scholarly journals Engineered hybrid insecticidal protein improves resistance to lepidopteran insects in rice (Oryza sativa L.) and maize (Zea mays L.)

Author(s):  
Yang Liu ◽  
Siping Han ◽  
Shuo Yang ◽  
Ziqi Chen ◽  
Yuejia Yin ◽  
...  

Abstract Though cry gene transformed crops have successfully revolutionized modern agriculture, it is still necessary to discover new Cry proteins to overcome potential threatens from the development of resistant insect populations. We swapped domain-IIIs with various Cry proteins and engineered seven chimeric proteins, aiming to produce new engineered hybrid insecticidal proteins. Seven recombinant proteins were expressed in Escherichia coli. Three proteins exhibited high toxicity against Asian corn borer in dietary exposure assays. Three hybrid proteins were further transformed to rice (cv. Jijing88) to determine their insecticidal activity. Cry1Ab/Gc hybrid proteins, Cry1Ab being replaced by the domain-III of Cry1Gc, showed significantly more toxic against rice stem borer than others. Furthermore, Cry1Ab/Gc gene was transformed into maize (cv. HiII), then backcrossed into commercial maize inbred lines (cv. Ji853 and Y822), and formulated into Xiangyu 998 hybrid to evaluate their commercial value. Transgenic maize performed significant resistance improvement to the Asian corn borer without affecting the yield, and this new protein did not have adverse effects on the environment. Our result proved domain-swapped could be used as an efficient method for exploring new cry genes and engineered hybrid insecticidal protein. Cry1Ab/Gc provides a new tool for Lepidopteran insects resistant management in rice and maize.

2019 ◽  
Vol 22 (2) ◽  
pp. 493-498 ◽  
Author(s):  
Duc Khanh Le ◽  
Quang Khai Le ◽  
Thi Thuy Hang Tran ◽  
Duc Viet Nguyen ◽  
Thi Hang Dao ◽  
...  

2020 ◽  
Vol 153 ◽  
pp. 88-99
Author(s):  
Sivaprasath Prabu ◽  
Dapeng Jing ◽  
Muhammad Zeeshan Shabbir ◽  
Weining Yuan ◽  
Zhenying Wang ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0136430 ◽  
Author(s):  
Dongmei Feng ◽  
Zhen Chen ◽  
Zhiwen Wang ◽  
Chunlu Zhang ◽  
Kanglai He ◽  
...  

2008 ◽  
Vol 280 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Jing Xue ◽  
Gemei Liang ◽  
Neil Crickmore ◽  
Haitao Li ◽  
Kanglai He ◽  
...  

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 257-262
Author(s):  
Atanu Seni ◽  
Bhimasen Naik

Experiments were carried out to assess some insecticide modules against major insect pests of rice. Each module consists of a basal application of carbofuran 3G @ 1 kg a.i ha-1 at 20 DAT and Rynaxypyr 20 SC @ 30 g a.i ha-1 at 45 DAT except untreated control. All modules differ with each other only in third treatment which was applied in 65 DAT. The third treatment includes: Imidacloprid 17.8 SL @ 27 g a.i ha-1, Pymetrozine 50 WG @ 150 g a.i ha-1, Triflumezopyrim 106 SC @ 27 g a.i ha-1, Buprofezin 25 SC @ 250 g a.i ha-1; Glamore (Imidacloprid 40+Ethiprole 40% w/w) 80 WG @ 100 g a.i. ha-1, Thiacloprid 24 SC @ 60 g a.i ha-1, Azadirachtin 0.03 EC @ 8 g a.i ha-1, Dinotefuran 20 SG@ 40 g a.i ha-1 and untreated control. All the treated plots recorded significantly lower percent of dead heart, white ear- head caused by stem borer and silver shoot caused by gall midge. Module with Pymetrozine 50 WG @ 150 g a.i ha-1 treated plot recorded significantly higher per cent reduction of plant hoppers (>80% over untreated control) and produced higher grain yield (50.75 qha-1) than the other modules. Among the different treated modules the maximum number of spiders was found in Azadirachtin 0.03 EC @ 8 g a.i ha-1 treated module plot followed by other treatments.


2010 ◽  
Vol 134 (5) ◽  
pp. 429-438 ◽  
Author(s):  
L. Xu ◽  
Z. Wang ◽  
J. Zhang ◽  
K. He ◽  
N. Ferry ◽  
...  

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0161189 ◽  
Author(s):  
Yueqin Wang ◽  
Yidong Wang ◽  
Zhenying Wang ◽  
Alejandra Bravo ◽  
Mario Soberón ◽  
...  

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