scholarly journals A Single Nucleotide Polymorphism in Promoter of Porcine ARHGAP24 Gene Regulates Aggressive Behavior of Weaned Pigs After Mixing by Affecting the Binding of Transcription Factor P53

2022 ◽  
Author(s):  
Qinglei Xu ◽  
Yanli Guo ◽  
Jing Zhao ◽  
Mingzheng Liu ◽  
Allan P. Schinckel ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Aggressive Behavior ◽  
Axon Guidance ◽  
Animal Welfare ◽  
Promoter Region ◽  
Transcriptional Activity ◽  
Weaned Pigs ◽  
Single Nucleotide ◽  
Behavioral Traits ◽  
Transcription Factor P53

Abstract Background: Weaned pigs often have more aggressive behavior after mixing, which has negative effects on animal welfare and growth performance. Identification of functional single nucleotide polymorphisms (SNPs) related to aggressive behavior of pigs would provide valuable molecular markers of aggressive behavioral trait for genetic improvement program. Rho GTPase Activating Protein 24 (ARHGAP24) gene plays an important role in regulating the process of axon guidance, which may impact aggressive behavior of pigs. Results: By re-sequencing the entire coding region, partially adjacent introns and the 5’ and 3’ flanking regions, 6 and 4 SNPs were identified in the 5’ flanking region and 5’ untranslated region (UTR) of porcine ARHGAP24 gene, respectively. Association analyses revealed that 9 SNPs were significantly associated with aggressive behavioral traits (P = < 1.00 × 10−4 - 4.51 × 10−2), and their haplotypes were significantly associated with aggressive behavior (P = < 1.00 × 10−4 - 2.99 × 10−2). The core promoter region of ARHGAP24 gene was identified between -670 bp and -1113 bp. Furthermore, the luciferase activity of allele A of rs335052970 was significantly less than that of allele G, suggesting the transcriptional activity of ARHGAP24 gene was inhibited by allele A of rs335052970. It was identified that the transcription factor p53 bound to the transcription factor binding sites (TFBSs) containing allele A of rs335052970. In porcine primary neural cells, p53 bind to the target promoter region of ARHGAP24 gene, reduce its promoter transcriptional activity, and then reduce its messenger RNA (mRNA) and protein expression through axon guidance pathway.Conclusion: The results demonstrated that ARHGAP24 gene had significant genetic effects on aggressive behavioral traits of pigs. Therefore, rs335052970 in ARHGAP24 gene can be used as a molecular marker to select less aggressive pigs and improve animal welfare.

scholarly journals Runt-Related Transcription Factor 1 Regulates Luteinized Hormone-Induced Prostaglandin-Endoperoxide Synthase 2 Expression in Rat Periovulatory Granulosa Cells

Endocrinology ◽  
2009 ◽  
Vol 150 (7) ◽  
pp. 3291-3300 ◽  
Author(s):  
Jing Liu ◽  
Eun-Sil Park ◽  
Misung Jo
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Granulosa Cells ◽  
Promoter Region ◽  
Transcriptional Activity ◽  
Binding Motifs ◽  
Related Transcription Factor ◽  
Prostaglandin Endoperoxide Synthase ◽  
Transcription Factor 1 ◽  
Runx1 Expression

Runt-related transcription factor 1 (RUNX1), a transcription factor, is transiently induced by the LH surge and regulates gene expression in periovulatory granulosa cells. Potential binding sites for RUNX are present in the 5′-flanking region of the Ptgs2 (prostaglandin-endoperoxide synthase 2) gene. Periovulatory Ptgs2 expression is essential for ovulation. In the present study, we investigated the role of RUNX1 in mediating the LH-induced expression of Ptgs2 in periovulatory granulosa cells. We first determined whether the suppression of Runx1 expression or activity affects Ptgs2 expression using cultured preovulatory granulosa cells isolated from immature rat ovaries primed with pregnant mare serum gonadotropin for 48 h. Knockdown of human chorionic gonadotropin-induced Runx1 expression by small interfering RNA or inhibition of endogenous RUNX activities by dominant-negative RUNX decreased human chorionic gonadotropin or agonist-stimulated Ptgs2 expression and transcriptional activity of Ptgs2 promoter reporter constructs. Results from chromatin immunoprecipitation assays revealed in vivo binding of endogenous RUNX1 to the Ptgs2 promoter region in rat periovulatory granulosa cells. Direct binding of RUNX1 to two RUNX-binding motifs in the Ptgs2 promoter region was confirmed by EMSA. The mutation of these two binding motifs resulted in decreased transcriptional activity of Ptgs2 promoter reporter constructs in preovulatory granulosa cells. Taken together, these findings provide experimental evidence that the LH-dependent induction of Ptgs2 expression results, in part, from RUNX1-mediated transactivation of the Ptgs2 promoter. The results of the present study assign potential significance for LH-induced RUNX1 in the ovulatory process via regulating Ptgs2 gene expression.

scholarly journals Responses of HSP70 Gene to Vibrio parahaemolyticus Infection and Thermal Stress and Its Transcriptional Regulation Analysis in Haliotis diversicolor

Molecules ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 162 ◽  
Author(s):  
Zhiqiang Fang ◽  
Yulong Sun ◽  
Xin Zhang ◽  
Guodong Wang ◽  
Yuting Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Thermal Stress ◽  
Vibrio Parahaemolyticus ◽  
Promoter Region ◽  
Transcriptional Activity ◽  
Core Promoter ◽  
Haliotis Diversicolor ◽  
Core Promoter Region ◽  
Directed Mutation

Heat-shock protein 70 (HSP70) is a molecular chaperone that plays critical roles in cell protein folding and metabolism, which helps to protect cells from unfavorable environmental stress. Haliotis diversicolor is one of the most important economic breeding species in the coastal provinces of south China. To date, the expression and transcriptional regulation of HSP70 in Haliotis diversicolor (HdHSP70) has not been well characterized. In this study, the expression levels of HdHSP70 gene in different tissues and different stress conditions were detected. The results showed that the HdHSP70 gene was ubiquitously expressed in sampled tissues and was the highest in hepatopancreas, followed by hemocytes. In hepatopancreas and hemocytes, the HdHSP70 gene was significantly up-regulated by Vibrio parahaemolyticus infection, thermal stress, and combined stress (Vibrio parahaemolyticus infection and thermal stress combination), indicating that HdHSP70 is involved in the stress response and the regulation of innate immunity. Furthermore, a 2383 bp of 5′-flanking region sequence of the HdHSP70 gene was cloned, and it contains a presumed core promoter region, a CpG island, a (TG)39 simple sequence repeat (SSR), and many potential transcription factor binding sites. The activity of HdHSP70 promoter was evaluated by driving the expression of luciferase gene in HEK293FT cells. A series of experimental results indicated that the core promoter region is located between −189 bp and +46 bp, and high-temperature stress can increase the activity of HdHSP70 promoter. Sequence-consecutive deletions of the luciferase reporter gene in HEK293FT cells revealed two possible promoter activity regions. To further identify the binding site of the key transcription factor in the two regions, two expression vectors with site-directed mutation were constructed. The results showed that the transcriptional activity of NF-1 site-directed mutation was significantly increased (p < 0.05), whereas the transcriptional activity of NF-κB site-directed mutation was significantly reduced. These results suggest that NF-1 and NF-κB may be two important transcription factors that regulate the expression of HdHSP70 gene.

Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5

2020 ◽  
Vol 319 (6) ◽  
pp. H1380-H1386
Author(s):  
Guangrui Lai ◽  
Leitong Wang ◽  
Zhen Li ◽  
Yanyan Zhao
Keyword(s):  
Transcription Factor ◽  
Promoter Region ◽  
Transcriptional Activity ◽  
Homeobox Transcription Factor

We found that Hcy and IGFBP5 were increased, whereas NKX2.5 was decreased, in AF of CHD. Meanwhile, Hcy could upregulate IGFBP5 but downregulate NKX2.5, and IGFBP5 inhibited NKX2.5 expression in vitro. Moreover, IGFBP5 can bind to the NKX2.5 promoter region and reduce NKX2.5 transcriptional activity.

scholarly journals Identification of Single Nucleotide Polymorphisms in Porcine MAOA Gene Associated with Aggressive Behavior of Weaned Pigs after Group Mixing

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 952 ◽  
Author(s):  
Ruonan Chen ◽  
Qingpo Chu ◽  
Chunyan Shen ◽  
Xian Tong ◽  
Siyuan Gao ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Aggressive Behavior ◽  
Promoter Activity ◽  
Monoamine Oxidase A ◽  
Luciferase Reporter ◽  
Nucleotide Polymorphisms ◽  
Weaned Pigs ◽  
Single Nucleotide ◽  
Mutant Genotype ◽  
Maoa Gene

Understanding the genetic background underlying the expression of behavioral traits has the potential to fasten the genetic progress for reduced aggressive behavior of pigs. The monoamine oxidase A (MAOA) gene is known as the “warrior” gene, as it has been previously linked to aggressive behavior in humans and livestock animals. To identify single nucleotide polymorphisms in porcine MAOA gene associated with aggressive behavior of pigs, a total of 500 weaned pigs were selected and mixed in 51 pens. In each pen, two aggressive and two docile pigs (a total of 204 pigs) were selected based on their composite aggressive score (CAS). Ear tissue was sampled to extract genomic DNA. Constructs containing variable lengths of truncated porcine MAOA promoter were used to determine the promoter activity by a dual luciferase reporter system. The core promoter region was located at −679 bp to −400 bp. A total of nine single nucleotide polymorphisms (SNPs) in MAOA gene were genotyped, of which six SNPs had significant differences (p < 0.05) in allele frequency between the aggressive and docile pigs. Linkage disequilibrium and association analyses showed that the pigs inherited the wild genotypes showed more aggressive behavior (p < 0.05) than pigs with the mutant genotypes of the four linked SNPs, rs321936011, rs331624976, rs346245147, and rs346324437. In addition, pigs of GCAA haplotype were more (p < 0.05) aggressive than the pigs with GCGA or ATGG haplotype. The construct containing the wild genotype GG of rs321936011 had lower (p = 0.031) promoter activity compared to the mutant genotype AA. These results suggest that the four linked SNPs in MAOA gene could be considered as a molecular marker for behavioral trait selection in pigs.

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