scholarly journals Membrane Lipid Composition and Heat Tolerance in Cool-season Turfgrasses, including a Hybrid Bluegrass

2009 ◽  
Vol 134 (5) ◽  
pp. 511-520 ◽  
Author(s):  
Kemin Su ◽  
Dale J. Bremer ◽  
Richard Jeannotte ◽  
Ruth Welti ◽  
Celeste Yang

Cool-season turfgrasses may experience heat stress during summer. Hybrid bluegrasses (HBGs), crosses between kentucky bluegrass [KBG (Poa pratensis L.)] and native texas bluegrass (Poa arachnifera Torr.), have improved heat tolerance but the mechanisms of heat tolerance are poorly understood. Our objectives were to quantitatively profile membrane lipid molecular species in three cool-season turfgrasses exposed to optimal (22/15 °C, 14/10 h light/dark) and supra-optimal temperatures (35/25 °C and 40/30 °C, 14/10 h light/dark). Grasses included a low heat-tolerant tall fescue [TF (Festuca arundinacea Schreb. ‘Dynasty’)], a mid-heat–tolerant KBG (‘Apollo’), and a heat-tolerant HBG (‘Thermal Blue’). At high temperature, glycolipid digalactosyldiacylglycerol (DGDG) in HBG was 12% and 16% greater than in KBG and TF, respectively, and the ratio DGDG to monogalactosyldiacylglycerol was 19% and 44% greater in HBG than in KBG and TF, respectively. Greater heat tolerance in HBG and KBG was associated with higher contents of phosphatidylethanolamine and phosphatidylglycerol, and with reduced overall unsaturation compared with TF. Overall, 20 lipid molecular species were present in greater amounts and another 20 species in lesser amounts in HBG and KBG than in TF. Results suggest 40 membrane lipid molecules are potential biomarkers for heat tolerance and that compositional changes in membrane lipids in response to heat contribute to differences in heat tolerance among cool-season grasses.

2017 ◽  
Vol 2 (3) ◽  
pp. 162-170
Author(s):  
Kenneth Lynn Diesburg ◽  
Ronald F. Krausz

This research was conducted to determine the degree of success, by month, in seeding establishment of tall fescue (Festuca arundinacea Schreb., Kentucky bluegrass (Poa pratensis L.), Bermudagrass (Cynodon dactylon [L.] Pers. var. dactylon), and zoysiagrass (Zoysia japonica Steud.) at two locations in the moist, Midwest, continental transition zone on a prepared seed bed without irrigation or cover. The four species were planted every month of the year starting in September 2005. Starter fertilizer and siduron were applied the same day as seeding with no subsequent management except mowing. Percent cover of living turfgrass was recorded in each of 24 months after seeding. Tall fescue (80%) and Bermudagrass (73%) provided the best percent cover over all planting dates. Kentucky bluegrass provided 65% and zoysiagrass 24% cover. The cool-season grasses performed best in the July-to-March plantings; tall fescue 88% and Kentucky bluegrass 72%. Bermudagrass (94%) established best in the January-to-April plantings, while Zoysiagrass (32%) established best in the November-to-March plantings. Germination and seedling survival after germination of all species were inhibited by limited moisture during summer. The warm-season grasses were further limited by winter kill in the August, September, and October seedings. These results emphasize the risk in spring-seeding as well as the value in dormant-seeding of both warm- and cool-season turfgrasses for low-input, nonirrigated establishment.


2001 ◽  
Vol 11 (1) ◽  
pp. 152a
Author(s):  
Zachary J. Reicher ◽  
Clark S. Throssell ◽  
Daniel V. Weisenberger

Little documentation exists on the success of seeding cool-season turf-grasses in the late fall, winter and spring. The objectives of these two studies were to document the success of seeding Kentucky bluegrass (Poa pratensis L.), perennial ryegrass (Lolium perenne L.), and tall fescue (Festuca arundinacea Schreb.) at less-than-optimum times of the year, and to determine if N and P fertilizer requirements vary with seeding date of Kentucky bluegrass. `Ram I' Kentucky bluegrass, `Fiesta' perennial ryegrass, and `Mustang' tall fescue were seeded on 1 Sept., 1 Oct., 1 Nov., 1 Dec., 1 Mar., 1 Apr., and 1 May ± 2 days beginning in 1989 and 1990. As expected, the September seeding date produced the best establishment, regardless of species. Dormant-seeding Kentucky bluegrass and tall fescue in November, December, or March reduced the establishment time compared with seeding in April or May. Seeding perennial ryegrass in November, December, or March may not be justified because of winterkill potential. To determine the effect of starter fertilizer on seedings made at different times of the year, `Ram 1' Kentucky bluegrass was seeded 1 Sept., 1 Nov., 1 Mar., and 1 May ± 2 days in 1989 and 1990, and the seedbed was fertilized with all combinations of rates of N (0, 24, and 48 kg·ha-1) and P (0, 21, and 42 kg·ha-1). Fertilizer rate had no effect on establishment regardless of seeding date, possibly because of the fertile soil on the experimental site.


HortScience ◽  
2000 ◽  
Vol 35 (6) ◽  
pp. 1166-1169 ◽  
Author(s):  
Zachary J. Reicher ◽  
Clark S. Throssell ◽  
Daniel V. Weisenberger

Little documentation exists on the success of seeding cool-season turfgrasses in the late fall, winter and spring. The objectives of these two studies were to document the success of seeding Kentucky bluegrass (Poa pratensis L.), perennial ryegrass (Lolium perenne L.), and tall fescue (Festuca arundinacea Schreb.) at less-than-optimum times of the year, and to determine if N and P fertilizer requirements vary with seeding date of Kentucky bluegrass. `Ram I' Kentucky bluegrass, `Fiesta' perennial ryegrass, and `Mustang' tall fescue were seeded on 1 Sept., 1 Oct., 1 Nov., 1 Dec., 1 Mar., 1 Apr., and 1 May ± 2 days beginning in 1989 and 1990. As expected, the September seeding date produced the best establishment, regardless of species. Dormant-seeding Kentucky bluegrass and tall fescue in November, December, or March reduced the establishment time compared with seeding in April or May. Seeding perennial ryegrass in November, December, or March may not be justified because of winterkill potential. To determine the effect of starter fertilizer on seedings made at different times of the year, `Ram 1' Kentucky bluegrass was seeded 1 Sept., 1 Nov., 1 Mar., and 1 May ± 2 days in 1989 and 1990, and the seedbed was fertilized with all combinations of rates of N (0, 24, and 48 kg·ha-1) and P (0, 21, and 42 kg·ha-1). Fertilizer rate had no effect on establishment regardless of seeding date, possibly because of the fertile soil on the experimental site.


2010 ◽  
Vol 135 (2) ◽  
pp. 116-124 ◽  
Author(s):  
Yali He ◽  
Bingru Huang

Understanding antioxidant mechanisms for heat stress is important for improving heat tolerance in cool-season plant species. The objective of this study was to identify antioxidant enzymes associated with cultivar variations in heat tolerance in kentucky bluegrass (Poa pratensis) by comparing heat responses of activity and isoforms of antioxidant enzymes in two cultivars contrasting in heat tolerance. Plants of heat-tolerant ‘Eagleton’ and heat-sensitive ‘Brilliant’ were exposed to 20 °C (control) or 40 °C (heat stress) for 28 days in growth chambers. Chlorophyll (Chl) a content remained unchanged and Chl b content increased in ‘Eagleton’, while both of them decreased in ‘Brilliant’, and by 28 days, ‘Eagleton’ had significantly higher Chl a and b content than ‘Brilliant’. The activities of superoxide dismutase (SOD) were significantly higher in ‘Eagleton’ than in ‘Brilliant’ by 28 days of heat stress. An isozyme SOD2 was induced early during heat stress in ‘Eagleton’, while isozyme SOD3 degraded, to a lesser extent in ‘Eagleton’ than in ‘Brilliant’. Catalase (CAT) activity significantly increased in ‘Brilliant’ but remained constant in ‘Eagleton’, and ‘Brilliant’ had a significantly higher CAT activity and isozyme CAT1 than ‘Eagleton’ during heat stress. Significant increases in ascorbate peroxidase (APX) activities occurred under heat stress, to a greater extent in ‘Eagleton’, whereas isozymes did not exhibit difference between cultivars. Guaiacol-peroxidase (POD) activity declined during heat stress in both cultivars. The intensity of POD isozymes in ‘Brilliant’ remained constant, while ‘Eagleton’ showed a transient increases in POD1 at 7 days of heat stress. Our results indicated that antioxidant defense mechanisms for heat tolerance in kentucky bluegrass could be mainly associated with changes in activity and forms of isozymes of SOD for O2 scavenging and APX activity for H2O2 scavenging under heat stress.


HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 650-653 ◽  
Author(s):  
Mark G. Lefsrud ◽  
John C. Sorochan ◽  
Dean A. Kopsell ◽  
J. Scott McElroy

Heat-tolerant bluegrass varieties were developed to resist dormancy and retain pigmentation during heat stress events. The objective of this study was to investigate the influence of grass species, nitrogen (N) fertilization, and seasonality on the accumulation patterns of lutein, β-carotene, and chlorophyll a and b in the leaf tissues of turfgrass. The heat-tolerant bluegrass cultivars Dura Blue and Thermal Blue (Poa pratensis L. × Poa arachnifera Torr.), Apollo kentucky bluegrass (Poa pratensis L.), and Kentucky 31 tall fescue (Festuca arundinacea Schreb.) were compared for the accumulation of plant pigments. Evaluations were conducted over 2 consecutive years (Years 4 and 5 after establishment) during two different seasons (spring and summer) and under varying N fertilization. Fertilizer applications of 5, 14, and 27 g N/m2/year resulted in a significant positive correlation for the accumulation of leaf blade lutein and chlorophyll a and b, but not for β-carotene. The accumulation of the four measured plant pigments among the grasses was significantly different with ‘Apollo’ having the largest concentration of pigments followed by ‘Dura Blue’, ‘Thermal Blue’, and finally ‘Kentucky 31’. Specifically, when comparing the cultivars Apollo and Kentucky 31, the pigment levels decreased 27%, 26%, 26%, and 23% for lutein, β-carotene, and chlorophyll a and b, respectively. The interesting observation of the analysis of the grass pigment concentrations was that the least reported heat-tolerant cultivar in our study (‘Apollo’) had the largest measured pigment concentrations.


1997 ◽  
Vol 77 (1) ◽  
pp. 75-80 ◽  
Author(s):  
R. L. Hockensmith ◽  
C. C. Sheaffer ◽  
G. C. Marten ◽  
J. L. Halgerson

Kentucky bluegrass (Poa pratensis L.) is an important constituent of many permanent pastures in the northern United States and is an important source of livestock feed, but there is a paucity of information on its forage quality. The objective of this research was to assess changes with maturity in forage quality of whole herbage, leaves, and stems of Kentucky bluegrass compared with other important cool-season forage grasses. Kentucky bluegrass, orchardgrass (Dactylis glomerata L.), reed canarygrass (Phalaris arundinaceae L.), smooth bromegrass (Bromus inermis Leyss), timothy (Phleum pratense L.), and tall fescue (Festuca arundinacea Schreb) were sampled weekly beginning in mid-May until each species reached milk stage. Kentucky bluegrass had the highest or was among the grasses with the highest average leaf percentage, and leaf, stem, and whole herbage neutral detergent fiber (NDF) and acid detergent lignin (ADL) concentrations; and lowest average whole herbage, leaf, and stem in vitro digestible dry matter (IVDDM) concentrations compared with tall growing species. Kentucky bluegrass also had the slowest rates of change in leaf and stem concentration and in whole herbage IVDDM and NDF concentrations with maturity. Leaf concentration was negatively correlated with herbage NDF and ADL concentration, but was not correlated with herbage IVDDM and CP concentration. Relative to tall growing cool season grasses, Kentucky bluegrass is leafier, but it has poorer forage digestibility. Key words: Poa pratense L., forage quality, perennial grass, leaves, stems


2021 ◽  
pp. 1-12
Author(s):  
Trent M. Tate ◽  
Stacy A. Bonos ◽  
William A. Meyer

Fine fescues (Festuca sp.) are a group of species that require fewer inputs, such as fertilizer, than other cool-season species managed for turf. They are adapted to infertile, acidic soils; shade; and drought. One area that poses additional challenges is the lack of weed control options for fine fescues during establishment from seed. Mesotrione is a herbicide that provides preemergence control of many broadleaf and grassy weeds, such as annual bluegrass (Poa annua), but is currently not labeled for use in fine fescues at seeding. The objectives of this research were 1) to use a recurrent selection technique to develop mesotrione-tolerant chewings fescue (Festuca rubra ssp. commutata), hard fescue (Festuca brevipila), and strong creeping red fescue (F. rubra spp. rubra); and 2) to conduct field trials to compare the new selections to commercially available cultivars and experimental lines not selected for tolerance to mesotrione. Progress was made after each of the three generations of recurrent selection. The top statistical grouping of entries for injury following application of mesotrione at the 8-oz/acre rate included all the third-generation (G3) hard fescues, all the G3 chewings fescues, and the G3 strong creeping red fescue STB1 Composite. After three generations, selections of hard, chewings, and strong creeping red fescues had equivalent or better tolerance to mesotrione than tall fescue (Festuca arundinacea) and kentucky bluegrass (Poa pratensis) cultivars, which are on the label for safe use at seeding. These new selections would provide turf managers an option to control weeds using mesotrione during seedling establishment of fine fescues.


HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 509-513 ◽  
Author(s):  
Peter H. Dernoeden ◽  
John E. Kaminski ◽  
Jinmin Fu

Creeping bentgrass (Agrostis stolonifera L.; CBG) is a common weed in home lawns and golf course roughs in many regions of the United States. Currently, no herbicides are registered for selective control of CBG in cool-season grasses. The objective of this field study was to evaluate the ability of mesotrione and triclopyr ester to selectively remove CBG from Kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.). Mesotrione (0.14 and 0.21 kg·ha−1 a.i.) and triclopyr ester (0.56 and 1.12 kg·ha−1 a.i.) were applied on a 2-week interval two, three, or four times in Connecticut and Maryland in 2005, and three or four times in Maryland in 2006. Two applications of mesotrione at 0.21 kg·ha−1 a.i. provided marginally acceptable CBG control, but three or four applications at 0.14 or 0.21 kg·ha−1 a.i. provided excellent CBG control. Mesotrione elicited little or no injury to Kentucky bluegrass, but generally caused objectionable injury in tall fescue for about 7 to 14 d after each application. Triclopyr applied at 0.56 kg·ha−1 a.i. reduced CBG cover, but the level of control generally was unacceptable, regardless of application frequency. Three or four applications of triclopyr (1.12 kg·ha−1 a.i.) effectively controlled CBG in Connecticut in 2005 and Maryland in 2006. Triclopyr caused no visual injury to tall fescue, regardless of rate or application frequency. Four triclopyr applications to Kentucky bluegrass, however, were phytotoxic and reduced stand density, especially at the high rate (1.12 kg·ha−1 a.i.). Three summer applications of mesotrione (0.14 kg·ha−1 a.i.) or triclopyr (1.12 kg·ha−1 a.i.) provided the best combination of turfgrass safety and CBG control. Chemical names used: [(3,5,6-trichloro-2-pyridinyl)oxy]acetic acid (triclopyr ester); 2-[4-(methylsulfonyl)-2-nitrobenzoyl]-1,3-cyclohexanedione (mesotrione)


HortScience ◽  
2015 ◽  
Vol 50 (4) ◽  
pp. 615-620 ◽  
Author(s):  
Bret Sparks ◽  
Gregg Munshaw ◽  
David Williams ◽  
Michael Barrett ◽  
Jeffrey Beasley ◽  
...  

Managed turfgrass species require frequent inputs to maintain an acceptable level of density and appearance. Among these inputs, the N supply is often the most limiting input in terms of growth and development of the turfgrass stand. However, N fertilization has been linked to nonpoint source (NPS) pollution of groundwater and natural water bodies. White clover (WC), which would provide N in mixed turfgrass swards, could help reduce NPS pollution from N fertilization of turf. To test the feasibility of introducing WC into existing turf, a field study was designed to determine the best method of incorporating WC in mature stands of two cool-season grasses. Two varieties of WC, ‘Dutch White’ (DW), and ‘Microclover’ (MC), were sown (24.4 kg·ha−1) into existing stands of kentucky bluegrass (KBG) (Poa pratensis L.) and tall fescue (TF) (Festuca arundinacea Schreb.). Establishment techniques tested included core aeration (CA), scalping (SC), and vertical mowing (VM) compared with direct sowing into the turfgrass stand. Establishment treatments were performed in April, July, and October of 2012–13 to examine for any seasonal timing effect on establishment. No significant difference in plant numbers (individual clover plants per square meter) was found between WC varieties among planting dates and techniques. The SC treatment resulted in the highest individual clover plant numbers. However, turfgrass recovery was significantly slower from the SC treatment than all other treatments. The summer planting date yielded the highest WC plant numbers. Recovery of the turfgrass from all preplanting treatments was also highest at the spring and summer planting dates.


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