scholarly journals Rock2 Regulates Proliferation and Differentiation of Chondrocytes in Osteoarthritis through β-catenin Signaling

Author(s):  
Liang Hao ◽  
Jun Chen ◽  
Xiliang Shang ◽  
Wu Yang ◽  
Shiyi Chen

Abstract BackgroundOsteoarthritis (OA) adversely affects quality of life of elderly patients and is among hotspots and challenges of current research efforts. However, mechanism of occurrence and development of OA has not been fully elucidated. MethodsThrough qRT-PCR and Western blot assays, the current study established that levels of Rock2, a key protein in Rho signaling pathway, were significantly higher in OA cartilage. Furthermore, the current study explored effect of down-regulating Rock2 expression on growth and apoptosis of cartilage cells using CCK-8, Edu and Flow cytometry assays. Alkaline phosphatase (ALP) and Alizarin Red S (ARS) assays were then used to determine differentiation effects.ResultsFindings showed that expression of Rock2 is closely related to proliferation, apoptosis and differentiation of chondrocytes. Furthermore, the current study confirmed that Rock2 affects growth and differentiation of chondrocytes by activating β-catenin signaling pathway. Conclusionthe current study provided novel insights to targeted therapy of OA.

2020 ◽  
Vol 28 (1) ◽  
pp. 252-259
Author(s):  
Warren Fingrut ◽  
Wendy Davis ◽  
Eric McGinnis ◽  
Karen Dallas ◽  
Khaled Ramadan ◽  
...  

Salvage options for patients with relapsed B-cell acute lymphoblastic leukemia (B-ALL) include inotuzumab ozogamicin (InO), a recombinant, humanized anti-CD22 monoclonal antibody conjugated to the cytotoxic antibiotic calicheamicin. However, the benefit of InO in patients with dim CD22 expression remains unclear. We present a case of a patient with B-ALL who responded to InO despite only dim surface expression of CD22 by flow cytometry, achieving a survival benefit concordant with that reported in the literature and maintaining a good quality of life as a transfusion-independent outpatient. Our observation has broad relevance to clinicians who manage patients with B-ALL who are candidates for InO.


2022 ◽  
Author(s):  
Ruofei Du ◽  
Xin Wang ◽  
Huiyue Zhou ◽  
Lixia Ma ◽  
Leon M. Larcher ◽  
...  

Abstract Purpose This study was to assess the status of quality of life and explore the possible factors correlated with quality of life among non-small cell lung cancer (NSCLC) patients with skin adverse drug reactions under targeted therapy. Methods We performed a cross-sectional study including 536 NSCLC patients with skin adverse drug reactions by targeted therapy in cancer outpatient clinics of three hospitals in China between May 2020 and May 2021. And we collected data with structured questionnaires and identified the relationships among coping style, self-management and quality of life by Pearson correlation analysis and multiple linear regression algorithm. Results The total score of quality of life was 46±12.84 in 536 NSCLC patients with skin adverse drug reactions undergoing targeted therapy. In multiple linear regression analysis, we identified the significant factors associated with quality of life including age, education level, combination of medicine, Charlson Comorbidity Index (CCI), stages of disease, facing, yield, symptom management, daily activity management, psychological and emotional management, self-efficacy and self-management (P < 0.05). Conclusions NSCLC patients with skin adverse drug reactions undergoing targeted therapy generally had a compromised quality of life. And the critical factors that affected the status of quality of life were age, education level, co-morbidity, the combinatorial application of drugs and stage of disease, self-management and coping styles.


2019 ◽  
pp. 142-145
Author(s):  
L. M. Kogonia ◽  
E. V. Markarova ◽  
G. A. Stashuk ◽  
M. M. Byakhova ◽  
M. M. Akhmetov

The article describes the experience in effective use of the second-generation EGFR II blocker in palliative target therapy of EGFRnegative lung adenocarcinoma. The use of afatinib in the fifth-line palliative therapy of EGFR-negative lung adenocarcinoma allowed us to achieve a significant positive response to therapy in the lungs and improve the patient’s quality of life.


2021 ◽  
Author(s):  
Yuxi Liu ◽  
Jinghao Ruan ◽  
Chonghua Wan ◽  
Jianfeng Tan ◽  
Bin Wu ◽  
...  

Abstract Background Chronic obstructive pulmonary disease (COPD) is one of the diseases with the highest morbidity and mortality globally. The Quality of Life Instrument for Chronic Diseases(QLICD)-COPD(V2.0) was designed to assess the health condition of patients with COPD. The objective of this study was to evaluate the quality of life (QOL) of patients, the influential clinical factors, and the relationships between QOL and clinical objective indicators. Methods Two hundred and sixty-one in-patients with COPD in the acute exacerbation stage were evaluated using the QLICD-COPD(V2.0) and data on clinical objective indicators were collected. The relationships between QOL and the clinical objective indicators were determined using canonical correlation analysis. Results The standardized scores for the patients in four domains, namely physical function, psychological function, social function, and a disease-specific module, were 49.00 ± 12.91, 59.89 ± 13.51, 68.59 ± 11.94, and 51.84 ± 13.58, respectively. The total score for the QOL of patients was 57.17 ± 10.26. Two pairs of canonical correlation variables were statistically significant (P < 0.05), with coefficients of 0.35 and 0.26. These variables respectively accounted for 45.8% and 33.8% of the variance. The levels of total protein, albumin, serum sodium, and alkaline phosphatase and the percentages of neutrophils and lymphocytes were correlated with the QOL. Conclusion During COPD treatment, clinicians should pay close attention to the levels of total protein, albumin, serum sodium, and alkaline phosphatase and the percentages of neutrophils and lymphocytes to improve the QOL of patients.


2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Xiyao Pang ◽  
Yanqiu Wang ◽  
Jintao Wu ◽  
Zhou Zhou ◽  
Tao Xu ◽  
...  

Yunnan Baiyao is a traditional Chinese herbal remedy that has long been used for its characteristics of wound healing, bone regeneration, and anti-inflammation. However, the effects of Yunnan Baiyao on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) and the potential mechanisms remain unclear. The aim of this study was to investigate the odonto/osteogenic differentiation effects of Yunnan Baiyao on SCAPs and the underlying mechanisms involved. SCAPs were isolated and cocultured with Yunnan Baiyao conditioned media. The proliferation ability was determined by cell counting kit 8 and flow cytometry. The differentiation capacity and the involvement of NF-κB pathway were investigated by alkaline phosphatase assay, alizarin red staining, immunofluorescence assay, real-time RT-PCR, and western blot analyses. Yunnan Baiyao conditioned medium at the concentration of 50 μg/mL upregulated alkaline phosphatase activity, induced more mineralized nodules, and increased the expression of odonto/osteogenic genes/proteins (e.g., OCN/OCN, OPN/OPN, OSX/OSX, RUNX2/RUNX2, ALP/ALP, COL-I/COL-I, DMP1, DSP/DSPP) of SCAPs. In addition, the expression of cytoplasmic phos-IκBα, phos-P65, and nuclear P65 was significantly increased in Yunnan Baiyao conditioned medium treated SCAPs in a time-dependent manner. Conversely, the differentiation of Yunnan Baiyao conditioned medium treated SCAPs was obviously inhibited when these stem cells were cocultured with the specific NF-κB inhibitor BMS345541. Yunnan Baiyao can promote the odonto/osteogenic differentiation of SCAPs via the NF-κB signaling pathway.


BMC Cancer ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Florence Joly ◽  
Claudia Lefeuvre-Plesse ◽  
Claire Garnier-Tixidre ◽  
Carole Helissey ◽  
Nathalie Menneveau ◽  
...  

Abstract Background Currently, oral targeted therapies are known to be effective and are frequently used to treat metastatic cancer patients, but fatigue is a frequently reported early side effect of these treatments. This fatigue may impact the patient’s treatment adherence and result in a negative impact on quality of life. Physical exercise significantly improved the general well-being and quality of life of advanced cancer patients. However, there is no specific physical activity program adapted for patients with advanced disease. Methods QUALIOR is a two-part, randomized, open-label, and multicenter with two arms phase II/III trial. Patients (phase II: n = 120; phase III: n = 312) with metastatic cancer (breast cancer, kidney cancer, lung cancer, and other cancers [including but not limited to colon cancer, melanoma, sarcoma, or hepatocarcinoma]) treated with a first- or second-line oral targeted therapy without chemotherapy will be included. Patients will be randomized (2:1) to a 3-month supervised home-based standardized physical activity program or to a recommended adapted physical activity (via a booklet). The primary objective of the phase II is to evaluate the feasibility of the supervised program. The primary objective of the phase III is the evaluation of the benefit of the supervised home-based program compare to the recommended program in terms of fatigue and quality of life at 3 months. The secondary objectives aim to evaluate the impact of the supervised program on fatigue over time, pain, physical capacities, psychosocial and cognitive functions, general quality of life, frequency of dose reduction and patients’ adherence to the targeted therapy, overall survival, and progression-free survival. This study will also evaluate the medico-economic impact of supervised program compared to the recommended adapted physical activity program. Discussion The aim of this study is to evaluate home-based physical exercise program for metastatic cancer patients treated with oral targeted therapies to help patients to cope with fatigue and improve quality of life. Trial registration This trial was registered in ClinicalTrials.gov since May 2017 (NCT03169075).


2018 ◽  
Vol 5 (2) ◽  
pp. 172 ◽  
Author(s):  
Kaori Yagasaki ◽  
Hiroko Komatsu ◽  
Kenzo Soejima ◽  
Katsuhiko Naoki ◽  
Ichiro Kawada ◽  
...  

2008 ◽  
Vol 41 (01) ◽  
pp. 08-14 ◽  
Author(s):  
Arash Zaminy ◽  
Iraj Ragerdi Kashani ◽  
Mohammad Barbarestani ◽  
Azim Hedayatpour ◽  
Reza Mahmoudi ◽  
...  

ABSTRACT Background: Osteogenesis driven by adipose-derived stem cells (ADSCs) is regulated by physiological and pathological factors. Accumulating evidence from in vitro and in vivo experiments suggests that melatonin may have an influence on bone formation. However, little is known about the effects of melatonin on osteogenesis, which thus remains to be elucidated. This study was performed to determine whether melatonin at physiological concentrations (0.01-10 nM) could affect the in vitro proliferation and osteogenic differentiation of rat ADSCs.Materials and Methods: ADSCs were isolated from the fat of adult rats. After cell expansion in culture media and through three passages, osteogenesis was induced in a monolayer culture using osteogenic medium with or without melatonin at physiological concentrations (0.01-10 nM). After four weeks, the cultures were examined for mineralization by Alizarin Red S and von Kossa staining and for alkaline phosphatase (ALP) activity using an ALP kit. Cell viability and apoptosis were also assayed by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTT) assay and flow cytometry, respectively.Results: The results indicated that at physiological concentrations, melatonin suppressed proliferation and differentiation of ADSCs. These data indicate that ADSCs exposed to melatonin, had a lower ALP activity in contrast to the cells exposed to osteogenic medium alone. Similarly, mineral deposition (calcium level) also decreased in the presence of melatonin. Flow cytometry confirmed that cell growth had decreased and that the numbers of apoptotic cells had increased.Conclusion: These results suggest that the physiological concentration of melatonin has a negative effect on ADSC osteogenesis.


Cancer ◽  
2016 ◽  
Vol 122 (9) ◽  
pp. 1461-1462 ◽  
Author(s):  
Andrea Tendas ◽  
Luca Cupelli ◽  
Maria Rita Mauroni ◽  
Fabio Sollazzo ◽  
Fabio Di Piazza ◽  
...  

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