scholarly journals The Cross Talk Between ROS and NO in The Viability Declination of Cryopreserved-Pollen From Paeonia Lactiflora

Author(s):  
Ruifen Ren ◽  
Lingling Zhang ◽  
Hao Zhou ◽  
Xueru Jiang ◽  
Yan Liu

Abstract Reactive oxygen species (ROS) and nitric oxide (NO), as two common active molecules, are both involved in the changes of viability after liquid nitrogen (LN) stored, but the relationship between these two molecules has not been involved in plant cryopreservation up to now. In this study, the pollen of Paeonia lactiflora 'Fen Yu Nu' with significantly decreased viability after cryopreservation as materials, by studied the effects of the two regulators on each other and their biosynthesis and scavenging indexes, to explore the interaction between ROS and NO in pollen cryopreservation and its mechanism.The results showed that: the contents of ROS and NO increased significantly with the decrease of pollen viability after cryopreservation, and regulated ROS and NO content had a significant effect on post-LN pollen viability; the changes of ROS content positively regulated the endogenous NO content, and had significant effects on the expression level of NOS-like enzyme regulation gene CSU2 and its activity; while regulated NO had a positive effect on ROS content, and significantly affected the expression of NADPH oxidase and its regulatory gene RBOHJ, and also significantly affected catalase (CAT) and the substrate related to ascorbic acid (AsA)- glutathione (GSH) antioxidant cycle system. These results indicate that there was a positive interaction between ROS and NO in the pollen cryopreservation, NOS biosynthesis pathway was one of the ROS regulated NO pathways, NADPH oxidase, CAT and AsA-GSH antioxidant cycle system were the key sites for NO regulated ROS content.

2021 ◽  
Author(s):  
Ruifen Ren ◽  
Lingling Zhang ◽  
Hao Zhou ◽  
Xueru Jiang ◽  
Yan Liu

Abstract Reactive oxygen species (ROS) and nitric oxide (NO), two common active molecules, are both involved in changes in viability after liquid nitrogen (LN) storage, but the relationship between these two molecules has not been examined in plant cryopreservation until now. In this study, the pollen of Paeonia lactiflora 'Fen Yu Nu' with significantly decreased viability after cryopreservation was used as the material. We studied the effects of the two regulators on each other and their biosynthesis and scavenging indices to explore the interaction between ROS and NO in pollen cryopreservation and its mechanism. The results showed that the contents of ROS and NO increased significantly with the decrease of pollen viability after cryopreservation, and changes in the ROS and NO content had a significant effect on post-LN pollen viability. The ROS content positively regulated the endogenous NO content and had significant effects on the expression level of NOS-like enzyme regulation gene CSU2 and its activity. Down-regulated NO had a positive effect on the ROS content, and it significantly affected the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and its regulatory gene RBOHJ. It also significantly affected catalase (CAT) and substrates related to the ascorbic acid (AsA)-glutathione (GSH) antioxidant cycle system. These results indicate that there was a positive interaction between ROS and NO in pollen cryopreservation. The NOS biosynthesis pathway is one of the ROS-regulated NO pathways, and the NADPH oxidase, CAT and AsA-GSH antioxidant cycle systems are the key sites of regulation of the ROS content by NO.


2020 ◽  
Vol 5 (1) ◽  
pp. 52
Author(s):  
Sukirno Sukirno

Abstract This study aims to empirically challenge the moderation of Non-Performing Loans to the effect of Credit Distribution Rates on Profitability. The population of 81 bank companies listed on the Indonesia Stock Exchange in the period 2014-2018 and which met the criteria of the research sample (purposive sampling) were 22 companies. The research method uses survey methods with quantitative research approaches, the analytical tool used is moderation regression. This study concludes that the level of credit distribution has a significant positive effect on profitability and the existence of the problem loan variable is proven to be a moderating variable that weakens the relationship between the level of credit distribution and profitability.    


1997 ◽  
Vol 36 (4II) ◽  
pp. 855-862
Author(s):  
Tayyeb Shabir

Well-functioning financial markets can have a positive effect on economic growth by facilitating savings and more efficient allocation of capital. This paper characterises some of the recent theoretical developments that analyse the relationship between financial intermediation and economic growth and presents empirical estimates based on a model of the linkage between financially intermediated investment and growth for two separate groups of countries, developing and advanced. Empirical estimates for both groups suggest that financial intermediation through the efficiency of investment leads to a higher rate of growth per capita. The relevant coefficient estimates show a higher level of significance for the developing countries. This financial liberalisation in the form of deregulation and establishment and development of stock markets can be expected to lead to enhanced economic growth.


2018 ◽  
Vol 9 (2) ◽  
pp. 18-32
Author(s):  
Andy Mulyana ◽  
Devi Ayuni

This study aims to evaluate the role of satisfaction and commitment as mediating the effect of service quality on student loyalty as the user of Open University's Online Tutorial. Based on data analysis on student of Management program 229 as respondents with Structural Equation Modeling (SEM), this study found the relationship between: (1) positive influence of service quality to satisfaction and loyalty of students using tuton; (2) the positive effect of satisfaction on student loyalty using tuton; (3) satisfaction is a mediator between service quality and loyalty of students using tuton. Conversely, commitment has no positive effect on student loyalty as a tuton user. In addition, commitment does not mediates the effect of satisfaction on the loyalty of tuton users. Keywords: service quality, satisfaction, commitment, loyalty


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alicja Kowalczyk ◽  
Elżbieta Gałęska ◽  
Ewa Czerniawska-Piątkowska ◽  
Anna Szul ◽  
Leszek Hebda

AbstractThe aim of this study was to analyze the relationship between the concentration of hormones in the seminal plasma, the bull maintenance system in the insemination station, and the regularity of sperm donation and the response to the phantom (libido level). An additional goal was to determine whether there is a relationship between the hormonal profile in the blood, the sperm plasma, the oxidative and antioxidant profile in the blood of bulls and the biometry of their testicles and scrotum, as well as the quality of their sperm in both different seasons and intensities of reproductive use. For the study, 220 healthy and sexually mature Polish Holstein–Friesian bulls were used. They all had normal libido and were fed equally. The animals were grouped according to the scheme: young (16–20 month/n = 60) and old (26–30 month/n = 60) including: individually housed (n = 30) and group housed (n = 30) young, old individually housed (n = 30) and group housed (n = 30) (n total animals = 120); young animals donating semen once a week (every Thursday) (n = 25) and sporadically (once every two months on a random day of the week) (n = 25), old animals donating semen once a week (every Thursday) (n = 25 ) and sporadic donors (once every two months on a random day of the week) (n = 25) (n total animals = 100). When analyzing the results of this study, it should be stated that regular use has a positive effect on the secretion of sex hormones in bulls. Higher levels of testosterone and lower levels of estradiol and prostaglandins resulted in higher sexual performance, expressed by a stronger response to the phantom. The differences in favor of regular use were independent of the bull's age. The results of our research illustrate that the quality of semen and its freezing potential may depend on the season and frequency of its collection, as well as on the age of the males.


2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Xiang Yin ◽  
Zhiyi Meng ◽  
Xin Yi ◽  
Yong Wang ◽  
Xia Hua

AbstractChina has made great efforts to alleviate poverty in rural ethnic minority areas and targeted achieving the poverty-alleviation task by the end of 2020. Aba, Ganzi, and Liangshan, three of the poorest ethnic prefectures in Sichuan Province, Southwest China, have all implemented “Internet+” tactics since 2013, which have had the positive effect of increasing family revenues by improving communication infrastructure and encouraging the large-scale use of e-commerce. This paper aims to comprehensively investigate whether “Internet+” tactics play a key role in poverty alleviation in Sichuan’s rural ethnic minority areas and to propose further measures to enhance the efficiency of e-commerce practice. To this end, we conduct an analysis using the framework of classic growth theory and use panel data from 2000 to 2018 to examine the relationship between Communication Infrastructure Investment (CII) and a set of poverty-alleviation indicators, including local GDP growth rate (LGGR), local government revenue (LGR), and per-capita income of residents (PCIR). The results indicate that strengthening CII improves the PCIR and local economic growth, playing a key role in poverty alleviation. However, the stimulation of CII on LGGR and LGR wanes as time passes. More financial and technical actions will be needed to improve the efficiency and quality of current strategies for sustainable development in those areas.


2021 ◽  
Vol 13 (4) ◽  
pp. 1851
Author(s):  
Alexis Poulhès ◽  
Angèle Brachet

Mid-sized cities are usually considered in the literature to be shrinking cities. Some policies promote right-sizing and others promote revitalization. The relationship between land-use planning and mobility having been established, the present research issue is focused on whether a policy of revitalizing the centers of mid-sized cities is favorable to low-carbon mobility. Our study investigates commuting trips through two indicators: commuting trip distance and car modal share. The increase in total population, the increase in the number of jobs per resident, the decrease in the unemployment rate, the increase in the rate of executives, the increase in the rate of working people in the population and the decrease in the residential vacancy rate all come from the censuses of 2006 and 2016. Statistical models based on individuals in 113 mid-sized cities, in which sociodemographic variables are introduced, show that at the level of agglomerations, no indicator has a simultaneously positive effect in the center and in the urban periphery. No indicator is entirely positive or negative on GHG emissions from commuting trips. While the increase in GHG emissions from commuting trips between 2006 and 2016 is significant in mid-sized cities (18%), a shift toward shrinking city centers is insufficient to change this trajectory.


2021 ◽  
Vol 13 (7) ◽  
pp. 3986
Author(s):  
Jun-Chul Ha ◽  
Jun-Woo Lee ◽  
Jee Young Seong

In a rapidly changing business environment, the entrepreneurship of top management is essential for the survival and sustainable development of the enterprise. Building on the view of the strategic choice theory, this study identifies the relationship between entrepreneurship, market-oriented culture, and work engagement. Data were collected from 493 employees regularly working in small and medium-sized firms in South Korea. The results of this study indicate: (1) entrepreneurship (consisting of innovation, proactiveness, and risk-taking) has a significant positive influence on market-oriented culture, (2) entrepreneurship positively affects work engagement, (3) market-oriented culture has a significant positive effect on work engagement, (4) the effects of innovation and proactiveness on work engagement are significant, controlling for market-oriented culture, showing the partial mediating effect of market-oriented culture on work engagement, and (5) CEO trust moderates the relationship between risk-taking and work engagement. Theoretical and practical implications are suggested.


Author(s):  
Danai Christopoulou ◽  
Nikolaos Papageorgiadis ◽  
Chengang Wang ◽  
Georgios Magkonis

AbstractWe study the role of the strength of Intellectual Property Rights (IPR) law protection and enforcement in influencing horizontal productivity spillovers from inward FDI to domestic firms in host countries. While most WTO countries adopted strong IPR legislation due to exogenous pressure resulting from the signing of the Trade-Related Aspects of IPR (TRIPS) agreement, public IPR enforcement strength continues to vary significantly between countries. We meta-analyse 49 studies and find that public IPR enforcement strength has a direct positive effect on horizontal productivity spillovers from inward FDI to domestic firms and a negative moderating effect on the relationship between IPR law protection strength and horizontal productivity spillovers from inward FDI to domestic firms.


2020 ◽  
Vol 12 (4) ◽  
pp. 1366
Author(s):  
Julio C. Acosta-Prado ◽  
Oscar H. López-Montoya ◽  
Carlos Sanchís-Pedregosa ◽  
Ulpiano J. Vázquez-Martínez

The literature suggests that innovation allows organizations to reach a desirable level of sustainability. There is evidence to support the role of knowledge management (KM) as well as management capability (MC) in producing a sustainable approach at organizations. Furthermore, organizations commonly achieve sustainable practices through corporate social responsibility (CSR). In particular, the health sector is increasingly implementing CSR strategies, although with a narrow understanding of the factors to success. Hence, trends lead to asymmetric growth between organizations. This study aims to examine the mediating role of KM in the relationship between MC and innovative performance (IP) in 331 Health Provider Institutions (HPIs). The research reflective model was assessed through Partial Least Squares Structural Equation Modeling (PLS-SEM). According to the results, MC has a positive effect on IP, MC has a positive effect on KM, and KM has a positive effect on IP. Likewise, KM significantly mediates the relationship between MC and IP. Our findings support the importance of KM in addressing MCs in HPIs as it enables innovative practices to address CSR goals to achieve a sustainable impact. Moreover, this study contributes by expanding KM to contexts that are not usually studied, such as health in a South American country.


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