scholarly journals Tetrachloroaurate (III)–induced Oxidation Increases Non-thermal Plasma-induced Oxidative Stress

Author(s):  
Yasumasa Okazaki ◽  
Kanako Sasaki ◽  
Nanami Ito ◽  
Hiromasa Tanaka ◽  
Ken-Ichiro Matsumoto ◽  
...  

Abstract Non-thermal plasma (NTP) devices have been explored for medical applications. NTP devices discharge electrons, positive ions, ultraviolet, and reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as the hydroxyl radical (●OH), singlet oxygen (1O2), superoxide (O2●−), hydrogen peroxide (H2O2), ozone, and nitric oxide, at near-physiological temperature. At preclinical stages or in human clinical trials, NTP promotes blood coagulation, eradication of bacterial, viral, and biofilm-related infections, wound healing, and cancer cell death. Here, we observed that ferric, vanadium, and gold(III) ions, measured by 2-thiobarbituric acid-reactive substances (TBARS) in combination with NTP exposure, significantly elevated lipid peroxidation. Using 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO) as a spin probe in electron paramagnetic resonance (EPR), we observed that tetrachloroaurate (III) yielded an M4PO-X spin adduct. Tetrachloroaurate-induced oxidation was attenuated efficiently by reduced (GSH) and oxidized glutathione (GSSG), while glycine (Gly) and L-glutamate (Glu), components of GSH, were ineffective. Furthermore, GSH and GSSG efficiently suppressed tetrachloroaurate-induced lipid peroxidation, while Gly and Glu were ineffective in suppressing TBARS elevation. These results indicate that tetrachloroaurate-induced oxidation is attenuated by GSH as well as GSSG. Further studies are warranted to elucidate the redox reactions between metal ions and biomolecules to advance the clinical application of NTP.

2005 ◽  
Vol 8 (1) ◽  
Author(s):  
Ulf Roland ◽  
Frank Holzer ◽  
Andreas Pöppl ◽  
Frank-Dieter Kopinke

AbstractIn order to evaluate the potential of the combination of non-thermal plasma (NTP) and in situ heterogeous catalysis (plasma catalysis) for the improvement of efficiency and selectivity towards total oxidation of organic pollutants, the impact of plasma processes inside the inner pore volume of porous materials was investigated by means of catalytic reactions and spectroscopy. Besides studying the conversion of organic model substances, electron paramagnetic resonance (EPR) spectroscopy was applied to detect both the formation of radical species by the NTP and the initiation of structural changes to the catalyst. The presence of short-lived oxidizing species and plasma effects in the inner pore volume of porous catalysts (alumina in this case) could be clearly shown by detecting a significant influence on the oxidation process and the formation of a paramagnetic site which can be correlated to an aluminum peroxide radical: Al-O-O. The relatively stable paramagnetic center (lifetime > 14 days) was formed by the NTP independently of the gas atmosphere, namely its oxygen content. The signal was not significantly affected by the application of reducing agents to the sample.


Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2195
Author(s):  
Carina Penzenstadler ◽  
Anna Zifko ◽  
Mohammad Jafarmadar ◽  
Janin Schulte ◽  
Joachim Struck ◽  
...  

Background aim: Reperfusion after hemorrhagic traumatic shock (HTS) is often associated with complications that are partly ascribed to the formation of reactive oxygen species (ROS). The aim of our study was to compare the effects of restrictive reperfusion (RR) to rapid full reperfusion (FR) on ROS formation and/or oxidative events. Materials and methods: Anesthetized male rats were randomly subjected to HTS followed by FR (75 mL/kg/h) or RR (30 mL/kg/h for 40 min, followed by 75 mL/kg/h) with Ringer’s solution (n = 8/group). Compartment-specific ROS formation was determined by infusion of ROS scavenger 1-hydroxy-3-carboxy-2,2,5,5-tetramethyl-pyrrolidine hydrochloride (CP-H) during resuscitation, followed by electron paramagnetic resonance spectroscopy. Sham-operated animals (n = 8) served as controls. The experiment was terminated 100 min post-shock. Results: Mean arterial pressure was significantly higher in the FR compared to the RR group during early reperfusion. Only RR animals, not FR animals, showed significantly higher ROS concentrations in erythrocytes (1951 ± 420 vs. 724 ± 75 AU) and in liver (474 ± 57 vs. 261 ± 21 AU) compared to sham controls. This was accompanied by elevated alanine aminotransferase and creatinine levels in RR animals compared to both shams and FR animals, while lipid peroxidation products (thiobarbituric acid reactive substances) were significantly increased only in the kidney in the FR group (p < 0.05). RR animals showed significantly higher plasma peroxiredoxin-4 values when compared to the FR group (20 ± 2 vs. 14 ± 0.5 RLU). Conclusion: Restrictive reperfusion after HTS is associated with increased ROS formation in erythrocytes and liver compared to sham controls. Moreover, the restrictive reperfusion is associated with a more pronounced injury to the liver and kidney, which is likely mediated by other than lipid peroxidation process and/or oxidative stress reactions.


1976 ◽  
Vol 37 (C7) ◽  
pp. C7-241-C7-246 ◽  
Author(s):  
J.P. VON DER WEID ◽  
L.C. SCAVARDA DO CARMO ◽  
R. R. DO SANTOS ◽  
B. KOILLER ◽  
S. COSTA RIBEIRO ◽  
...  

1964 ◽  
Vol 83 (7) ◽  
pp. 433-502 ◽  
Author(s):  
L.D. Bogomolova ◽  
V.N. Lazukin ◽  
I.V. Chepeleva

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