Multivariate Diversity Analysis of Quantitative Traits of Mung Bean [Vigna radiata (L.) Wilczek] Genotypes

Author(s):  
Tekle Yoseph ◽  
Firew Mekbib ◽  
Berhanu Amsalu ◽  
Zerihun Tadele

Abstract Mung bean is an important pulse crop grown by poor farmers in marginal and drought-prone areas of Ethiopia. Information on the extent of genetic divergence in mung bean is vital to identify diverse genotypes for crop improvement and the efficient utilization of the existing genetic resources. Therefore, the objectives of the study were to assess the extent and pattern of morphological diversity among the mung bean genotypes and to identify the traits contributing to the genetic diversity using multivariate analyses. The experiment was conducted using 60 mung bean genotypes at Jinka Agricultural Research Center during the 2018 cropping season. The first seven principal components explained 80.1% of the total variation. Almost all the studied traits were important contributors to the divergence. The cluster analysis based on quantitative traits revealed four distinct groups. The highest inter-cluster distance was recorded between cluster I and cluster IV (D 2  = 43.16 units). The minimum inter-cluster distance was noted between cluster III and cluster IV (D2 = 12.16 units). The maximum and minimum intra-cluster distances D2 were recorded within cluster I (D2 = 6.49 units) and cluster III (D2 = 3.53 units), respectively). The range of intra and inter-cluster distance was 3.53 to 6.49 units and 12.16 to 43.16 units, respectively. Hence, the high genetic distance exhibited within and among clusters has to be exploited through the crossing and selection of the most divergent parents for future mung bean breeding programs.

Author(s):  
Priyanka Jaiswal ◽  
. Banshidhar ◽  
. Banshidhar ◽  
Dan Singh Jakhar ◽  
Rajesh Singh

Thirty biofortified inbred lines of maize were evaluated for 11 parameters to study the genetic diversity by using D2 statistics during kharif 2017in Randomized Block Design (RBD) with three replications at Agricultural Research farm, Institute of Agricultural Sciences, BHU Varanasi. In present investigation all genotypes were grouped into ten cluster. Among the different clusters of inbred lines, the cluster II with 8 inbreds emerged as the largest cluster. The intra cluster D2 value ranged from 10.82 to 44.89. The maximum intra cluster distance was observed for cluster X (D2 = 44.89). The maximum inter cluster distance was observed between cluster V and VI (D2 = 180.90) followed by cluster V and VII (D2 = 166.10), cluster IV and V (D2 = 155.60), cluster V and VIII (D2 = 135.02) and cluster I and VI (D2 = 127.66). The maximum contribution towards divergence was due to 100 seed weight (52.18%), thus, estimates of variation in seed weight could be used as a basis for selection of distantly related parents. Highest mean value for grain yield per plant (80.8) and Zn concentration (39.53) were observed in cluster IV, while the highest mean value for 100 seed weight was found in cluster V. Therefore, these clusters prove to be of prime importance for selection of parents in hybridization programme aimed at higher yield along with enhanced grain Zn concentration.


Author(s):  
Rajendra Kumar ◽  
Ashwani Kumar ◽  
Ashwani Yadav ◽  
Renu Yadav ◽  
J. P. Misra ◽  
...  

Background: STMS markers and morphological traits were used to investigate the genetic relationship and allelic diversity in chickpea. In this study, we focused on the selection and more efficient utilization of core germplasm in breeding programs for chickpea crop improvement using STMS and quantitative / morphological traits. Methods: Seeds of elite accessions of chickpea were obtained from ICRISAT, Patancheru, Andhra Pradesh, India. 50 STMS markers and 11 quantitative traits were used for exploring the genetic variability and relationship in 35 chickpea accessions. Result: A total of 97 alleles were produced out of the 32 polymorphic STMS loci with an average of 3.03 alleles per locus ranging between 2-6 alleles per primer. The PIC value ranged from 0.029 to 0.768 with an average of 0.502. PIC value showed a highly positive correlation (r = 0.718) with number of alleles at the STMS loci. In both molecular and morphological markers / traits-based clustering, out of 35 chickpea accessions only one accession ICC-13892 was isolated at the end of clustering. The results indicated that highly polymorphic microsatellite markers NCPGR 68, NCPGR 50, NCPGR 81, NCPGR 48 and NCPGR 77 along with the accessions ICC-13892 having distant associations with ICC-13816, ICC-15697, ICC-15610, ICC-15868, ICC-15888, ICC-15996 with novel findings should be useful resources for strategies of allele mining, association genetics, mapping and cloning of gene(s) and in applied breeding to broaden the genetic base of chickpea.


2015 ◽  
Vol 50 (2) ◽  
pp. 160-167 ◽  
Author(s):  
Ricardo Meneses Sayd ◽  
Renato Fernando Amabile ◽  
Fabio Gelape Faleiro ◽  
Graciele Bellon

The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones were genetically more distant. For agronomic traits, two more consistent groupings were obtained, one with the most two-rowed materials, and the other with six-rowed materials. The more diverging materials were the two-rowed CI 13453, CN Cerrado 5, CN Cerrado 1, and CN Cerrado 2. The PI 356466, CN Cerrado 1, PI 370799, and CI 13453 genotypes show agronomic traits of interest and, as genetically different genotypes, they are indicated for crossing, in breeding programs.


2019 ◽  
Vol 12 (2) ◽  
pp. 39-60
Author(s):  
T. Margaritopoulou ◽  
D. Milioni

Abstract Sunflower, maize and potato are among the world’s principal crops. In order to improve various traits, these crops have been genetically engineered to a great extent. Even though molecular markers for simple traits such as, fertility, herbicide tolerance or specific pathogen resistance have been successfully used in marker-assisted breeding programs for years, agronomical important complex quantitative traits like yield, biotic and abiotic stress resistance and seed quality content are challenging and require whole genome approaches. Collections of genetic resources for these crops are conserved worldwide and represent valuable resources to study complex traits. Nowadays technological advances and the availability of genome sequence have made novel approaches on the whole genome level possible. Molecular breeding, including both transgenic approach and marker-assisted breeding have facilitated the production of large amounts of markers for high density maps and allowed genome-wide association studies and genomic selection in sunflower, maize and potato. Marker-assisted selection related to hybrid performance has shown that genomic selection is a successful approach to address complex quantitative traits and to facilitate speeding up breeding programs in these crops in the future.


Author(s):  
M A Madobe ◽  
M S Raihan ◽  
M Hasan ◽  
M S Biswas

Characterization of genetic diversity is the foundation step for crop improvement, which provides a basis for analyzing combining ability and heterosis of inbred genotypes during a hybridization program. An investigation was carried out at the field laboratory of the Genetics and Plant Breeding department in Bangabandhu Sheikh Mujibur Rahman Agricultural University, to elucidate the genetic architecture by evaluating 12 morphological and 4 molecular (SSR) markers within 52 diverse S1 genotypes, and to assess the relationship of molecular and morphological GD. An almost equal amount of PCV and GCV coupled with high heritability and genetic advance for the traits cob weight (gm), NKPC, and NKPR lead to the selection of promising genotypes based on these characters. Correlation coefficient and scatter plot matrix established a positive and strong relationship of KL (mm), KW (mm), and KT (mm) with 100 kernel weight (gm) suggesting the importance of kernel morphology. Mahalanobis D2 statistics revealed the highest inter-cluster distance between I and II. The percentages of molecular variance within the population and among the population were 76% and 14 %, respectively. The optimum K-value was 5. Heatmap relying on molecular GD exposed MMIL-28, MMIL-54, and MMIL-96 as the most diverse lines. SHE analysis hypothesized the increase of richness and diversity over time. Less correlation between the divergence generated from morphological traits and molecular markers suggested that the morphological variation may be determined by environmental factors and also by genetic factors. A strategy for the effective selection of predicting parental lines for a future hybridization program was developed.


2021 ◽  
pp. 27-32
Author(s):  
G. Ya. Krivosheev ◽  
A. S. Ignatiev

The selection of pairs for hybridization requires knowledge about the correlation of the most important quantitative traits that affect grain yield of maize hybrids. The current study was carried out in the Agricultural Research Center “Donskoy” located in the south of the Rostov region with unstable moisture. The years of study (2018–2020) were arid (HThC 0.32–0.89). The purpose of the current study was to evaluate the correlation between quantitative traits and their influence on grain yield of maize hybrids under arid conditions. The objects of research were 96 interline maize hybrids. The analysis has identified the correlation between grain yield and such quantitative traits as ‘one maize ear weight’ (r = 0.64...0.87), ‘number of grains per one maize ear row’ (r = 0.37...0.75), ‘number of grains per maize ear’ (r = 0.32...0.51), ‘number of maize ears per plant’ (r = 0.41...0.53), ‘grain yield’ (r = 0.45...0.64). The traits ‘1000-grain weight’ and ‘number of grain rows’ had either no or slight effect on the formation of grain yield of maize hybrids (r = -0.12...0.28). There have been established the traits, the high values of which were well-combining in one genotype. The values of the trait ‘one maize ear weight’ raised due to an increase of the trait ‘number of grains per one maize ear row’ (r = 0.27...0.74), ‘number of grains per maize ear’ (r = 0.26...0.55), ‘grain yield’ (r = 0.21...0.52). The trait ‘number of grains per maize ear’ raised with an increase in the values of such constituent components as ‘number of grains per one maize ear row’ (r = 0.70...0.76), ‘number of grain rows per maize ear’ (r = 0.59...0.66), and also with an increase of ‘number of maize ears per plant’ (r = 0.32...0.51) and ‘grain yield’ (r = 0.36...0.38). There have been identified difficulty-combining quantitative traits, when the value of the trait ‘1000-grain weight’ decreased with the increase of the ‘number of grain rows per maize ear’ (r = – 0.18...-0.56), ‘number of grains per a maize ear row’ (r = -0.15...-0.31) and ‘grain yield’ (r = -0.01...-0.36).


Author(s):  
Yolanda del Rocio Moreno-Ramírez, Amalio Santacruz-Varela ◽  
Pedro A. López, Higinio López-Sánchez ◽  
L. Córdova-Téllez, V. A. González-Hernández, T. Corona-Torres ◽  
Ricardo López- Ortega

Guajillo (Capsicum annuum L.) is one of the five more important chile types used as food in Mexico and the most important to local economy in Zacatecas, whose production is supplied mainly by native populations. In spite of this, not much is known about the morphological diversity of Guajillo landraces of Zacatecas. Here, we describe and quantify their morphological variation in order to establish breeding programs and to preserve the phenotypic diversity. Thirty-two plant, flower, fruit, seed and phenological traits were assessed in 96 Guajillo chile landraces and four improved varities of Guajillo used as controls, grown during the 2015 agricultural cycle in Zacatecas, Mexico. The selection of variables was made based on the analysis of variance and multivariate analysis that were applied to the selected variables to estimate the morphological diversity. A wide variation of plant, fruit and seed characteristics was identified. This morphological heterogeneity is attributed mainly to fruit traits such as: size, weight, shape, color, transverse wrinkling of the fruit, shape of the peduncle and the number of fruits produced. This wide morphological diversity can potentially be utilized in the genetic improvement of Guajillo chile.


2020 ◽  
Vol 38 (2) ◽  
pp. 217-223
Author(s):  
Giovani O da Silva ◽  
Caroline M Castro ◽  
Fernanda Q Azevedo ◽  
Arione S Pereira ◽  
Agnaldo DF de Carvalho ◽  
...  

ABSTRACT The objective of this study was to evaluate the yield potential, frying quality, plant vigor, and vegetative cycle of Chilean potato clones, aiming to identify those with potential to become new cultivars or to be used in breeding programs. The experiments were conducted in Pelotas-RS and Canoinhas-SC, Brazil, in spring 2017. A set of 37 advanced potato clones belonging to the Agricultural Research Institute of Chile (INIA) was compared to commercial cultivars Agata, Asterix and Atlantic. A randomized complete block design with three replications of two rows with 10 plants each was used. Tuber yield, frying quality, plant vigor and vegetative cycle traits were evaluated. The data were submitted to analysis of variance, test for grouping means, and the selection differential. In this set of clones there are some presenting superior quality in relation to marketable tuber yield and frying quality, but the vegetative cycle was not shorter nor plant vigor was lower. Clones CH 27 and CH 44 were the best for marketable tuber yield and frying quality at both locations.


2018 ◽  
Vol 6 ◽  
pp. 52-57
Author(s):  
Hom Nath Pathak

Agro-morphological traits are the features of cultivated rice i.e. Oryza sativa L. Rice landraces differ from the other landrace in morphological features such as grain weight, culm number, and height of the plant. Nineteen different landraces of cultivated rice from Paiyunpata village, Baglung were studied and their quantitative characters were measured. The most varied landraces in morphological characters (Khate, Rato, Nepale, Anadi etc. i.e. species far from quadrants in PCA) are suggested for crop improvement. Findings from this research could be helpful for germplasm conservation.


2021 ◽  
Author(s):  
VISHNU V S ◽  
P.M. RADHAMANY

Abstract The crops that are not commercially grown or widely traded are known as underutilized or unusable crops. Most of the legumes have come under these categories. Giving importance to such neglected and underutilized crops is an effective way to maintain a diverse and healthy diet and to combat micronutrient deficiencies. Lablab purpureus (L.) Sweet is one of the most important underutilized legume vegetable crops, which exhibits broad genetic variability in multiple characteristics such as the habit, pigmentation of the stem, and color of the pod. In the past, researchers paid very little attention to the systematic crop improvement of L.purpureus. In the present study, fifty accessions of L. purpureus were collected from different places of Kerala and Tamil Nadu and analyzed for yield, yield contributing characters, and biochemical factors. Nutritional and anti-nutritional factors were analyzed using the standard protocol. The accessions exhibited significant variations for all the characters tested. The nutritional content of all the accessions were found to be high, whereas anti-nutritional factors were very low. The differences in phenotypic and genotypic coefficient of variation was compared for different traits, and it was found to be very narrow for almost all the characters studied. The fifty genotypes involved in the study varied in the number of pod/ inflorescence, length and width of the pod, number of flower/ inflorescence, and length of the peduncle. So these observations can be used as a baseline data for the selection of parental lines for future improvement of Lablab purpureus.


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