Wild Type and Variants of Sars-Cov-2 in Parisian Sewage: Dynamics in Raw Water and Fate in Wastewater Treatment Plants

Author(s):  
Melissa LOPEZ VIVEROS ◽  
Sam AZIMI ◽  
Elodie PICHON ◽  
Céline ROOSE-AMSALEG ◽  
Ariane BIZE ◽  
...  

Abstract The presence of SARS-CoV-2 RNA has been extensively reported at the influent of wastewater treatment plants (WWTPs) worldwide and its monitoring has been proposed as a potential surveillance tool to early alert of epidemic outbreaks. However, the fate of the SARS-CoV-2 RNA in the treatment process of WWTP has not been widely studied yet; therefore in this study, we aimed to evaluate the efficiency of treatment processes in reducing SARS-CoV-2 RNA levels in wastewater. The treatment process of three WWTPs of the Parisian area in France were monitored on six different weeks over a period of two months (from April 14th to June 9th 2021). SARS-CoV-2 RNA copies were detected using digital polymerase chain reaction (dPCR). Investigation on the presence of variants of concern (Del69-70E484 and L452R) was also performed. Additionally, SARS-CoV-2 RNA loads in the WWTPs influents were expressed as the viral charge per population equivalent and showed a good correlation with French public health indicators (incidence rate). SARS-CoV-2 RNA loads were notably reduced along the water treatment lines of the three WWTPs studied (2.5-3.4 log). Finally, very low SARS-CoV-2 RNA loads were detected in effluents (non-detected in over half of the samples) which indicated that the potential health risk of the release of wastewater effluents to the environment is probably insignificant, in the case of WWTPs enabling an efficient biological removal of nitrogen.

2006 ◽  
Vol 53 (11) ◽  
pp. 27-33 ◽  
Author(s):  
K. Komori ◽  
Y. Okayasu ◽  
M. Yasojima ◽  
Y. Suzuki ◽  
H. Tanaka

Nonylphenol (NP) is known to be a byproduct of nonylphenol ethoxylates (NPnEO) which are used as detergents in industry. It is important that not only NP but also NPnEO and their related substances are analysed when behaviour of NP in the wastewater treatment process is surveyed. NPnEO are biodegraded to shorter ethoxylate (EO) chain NPnEO or nonylphenol carboxylates (NPnEC) under aerobic conditions, and then biodegraded to NP under anaerobic conditions. NP is one of the suspected endocrine disruptors (ED). Moreover, shorter EO chain NPnEO has greater toxicity than longer EO chain NPnEO. We conducted a field survey of NP and its related substances in 20 wastewater treatment plants (WWTP). The concentrations (median) of NP and its related substances in the WWTPs' influent ranged from 0.1 to 8.3 μg/L, showing NP concentration as the same level as those previously reported. The reduction of the long EO chain NPnEO in the WWTPs was almost complete, while the removal efficiency for the short EO chain NPnEO was less significant than the long EO chain NPnEO, suggesting that the degradation rate of the short EO chain NPnEO was lower than that of the long EO chain NPnEO in the wastewater treatment processes.


Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 798
Author(s):  
Samendra P. Sherchan ◽  
Shalina Shahin ◽  
Jeenal Patel ◽  
Lauren M. Ward ◽  
Sarmila Tandukar ◽  
...  

In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A–F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April–July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.


2011 ◽  
Vol 1 (1) ◽  
pp. 37-56 ◽  
Author(s):  
Sílvia C. Oliveira ◽  
Marcos von Sperling

This article analyses the performance of 166 wastewater treatment plants operating in Brazil, comprising six different treatment processes: septic tank + anaerobic filter, facultative pond, anaerobic pond + facultative pond, activated sludge, UASB reactors alone, UASB reactors followed by post-treatment. The study evaluates and compares the observed effluent quality and the removal efficiencies in terms of BOD, COD, TSS, TN, TP and FC with typical values reported in the technical literature. In view of the large performance variability observed, the existence of a relationship between design/operational parameters and treatment performance was investigated. From the results obtained, no consistent relationship between loading rates and effluent quality was found. The influence of loading rates differed from plant to plant, and the effluent quality was dictated by several combined factors related to design and operation.


2010 ◽  
Vol 62 (10) ◽  
pp. 2450-2458 ◽  
Author(s):  
Angela Yu-Chen Lin ◽  
Cheng-Fan Lin ◽  
Yu-Ting Tsai ◽  
Hank Hui-Hsiang Lin ◽  
Jie Chen ◽  
...  

Pharmaceuticals and personal care products (PPCPs) constitute a class of chemicals of emerging concern due to the potential risks they pose to organisms and the environment, even at low concentrations (ng/L). Recent studies have found that PPCPs are not efficiently removed in secondary wastewater treatment plants (WWTPs). This study has: (1) simultaneously investigated the occurrence of sixty-one PPCPs using solid phase extraction and high-performance liquid chromatography-tandem mass spectrometry, (2) evaluated removal efficiencies of target PPCPs in six WWTPs that discharge effluents into major Taiwanese rivers, and lastly (3) examined matrix interference during analysis of target PPCPs in water samples. The twenty target PPCPs were chosen for their high detection frequencies, high influent concentrations, and stability during wastewater treatment processes. Caffeine and acetaminophen were detected at the highest concentrations (as high as 24,467 and 33,400 ng/L) and were effectively removed (both >96%); other PPCPs were detected in the high ng/L range but were not effectively removed. Matrix interference (by ion suppression or enhancement) during the analysis resulted in underestimation of the removal efficiencies of erythromycin-H2O, cefazolin, clarithromycin, ibuprofen, diclofenac, clofibric acid and gemfibrozil.


mSystems ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Pamela Y. Camejo ◽  
Ben O. Oyserman ◽  
Katherine D. McMahon ◽  
Daniel R. Noguera

“CandidatusAccumulibacter phosphatis” is widely found in full-scale wastewater treatment plants, where it has been identified as the key organism for biological removal of phosphorus. Since aeration can account for 50% of the energy use during wastewater treatment, microaerobic conditions for wastewater treatment have emerged as a cost-effective alternative to conventional biological nutrient removal processes. Our report provides strong genomics-based evidence not only that “Ca. Accumulibacter phosphatis” is the main organism contributing to phosphorus removal under microaerobic conditions but also that this organism simultaneously respires nitrate and oxygen in this environment, consequently removing nitrogen and phosphorus from the wastewater. Such activity could be harnessed in innovative designs for cost-effective and energy-efficient optimization of wastewater treatment systems.


2004 ◽  
Vol 4 (1) ◽  
pp. 23-33
Author(s):  
H. Ødegaard ◽  
Z. Liao ◽  
E. Melin ◽  
H. Helness

Many cities need to build compact wastewater treatment plants because of lack of land. This paper discusses compact treatment methods. An enhanced primary treatment process based on coarse media filtration is analysed. A high-rate secondary wastewater treatment process has specifically been investigated, consisting of a highly loaded moving bed biofilm reactor directly followed by a coagulation and floc separation step. The objective with this high-rate process is to meet secondary treatment effluent standards at a minimum use of chemicals, minimum sludge production and minimum footprint. It is demonstrated that the biofilm in the bioreactor mainly deals with the soluble organic matter while coagulation deals with the colloidal matter. The bioreactor may, therefore, be designed based on the soluble COD loading only, resulting in a very compact plant when a compact biomass/floc separation reactor (i.e. flotation or direct filtration) is used. The paper reports specifically on the coagulant choice in flotation and filter run time in direct filtration.


2019 ◽  
Vol 11 (23) ◽  
pp. 6658 ◽  
Author(s):  
I-Tae Kim

This study was conducted to secure the sustainability of biogas plants for generating resources from food waste (FW) leachates, which are prohibited from marine dumping and have been obligated to be completely treated on land since 2013 in South Korea. The aim of this study is to reduce the nitrogen load of the treatment process while producing bio-methanol using digested FW leachate diverted into wastewater treatment plants. By using biogas in conditions where methylobacter (M. marinus 88.2%) with strong tolerance to highly chlorinated FW leachate dominated, 3.82 mM of methanol production and 56.1% of total nitrogen (TN) removal were possible. Therefore, the proposed method can contribute to improving the treatment efficiency by accommodating twice the current carried-in FW leachate amount based on TN or by significantly reducing the nitrogen load in the subsequent wastewater treatment process. Moreover, the produced methanol can be an effective alternative for carbon source supply for denitrification in the subsequent process.


Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 720 ◽  
Author(s):  
Jin-Pil Kim ◽  
Dal Rae Jin ◽  
Wonseok Lee ◽  
Minhee Chae ◽  
Junwon Park

In this study, livestock wastewater treatment plants in South Korea were monitored to determine the characteristics of influent and effluent wastewater, containing four types of veterinary antibiotics (sulfamethazine, sulfathiazole, chlortetracycline, oxytetracycline), and the removal efficiencies of different treatment processes. Chlortetracycline had the highest average influent concentration (483.7 μg/L), followed by sulfamethazine (251.2 μg/L), sulfathiazole (230.8 μg/L) and oxytetracycline (25.7 μg/L), at five livestock wastewater treatment plants. Sulfathiazole had the highest average effluent concentration (28.2 μg/L), followed by sulfamethazine (20.8 μg/L) and chlortetracycline (11.5 μg/L), while no oxytetracycline was detected. For veterinary antibiotics in the wastewater, a removal efficiency of at least 90% was observed with five types of treatment processes, including a bio-ceramic sequencing batch reactor, liquid-phase flotation, membrane bioreactor, bioreactor plus ultrafiltration (BIOSUF) and bio best bacillus systems. Moreover, this study evaluated the removal efficiency via laboratory-scale experiments on the conventional contaminants, such as organic matter, nitrogen, phosphorus and veterinary antibiotics. This was done using the hydraulic retention time (HRT), under three temporal conditions (14 h, 18 h, 27 h), using the anaerobic–anoxic–oxic (A2O) process, in an attempt to assess the combined livestock wastewater treatment process where the livestock wastewater is treated until certain levels of water quality are achieved, and then the effluent is discharged to nearby sewage treatment plants for further treatment. The removal efficiencies of veterinary antibiotics, especially oxytetracycline and chlortetracycline, were 86.5–88.8% and 87.9–90.8%, respectively, exhibiting no significant differences under various HRT conditions. The removal efficiency of sulfamethazine was at least 20% higher at HRT = 27 h than at HRT = 14 h, indicating that sulfamethazine was efficiently removed in the A2O process with increased HRT. This study is expected to promote a comprehensive understanding of the behavior and removal of veterinary antibiotics in the livestock wastewater treatment plants of South Korea.


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