scholarly journals Integrative Analysis of Transcriptomics and Metabolomics to Reveal the Melanogenesis Pathway of Muscle and Related Meat Characters in Wuliangshan Black-Boned Chickens

Author(s):  
Tengfei Dou ◽  
Shixiong Yan ◽  
Lixian Liu ◽  
Kun Wang ◽  
Zonghui Jian ◽  
...  

Abstract Background: Melanin is an important antioxidant in food, and has been used in medicine and cosmetology. Chicken meat with high melanin content from black-boned chickens have been considered a high nutritious food with potential medicinal properties. The molecular mechanism of melanogenesis of skeletal muscle in black-boned chickens remain poorly understood. This study investigated the biological gene-metabolite associations regulating the muscle melanogenesis pathways in Wuliangshan black-boned chickens with two normal boned chicken breeds as control.Results: We identified 25 differentially expressed genes and 11 transcription factors in the melanogenesis pathways. High levels of the meat flavor compounds inosine monophosphate, hypoxanthine, lysophospholipid, hydroxyoctadecadienoic acid, and nicotinamide mononucleotide were found in Wuliangshan black-boned chickens.Conclusion: Integrative analysis of transcriptomics and metabolomics revealed the dual physiological functions of the PDZK1 gene, involved in pigmentation and/or melanogenesis and regulating the phospholipid signaling processes in muscle of black boned chickens.

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Jing Sun ◽  
Yan Wang ◽  
Nianzhen Li ◽  
Hang Zhong ◽  
Hengyong Xu ◽  
...  

The objective of the study is to compare the effects of free-range (FR) and cage-range (CR) breeding on gut microbiota and flavor compounds of Caoke (C) and Partridge Shank chickens (Q). A total of 120 experimental chickens were assigned to FR group and CR group; each group contain both 30 Caoke chickens and 30 Partridge Shank chickens. At 154 d old, 12 chickens of each group were selected and their cecal contents were extracted and examined for the composition of gut microbiota by illumina sequencing of the V3 region of the 16S rDNA genes, and flavor compounds were analyzed through headspace-solid-phase microextraction (HS-SPME) method. The results showed that, except for acids, the amount of flavor substances in the FR group was higher than those in the CR group, especially the content of Hexanal and D-limonene. Meanwhile, the higher concentrations of carbonyls including (E,E)-2,4-decadienal, (E)-2-decenal, (E)-2-octenal, and pentanal were in the FR chicken meat, but the differences in concentrations compared with CR were not significant. High levels of ethyl hexanoate and β-ocimene were only detected in FR groups. The Firmicutes had the highest proportion of chicken cecal microbiota, whereas the Fusobacteria was only detected in the cecal samples of Q chicken in FR group. Actinobacteria was more prevalent in FR groups than in CR groups. Meanwhile, in Q chickens, the proportions of Bacteroidetes and Proteobacteria in FR group were higher than those in CR group. Using MG-RAST Subsystem Technology, we found that some genes were associated with the formation of precursors of flavor compounds or with the metabolism and degradation of aromatic compounds. Overall, CR and FR breeding influenced the gut microbiota and flavor compounds, potentially because of the changes in diet and living conditions.


2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Timothy V. Murphy ◽  
Lindsay Wu ◽  
Shaun L. Sandow ◽  
Abhirup Das ◽  
Hussein Naim

2021 ◽  
pp. 110823
Author(s):  
Ao Sun ◽  
Wei Wu ◽  
Olugbenga P. Soladoye ◽  
Rotimi E. Aluko ◽  
Kathrine H. Bak ◽  
...  

2019 ◽  
Author(s):  
Marshall Lukacs ◽  
Jonathan Gilley ◽  
Yi Zhu ◽  
Giuseppe Orsomando ◽  
Carlo Angeletti ◽  
...  

AbstractThe three nicotinamide mononucleotide adenylyltransferase (NMNAT) family members synthesize the electron carrier nicotinamide adenine dinucleotide (NAD+) and are essential for cellular metabolism. In mammalian axons, NMNAT activity appears to be required for axon survival and is predominantly provided by NMNAT2. NMNAT2 has recently been shown to also function as a chaperone to aid in the refolding of misfolded proteins. Nmnat2 deficiency in mice, or in its ortholog dNmnat in Drosophila, results in axon outgrowth and survival defects. Peripheral nerve axons in NMNAT2-deficient mice fail to extend and innervate targets, and skeletal muscle is severely underdeveloped. In addition, removing NMNAT2 from established axons initiates axon death by Wallerian degeneration. We report here on two stillborn siblings with fetal akinesia deformation sequence (FADS), severely reduced skeletal muscle mass and hydrops fetalis. Clinical exome sequencing identified compound heterozygous NMNAT2 variant alleles in both cases. Both protein variants are incapable of supporting axon survival in mouse primary neuron cultures when overexpressed. In vitro assays demonstrate altered protein stability and/or defects in NAD+ synthesis and chaperone functions. Thus, both patient NMNAT2 alleles are null or severely hypo-morphic. These data indicate a previously unknown role for NMNAT2 in human neurological development and provide the first direct molecular evidence to support the involvement of Wallerian degeneration in a human axonal disorder.


Epigenetics ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. 1557-1569 ◽  
Author(s):  
Maléne E Lindholm ◽  
Francesco Marabita ◽  
David Gomez-Cabrero ◽  
Helene Rundqvist ◽  
Tomas J Ekström ◽  
...  

2015 ◽  
Vol 94 (9) ◽  
pp. 2266-2271 ◽  
Author(s):  
L.L. Cui ◽  
J.F. Wang ◽  
K.Z. Xie ◽  
A.H. Li ◽  
T.Y. Geng ◽  
...  
Keyword(s):  

2017 ◽  
Vol 6 (8) ◽  
pp. 819-832 ◽  
Author(s):  
Rachel S. Fletcher ◽  
Joanna Ratajczak ◽  
Craig L. Doig ◽  
Lucy A. Oakey ◽  
Rebecca Callingham ◽  
...  

1993 ◽  
Vol 76 (7) ◽  
pp. 2528-2536 ◽  
Author(s):  
Ulrich A. Huber ◽  
Desir�e Bergamin
Keyword(s):  

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