scholarly journals Changes in The Liver Transcriptome and Physiological Parameters of Japanese Black Steers During The Fattening Period

Author(s):  
Minji Kim ◽  
Tatsunori Masaki ◽  
Kentaro Ikuta ◽  
Eiji Iwamoto ◽  
Yoshinobu Uemoto ◽  
...  

Abstract We investigated the physiological changes during the fattening period and production characteristics in Japanese Black steers bred and raised using the typical feeding system in Japan. Here, 21 Japanese black steers aged 12 months were used, and the experimental period was divided into early (12–14 months of age), middle (15–22 months), and late fattening phases (23–30 months). The liver transcriptome, blood metabolites, hormones, and rumen fermentation characteristics were analyzed. The blood triglyceride and non-esterified fatty acid concentrations increased, and blood ketone levels decreased, with fattening phases. Blood insulin increased with fattening phases and was higher in groups with high carcass weight and marbling. Rumen fermentation characteristics showed high propionate levels and low butyrate levels in late fattening phases, likely due to increased energy intake. Genes related to glucose metabolism, such as SESN3, INSR, LEPR, and FOXO3, were down-regulated in late fattening phases. Genes related to lipid metabolism, such as FABP4, were up-regulated, whereas FADS1 and FADS2 were down-regulated. These findings suggest that the physiological changes resulted from changes in the energy content and composition of diets. Liver metabolism changed with changes in fat metabolism. Insulin was strongly associated with physiological changes and productivity in Japanese Black cattle.

2008 ◽  
Vol 62 (3-4) ◽  
pp. 133-142 ◽  
Author(s):  
Mirjana Joksimovic-Todorovic ◽  
Slavca Hristov ◽  
Vesna Davidovic ◽  
Renata Relic ◽  
Branislav Stankovic

Behavior of cattle is a simple and easily established indicator of their health condition, production characteristics and welfare, showing whether and how the animal has adapted to the maintenance conditions. Essentially, all forms of cattle behavior are accompanied by certain physiological changes in the organism, and the basic moving forces of behavior are congenital. The moving forces of behavior of cattle are narrowed down to a certain number of biological needs (the need for food, water, sexual and other biological needs) and congenital urges and instincts, such as the combative and maternal instincts. Cattle are grazing animals and they cannot exhibit all their congenital natural activities of behavior under intensive maintenance conditions. Different internal and external stimuli influence the types of behavior of cattle, changing the motivational activities of their organism. In the course of domestication, certain forms of behavior of cattle have sustained changes, some have adapted to the new conditions, and new ones have appeared as well. The social, reproductive, maternal, and feeding behavior of cattle in closed maintenance conditions has not changed fundamentally, but the model of its manifesting has changed. Furthermore, certain disorders in the behavior of cattle also appear as a consequence of the maintenance conditions, and they can also be of hereditary character. In order to promote welfare, cattle should be enabled to exhibit their natural behavior, but they should also be provided with an environment that has natural characteristics.


1976 ◽  
Vol 23 (2) ◽  
pp. 197-209 ◽  
Author(s):  
J. C. Tayler ◽  
K. Aston

SUMMARY1. Young adult and adult British Friesian female cattle (heifers and cows) which were lactating were individually fed on diets of grass silage ad libitum with supplements of dried grass, dried grass/barley or barley/groundnut in two randomized block experiments.2. In Experiment I, equal quantities of dry matter (DM) as dried grass in the form of a wafer (W), cob (C) or pellet (P) were given with the silage during weeks 8 to 18 of lactation, to three groups of 12 heifers. Silage DM intakes were respectively 14·2, 14·3 and 15·8 ± 0·43 g/kg live weight per day (P<0·05). Milk yields were 14·0, 15·6, 16·7 ± 0·35 kg/day (P< 0·001). Milk composition and live-weight change did not differ significantly.3. In Experiment II, five treatment groups of heifers and cows were offered silage with equal amounts of a supplement of either dried grass pellets (treatment 1) or dried grass/barley in the ratios of 2: 1 (treatment 2), 1: 2 (treatment 3), or a barley/groundnut supplement (treatment 4). In treatment 5 the cattle received the same supplement as in treatment 4, but at a 10% higher level. Silage DM intakes for treatments 1 to 5 during lactation weeks 6 to 22 were 8·64, 8·94, 7·96, 6·96, 7·63 kg/day (1, 2>4, P<0·01; 2>5, P<0·05). The intake of digestible organic matter, milk yields and live-weight changes over the whole experimental period were not influenced significantly by treatments 1 to 4, but in treatment 5 the milk was significantly higher in fat, protein and energy content than in treatments 1 to 4. Blood composition was normal and did not differ markedly between treatments.


2001 ◽  
Vol 204 (12) ◽  
pp. 2175-2183 ◽  
Author(s):  
G. Parra ◽  
M. Yúfera

SUMMARY Growth, energy content, ingestion and respiration rates and energetic efficiencies were measured in the larvae of two marine fish (Solea senegalensis and Sparus aurata) whose eggs have a similar diameter (approximately 1mm) and energy content (approximately 1J), but whose larvae reach a quite different mass after the first month of life. Experiments were carried out in populations reared under the same conditions in the laboratory during the first month after hatching. Solea senegalensis grow from hatching to the start of metamorphosis (approximately day 14) at twice the rate of Sparus aurata (specific growth rate for Solea senegalensis 0.25μgday−1; specific growth rate for Sparus aurata 0.12μgday−1). The tissues in Solea senegalensis larvae accumulated energetic reserves that were used during metamorphosis, which occurred during the third week after hatching. Ingestion and respiration rates differed in the two species during the experimental period. Although Solea senegalensis continued eating during metamorphosis, the specific ingestion rates decreased during the process. Nevertheless, no great differences in specific ingestion rates and rates of oxygen consumption were observed when comparing the same larval mass range. Larvae of both species showed an allometric relationship between respiration rate and biomass. The energetic efficiencies calculated in the present study denoted different metabolic patterns in each species. In Solea senegalensis, the energy used for growth increased progressively during the larval (pelagic) period and then, from the first signs of metamorphic transformation, remained almost constant. In this species, the energy allocated to growth was greater than that allocated to metabolic processes. Sparus aurata invested less energy in growth than in metabolic processes and displayed a constant energy allocation throughout the experimental period. During the first month after hatching, Solea senegalensis always allocated more energy for growth than did Sparus aurata.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 71-71
Author(s):  
Nicole T Briggs ◽  
Bayissa Hatew ◽  
Michael A Steele

Abstract Inorganic sources of trace minerals are commonly supplemented in dairy cow diets; however, there has been an increase in the supplementation of minerals complexed with organic compounds. These organic trace minerals are thought to have greater bioavailability which may enhance rumen fermentation and absorption. The objective of this study was to assess the effects of dietary concentration and source of supplemental trace minerals on serum trace mineral status and rumen fermentation. Six lactating Holstein cows were used in a 6 x 6 Latin square design with a 23-day adaptation and 5-day experimental period. Cows were fed the same basal diet daily except for the difference in source [organic (ORG) versus inorganic (INO)] and concentration (50%, 100%, and 200% based on NRC recommendations) of trace mineral supplemented. During the experimental period feed intake and blood were collected daily. Rumen fluid was collected on the final two days of the experimental period. Data was analyzed with PROC MIXED in SAS 9.4. Dry matter intake (18.1 ± 0.70 kg), serum mineral concentrations (Cu, Mn, Se, and Zn), and rumen pH (6.5 ± 0.64) did not differ among the treatments. However, serum concentration of Co was higher in 200% ORG compared to 50% and 100% INO and 50% ORG. Ruminal concentration of acetate was higher in 50% and 100% ORG compared to 200% ORG. Butyrate ruminal concentration was higher at 50% ORG compared to 200% ORG. Ruminal propionate concentration was higher in 50% INO and 50% ORG compared to 100% INO and 200% ORG. These findings demonstrate serum trace mineral status and ruminal pH are not tightly controlled by the source of trace minerals when supplemented at 50%, 100% and 200% of the NRC recommendations, however rumen fermentation may be affected by the dietary concentration of trace minerals in the diet.


Author(s):  
Preeti . ◽  
Neelam Kewalramani ◽  
S. S. Kundu ◽  
Amit Sharma

Twenty growing male Murrah calves, with average body weight (kg) 220±5, were randomly assigned to five treatments of water containing total dissolved solids (TDS; ppm) viz CON=500; T1=2,000; T2=4,000; T3=6,000 and T4=8,000 at National Dairy Research Institute (NDRI), Karnal India. Animals were arranged in a randomized block design with 120-day experimental period. Feed and water intake were measured daily, rumen fermentation parameters and blood profile were evaluated at monthly interval. Water intake was apparently higher in T1 (17.46 l/day) and T2 (16.77 l/day) as compared to CON (control; 14.07 l/day) but did not differ significantly. However, water intake was lower (P less than 0.05) in T3 (10.40 l/day) and T4 (8.18 l/day) as compared to group T1, T2 and T3CON. Except ammonia nitrogen which was decreased with increasing TDS of water, all other rumen liquor parameters were found similar. In blood parameters, there was significant decrease (P less than 0.05) in plasma glucose, total protein, and albumin while there was significant increase (P less than 0.05), in AST and ALT with increasing TDS of water. Blood Urea Nitrogen, creatinine, uric acid were not affected. On the basis of above results it was inferred that water intake was decreased at higher TDS (above 4000 ppm)


1993 ◽  
Vol 44 (3) ◽  
pp. 363 ◽  
Author(s):  
RA Leng

Knowledge of quantitative digestion and metabolism in ruminants was developed most rapidly when isotope dilution techniques became easy to apply, facilitated by improved instrumentation and mathematical approaches. The Armidale group led by Professor E. F. Annison and Dr D. B. Lindsay were at the forefront of these developments in the late 1950's. Since then knowledge in this area has developed at an ever increasing rate. The data that accumulated from the quantitative approach led to simple or complex models of animal digestion, metabolism and growth. These in turn led to much questioning of the dogma of feed evaluation and feeding standards as they applied in practice, especially for ruminants fed on poor quality forages. The knowledge that developed has clearly shown that the way toward substantial increases in productivity of ruminants on forage based diets is through the balanced nutrient approach that considers the efficiency of the rumen ecosystem and the availability of dietary nutrients post-ruminally. With increasing emphasis on quality-beef markets at the present time, it seems likely that the time is ripe for application of much of this knowledge. The major breakthroughs have come about by recognition of the nutrients required to balance a ruminant's diet where the animal depends on the end-products of rumen fermentation (i.e. on a forage-based diet). When this is achieved, the increase in efficiency of use of nutrients lifts the overall nutrition of the animal to a level that is well above that predicted from feeding standards, based on the metabolizable energy content of the supplement or the total diet. This understanding, together with the stoichiometry of rumen fermentation, has indicated an important approach to help ameliorate the greenhouse effect, that is, lowering of enteric methane production per unit of feed intake or per unit of animal products from ruminants by strategic supplementation.


1993 ◽  
Vol 56 (1) ◽  
pp. 85-92 ◽  
Author(s):  
D. Cardenas Garcia ◽  
C. J. Newbold ◽  
H. Galbraith ◽  
J. H. Topps ◽  
X. B. Chen ◽  
...  

AbstractThe effect of including 14 g urea with either 75 g dry matter (DM) from sugar cane molasses (UM) or Colombian rice polishings (RP) at three levels, 68 (RP1), 137 (RP2) or 203 (RP3) g DM on grass hay DM intake and on rumen fermentation was investigated. An incomplete Latin-square design was used and each experimental period was divided into 12 days for adaptation to each diet followed by 9 days in metabolism cages when all measurements were made using five adult sheep.Pooled mean values for rumen metabolites (five samples per day) were calculated. Rumen pH was not affected by the nature of the supplements. Concentrations of volatile fatty acids (VFA) (UM 92·8, RP1 84·2, RP2 86·4, and RP3 84·0 (s.e.d. 3·4) mmol/l) and lactate (UM 2·0, RP1 1/6, RP2 1/7, RP3 1/8 (s.e.d. 0·014) mmol/l) (UM v. RP, P < 0·05 and P < 0·10 respectively) were lower when RP were given, while concentration of branched and longer chain VFA (26·5, 34·0, 31·1 and 33·5 (s.e.d. 1·6) mmol/mol total VFA, UM v. RP, P < 0·01) and ammonia (98, 131, 141, 137 (s.e.d. 16·1) mg/l, UM v. RP, P < 0·05) were increased. Numbers of rumen protozoa (1·6, 3·2, 2·7, 3·3 (s.e.d. 0·75) × 105 per ml, UM v. RP, P < 0·20) tended to be higher 2 h after feeding when RP rather than UM were given. However, hay DM intake (1050, 960, 960, 880 (s.e.d. 45·3) g/day, UM v. RP, P < 0·05), and microbial protein supply (11·7, 9·3,11·1,10·8 (s.e.d. 0·59) g N per day, UM v. RP, P < 0·05), estimated from urinary purine excretion were reduced by feeding RP instead of UM.At the levels of inclusion tested RP did not increase the efficiency of rumen fermentation and were not as effective a supplement with urea as was molasses for a low-quality forage diet.


2021 ◽  
Author(s):  
Barbara Stefańska ◽  
Frank Katzer ◽  
Barbara Golińska ◽  
Sebastian Smulski ◽  
Patrycja Sobolewska ◽  
...  

Abstract Background The aim of this study was to evaluate whether different methods of providing eubiotic feed additives, to neonatal calves during the preweaning period, can improve the health, performance, rumen fermentation, and metabolic status of the calves. Forty-four Holstein-Friesian dairy calves, were divided into one of four treatment groups for the 8 week trial. The eubiotic feed additives consisted of a combination of probiotic Lactobacillus spp. (multiple-strains) at a dose of 250 mg/calf/d and phytobiotics containing rosmarinic acid, as the main bioactive compound, at a dose of 50 mg/calf/d. Treatment differed by the methods that the eubiotic feed additives were provided, and the groups were as follows: CON (control, without eubiotic feed additive in their milk replacer or their starter feed), MR (eubiotic feed additives added to the milk replacer), SF (eubiotic feed additives added to the starter feed), MRS (eubiotic feed additives added to the milk replacer and the starter feed). Individual intake of starter feed was measured daily and body weights weekly until 56 d of age. Results The body weight of the MR treatment group calves were higher on days 28 and 56. Including the eubiotic feed additive in the milk replacer affected increasing average daily gain, starter intake and total dry matter intake from d 29 to d 56 and the overall experimental period in comparison to the CON group. The calves of the MR treatment had lower fecal scores from d 3 to d 28, number of parasite oocysts/cysts per gram of feces on d 28, and the occurrences of faecal consistency scores of 3 (mild diarrhea) and 4 (confirmation diarrhea) were 3.2 and 3.0 times lower, respectively, compared with animals of the CON group. Also, in this group higher ruminal concentrations of total volatile fatty acids, propionate, and butyrate were noted on d 56 compared to CON group. Adding eubiotics into milk replacer resulted the highest concentrations of blood insulin-like growth factor-I and β-hydroxybutyrate from d 29 to 56 and through the entire experimental period. Conclusion The addition of eubiotic feed additives into the milk replacer can improve health, performance, rumen fermentation, and biochemical blood indices in dairy calves during the preweaning period.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 173-174
Author(s):  
Tianyu Chen ◽  
Jianxin Xiao ◽  
Tingting Li ◽  
Jing Ma ◽  
Zhijun Cao

Abstract To determine the appropriate time to feed hay to calves, 210 healthy Holstein calves at d 2 were randomly divided into 3 groups: feeding basic diet (starter) without hay (CON), inclusion of oat hay from the second week (H2) or fourth week (H4) in addition to the basic diet. All calves were weaned on d 56 and raised in the same hutches until the end of the experiment (d 70). Calf starter intake and fecal score were recorded daily throughout the experimental period. Body weight, body size, blood and rumen fluid samples were collected every 2 weeks before weaning and once a week after weaning. Inclusion of oat hay in the basic diet (H2 and H4) resulted in increased body weight (P &lt; 0.01) and average daily gain (P &lt; 0.01) throughout the experiment when compared to the basic diet (CON) only. Calves fed oat hay from 2 weeks of life had the highest starter intake (P &lt; 0.01), average daily gain (P &lt; 0.01) in the pre- and post-weaning periods. Body length and heart girth was similar between treatments. The H2 calves had lower diarrhea frequency (P &lt; 0.05) and diarrhea days (P &lt; 0.05) during the post-weaning period. Compared to the CON calves, inclusion of hay increased the rumen pH (P &lt; 0.01), while decreasing the NH3-N (P &lt; 0.01) and tend to decrease the total VFA (P &lt; 0.06) during the entire trial. Calves in H2 treatment spent more time ruminating (P &lt; 0.01), but less time standing (P &lt; 0.05), eating starter (P &lt; 0.01) and engaging in abnormal behaviors (P &lt; 0.01). In conclusion, calves supplemented with oat hay improved growth rate and rumen fermentation when compared with calves fed only starter, and adding hay from 2 weeks of calves life may be the best choice for the farm to have the greatest growth and health performance.


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