scholarly journals Multifarious Biomarker Approach Elucidate Stocking Density Mediated Stress, Modulates Growth Attributes in Cage Reared Rohu, Labeo Rohita (Hamilton)

2021 ◽  
Author(s):  
Himanshu Sekhar Swain ◽  
Basanta Kumar Das ◽  
Aurobinda Upadhyay ◽  
Mitesh Hiradas Ramteke ◽  
Vikas Kumar ◽  
...  
Keyword(s):  
Crude Protein ◽  
Labeo Rohita ◽  
Stocking Density ◽  
Inverse Correlation ◽  
Open Water ◽  
Fish Growth ◽  
Crowding Stress ◽  
Serum Protein Level ◽  
Growth Attributes ◽  
Ionic Imbalance

Abstract The present study was conducted for 240 days to evaluate the effects of stocking density based on growth attributes, digestive enzymes, muscular composition, biochemical and physiological responses of Labeo rohita fingerlings in tropical inland open water cages. L. rohita (30.35±1.08 g) were randomly distributed into three treatments, namely low stocking density, LSD (10 m− 3), medium stocking density, MSD (20 m− 3) and high stocking density, HSD (30 m− 3) in triplicates. Fish were fed twice daily with CIFRI CAGEGROW® floating feed (crude protein-28%, crude fat-4%). Fish growth and feed efficiency was higher (P<0.05) in LSD, however MSD registered higher yield. Amylase and protease activity reduced whereas lipase activity increased with increasing stocking density. Muscle crude protein and crude fat formed inverse correlation. The fillet quality deteriorated at higher stocking densities based on Muscle pH, drip loss and frozen leakage rate. The stress biomarkers level (glucose, cortisol, superoxide dismutase and catalase) increased in serum under crowding condition. Glutamate oxaloacetate transaminase and glutamate pyruvate transaminase in serum was significantly increased in HSD. Serum protein level decreased with increase in stocking densities. Body ionic imbalance (Na+, Cl− and K+) observed under crowding stress. Based on growth attributes and multiple biomarker responses, L. rohita @ 10 m− 3 found to be optimum density for inland open water cage culture.

scholarly journals Effect of Monoculture and Polyculture Systems on Growth and Production of Fishes in Seasonal Waterbodies of Haor Villages, Sunamganj District

2017 ◽  
Vol 9 (3) ◽  
pp. 307-316
Author(s):  
M. S. Ali ◽  
M. S. Islam ◽  
N. Begum ◽  
I. N. Suravi ◽  
M. Mia ◽  
...  
Keyword(s):  
Wheat Flour ◽  
Rice Bran ◽  
Oreochromis Niloticus ◽  
Labeo Rohita ◽  
Stocking Density ◽  
Total Biomass ◽  
Fish Production ◽  
Supplemental Feed ◽  
Final Weight

The study was conducted to assess the growth and production of Thai sarpunti (Puntius gonionotus), tilapia (Oreochromis niloticus) and rui (Labeo rohita) under monoculture and polyculture systems in seasonal waterbodies of haor environment. Selected waterbodies were divided into three treatments as T1 (sarpunti), T2 (sarpunti+tilapia) and T3 (sarpunti+tilapia+rui). Each treatment had three replicates and same stocking density (40,000 ha-1) of fishes was maintained in all treatments. Stocked fishes were fed with rice bran and wheat flour as supplemental feed at a decreasing rate of 10-5% of total biomass for twice daily. The highest final weight of sarpunti (122.32 g), tilapia (102.58 g) and rui (140.0 g) was obtained in T1, T2 and T3, respectively. Significantly higher production of sarpunti was secured in T1 (3,446.13 kg ha-1) than T2 (2,942.6 kg ha-1) and T3 (2,804.1 kg ha-1), and tilapia production was better in T2 (2,786.07 kg ha-1) than T3 (2,172.52 kg ha-1). Combined production of sarpunti, tilapia and rui was significantly (p<0.05) higher in T3 (8,341.41 kg ha-1) followed by T2 (5,728.67 kg ha-1) and T1 (3,446.13 kg ha-1). Therefore, polyculture practice of sarpunti, tilapia and rui may be suggested to disseminate at farmers’ level for boost up fish production.

scholarly journals Efficacy and suitability of whole wheat flour on the growth and survival of Rohu (Labeo rohita)

2019 ◽  
Vol 6 (2) ◽  
pp. 345-352
Author(s):  
Shovon Sarker ◽  
Md Fazle Rohani ◽  
Md Shafiqur Rahman ◽  
Md Sazzad Hossain
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Growth Performance ◽  
Wheat Flour ◽  
Labeo Rohita ◽  
Stocking Density ◽  
Quality Parameters ◽  
Whole Wheat Flour ◽  
Growth And Survival ◽  
Whole Wheat

The efficacy and suitability of dietary whole wheat flour supplementation was evaluated on the growth performance and survival of rohu (Labeo rohita). The study was conducted in 12 experimental aquaria at the Wet Laboratory, Department of Aquaculture, Bangladesh Agricultural University, Mymensingh. A total of 180 fingerlings of mean initial weight of 1.744±00 g were stocked at the same stocking density (268 fingerlings/m3). Four different treatments (T1, T2, T3 and T4) each with three replications were performed having different level of whole wheat flour such as 0% whole wheat flour (T1), 5% whole wheat flour (T2), 10% whole wheat flour (T3) and 15% whole wheat flour (T4). Feeds were supplied at 5% body weight twice daily in the morning at 9.00 am and in the afternoon at 5.00 pm throughout the study period. The rations were adjusted every week when new weights of the fish for various experimental aquariums were determined. At the end of the 63 days study period, the growth performance parameters such as mean final weight (g), weight gain (g), percent weight gain (%), specific growth rate (%/day) and the best feed utilization in terms of food conversion ratio were found highest at treatment 4 where fishes were fed with inclusion of 15% whole wheat flour and lowest was found at treatment 1 (fed with inclusion of 0% whole wheat flour). Water quality parameters were within the acceptable range and survival rate was found 100% in all treatment. Therefore, it can be concluded that treatment 4 (highest growth performance and survival rate of L. rohita with supplementation of 15% whole wheat flour containing diet) can be chosen by fish farmers for commercial culture of this species. Res. Agric., Livest. Fish.6(2): 345-352, August 2019

Antibacterial Activities of Antibacterial Proteins/Peptides Isolated from Organs and Mucus of Clarias Gariepinus Reared at High Stocking Density

2012 ◽  
Vol 455-456 ◽  
pp. 455-460 ◽  
Author(s):  
Xiao Mei Wang ◽  
Wei Dai ◽  
Ke Zhing Xing ◽  
Tian Jun Li ◽  
Xin Wang
Keyword(s):  
Molecular Weight ◽  
Antibacterial Activity ◽  
Crude Protein ◽  
Clarias Gariepinus ◽  
Gel Filtration ◽  
Stocking Density ◽  
Antibacterial Activities ◽  
Edwardsiella Tarda ◽  
Bacterial Strains ◽  
Sds Page

. Antibacterial proteins/peptides are important parts of the innate immune system in Clarias gariepinus. To examine potential antibacterial proteins/peptides in organs and mucus of C. gariepinus, crude protein/peptide extracts were isolated with ammonium sulfate precipitation from mucus, skin, gill, suprabranchial organ and intestine. Following further extraction using Sephadex G-50 gel filtration chromatography, the proteins/peptides associated with two absorption peaks (AP1 and AP2) were pooled, respectively, and assayed for their antibacterial activities against Escherichia coli, Aeromonas hydrophila and Edwardsiella tarda. The results showed that AP1 and AP2 from all the sampled tissues and mucus at concentration of 100 mg mL-1 exhibited antibacterial activity against the tested bacterial strains. Differences in antibacterial activity were observed among sample extracts. The protein profiles of AP1 obtained by Tricine-SDS-PAGE gel showed a broad range of peptides/proteins, and molecular weight of the mutual abundant peptide obtained was about 27 kDa. Antibacterial activity of AP2 extracted from intestine was due to peptide with molecular weight of 5.5 kDa.

scholarly journals Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos)

2020 ◽  
Vol 27 (2) ◽  
pp. 154
Author(s):  
Vivi Endar Herawati ◽  
Pinandoyo Pinandoyo ◽  
Seto Windarto ◽  
Putut Hariyadi ◽  
Johannes Hutabarat ◽  
...  
Keyword(s):  
Survival Rate ◽  
Fish Meal ◽  
Stocking Density ◽  
Feed Utilization ◽  
Fish Growth ◽  
Utilization Efficiency ◽  
Feed Consumption ◽  
Average Weight ◽  
Feed Formulation ◽  
Feed Utilization Efficiency

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17±4.58, FUE of 27.51±0.77, PER of 0.83±0.03%, and RGR of 2.34±0.10.

scholarly journals Effects of fish stocking density on growth and survival of Silver carp (Hypophthalmichthys molitrix) fry in Jashore, Bangladesh

2021 ◽  
Vol 33 (1) ◽  
pp. 65-74
Author(s):  
SK INJAMAMUL ISLAM ◽  
HASAN MAHMUD NOOR ◽  
MD. HABIBUR RAHMAN ◽  
MD. SAROWER-E-MAHFUJ ◽  
SYEDA MAKSUDA YEASMIN ◽  
...  
Keyword(s):  
Crude Protein ◽  
Feeding Rate ◽  
Average Length ◽  
Silver Carp ◽  
Stocking Density ◽  
Quality Parameters ◽  
Total Production ◽  
Hypophthalmichthys Molitrix ◽  
Growth And Survival ◽  
Acceptable Range

The experiment was operated in 9 consecutive weeks in nine earthen nursery ponds having the same area (0.09 acre) and depth (0.8 m). Seven days old fries of Silver carp (Hypophthalmichthys molitrix) were stocked at 4000, 5000 and 6000 fries/decimal in three treatments with three replications as T-1, T-2 and T-3, respectively to observe the growth and survival. At stocking, hatchlings were the same batch-rearing with an average length and weight of 0.44 ± 0.05 cm and 0.14 ± 0.05 mg, respectively. Wet mustered oil cake and commercial nursery feed containing 30% crude protein were given by two weeks interval, initially according to the bodyweight; the feeding rate was 14% in the first two weeks, gradually reduced to 12, 10 and 8%, respectively in the following week intervals. Water quality parameters and plankton sufficiency were observed every 15 days interval and was found within the acceptable range. Growth performance (6.458 g), survival rate of fingerlings (85.76%) and total production (22151g/decimal) were found highest in T-1 followed by T-2 and T-3. The study concluded that expect better production of fry up to fingerlings, proper stocking density should be maintained in pond fish culture in Jashore region of Bangladesh.

scholarly journals Transcriptomic analysis to elucidate the effects of high stocking density on grass carp (Ctenopharyngodon idella)

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yan He ◽  
Hongyan Yu ◽  
Honggang Zhao ◽  
Hua Zhu ◽  
Qingjing Zhang ◽  
...  
Keyword(s):  
Single Molecule ◽  
Grass Carp ◽  
Stocking Density ◽  
Alternative Polyadenylation ◽  
Fish Growth ◽  
Functional Enrichment ◽  
Ctenopharyngodon Idella ◽  
Smrt Sequencing ◽  
Nutrient Metabolism ◽  
New Genes

Abstract Background Grass carp (Ctenopharyngodon idella) is one of the most widely cultivated fishes in China. High stocking density can reportedly affect fish growth and immunity. Herein we performed PacBio long-read single-molecule real-time (SMRT) sequencing and Illumina RNA sequencing to evaluate the effects of high stocking density on grass carp transcriptome. Results SMRT sequencing led to the identification of 33,773 genes (14,946 known and 18,827 new genes). From the structure analysis, 8,009 genes were detected with alternative splicing events, 10,219 genes showed alternative polyadenylation sites and 15,521 long noncoding RNAs. Further, 1,235, 962, and 213 differentially expressed genes (DEGs) were identified in the intestine, muscle, and brain tissues, respectively. We performed functional enrichment analyses of DEGs, and they were identified to be significantly enriched in nutrient metabolism and immune function. The expression levels of several genes encoding apolipoproteins and activities of enzymes involved in carbohydrate enzymolysis were found to be upregulated in the high stocking density group, indicating that lipid metabolism and carbohydrate decomposition were accelerated. Besides, four isoforms of grass carp major histocompatibility complex class II antigen alpha and beta chains in the aforementioned three tissue was showed at least a 4-fold decrease. Conclusions The results suggesting that fish farmed at high stocking densities face issues associated with the metabolism and immune system. To conclude, our results emphasize the importance of maintaining reasonable density in grass carp aquaculture.

scholarly journals Effect of Stocking Density on Growth Performance and the Survival of Golden Mahseer, Tor putitora (Hamilton) Fry

2021 ◽  
pp. 47-54
Author(s):  
Md. Golam Sajed Riar ◽  
Nur- A Raushon ◽  
Sumit Kumer Paul
Keyword(s):  
Water Quality ◽  
Growth Performance ◽  
Crude Protein ◽  
Stocking Density ◽  
Experimental Period ◽  
Quality Parameters ◽  
The Other ◽  
Water Quality Parameters ◽  
Daily Weight Gain ◽  
Tor Putitora

Growth performance and survival of Tor putitora fry under different stocking densities were evaluated fry rearing system. The experimental period was 10 weeks from 31 December to 10 March 2019. The experiment was carried out in nine earthen ponds of 0.04 ha each under three treatments with three replications.  Thirty days old fry were stocked at the rate of 1.0X105/ha was designated as treatment- 1 (T1), 1.5X105/ha` as treatment- 2 (T2) and 2.0X105/ha as treatment- 3 (T3), respectively. Fry were fed with commercially available nursery feed containing 32% crude protein. It was observed that, lower stocking density showed highest daily weight gain in T1 (growth 0.066 ± 0.006 g/day) compared with higher stocking density in T2 (growth 0.044 ± 0.004 g/day) and T3 (growth 0.024 ± 0.003 g/day), respectively. It is also noticed that, the lower stocking showed the highest survival rate (79.66 ± 4.34 %) than the other two treatments (66.97±3.67 and 54.67± 3.12 %). The values of different water quality parameters were within the optimum ranges for the rearing of carp fry. Water quality parameters did not show significant variations in the experimental ponds under different stocking densities. Among these three stocking densities lower stocking density (T1) showed the best result compare with the other two higher stocking densities.

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