scholarly journals Zincum Metallicum an Homeopathic Drug Alleviates Zn-Induced Toxic Effects, Promotes Plant Growth and Antioxidants Capacity in Lepidium Sativum

2021 ◽  
Author(s):  
Ghazwa BOUDALI ◽  
Tahar Ghnaya ◽  
Saoussen BEN-ABDALLAH ◽  
Abdala CHALAH ◽  
Abdelaziz SEBEI ◽  
...  
Keyword(s):  
Plant Growth ◽  
Lepidium Sativum ◽  
Carotenoid Content ◽  
Zn Toxicity ◽  
Oxidative Damages ◽  
Zn Uptake ◽  
Negative Effect ◽  
Homeopathic Drug ◽  
Leaves And Roots ◽  
Level 2

Abstract In this study, we investigated the effect of Zincum Metallicum (ZM) on zinc (Zn) toxicity in the plant species Lepidium sativum. We focused on growth, Zn uptake and numerous biochemical parameters. Seedlings were hydroponically subjected during 7 days to 0.05, 500, 1000, 1500 and 2000 µM Zn2+, in the absence or presence of 15ch or 9ch ZM. In the absence of ZM, Zn induced negative effect on growth especially at the dose of 2mM. Zn induced also chlorosis, reduced total chlorophyll and/or carotenoid content and increased the level of malondialdehyde (MDA). Under Zn-toxicity (500, 1000 and 1500 µM), the superoxide dismutase (SOD), catalase (CAT), gaiacol peroxidase (GPX) and glutathione reductase (GR) activities were increased and/or unaltered, while at 2000 µM Zn affected the activity of these enzymes. At the highest Zn level (2 mM), proline and total polyphenols and flavonoids contents were markedly induced in leaves and roots of L. sativum. Additionally, the supply of ZM in the nutrient medium considerably ameliorated the plant growth, photosynthetic pigments and the studied non-enzymatic antioxidant molecules and enzymatic activities against Zn induced-oxidative stress. Our data suggest that the potential homeopathy properties of ZM may be efficiently involved in the restriction of Zn-induced oxidative damages, by lowering Zn accumulation and translocation in the leaves and roots of Lepidium sativum.

Quality and Intensity of Light in the In Vitro Development of Microstumps of Eucalyptus urophylla in a Photoautotrophic System

2020 ◽  
Vol 66 (6) ◽  
pp. 754-760 ◽  
Author(s):  
Natane A Miranda ◽  
Aloisio Xavier ◽  
Wagner C Otoni ◽  
Ricardo Gallo ◽  
Kellen C Gatti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Light Quality ◽  
Stomatal Density ◽  
Carotenoid Content ◽  
Fluorescent Light ◽  
Eucalyptus Urophylla ◽  
Light Emitting ◽  
Fluorescent Lamps ◽  
Number Of Shoots

Abstract The quality and quantity of light are important factors in controlling in vitro plant growth in photoautotrophic systems. The aim of this study was to evaluate the influence of light quality (fluorescent, white, red, blue, red/blue, and distant red) on microstumps of a Eucalyptus urophylla clone in an in vitro photoautotrophic system, as well as the intensity of fluorescent light (60, 85, 100, and 140 μmol m–2 s–1) in the growth and production of microcutting. The number of shoots and microcutting, the size of the largest shoot, the stomatal density, chlorophyll, and carotenoid content were analyzed. Light quality altered plant growth, and fluorescent light intensity did not affect the microstumps’ production during the evaluation period. In white light-emitting diode (LED) light, there was higher production of carotenoids, with a lower initial production of microcuttings. A smaller number of shoots were obtained in blue LED. In general, the different qualities and light intensities tested allowed for the growth of the Eucalyptus urophylla clone grown in vitro, making it possible to obtain microcuttings under photoautotrophic cultivation. Study Implications In vitro propagation is a stressful process for plants and has limitations for commercial-scale Eucalyptus production. Fluorescent lamps, closed containers, and high sucrose concentrations are traditionally used. To reduce costs and improve production, the use of efficient light sources and photoautotrophic cultivation systems become alternatives. This study investigated the influence of light on the in vitro growth of a Eucalyptus clone in a photoautotrophic system. The quality was more important than the intensity of light. Foresters will be able to indicate the use of LEDs (light-emitting diodes) as a replacement for fluorescent lamps. This approach is useful in enhancing micropropagation techniques.

scholarly journals The effect of zinc supply and succinate treatment on plant growth and mineral uptake in pea plant

2002 ◽  
Vol 14 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Zlatimira Stoyanova ◽  
Snejana Doncheva
Keyword(s):  
Pisum Sativum ◽  
Plant Growth ◽  
Organic Anion ◽  
Mineral Uptake ◽  
Stress Indicators ◽  
Zn Toxicity ◽  
Pisum Sativum L ◽  
Ameliorative Effect ◽  
Stems And Leaves ◽  
As Stress

The influence of succinate treatment on Zn toxicity was investigated using plant growth and mineral uptake as stress indicators. Pea plants (Pisum sativum L., cv. Citrine) were treated with various Zn concentrations (0.67 to 700 muM Zn) in the presence and absence of 0.2 mM Na-succinate. Plants pre-treated with succinate and then exposed to Zn exhibited higher dry root, stem and leaf weight than the plants treated with Zn alone. An increase in Zn supply resulted in a decrease in the concentrations of Ca, Mg, P in the roots and an increase of Ca and N levels in the stems and leaves. The amount of Zn in the roots, stems and leaves increased with greater Zn rates. The succinate treatment increased P in the roots but did not affect the Ca, N and Mg contents in Zn-treated plants. Most of the Zn taken up was retained in the roots after succinate treatment. The ameliorative effect of succinate on plant growth could be due to a lower Zn translocation in the leaves and stems and increased Zn accumulation in the roots. Lower Zn translocation in aboveground parts seemed to result from Zn complexing by organic anion in the roots. This probably caused less Zn transport to the stems and leaves and suggested that succinate has potential for complexing with Zn and may play a role in tolerance to high Zn levels.

Two bacterial strains isolated from a Zn-polluted soil enhance plant growth and mycorrhizal efficiency under Zn-toxicity

Chemosphere ◽  
2006 ◽  
Vol 62 (9) ◽  
pp. 1523-1533 ◽  
Author(s):  
A. Vivas ◽  
B. Biró ◽  
J.M. Ruíz-Lozano ◽  
J.M. Barea ◽  
R. Azcón
Keyword(s):  
Plant Growth ◽  
Polluted Soil ◽  
Bacterial Strains ◽  
Zn Toxicity

scholarly journals ВМІСТ ФОТОСИНТЕТИЧНИХ ПІГМЕНТІВ У ЛИСТКАХ ГРЕЧКИ ЗА ДІЇ БІОЛОГІЧНИХ ПРЕПАРАТІВ

2021 ◽  
Vol 81 (1-2) ◽  
pp. 83-89
Author(s):  
V. P. Karpenko ◽  
I. I. Mostoviak ◽  
A. A. Datsenko ◽  
R. M. Prytuliak ◽  
O. I. Zabolotnyi
Keyword(s):  
Plant Growth ◽  
Chlorophyll A ◽  
Plant Development ◽  
Seed Treatment ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Biochemical Processes ◽  
Chlorophyll A And B ◽  
Microbial Preparation ◽  
Physiological And Biochemical

The article presents the results of a field experiment to study the effects of different rates of the microbial preparation Diazobacterin under different methods of using the plant growth regulator Radostim (seed treatment before sowing and spraying crops) on the content of chlorophyll a and b in buckwheat leaves of Yelena variety, their sum and carotenoids. Determination of chlorophyll a and b, their sum and carotenoids was performed in selected samples of buckwheat leaves in the field conditions, in the phases of stem branching and the beginning of flowering according to the method described by V.F. Gavrylenko and T.V. Zhyhalova using a spectrophotometer. The pigment concentration was calculated from Holm-Wettstein's equations for 100 % acetone. It was found that the content of photosynthetic pigments in buckwheat leaves is conditioned by the weather, rates and methods of application of the studied microbial preparations and phases of plant development. In particular, the analysis of chlorophyll and carotenoid content in the early flowering phase of buckwheat plants showed a significant increase compared to the phase of stem branching, which may be due to increased physiological and biochemical processes in plants, against the background of improving mineral nutrition, growth and plant development. Thus, with the multiple use of the microbial preparation Diazobacterin in the rates of 150, 175 and 200 ml and the stimulator of plant growth Radostim in the rate of 250 ml / t for seed treatment before sowing, the content of chlorophylls a and b, their sum and carotenoids with increasing application of Diazobacterin increased and the excess relative to control ranged within: 29–31 % for chlorophyll a; 10–11 % for chlorophyll b; 24–26 % for their sum and 23–24 % for carotenoids. At the same time, with a complex application for pre-sowing seed treatment of a mixture of the microbial preparation Diazobacterin in the rate of 200 ml per hectare of seeds with the stimulator of plant growth Radostym in the rate of 250 ml / t followed by spraying on this background crops the stimulator of plant growth Radostim in the rate of 50 ml / ha buckwheat leaves form the highest content of chlorophyll a in comparison with the control (40 %); chlorophyll b (15 %); the sum of chlorophyll a + b (33 %) and carotenoids (29 %). These data indicate the creation of more favorable conditions for physiological and biochemical processes in plants, including photosynthesis, with the direct positive effect of which, functionally active pigment complex of the leaf apparatus of buckwheat is formed.

scholarly journals Enhance Growth and Flower Quality of Chrysanthemum indicum L. with Application of Plant Growth Retardants

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 532
Author(s):  
Endre Kentelky ◽  
Zsolt Szekely-Varga ◽  
János Bálint ◽  
Adalbert Balog
Keyword(s):  
Plant Growth ◽  
Ornamental Plants ◽  
Growth Inhibitors ◽  
Growth Retardants ◽  
Chrysanthemum Indicum ◽  
Negative Effect ◽  
Plant Growth Retardants ◽  
Flower Quality ◽  
Plant Growth Inhibitors

Ornamental plants are cultivated worldwide. Chrysanthemum is classified as one of the most important cut and potted flowers in most of the countries. The consumer’s expectation is to find small–compact, and full of inflorescences plants. To meet these demands, growers are tending to use plant growth retardants. Three Chrysanthemum indicum L. varieties (‘Smola White’, ‘Arber’ and ‘Vienna White’) were assessed by using four plant growth regulators (PP–Bumper 250 EC; CC–Stabilan SL; MP–Medax Top SC; and PD–Toprex SC). Results indicate that treated plants show significant decrease in the assessed parameters, although in some cases growth could be a variety–dependent factor. It can also be concluded that retardants inhibit chrysanthemum growth. PD treatments greatly inhibited the growth of the plant, and also had a negative effect on inflorescences. In conclusion, the present work strengthens the possibility of using retardants as plant growth inhibitors in Chrysanthemum cultivation.

scholarly journals Arbuscular Mycorrhizal Fungi Alleviate Arsenic Toxicity in Sophora Viciifolia Hance. by Improving The Growth, Photosynthesis, Reactive Oxygen Species and Gene Expression of Phytochelatin Synthase

2021 ◽  
Author(s):  
QiaoMing Zhang ◽  
Minggui Gong ◽  
Shanshan Xu ◽  
Angran Zhang ◽  
Jiangfeng Yuan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plant Growth ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Photochemical Quenching ◽  
Antioxidant Enzyme Activities ◽  
Oxygen Species ◽  
Leaves And Roots ◽  
As Stress ◽  
As Toxicity

Abstract Arbuscular mycorrhizal fungi (AMF) can protect host plants against arsenic (As) toxicity. However, knowledge on the response of woody leguminous under As stress is limited so far. In this study, Sophora viciifolia seedlings were inoculated with/without AMF Rhizophagus intraradices, and S. viciifolia were grown in three levels (0, 50, and 100 mg As kg−1 soil) of As-polluted soil though the potted experiments. The objective of this study was to investigate the influences of AMF symbiosis on woody leguminous under As stress. Some physiological and biochemical parameters of S. viciifolia, which included the plant growth, photosynthesis, oxidative damage, antioxidant enzyme activities and gene expression of phytochelatins (PCs), were analyzed. The results showed that As toxicity in soils inhibited the AM colonization rate, plant growth, photosynthesis, increased the oxidative damage and antioxidant enzyme activities, and up-regulated the gene expression of SvPCS1 in the leaves and roots of S. viciifolia seedlings. However, compared with non-inoculated S. viciifolia at the same As level, R. intraradices-inoculated S. viciifolia had higher shoot and root dry weight, plant height, root length, photosynthetic rate (Pn), stomatal conductance (gs), transpiration rate (E), maximal photochemical efficiency of PSII photochemistry (Fv/Fm), actual quantum yield (ΦPSII), and photochemical quenching values (qP), as well as lower intercellular CO2 concentration (Ci) and non-photochemical quenching values (NPQ). R. intraradices inoculation inhibited the malondialdehyde (MDA), H2O2, and O2•– concentrations, but improved the activities of antioxidative enzymes (SOD, POD, and CAT) in S. viciifolia leaves and roots. The gene expression of SvPCS1 in the leaves and roots was obviously up-regulated by R. intraradices inoculation. These results demonstrated that R. intraradices inoculation enhanced the As tolerance of S. viciifolia seedlings, owing to the beneficial effects of AMF symbiosis on improving the plant growth, gas exchange, chlorophyll fluorescence, antioxidant enzymes, reactive oxygen species and gene expression of SvPCS1 in S. viciifolia seedlings. R. intraradices is possible to get involved in the defence response of S. viciifolia seedlings against. As toxicity stress. This investigation got more profound insights into As tolerance mechanisms of woody leguminous associated with AMF symbiosis.

scholarly journals Efektivitas Formulasi Spora Bacillus subtilis B12 sebagai Agen Pengendali Hayati Penyakit Hawar Daun Bakteri pada Tanaman Padi

2015 ◽  
Vol 34 (1) ◽  
pp. 21 ◽  
Author(s):  
Wartono Wartono ◽  
Giyanto Giyanto ◽  
Kikin H. Mutaqin
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Foliar Spray ◽  
Leaf Blight ◽  
Plant Diseases ◽  
Bacterial Leaf Blight ◽  
Growth Promoter ◽  
Negative Effect ◽  
Plant Growth Promoting ◽  
Blight Disease

<p>Bacterial leaf blight control in rice (Oryza sativa Lin.) using bactericide is prohibitive, due to its high cost and its negative effect on the environment. Biocontrol when avaible, therefore is the best alternative solution. Bacillus subtilis is a perspective of biocontrol agent to control several plant diseases, because of its ability to produce antimicrobial and produce plant growth promoting substances. This research was aimed to examine the effectiveness of B. subtilis spore formulation by way of seed treatments and foliar sprays, using different frequencies and concentrations, to control bacterial leaf blight disease (BLB) on rice, caused by Xanthomonas oryzae pv. oryzae, and to evaluate its function as plant growth promoter. The experiments were conducted at greenhouse and in the field using factorial design. At the greenhouse experiment, seed treatment and foliar spray, using concentration of 2% produced better result in controlling BLB, and better result on promoting rice plant growth. In the field experiment, application at 2 week interval showed better effect on suppressing the the disease and on increasing yield. Applications of the formulation of B. subtilis B12 spore reduced BLB disease by 21% and potentially increased yield up to 50%.</p>

scholarly journals Gly Betaine Surpasses Melatonin to Improve Salt Tolerance in Dalbergia odorifera

2021 ◽  
Vol 12 ◽  
Author(s):  
El Hadji Malick Cisse ◽  
Ling-Feng Miao ◽  
Fan Yang ◽  
Jin-Fu Huang ◽  
Da-Dong Li ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Crop Production ◽  
Growth Parameters ◽  
Soluble Sugar ◽  
Carotenoid Content ◽  
Guaiacol Peroxidase ◽  
Agricultural Crop ◽  
Salt Stress Condition ◽  
Dalbergia Odorifera

Salinity is one of the most serious factors limiting plant growth which can provoke significant losses in agricultural crop production, particularly in arid and semi-arid areas. This study aimed to investigate whether melatonin (MT; 0.05 and 0.1 mM), which has pleiotropic roles, has a better effect than glycine betaine (GB; 10 and 50 mM) on providing salt tolerance in a woody plant Dalbergia odorifera T. Chen. Also, the alternative oxidase activity (AOX) in plant subjected to MT or GB under salinity (150 and 250 mM) was evaluated given that the effect of exogenous MT or GB on AOX has not been reported yet. The results showed that the exogenous application of GB on the seedlings of D. odorifera increased the plant growth parameters, relative water content, total of chlorophyll content, and carotenoid content compared with well-watered and MT treatments. Under severe salinity, the seedlings subjected to GB showed, a significant enhancement in water use efficiency, transpiration, and net photosynthetic rate regardless to MT-treated seedlings. The levels of proline and soluble sugar in the seedlings treated with MT or GB decreased significantly under mild and severe salinity correlated with those in salt-stressed seedlings. Furthermore, GB-treated plants exhibited a significant inhibition of malondialdehyde content compared with MT-treated plants. The concentration of thiols and phenolic compounds were significantly enhanced in the leaves of seedlings treated with MT compared with those treated with GB. Under salt stress condition, GB scavenged significantly higher levels of hydrogen peroxide than MT; while under severe salinity, plants subjected to MT showed better scavenging ability for hydroxyl radicals compared with GB-treated seedlings. The results demonstrated also an enhancement of the levels of superoxide dismutase (SOD), guaiacol peroxidase, and AOX activities in seedlings treated with GB or MT compared with salt-stressed plants. The catalase activity (CAT) was increased by 0.05 mM MT and 0.1 mM GB under mild salinity. Meanwhile, the AOX activity under severe salinity was enhanced only by GB 50 mM. The findings of this study suggested that GB-treated seedlings possessed a better salt tolerance in comparison with MT-treated seedlings.

Sign in / Sign up

Export Citation Format

Share Document