scholarly journals A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics

2021 ◽  
Author(s):  
Raymond Thomas ◽  
Charles Manful ◽  
Thu Pham
Keyword(s):  
Species Composition ◽  
Molecular Species ◽  
Analytical Method ◽  
Biological Samples ◽  
Reversed Phase ◽  
Industrial Applications ◽  
Molecular Species Composition ◽  
High Throughput Analysis ◽  
Flame Ionization ◽  
Phase Liquid

Abstract IntroductionMonoacetyldiglycerides (MAcDG), are acetylated triglycerides (TG) and an emerging class of bioactive or functional lipid with promising nutritional, medical, and industrial applications. A major challenge exists when analyzing MAcDG from other subclasses of TG in biological matrices, limiting knowledge on their applications and metabolism. Objective and MethodsHere in we demonstrate a multimodal analytical method for resolution, identification and quantitation of MAcDG in biological samples based on thin layer chromatography-flame ionization detection complimentary with C30-reversed phase liquid chromatography-high resolution accurate mass tandem mass spectrometry. We further apply this method to determine the MAcDG molecular species composition and quantity in E. solidaginis larvae. Results and ConclusionThese findings suggest that the proposed analytical method could simultaneously provide a fast, accurate, sensitive, high throughput analysis of MAcDG from other TG subclasses, including the fatty acids, isomers and molecular species composition. We believe this method will allow for MAcDG to be included during routine lipidomics analysis of biological samples and will have broad interests and applications in the scientific community in areas such as nutrition, climate change, medicine and biofuel innovations.

Molecular species composition of glycolipids from Sprirulina platensis

2002 ◽  
Vol 77 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Changhu Xue ◽  
Yaqin Hu ◽  
Hiroaki Saito ◽  
Zhaohui Zhang ◽  
Zhaojie Li ◽  
...  
Keyword(s):  
Species Composition ◽  
Molecular Species ◽  
Molecular Species Composition

scholarly journals The contributions of biosynthesis and acyl chain remodelling to the molecular species profile of phosphatidylcholine in yeast

2005 ◽  
Vol 33 (5) ◽  
pp. 1146-1149 ◽  
Author(s):  
H.A. Boumann ◽  
A.I.P.M. de Kroon
Keyword(s):  
Stable Isotope ◽  
Species Composition ◽  
Molecular Species ◽  
Acyl Chain ◽  
Membrane Lipid ◽  
Molecular Species Composition ◽  
Stable Isotope Labelling ◽  
Membrane Function ◽  
Isotope Labelling ◽  
Acyl Chains

Phosphatidylcholine (PC) is a very abundant membrane lipid in most eukaryotes, including yeast. The molecular species profile of PC, i.e. the ensemble of PC molecules with acyl chains differing in number of carbon atoms and double bonds, is important for membrane function. Pathways of PC synthesis and turnover maintain PC homoeostasis and determine the molecular species profile of PC. Studies addressing the processes involved in establishing the molecular species composition of PC in yeast using stable isotope labelling combined with detection by MS are reviewed.

Targeted analysis of phenolic compounds by LC-MS v1

2021 ◽  
Author(s):  
Bashar Amer ◽  
Ramu Kakumanu ◽  
yangtian not provided ◽  
Aymerick Eudes ◽  
Edward EK Baidoo
Keyword(s):  
Phenolic Compounds ◽  
Plant Biomass ◽  
Reversed Phase ◽  
Industrial Applications ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reversed Phase Liquid Chromatography ◽  
Sample Matrix ◽  
Targeted Analysis ◽  
Challenges And Opportunities ◽  
Phase Liquid

Cell-wall-bound (CWB) aromatics such as ferulate and p-coumarate play important physiological roles in plant development and response to stresses. Their presence also poses some challenges and opportunities during processing of plant biomass in various agro-industrial applications. To this end, we have developed a robust high-throughput reversed-phase liquid chromatography mass spectrometry method for quantifying CWB phenolic compounds. The method showed excellent linearity (R2 = ≥0.999) and intraday retention time repeatability (≤ 0.31 %RSD) for ferulate and p-coumarate. The limits of detection and quantitation for these analytes were ≤ 39 nM and 130 nM, respectively. Furthermore, there was very little effect of the CWB sample matrix on the retention times of the analytes and analyte percent recoveries from the CWB sample matrix was ≥83.91%.

Testosterone-induced changes in phosphatidylcholine molecular species composition of Plasmodium chabaudi-infected erythrocytes

Parasitology ◽  
1993 ◽  
Vol 107 (5) ◽  
pp. 465-469
Author(s):  
S. Fiebig ◽  
A. P. Simões ◽  
F. Wunderlich ◽  
J. A. F. Op Den Kamp
Keyword(s):  
Species Composition ◽  
Blood Plasma ◽  
Molecular Species ◽  
Phospholipid Class ◽  
Erythrocyte Membranes ◽  
Plasmodium Chabaudi ◽  
Molecular Species Composition ◽  
Testosterone Treatment ◽  
Phospholipid Classes ◽  
Induced Changes

SUMMARYThis study is concerned with the influence of testosterone on the phospholipid class and the phosphatidylcholine molecular species composition of various fractions obtained from the blood of Plasmodium chabaudi-infected mice. Blood plasma, infected erythrocytes, isolated parasites and erythrocyte membranes isolated from both non-infected and infected erythrocytes in the form of ghosts were analysed. In general, the phospholipid classes remained unaffected, while the phosphatidylcholine (PC) molecular species composition showed differences after testosterone treatment. In infected erythrocytes, there was a decrease in 16:0/20:4-PC and 18:0/20:4-PC and an increase in 16:0/18:2(16:0/20:3)-PC. The decrease of 16:0/20:4-PC was exclusively confined to parasites. The rise in 16:0/18:2(16:0/20: 3)-PC and the diminution of 18:0/20:4-PC occurred in the erythrocyte membrane of both infected ghosts and non-infected ghosts as well as in the blood plasma. It is suggested that these changes occur primarily in the plasma thereby influencing the erythrocyte membranes. The decrease in 16:0/20:4-PC supports the view of the independence of the parasite from the biosynthetic lipid pathways of its host cell.

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