasts/ADSCs and fibroblasts/ADSCs has been studied. The results of our experiments proved that among all tested nanofibrous coatings, the amorphous titania-based ones were the most optimal scaffolds for the integration and proliferation of ADSCs, fibroblasts, and osteoblasts. Thus, we postulated these scaffolds to have the osteopromotional potential. However, from the co-culture experiments it can be concluded that ADSCs have the ability to functionalize the initially unfavorable surface, and make it suitable for more specialized and demanding cells.

Bioactivity of TiO2/Ti Composite Membrane with Different Crystral Phase

2011 ◽  
Vol 287-290 ◽  
pp. 69-72
Author(s):  
Min Jing Zhan ◽  
Gang Li ◽  
Qiang Wei ◽  
Hua Lei Cui ◽  
Ling Lin
Keyword(s):  
Composite Membrane ◽  
Anatase Phase ◽  
Sol Gel ◽  
Composite Membranes ◽  
Rutile Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Neural Net ◽  
Hypothalamic Neurons ◽  
Porous Ti

The bioactivity of TiO2/Ti composite membrane with different crystral phase was studied by the culture of hypothalamic neurons in vitro for the application of immunoisolation. The kind of membrane supported on porous Ti has been prepared by sol-gel technique and the crystral phase was controlled by different sintering temperature. The crystral phase of TiO2, morphology and function of hypothalamic neurons were observed by X-ray diffraction (XRD), enzyme-linked immunosorbent assay (ELISA) and environmental sanning electron microscope (ESEM). The results showed that neurons could attach well to the TiO2 /Ti composite membranes with rutile phase and became bipolar. The long axon and dendrite with dendrite spine were interconnected by synapses. A complicated neural net were easily observed. The secretory level of β-endorphine remained between 51.3pmol/L and 40.6pmol/L all the times. However, the neurons in membranes with anatase were transformed into an umbilicate structure without processes. The secretory function of neurons also died out in 4 days. These results indicated that the TiO2 /Ti composite membranes with rutile phase were propitious to the neuron’s growth than the composite membranes with anatase phase and could be used as immunoisolation membranes.

Castability and Biocompatibility of Novel Fluorcanasite Glass-Ceramics

2006 ◽  
Vol 309-311 ◽  
pp. 293-296 ◽  
Author(s):  
S. Bandyopadhyay-Ghosh ◽  
Ian M. Reaney ◽  
A. Johnson ◽  
I.M. Brook ◽  
K. Hurrell-Gillingham ◽  
...  
Keyword(s):  
Stoichiometric Composition ◽  
Glass Ceramics ◽  
Casting Process ◽  
X Ray Diffraction ◽  
Form Complex ◽  
X Ray ◽  
Casting Technique ◽  
In Vitro Biocompatibility ◽  
Complex Shapes

Novel fluorcanasite based glass-ceramics were produced by controlled two stage heattreatment of as-cast glasses. Castability of parent glasses was determined using a graduated spiral cast piece. Fluorcanasite glasses were also cast to form complex shapes using the lost wax casting technique. Gypsum and phosphate bonded investments were used to investigate their effect on the casting process, cast surface crystallinity and biocompatibility. The stoichiometric composition had the greatest castability but the other two modified compositions also had good castability. X-ray diffraction showed similar bulk crystallisation for each glass irrespective of the investment material. However, some differences in surface crystallisation in the presence of different investment materials were detected. Discs cast using gypsum bonded investment showed greater in vitro biocompatibility than equivalent discs cast using phosphate bonded investment under the conditions used. Gypsum and phosphate bonded investments could both be successfully used for the lost wax casting of these novel fluorcanasite glasses.

IN VITROCHARACTERIZATION OF TRAMADOL-HCL-LOADED POLYOXALATE MICROSPHERES DESIGNED FOR CONTROLLED DRUG RELEASE

2015 ◽  
Vol 27 (03) ◽  
pp. 1550022
Author(s):  
Cheon Jung Lee ◽  
Su Young Kim ◽  
Hyun Gu Lee ◽  
Jaewon Yang ◽  
Jin Young Park ◽  
...  
Keyword(s):  
Drug Release ◽  
High Performance ◽  
Controlled Drug Release ◽  
Physicochemical Characteristics ◽  
In Vitro Test ◽  
X Ray Diffraction ◽  
Sustained Drug Release ◽  
Release Formulation ◽  
Preparation Conditions

This study evaluates the properties of Tramadol- HCl -loaded polyoxalate (TH-loaded POX) microspheres prepared by oil-in-oil (O1/O2) emulsion solvent evaporation method, specifically designed for sustained drug release. Morphology and physicochemical characteristics of the as-fabricated were studied by scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimeter (DSC) and Fourier transform infrared (FTIR) spectroscopy, while the encapsulation efficiency and release profile of drug (Tramadol- HCl , TH) from POX microspheres were assessed by high-performance liquid chromatography (HPLC). The influence of reaction temperature, stirring speed, initial drug ratio, molecular weight (Mw) and concentration of polyoxalate (POX) on the fabrication of TH-loaded POX microspheres were investigated. Results showed that the characteristics of the microspheres and drug-loaded content can be optimized by adjusting the parameters of preparation conditions. Also, the degradation behavior of TH-loaded POX microspheres was evaluated from in vitro test for 2 weeks. Overall, the results showed that POX microsphere can be one of the promising polymers for controlled injection release formulation with site-specific drug release capabilities.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

The 3-Dimensional Molecular Structure of the Actin Filament Revisited

1985 ◽  
Vol 43 ◽  
pp. 480-481
Author(s):  
U. Aebi ◽  
R. Millonig ◽  
H. Salvo
Keyword(s):  
Actin Filament ◽  
Supramolecular Assembly ◽  
Specimen Preparation ◽  
X Ray Diffraction ◽  
Single Filament ◽  
X Ray ◽  
3 Dimensional ◽  
Filament Diameter ◽  
Preparation Conditions ◽  
Apparent Diameter

To date, most 3-D reconstructions of undecorated actin filaments have been obtained from actin filament paracrystal data (for refs, see 1,2). However, due to the fact that (a) the paracrystals may be several filament layers thick, and (b) adjacent filaments may sustantially interdigitate, these reconstructions may be subject to significant artifacts. None of these reconstructions has permitted unambiguous tracing or orientation of the actin subunits within the filament. Furthermore, measured values for the maximal filament diameter both determined by EM and by X-ray diffraction analysis, vary between 6 and 10 nm. Obviously, the apparent diameter of the actin filament revealed in the EM will critically depend on specimen preparation, since it is a rather flexible supramolecular assembly which can easily be bent or distorted. To resolve some of these ambiguities, we have explored specimen preparation conditions which may preserve single filaments sufficiently straight and helically ordered to be suitable for single filament 3-D reconstructions, possibly revealing molecular detail.

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