scholarly journals Establishment of a Highly Sensitive Analytical Method For Measurement of Monoamine Neurotransmitters Using Mass Spectrometry

Author(s):  
Anri Hirai ◽  
Ryo Yamazaki ◽  
Atsushi Kobayashi ◽  
Takashi Kimura ◽  
Kei Nomiyama ◽  
...  

Abstract Monoamine neurotransmitters (MAs), including dopamine (DA) and serotonin (5-HT), regulate brain functions such as behavior, memory, and learning. Neonicotinoids are pesticides that are being used more frequently. Neonicotinoid exposure has been observed to produce neurological symptoms, such as altered spontaneous movements and anxiety-like behaviors, which are suspected to be caused by altered MA levels. However, current neurotoxicity tests are not sufficiently sensitive to make these determinations. In this study, we performed some behavior tests and developed a sensitive and accurate analytical method using liquid chromatography-mass spectrometry (LC-MS/MS) to clarify the effects of neonicotinoid administration on MAs in the brain.We orally administered the neonicotinoid imidacloprid (0, 10, and 50 mg/kg body weight) to C57BL/6NCrSlc mice. In behavior tests, the decrease of activity was observed. The LC-MS/MS quantification of MAs in various brain regions by tetrafluoroborate salt of 2,4-diphenyl-pyranylium (DPP) derivatization, which was newly developed in this study, showed a decrease in some MA levels in the olfactory bulb and the striatum.Thus, in this study, we developed a new method for the sensitive detection of MAs by LC/MS using DPP derivatization. In addition, we showed that this sensitive MA quantification is effective in clarifying the neurotoxicity caused by chemical substances.

2017 ◽  
Vol 9 (39) ◽  
pp. 5749-5756
Author(s):  
R. Nageswara Rao ◽  
K. Guru Prasad ◽  
Sara Khalid ◽  
K. S. Praneetha ◽  
Dilip Dodda ◽  
...  

A simple, rapid and highly sensitive liquid chromatography-mass spectrometry method was developed and validated for analysis of iloperidone on rat dried blood spots.


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