scholarly journals Micro-Injury Induces Hair Regeneration and Vitiligo Repigmentation Through Wnt/ β-Catenin Pathway

2021 ◽  
Author(s):  
Xiaofeng Han ◽  
Li Chang ◽  
Mao Lin ◽  
Dongwei Qi ◽  
Yuyi Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Skin Pigmentation ◽  
Treatment Method ◽  
Repair Process ◽  
Mechanism Analysis ◽  
Gene And Protein Expression ◽  
Hair Regeneration ◽  
Interfollicular Epidermis ◽  
And Migration

Abstract Background: Extrinsic injury can evoke intrinsic stimulation subquently initiate physiological repair process. Several kinds of injury have been studied to promote hair growth and skin pigmentation. In this study, we ask if proper injury could be employed to create local stimuli subsquently to induce hair regeneration and vitiligo repigmentation.Methods: We firstly manufactured a novel designed device to precisely control all micro-injury parameters. Then the most appropriate micro-injury extent was evaluated without over-damage to skin. The effects of micro-injury on hair regeneration and vitiligo repigmentation were examined by macroscopical observation, histological staining, gene and protein expression analysis.Results: We discover that proper micro-injury effectively induces hair regeneration by activating the hair follicle stem cell proliferation and migration downwards to hair matrix, finally shifting the hair follicle stage from telogen into anagen. On vitiligo model mice, micro-injury also induces the hair follicle melanocyte stem cell migrate upwards to interfollicular epidermis, activate and give rise to melanocytes to repopulate vitiligo lesion. Mechanism analysis indicates that the canonical Wnt/b-catenin pathway plays a key role in the micro-injury induced regeneration process. Conclusions: The present study demonstrates that micro-injury has great potential in inducing hair regeneration and vitiligo repigmentation, laid a foundation to develop micro-injury based treatment method in alopecia and vitiligo.

BMP signaling in the hair follicle stem cell niche regulates hair growth and skin pigmentation

2017 ◽  
Vol 86 (2) ◽  
pp. e60 ◽  
Author(s):  
Carlos Clavel ◽  
Delia Quek ◽  
Jamien Lim ◽  
Shuan Yong Teo
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Hair Growth ◽  
Stem Cell Niche ◽  
Skin Pigmentation ◽  
Bmp Signaling ◽  
Hair Follicle Stem Cell ◽  
Cell Niche

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

Hair follicle stem cell proliferation, Akt and Wnt signaling activation in TPA-induced hair regeneration

2017 ◽  
Vol 147 (6) ◽  
pp. 749-758 ◽  
Author(s):  
Weiming Qiu ◽  
Mingxing Lei ◽  
Ling Zhou ◽  
Xiufeng Bai ◽  
Xiangdong Lai ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Wnt Signaling ◽  
Hair Follicle ◽  
Stem Cell Proliferation ◽  
Hair Follicle Stem Cell ◽  
Hair Regeneration ◽  
Signaling Activation

scholarly journals The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 154
Author(s):  
Hanluo Li ◽  
Federica Francesca Masieri ◽  
Marie Schneider ◽  
Alexander Bartella ◽  
Sebastian Gaus ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Hair Follicle ◽  
Cell Populations ◽  
Stem Cell Markers ◽  
Outer Root Sheath ◽  
Root Sheath ◽  
Gene And Protein Expression ◽  
Stem Cell Populations

Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.

scholarly journals 687 BMP signaling in the hair follicle stem cell niche regulates hair growth and skin pigmentation

2016 ◽  
Vol 136 (5) ◽  
pp. S122
Author(s):  
J. Lim ◽  
D. Quek ◽  
J. Tan ◽  
C. Clavel
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Hair Growth ◽  
Stem Cell Niche ◽  
Skin Pigmentation ◽  
Bmp Signaling ◽  
Hair Follicle Stem Cell ◽  
Cell Niche

scholarly journals Functional complexity of hair follicle stem cell niche and therapeutic targeting of niche dysfunction for hair regeneration

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Chih-Lung Chen ◽  
Wen-Yen Huang ◽  
Eddy Hsi Chun Wang ◽  
Kang-Yu Tai ◽  
Sung-Jan Lin
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Stem Cell Niche ◽  
Therapeutic Targeting ◽  
Hair Follicle Stem Cell ◽  
Hair Regeneration ◽  
Functional Complexity ◽  
Cell Niche

Restoration of cartilage defects using a superparamagnetic iron oxide-labeled adipose-derived mesenchymal stem cell and TGF-β3-loaded bilayer PLGA construct

2020 ◽  
Vol 15 (6) ◽  
pp. 1735-1747
Author(s):  
Zhi-Gao Yang ◽  
Ruo-Fu Tang ◽  
Yi-Ying Qi ◽  
Wei-Ping Chen ◽  
Yan Xiong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Superparamagnetic Iron Oxide ◽  
Repair Process ◽  
Knee Joints ◽  
Blue Staining ◽  
Cartilage Defects ◽  
Gene And Protein Expression

Aim: We aimed to evaluate the capacity of the bilayer polylactic-co-glycolic acid (PLGA)/TGF-β3/adipose-derived mesenchymal stem cell (ADSC) construct used to repair cartilage defects and the role of ADSCs in the repair process in vivo. Materials & methods: Defects were created surgically on the femoropatellar groove of knee joints in 64 rabbits. All the rabbits were randomly divided into four groups: defect group, PLGA group, PLGA/TGF-β3 group and PLGA/TGF-β3/ADSC group. In vivo MRI and Prussian blue staining were applied. Quantitative real-time PCR and western blot methods were used to analyze the gene and protein expression. Results & conclusion: The result showed that TGF-β3 could effectively stimulate the expressions of aggrecan, collagen type II and SRY-related HMG box 9 ( SOX9). The bilayer PLGA/TGF-β3/ADSC construct showed a promising repair effect.

Sign in / Sign up

Export Citation Format

Share Document