Biosynthesis Zinc Oxide Nanoparticles Using Senna Occidentalis Leaf Extract and Evaluation of their Cytotoxic Effect on SW480 Colon Cancer Cell Line

Abstract ObjectiveIn the present study, we green synthesized ZnO NPs (zinc oxide nanoparticles) from Senna occidentalis leaf extract which were subsequently assessed for their cytotoxic and antioxidant activity on colon cancer SW480 cell line.Results Zinc oxide nanoparticles were characterized by using UV-Vis spectroscopy, X-ray diffractometer (XRD), Particle size analyzer (PSA), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FE-SEM), Energy-dispersive X-ray (EDS) and atomic force microscope (AFM) analysis. The PSA, XRD and AFM showed 20-50 nm size and nearly cuboidal and irregular shaped of the ZnO NPs. The synthesized ZnO nanoparticles were evaluated for their anticancer activity on human colon cancer cell line (SW480) by using MTT and neutral red uptake assay. The SW480 colon cancer cells were treated with various concentrations of ZnO NPs in the range of 20–100 µg/ml for 2 hrs. The result showed that ZnO NPs could reduce cell viability of SW480 cells up to 50% at the concentration of 100 µg/ml and induce membrane leakage in a concentration dependent manner.ConclusionThe anticancer activity of zinc oxide nanoparticles has showed that these can be used as effective anti-cancer agent against colon cancer cell lines (SW480).

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Biosynthesis of cerium oxide nanoparticles and its cytotoxicity survey against colon cancer cell line

Applied Organometallic Chemistry ◽

10.1002/aoc.5308 ◽

2019 ◽

Vol 34 (1) ◽

Cited By ~ 6

Author(s):

Abdolhossein Miri ◽

Majid Darroudi ◽

Mina Sarani

Keyword(s):

Colon Cancer ◽

Cell Line ◽

Cerium Oxide ◽

Cancer Cell ◽

Cancer Cell Line ◽

Colon Cancer Cell Line ◽

Colon Cancer Cell ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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Green Synthesis of Zinc Oxide Nanoparticles (ZnO NPs) using Aqueous Extract of Tagetes erecta flower and Evaluation of its Antioxidant, Antimicrobial, and Cytotoxic activities on HeLa cell line.

Current Biotechnology ◽

10.2174/2211550109999201202123939 ◽

2020 ◽

Vol 09 ◽

Author(s):

Akila Ilangovan ◽

Akshaya Venkatramanan ◽

Pakutharivu Thangarajan ◽

Anitha Saravanan ◽

Sowndarya Rajendran ◽

...

Keyword(s):

Antimicrobial Activity ◽

Zinc Oxide ◽

Cell Line ◽

Cancer Cell ◽

Crude Extract ◽

Zinc Oxide Nanoparticles ◽

Cancer Cell Line ◽

Tagetes Erecta ◽

Oxide Nanoparticles ◽

Zno Nps

Background: The Zinc oxide Nanoparticles (ZnO NPs) were synthesized successfully by using Tagetes erecta flower aqueous extract and evaluated for their antioxidant potential, antimicrobial and cytotoxic potential. Methods: Phytochemical screening of aqueous crude extract and synthesized ZnO NPs of Tagetes erecta flower revealed the presence of alkaloids, flavonoids, carbohydrates, amino acids, tannins, and proteins, etc. The characterization was done by various spectral analyses. Invitro antioxidant activities of synthesized ZnO NPs were found to possess concentrationdependent free radical scavenging activity was carried on different free radicals i.e. DPPH and ABTS. Antimicrobial activity of synthesized ZnO NPs was done by agar well diffusion method and compared with control ampicillin, while cytotoxic effects were determined by MTT assay against human cervical cancer cell line. Results: UV-Visible spectra were conducted to confirm the synthesis of ZnO NPs and peak obtained at 364.15nm. X-ray analysis confirmed the crystalline nature of the nanoparticles and the average size of the nanoparticles was 30-50nm and was spherical shape analyzed by SEM. The synthesized ZnO NPs showed antimicrobial activity against all tested microorganisms and maximum inhibition zone was found against E.coli in gram-negative and S.aureus in gram-positive bacteria. Synthesized ZnO NPs were showed 50% cell viability at 26.53µg/ml against the HeLa cancer cell line. Conclusion: The conclusion of this study suggests both the aqueous crude extract of Tagetes erecta flower and synthesized ZnO NPs showed an excellent alternative source of antimicrobial agent also an attractive selective cytotoxic activity against HeLa tested cancer lines, offering satisfying ‘safe and cheaper’ alternatives to conventional therapy protocols.

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