scholarly journals The Frequency of Class1 and 2 and 3 Integrons in Vancomycin-resistant and Aminoglycoside Resistance Enterococcus Strains Isolated From Burn Patients in Southwest Iran

2022 ◽  
Keyword(s):  
Full Text ◽  
Burn Patients ◽  
Aminoglycoside Resistance ◽  
Vancomycin Resistant ◽  
Southwest Iran

Abstract The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.

scholarly journals The Frequency of Class1 and 2 and 3 Integrons in Vancomycin-resistant and Aminoglycoside Resistance Enterococcus Strains Isolated From Burn Patients in Southwest Iran

2021 ◽  
Author(s):  
Effat Abbasi Montazeri ◽  
Azar Dokht Khosravi ◽  
Khadijah Ahmadi ◽  
Maryam Afzali ◽  
Aram Asareh zadegan dezfuli
Keyword(s):  
Resistance Pattern ◽  
Burn Patients ◽  
Vancomycin Resistant Enterococcus ◽  
Enterococcus Species ◽  
Aminoglycoside Resistance ◽  
Vancomycin Resistant ◽  
Southwest Iran

Abstract Background: This study aimed to detect the frequency of class1 and 2 and 3 integrons in vancomycin-resistant enterococcus (VRE) and aminoglycoside resistance strains isolated from burn patients in Taleghani burn hospital, AhvazResults: Out of the 129 Enterococcus species 42% were E. faecalis and 57% isolated were E. faecium. 34% were resistant to vancomycin. In the E. faecium isolates, 21% were XDR and 67%) were MDR. In the E. faecalis isolates, 74% were MDR isolates. Among 129 Enterococci spp. Isolates 45% E. faecium and 20% E. faecalis isolates were positive for the presence of vanA genes. In addition, 20% E. faecium and 0.04% E. faecalis isolates were positive for the presence of vanB genes. Thirty-one E Enterococci spp including E. faecium 79% and E. faecium 58% isolates carried int 1. In VRE E. faecalis 57.1% isolates positive for the presence of int 1and 57.1% isolates positive for the presence of int 1. Conclusion: we showed a detailed resistance pattern of clinically isolated Enterococci species to common antibiotics. Accordingly, the results confirmed the association between simultaneous resistance to vancomycin. These results can guide antibiotic prescriptions against Enterococci infections.

PREVALENCE OF HIGH LEVEL AMINOGLYCOSIDE RESISTANCE AND VANCOMYCIN RESISTANT ENTEROCOCCI IN ENTEROCOCCAL ISOLATES IN A TERTIARY CARE HOSPITAL

2021 ◽  
pp. 3-5
Author(s):  
Sunita Agarwal ◽  
Nazneen Pathan ◽  
Shivra Batra ◽  
Rajni Sharma
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Tertiary Care ◽  
Automated System ◽  
Care Hospital ◽  
Enterococcus Species ◽  
Aminoglycoside Resistance ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
High Level

Introduction: The emergence of High Level Aminoglycoside Resistance (Resistant to Gentamycin and Streptomycin) and Vancomycin Resistant Enterococci among Indoor and Intensive Care Unit admitted patient presents a serious challenge for clinicians. Objective: To determine Enterococcal burden in blood and urine specimens and to detect the prevalence of High Level Aminoglycoside Resistance and Vancomycin Resistant Enterococci. Material & Methods: One hundred ten Enterococci were isolated from blood and urine samples and processed according to standard laboratory protocol. Species identication and sensitivity was done using the VITEK 2 automated system (Biomerieux France) with the cards GPID and AST 67 respectively. Results: Out of 110 Enterococci isolates, 36 were from blood and 74 from urine were detected. Different Species isolated were Enterococcal faecium (59%), Enterococcal faecalis (34%), Enterococcal rafnosus (2.7%), Enterococcal gallinarum (1.8%), Enterococcal casseliavus (0.9%) and Enterococcal duran (0.9%).Out of 36 blood isolates, 14 (38%) were found to be both High Level Gentamycin Resistant (HLGR) & High Level Streptomycin Resistant (HLSR), 10 (27%) were only HLGR and 8 (22%) were only HLSR. 20 strain (55%) of Enterococcus species isolated in blood were VRE. All VRE strains were found to be resistant to both aminoglycosides ( HLAR).Among the 74 urinary isolates, 24 (34%) were found to be both HLGR & HLSR, only HLGR was observed in 20 (27%) and HLSR was observed in 11 (14%) isolates. 24 strains (34%) of Enterococcus species were found to be vancomycin resistant in urine. 23 strains out of 24 were resistant to high level of aminoglycosides. Conclusion: The prevalence of HLAR and VRE is very high among Enterococcus specimens from indoor/ intensive care unit patients. Early species identication and antibiotic sensitivity result can help in better clinical outcome.

scholarly journals Detection of various virulence factors in high level aminoglycoside resistance and vancomycin resistant enterococci isolates of uropathogenic Enterococci

2019 ◽  
Vol 7 (5) ◽  
pp. 1740
Author(s):  
Harshad Singh Naruka ◽  
Anita E. Chand ◽  
Pradhuman Singh Chauhan ◽  
Danish Mukhtar
Keyword(s):  
Virulence Factors ◽  
Common Factor ◽  
Opportunistic Pathogen ◽  
Government Medical College ◽  
Aminoglycoside Resistance ◽  
Medical College ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Study Association ◽  
High Level

Background: Enterococci are common commensal organism of enteric tract and act as opportunistic pathogen and may cause infection in community as well as in hospitalised individuals. In present study association of several types of virulence factors like haemolysin, gelatinase and biofilm formation have been studied among HLAR and Vancomycin resistant Enterococci (VRE) isolates of enterococci among UTI patients.Methods: The samples were collected from all hospitalized and OPD patients of MBS Hospital, JK Lone Hospital and NMC Hospital. Government Medical College, Kota, Rajasthan, India. A total of 360 isolates of enterococcus were collected during the period of 2 years from April 2016 to April 2018 in microbiology laboratory, Department of Microbiology, Government Medical College, Kota, Rajasthan, India. All virulence factors were detected by phenotypic methods and MIC values were detected for high level gentamicin and vancomycin.Results: Among all enterococcal isolates most common factor was biofilm production 191 (53.05%) followed by haemolysin 131 (36.38%) and gelatinase production 72 (20%). Total resistant (MIC> 500 µg/ml) isolates for gentamicin was 194 (89.4%). In agar dilution 14 (11.2%) isolates were found sensitive, 61 (48.8%) isolates were found intermediate and 50 (40%) isolates were found to be resistant for vancomycin. HLAR and VRE was maximum associated with haemolysin + bio-film followed by gelatinase+biofilm, haemolysin+gelatinase+bio- film and least with haemolysin + gelatinase.Conclusions: In present study enterococcus show significant production of biofilm and other virulence factors. With production of biofilm they become more resistant to routinely used concentration of antibiotics posing threat for treatment failure. A continuous monitoring is needed particularly for resistance to aminoglycoside and vancomycin to stop their institutional spread. Judicial use of antibiotics should be encouraged both in community as well as in institutions.

scholarly journals A Comparison of High-Level Aminoglycoside Resistance in Vancomycin-Sensitive and Vancomycin-Resistant Enterococcus Species

2002 ◽  
Vol 30 (5) ◽  
pp. 529-534 ◽  
Author(s):  
H Yazgi ◽  
M Ertek ◽  
S Erol ◽  
A Ayyildiz
Keyword(s):  
Brain Heart Infusion ◽  
Bactericidal Effect ◽  
Vancomycin Resistance ◽  
Glycopeptide Antibiotics ◽  
Aminoglycoside Resistance ◽  
Significant Difference ◽  
Vancomycin Resistant ◽  
High Level ◽  
Infusion Agar ◽  
Level Resistance

The aim of this study was to investigate whether there was a significant difference in high-level aminoglycoside resistance (HLAR) between vancomycin-sensitive enterococci (VSE) and vancomycin-resistant enterococci (VRE). Vancomycin resistance was determined in 116 Enterococcus isolates using brain-heart infusion agar containing 6 μg/ml vancomycin. HLAR was determined by both standard agar screening and disk diffusion methods. Streptomycin and gentamicin were used as predictors of HLAR. Vancomycin resistance and HLAR were found in 17 (14.7%) and 41 (35.3%) of the Enterococcus strains, respectively. HLAR was found in 11 of 17 VRE and 30 of 98 VSE strains. HLAR in VRE strains was significantly higher than in VSE. More enterococcal strains were found to be resistant to both gentamicin and streptomycin (29) than to gentamicin (one) or streptomycin (11) alone. The HLAR rate in VRE was two-fold higher than in VSE. The synergistic bactericidal effect of aminoglycosides and β-lactam or glycopeptide antibiotics is lost if there is high-level resistance to aminoglycosides.

scholarly journals Frequency of vancomycin-resistant Enterococci isolated from blood cultures from 2008 to 2010

2011 ◽  
Vol 64 (9-10) ◽  
pp. 481-485 ◽  
Author(s):  
Mira Mihajlovic-Ukropina ◽  
Deana Medic ◽  
Zora Jelesic ◽  
Vera Gusman ◽  
Biljana Milosavljevic
Keyword(s):  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Blood Cultures ◽  
Hospitalized Patients ◽  
Diffusion Method ◽  
Aminoglycoside Resistance ◽  
Vancomycin Resistant Enterococci ◽  
Enterococcus Spp ◽  
Vancomycin Resistant ◽  
High Level

Introduction. Enterococci are important hospital-acquired pathogens. The most commonly isolated species of the genus, Enterococcus faecalis and Enterococcus faecium are the third to fourth-most prevalent nosocomial pathogens worldwide. The aim of this study was to determine the frequency of resistance to vancomycin and other antimicrobial agents of Enterococcus spp strains isolated from blood cultures of hospitalized patients. Material and methods. During the three-year period, from 2008 to 2010, 132 strains of Enterococcus spp isolated from blood cultures of hospitalized patients were tested for their susceptibility to ampicillin, vancomycin, gentamycin (high-level resistance), erythromycin, chloramphenicol, teicoplanin, ciprofloxacin by disc diffusion method according to the Clinical and Laboratory Standards Institute recommendations. Susceptibility of vancomycin resistant E. faecium to the same antibiotics and to linezolid, quinopristin/dalfopristin and tigecyclin was determined using VITEK system. Results and discussion. Resistance to vancomycin was detected in 21 (15.9%) Enterococcus spp strains. The percentage of resistance to other antimicrobial agents varied from 23.1% for chloramphenicol to 81.3% for ciproflxacin. All vancomycin resistant enterococci were identified as E. faecium and belonged to phenotype VanA. The resistance to other antibiotics was very high, except for linezolid and quinopristin/dalfopristin (4.7%). The high-level aminoglycoside resistance was 87.6% for gentamycin and 95.2% for streptomycin. All isolates were resistant to ampicillin, teicoplanin and ciprofloxacin. Conclusion. The detected high frequency of multidrug-resistant isolates among vancomycin resistant enterococci is of great importance and suggests the need for further monitoring of susceptibility in order to take adequate measures to prevent and control spreading of resistant strains.

Resistance pattern of enterococcus for high level aminoglycosides and vancomycin in blood culture of admitted patients

2016 ◽  
Vol 5 (01) ◽  
pp. 4756
Author(s):  
Shadma Yaqoob
Keyword(s):  
Blood Culture ◽  
Multidrug Resistant ◽  
Screening Tests ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Aminoglycoside Resistance ◽  
Vancomycin Resistant ◽  
Blood Specimens ◽  
High Level ◽  
Clinical Conditions

Enterococci have emerged as important nosocomial pathogens from a variety of clinical conditions and the major reason for this is the trend of increasing antimicrobial resistance and enterococcal bacteraemia results in a high mortality. The present study was undertaken to determine the occurrence, species prevalence, antibacterial resistance, with a special reference to vancomycin and high level aminoglycoside resistance. Material and methods: The study was conducted on blood culture isolates. The antibiotic susceptibility of isolates by the Kirby Bauer disc diffusion method was done according to the CLSI guidelines. Screening tests for high level aminoglycosides and vancomycin and minimum inhibitory concentration (MIC) tests for vancomycin was done. The blood specimens were cultured and suspected growths were identified to species level and found to consist mostly of E. fecalis (70%). VRE accounted for 2 (91%) isolates and high level aminoglycoside resistance was seen in 47.82 and 60.86 isolates. The E. faecium isolates were more drug resistant than the E. faecalis isolates. Linezolid and Teicoplanin showed good anti-enterococcal activity. This study shows an emergence of Vancomycin resistant enterococcus (VRE) along with increased rate of multidrug-resistant enterococci. Regular surveillance of antimicrobial susceptibilities and treatment of enterococcal infections should be done effectively to limit the spread of multidrug resistance.

scholarly journals Pseudomonas aeruginosa infection in burn patients in Sulaimaniyah, Iraq: risk factors and antibiotic resistance rates

2014 ◽  
Vol 8 (11) ◽  
pp. 1498-1502 ◽  
Author(s):  
Nasih Othman ◽  
Muhammed Babakir-Mina ◽  
Chia Kamil Noori ◽  
Parihan Yahya Rashid
Keyword(s):  
Risk Factors ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Full Text ◽  
Burn Patients ◽  
Pseudomonas Aeruginosa Infection ◽  
Resistance Rates

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scholarly journals Study of Biofilm Formation and Antibiotic Resistant Profile in Multidrug Resistant and Extensive Drug Resistance Pseudomonas Aeruginosa Isolated from Burn Wound Infections in Southwest Iran

2021 ◽  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Full Text ◽  
Biofilm Formation ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Burn Wound ◽  
Antibiotic Resistant ◽  
Extensive Drug Resistance ◽  
Southwest Iran

Abstract The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.

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