scholarly journals Computational mass spectrometry accelerates C=C position-resolved untargeted lipidomics using oxygen attachment dissociation

Author(s):  
Haruki Uchino ◽  
Hiroshi Tsugawa ◽  
Hidenori Takahashi ◽  
Makoto Arita

Abstract Mass spectrometry-based untargeted lipidomics has revealed the lipidome atlas of living organisms at the molecular species level. Despite the double bond (C=C) position being a crucial factor for enzyme preference, cellular membrane milieu, and biological activity, the C=C defined structures have not yet been characterized. Here, we present a novel approach for C=C position-resolved untargeted lipidomics using a combination of oxygen attachment dissociation and computational mass spectrometry to increase the rate of annotation. We validated the accuracy of our platform as per the authentic standards of 21 lipid subclasses and the biogenic standards of 51 molecules containing polyunsaturated fatty acids (PUFAs) from the cultured cells fed with various fatty acid-enriched media. By analyzing human and mice-derived biological samples, we characterized 675 unique lipid structures with the C=C position-resolved level encompassing 22 lipid subclasses defined by LIPID MAPS. Our platform also illuminated the unique profiles of tissue-specific lipids containing n-3 and/or n-6 very long-chain PUFAs (carbon M 28 and double bonds a 4) in the eye, testis, and brain of the mouse.

2003 ◽  
Vol 79 (3) ◽  
pp. 189-196 ◽  
Author(s):  
Fredoen Valianpour ◽  
Jacqueline J.M Selhorst ◽  
Lia E.M van Lint ◽  
Albert H van Gennip ◽  
Ronald J.A Wanders ◽  
...  

1984 ◽  
Vol 62 (3) ◽  
pp. 574-579 ◽  
Author(s):  
David A. Holden

Procedures are described for the synthesis of several azides, diimides, and azodiformates from long-chain alcohols and fatty acids. These reactive compounds have potential applications as thermal and photochemical curing agents, and as surface-modifying agents for the preparation of filled plastics and chromatographic packings. The surface activity of the compounds was characterized by investigations of their spreading behaviour in monolayers on water. Unlike the single-chain azides and azo compounds, which give well-defined monolayers at all temperatures, monolayers of diacyl diimides and dialkyl azodiformates with two long-chain substituents are unstable with respect to collapse to the bulk solid. The photoreaction of monolayers of octadecanoyl azide to give a mixture of products derived from an intermediate isocyanate was demonstrated by ir and mass spectrometry.


2018 ◽  
Vol 59 (8) ◽  
pp. 1510-1518 ◽  
Author(s):  
Sarah E. Hancock ◽  
Ramesh Ailuri ◽  
David L. Marshall ◽  
Simon H. J. Brown ◽  
Jennifer T. Saville ◽  
...  

2012 ◽  
Vol 302 (1) ◽  
pp. E1-E3 ◽  
Author(s):  
Matthew J. Watt ◽  
Andrew J. Hoy ◽  
Deborah M. Muoio ◽  
Rosalind A. Coleman

Plasma contains a variety of long-chain fatty acids (FAs), such that about 35% are saturated and 65% are unsaturated. There are countless examples that show how different FAs impart specific and unique effects, or even opposing actions, on cellular function. Despite these differing effects, palmitate (C16:0) is regularly used to represent “FAs” in cell based experiments. Although palmitate can be useful to induce and study stress effects in cultured cells, these effects in isolation are not physiologically relevant to dietary manipulations, obesity, or the consequences of physiological concentrations of FAs. Hence, authors should avoid conclusions that generalize about “FAs” or “saturated FAs” or “high-fat diet” effects if only a single FA was used in the reported experiments.


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