scholarly journals QTL Mapping Integrated with BSA-Seq Analysis Identifies a Novel Gene Conferring Resistance to Brown Planthopper from Common Wild Rice (Oryza rufipogon Griff.)

2021 ◽  
Author(s):  
Xuan Wang ◽  
Yue Han ◽  
Yuexiong Zhang ◽  
Biao Deng ◽  
Biqiu Wu ◽  
...  
Keyword(s):  
Wild Rice ◽  
Major Gene ◽  
Brown Planthopper ◽  
Functional Characterization ◽  
Nilaparvata Lugens ◽  
Oryza Rufipogon ◽  
Rice Varieties ◽  
Novel Gene ◽  
Common Wild Rice ◽  
Nilaparvata Lugens Stål

Abstract The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive rice pests worldwide. GXU202 is a germplasm of common wild rice (Oryza rufipogon Griff. ) with high resistance to the BPH. In this study, the genetic analysis indicated that the BPH resistant phenotype of GXU202 is controlled by a major gene. Through the combination and comparison of QTL linkage and BSA-seq analyses, a novel gene locus BPH41 conferring BPH resistance was identified, which has been finely mapped to a 114-kb region delimited by D01031 and W1 on chromosome 4. The markers D01031 and D01045 showed high accuracy in predicting resistant phenotypes to BPH, suggesting their reliability for marker-assisted selection of BPH41 in breeding for BPH resistant rice varieties. The present identification of BPH41 will establish a foundation for further map-based cloning and functional characterization of the gene.

Feeding responses of the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), to resistant and susceptible host-plants

1989 ◽  
Vol 79 (2) ◽  
pp. 309-318 ◽  
Author(s):  
D. E. Padgham ◽  
S. Woodhead ◽  
H. R. Rapusas
Keyword(s):  
Resistance Genes ◽  
Host Plants ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Resistance Mechanisms ◽  
Population Development ◽  
Rice Varieties ◽  
Susceptible Host ◽  
Insect Activity ◽  
Nilaparvata Lugens Stål

AbstractThe feeding responses, growth and population development of Nilaparvata lugens (Stål) are quantified on a range of twelve susceptible or resistant host-plants. Paris of rice varieties carrying the Bph1, bph2 and Bph3 resistance genes are compared as hosts for N. lugens, and it is concluded that such notations do not adequately describe the diversity of plant resistance mechanisms. Evidence is presented for resistance mechanisms involving enhanced insect activity and gustatory responses to unacceptable phloem.

scholarly journals EFFECTS OF POPULATION DENSITY AND HOST AVAILABILITY ON THE MIGRATION PROCESS OF BROWN PLANTHOPPER FED USING SUSCEPTIBLE AND RESISTANT RICE VARIETIES (PENGARUH KEPADATAN POPULASI DAN KETERSEDIAAN PAKAN TERHADAP PROSES MIGRASI WERENG BATANG COKELAT PADA PADI VARIETAS RENTAN DAN TAHAN)

2017 ◽  
Vol 20 (2) ◽  
pp. 72
Author(s):  
Imam Habibi ◽  
Witjaksono Witjaksono ◽  
Arman Wijonarko
Keyword(s):  
Population Density ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Susceptible Variety ◽  
Resistant Variety ◽  
Rice Varieties ◽  
Host Availability ◽  
Important Pest ◽  
Nilaparvata Lugens Stål ◽  
Field Failure

Brown planthopper, Nilaparvata lugens Stal. (Hemiptera: Delphacidae), is an important pest of rice. This pest can cause hopperburn and field failure. This research aimed to determine the effects of population density and host availability on migration of N. lugens. The criteria used to justify the effects of host availability and population density on migration of N. lugens were based the hardness and tannin tests of the rice stems, fecundity of N. lugens, and the life cycle of N. lugens. The research was conducted under the temperature of 29.42°C with relative humidity of 61% and Light 12: Dark 12 times, using ten pairs of N. lugens brachypterous (F0 constant) and then was added with five male adults on fifth days after the first infestation (F0 changed). The varieties used were IR64, as a resistant variety, and Ketan Lusi, as a susceptible variety. The results showed that the adding of the macropterous males did not affect the number of macropterous, because of that has been preplanned by the F0. Therefore, the percentage of existing macropterous was 51−52%. Wereng Batang Cokelat (WBC) merupakan salah satu hama tanaman padi yang sangat penting. Kerusakan parah dapat menyebabkan hopperburn dan puso (gagal panen). Tujuan penelitian ini adalah mengetahui pengaruh kepadatan populasi dan tanaman inang sebagai tempat migrasi WBC. Parameter yang dikaji untuk mengetahui pengaruh kepadatan populasi WBC dan tanaman inang tempat migrasi WBC berdasarkan tingkat kekerasan dan kandungan tanin batang tanaman padi, fekunditas WBC, dan siklus hidup WBC. Penelitian ini dilakukan pada temperatur 29.42˚C dengan kelembapan relatif 61% dan durasi siang hari 12 jam: durasi malam hari 12 jam. Metode yang dilakukan adalah dengan menggunakan 10 pasang imago WBC brakhiptera (F0 konstan), kemudian dilakukan penambahan 5 ekor imago jantan pada hari kelima setelah infestasi awal (F0 diubah). Varietas padi yang digunakan yaitu padi varietas IR64 sebagai varietas tahan dan ketan Lusi sebagai varietas rentan. Hasil penelitian menunjukkan bahwa penambahan imago jantan makroptera tidak berpengaruh terhadap jumlah keturunan makroptera yang dihasilkan, karena imago (F0) telah merencanakan terlebih dahulu keturunan yang akan dihasilkan. Oleh karena itu, persentase terbentuk keturunan imago makroptera berkisar antara 51−52%. 

scholarly journals The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 498
Author(s):  
Cuong D. Nguyen ◽  
Holden Verdeprado ◽  
Demeter Zita ◽  
Sachiyo Sanada-Morimura ◽  
Masaya Matsumura ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Genes ◽  
Genetic Background ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Japonica Rice ◽  
Rice Varieties ◽  
Nilaparvata Lugens Stål

The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.

Application of SV markers developed from Dongxiang common wild rice in analysis of cultivated rice

2019 ◽  
Vol 17 (5) ◽  
pp. 448-451
Author(s):  
Fantao Zhang ◽  
Yuan Luo ◽  
Bin Ai ◽  
Yong Chen ◽  
Weidong Qi ◽  
...  
Keyword(s):  
Wild Rice ◽  
Oryza Rufipogon ◽  
Genomic Research ◽  
Cultivated Rice ◽  
Structural Variations ◽  
Rice Varieties ◽  
Common Wild Rice ◽  
Genome Wide ◽  
Rice Chromosomes ◽  
Molecular Marker Development

AbstractDongxiang common wild rice (Oryza rufipogon Griff., DXWR) is an important genetic resource for the improvement of cultivated rice. For the past three decades, great achievements have been made in the field of molecular marker development. Although structural variations (SVs) had been studied between DXWR and Nipponbare (Oryza sativa L. ssp. japonica), the development and application of SV markers in DXWR has not been reported. In this study, based on the genome-wide SV loci, we developed and synthesized a total of 195 SV markers that were evenly distributed across the 12 rice chromosomes. Then, these markers were tested for their stabilities and polymorphisms. Of these 195 markers, 147 (75.4%) were successfully amplified and displayed abundant polymorphisms between DXWR and Nipponbare. Meanwhile, through the genotyping of 20 rice varieties from 13 countries and areas, we concluded that these SV markers have a wide application prospect in the analysis of cultivated rice. Therefore, these molecular markers greatly enrich the number of markers available for DXWR, which will facilitate genomic research and molecular breeding for this important and endangered germplasm resource.

High Temperature Modifies Resistance Performances of Rice Varieties to Brown Planthopper, Nilaparvata lugens (Stål)

Rice Science ◽  
2010 ◽  
Vol 17 (4) ◽  
pp. 334-338 ◽  
Author(s):  
Bao-ju WANG ◽  
Hong-xing XU ◽  
Xu-song ZHENG ◽  
Qiang FU ◽  
Zhong-xian LU
Keyword(s):  
High Temperature ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Nilaparvata Lugens Stål

Dynamics of Yeast-like Symbiote and Its Relationship with the Virulence of Brown Planthopper, Nilaparvata lugens Stål, to Resistant Rice Varieties

2004 ◽  
Vol 7 (3) ◽  
pp. 317-323 ◽  
Author(s):  
Zhong-Xian Lu ◽  
Xiao-Ping Yu ◽  
Jian-Ming Chen ◽  
Xu-Song Zheng ◽  
Hong-Xing Xu ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Nilaparvata Lugens Stål
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