scholarly journals DNA Barcoding of Two Narrow Endemic Plants; Astragalus Argaeus and Astragalus Stenosemioides from Mount Erciyes, Turkey

Author(s):  
handan Şapcı Selamoğlu

Abstract The genetic resources and biological diversity of countries are very important. Biodiversity and genetic resources should be protected, especially as endemic species. In this concept, DNA barcoding studies are an effective way to identify an unknown taxon and protected the biodiversity of a country. Astragalus argaeus and A. stenosemioides are narrow endemic species from Mt. Erciyes, Turkey. To determine its phylogenetic relationships and DNA barcoding, sequence data from the chloroplast DNA (matK region) were analyzed by parsimony, maximum likelihood, and Bayesian methods. In this study, A. argaeus, A. stenosemioides samples, and 23 sequences from GenBank, including a closely related species were performed. The phylogenetic analysis showed that the matK gene region could clearly distinguish A. argaeus and A. stenosemioides from its closely related species. DNA barcoding surveys can contribute to taxonomic and biodiversity research, various molecular ecology, and population genetics studies. Also, it is possible to determine the species by separating the matK DNA gene region, which is one of the molecular characters, and A. argaeus and A. stenosemioides have been successfully barcoded for the first time; therefore, it has been shown that this gene region can be used for barcoding.

Zootaxa ◽  
2007 ◽  
Vol 1562 (1) ◽  
pp. 43-53
Author(s):  
MOHSEN MOFIDI-NEYESTANAK ◽  
DONALD L.J. QUICKE

Eupholidoptera karatolosi Mofidi-Neyestanak & Quicke from Greece and E. mirzayani Mofidi-Neyestanak & Quicke from Iran, two new species of bushcrickets (Orthoptera, Tettigoniidae: Platycleidini), are described and distinguished from closely related species based on morphology. Species relationships within Eupholidoptera Maran are discussed and some species are given new assignments. A simplified illustrated identification key to the species of Eupholidoptera is provided to accommodate the new species. They are being described since they have been used to generate DNA sequence data that will be published elsewhere as part of a phylogenetic study of the tribe Platycleidini.


2016 ◽  
Vol 88 (3 suppl) ◽  
pp. 1809-1818
Author(s):  
LILIAN P.G. DA ROSA ◽  
JOSÉ F.A. BAUMGRATZ ◽  
SEBASTIÃO J. DA SILVA NETO

ABSTRACT Taxonomic and floristic studies in the state of Rio de Janeiro allowed the rediscovery of Miconia gigantea, an endemic species to the Atlantic Forest, until recently known only from the type specimen, collected over 100 years by A.F.M. Glaziou. We present an amended and detailed description of M. gigantea, providing characteristics of the flowers, fruits and seeds, in addition to illustrations, comments about taxonomic affinities with closely related species, the presently known distribution together with new occurrence records, and the conservation status.


2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Tai Wang ◽  
Yan-ping Zhang ◽  
Zhuo-yu Yang ◽  
Zhe Liu ◽  
Yan-yan Du

Abstract Background The northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs). Results After careful identification and comparison of the morphology and DNA barcoding of 1630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions. Conclusions The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.


Zootaxa ◽  
2010 ◽  
Vol 2694 (1) ◽  
pp. 1 ◽  
Author(s):  
TAHIRY RASOLONJATOVO HIOBIARILANTO ◽  
ROGER-DANIEL RANDRIANIAINA ◽  
JULIAN GLOS ◽  
AXEL STRAUß ◽  
MIGUEL VENCES

We provide morphological descriptions of the tadpoles of ten species of Malagasy treefrogs of the genus Boophis (family Mantellidae). Based on individuals determined by DNA barcoding, the larvae of eight species are described for the first time: B. anjanaharibeensis, B. axelmeyeri, B. elenae, B. englaenderi, B. luciae, B. rhodoscelis, B. roseipalmatus, and B. vittatus. For two additional species, B. andreonei and B. microtympanum, we provide descriptions from other localities than the previously known larvae. All tadpoles described herein are stream-adapted, exotrophic, and of a relatively generalized morphology. In general, we found a morphological similarity of larvae belonging to phenetic species groups of Boophis, but more detailed analyses showed several differences between taxa that are known to be sister species or closely related to each other: B. luciae has a much higher number of oral papillae than B. sibilans, B. roseipalmatus has a slightly lower number of papillae and possibly a stronger caudal musculature than B. madagascariensis, and B. elenae has a distinctly lower number of oral papillae and keratodonts in the first upper keratodont row than B. sandrae. This indicates that tadpole characters might, in some cases, provide good taxonomic characters in Boophis, a genus in which adults of closely related species are often morphologically extremely similar.


Zootaxa ◽  
2010 ◽  
Vol 2639 (1) ◽  
pp. 59 ◽  
Author(s):  
DAVID PORCO ◽  
LOUIS DEHARVENG

Deutonura gibbosa, a new species of the phlegraea group, is described. It is characterized by a large elongate uneven tubercle Di on Abd. V, with chaetae Di1 and Di2 shift backward. It has otherwise the same chaetotaxic pattern as the subspecies sylvatica of D. deficiens. Deutonura gibbosa sp. nov. is the most widespread Deutonura in the Alps and southern Jura. Its barcode sequence is provided and compared to those of four other Deutonura of the phlegraea group; it unambiguously differs from them, including from D. deficiens sylvatica, its closest relative according to current taxonomy. This is the most convincing example so far that barcode may help to discriminate taxa among closely related species in Collembola.


Phytotaxa ◽  
2016 ◽  
Vol 280 (2) ◽  
pp. 116 ◽  
Author(s):  
HUILI LI ◽  
XUELAN MA ◽  
PETER E. MORTIMER ◽  
SAMANTHA C. KARUNARATHNA ◽  
JIANCHU XU ◽  
...  

Four specimens of Phallus were collected during surveys in a Pinus armandii forest in Baoshan, Yunnan Province, China. Macro- and micro-characteristics, together with Internal Transcribed Spacer (ITS) sequence data, showed that the four specimens belong to a new species, here named Phallus haitangensis. The ITS phylogenetic analyses, morphological descriptions, color photographs, and line drawings are provided, and compared with closely related species in the genus.


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