scholarly journals Biopotential of collagen-containing hydrolysates obtained from fish raw materials for protein sports nutrition

Author(s):  
Yu. O. Nekrasova ◽  
O. Ya. Mezenova ◽  
J. Th. Moersel

Low-molecular peptides of collagen origin are of crucial importance for the body metabolism. This work aims to substantiate the use of the hydrolysates of collagen-containing fish raw materials (scale) for protein sports nutrition. Sublimated low-molecular weight peptide water-soluble and dried water-insoluble protein-mineral supplements from the sardine scale were obtained by enzymatic thermal hydrolysis. The biopotential of scale and auxiliary food additives (apple pomace, flaxseed cake, pine nuts) was investigated using standard methods. The protein content and amino acid composition of the peptide supplement and the mineral composition of the protein-mineral supplement were analysed; their high biopotential according to the above criteria was shown. The peptide supplement contains all essential amino acids having a biological value of 59.9%. The protein-mineral supplement contains no tryptophan but a high amount of calcium (22.2 g/100 g) and phosphorus (12.0 g/100 g). The high content of functional food ingredients (protein, pectin, cellulose, polyphenols, minerals, vitamin C) in auxiliary vegetable raw materials was shown. The shape of a sports nutrition product (chocolate glazed protein bar) was substantiated. The basic recipe of a 60 g protein bar having the required protein content, a given structure and high consumer properties was established. Having a protein content of 23.5%, the bar provides a 24.1% calorific value, which corresponds to the "protein-rich food" for sports nutrition according to GOST 34006-2016. The biological protein value in the bar increased relative to the peptide supplement by 12% and amounted to 71.38%. The developed protein sports nutrition bar is dietary balanced by the content of amino acids (isoleucine, leucine, valine, methionine and cystine, phenylalanine and tyrosine, tryptophan, lysine, threonine, alanine, arginine, histidine, glycine, carnosine, taurine, ornithine, citrulline), fibre, polyphenols, calcium and phosphorus and is recommended for athletes and people having an active lifestyle.

Author(s):  
Kalu E. Chinedu ◽  
Eugene N. Onyeike ◽  
Catherine C. Ikewuchi

Aim: The quantitative nutrient composition of leaves of Brillantaisia guianensis P. beuv was investigated. Source and Identification of Sample: The leaves of B. guianensis used in this study were harvested fresh from Ude plantation in Okon Aku, Ohafia Local Government Area of Abia State, Nigeria. They were identified by a Taxonomist (Dr Edwin Wosu) in the Herbarium Unit of the Department of Plant Science and Biotechnology, University of Port Harcourt.  Study Design: The study was designed, using standard methods, to determine the proximate, amino acids, minerals and vitamins composition of the leaves.  Results: The wet leaves were high in moisture (88.4%) while the dried sample had practically no moisture, indicating that the wet leaves could easily be prone to microbial spoilage due to high water activity. Drying increased protein level from 3.50 to 28.0% and ash from 2.36 to 27.7% among other parameters. The low calorific values for wet sample (16.1 kcal/100 g) and dry sample (152.3 kcal/100 g sample) showed that the leaves are poor energy-giving foods. However, the leaves contain good amino acid profile especially the essential amino acids (valine, threonine, isoleucine, leucine, lysine, methionine, phenylalanine and tryptophan). The nutrient mineral (Na, Ca, Mg, K, Se, P, Fe) levels were high to supply the World Health Organization recommended daily allowance for adults and children. The sample contains appreciable amount of fats and water -soluble vitamins required for growth.   Conclusion: The results show that the leaves are good sources of essential and non-essential amino acids, minerals and vitamins adequate to meet the required daily allowance for nourishment of the body.


Author(s):  
Maria Evgenievna Tsibizova

In the context of the organization of proper nutrition of the population of Russia, regardless of belonging to any physiological group, taking into account the eating behavior of a modern person, the research has been carried out to expand the range of fish pastes by modifying their recipes. The goal has been achieved by combining raw materials of different origins and reducing the mass fraction of table salt. Experimental studies were carried out to substantiate the recipe compositions of pastes based on grass carp meat, the optimal proportion of calcium-phosphate mineral supplements was defined, a comparative analysis of organoleptic indicators of quality, chemical composition and energy value of the obtained products was conducted. It has been stated that the introduction of 2.5% of phosphate-calcium food additives into paste recipes No. 4 and 5 did not adversely affect the organoleptic quality indicators. The pastes produced according to recipes No. 4 and No. 5, which include grass carp meat (48%), chicken liver (10%), vegetable components (17%), calcium-phosphate mineral supplement (2,5%) and flavoring substances, can be recommended for mass nutrition of the population of Russia. The ratio of these components provides the high organoleptic properties and improved nutritional value. The inclusion of fish pastes enriched with vegetable components, chicken by-products, phosphate-calcium mineral supplements, differing in chemical composition, in the diet allows balancing the diet for proteins, fats, amino acids, vitamins, macro- and micronutrients and preventing from eating disorders and, in the result, reducing morbidity of population in the Russian Federation.


2021 ◽  
Vol 4 (1) ◽  
pp. 56-72
Author(s):  
Mykhailo Peresichnyi ◽  
Svitlana Peresichna ◽  
Anna Sobko

The topicality. To date, the structure of the population’s diet has significant deviations from the formula of a balanced diet in terms of protein intake, including sulfur-containing, which causes the formation of risk factors for the development alimentary and alimentary-dependent diseases. For the prevention of diseases caused by protein deficiency, it is promising to increase its content in vegetable dishes due to the integrated use of raw materials and dietary supplements of high biological value. It is advisable to use food combinatorics in the innovative technologies development for vegetable dishes of improved amino acid composition. The purpose of the study is food combinatorics of amino acid composition of potato croquettes with soy flour, wheat germ; natural shrimp powder “Rieber Food Ingredients” and lentil-spirulina filling in terms of content and balance of essential and substitute amino acids and the degree of their assimilation by the human body. Research methods. Physicochemical, mathematical and statistical methods of experimental data processing with the use of information technologies, methods of ion exchange liquid column chromatography and qualimetric methods have been used. Results. Research is aimed at the use of food combinatorics and scientific substantiation of the amino acid composition of potato croquettes with the use of protein vegetable and non-fish water raw materials and dietary supplements. As a result of scientific researches the food combinatorics at development of technology of vegetable dishes has been carried out, the amino acid structure has been proved and experimentally generalized, the amino acid score and balance of sulfur-containing amino acids in the complex use of potato mass with wheat germ, soy flour, natural shrimp powder “Rieber Food Ingredients” and lentil-spirulina filling in culinary products have been analyzed. The social effect of fuller use of vegetable, non-fish water raw materials, expansion of the range of vegetable dishes with improved amino acid composition and consumer properties of potato dishes in restaurants, preservation and protection of public health has been confirmed. Conclusions and discussions. Food combinatorics was carried out during the potato croquet technology development with the use of protein vegetable and non-fish water raw materials, which contributed to the improvement of the amino acid composition of vegetable dishes and, in particular, to the increase of their biological value.


Author(s):  
N. Cherno ◽  
K. Naumenko ◽  
L. Gural

Mannans are polysaccharides of natural origin. Their main chain consists of residues of D-mannose. They have an immunomodulatory effect, able to induce macrophage activation, inhibit tumor growth and virus development, normalize blood cholesterol, etc. Manooligosaccharides are effective prebiotics The main way to obtain mannan is to extract their alkaline solutions. This makes it impossible to use this polysaccharide in food technologe. This study proposes a biotechnological method of producing water-soluble mannan from coffee sludge. As a source of mannan used a by-product of obtaining instant coffee at the Odessa Combine Food Concentrates which is formed in the processing of coffee beans Arabica. The chemical composition of this coffee sludge was investigated. It is dominated by water-insoluble carbohydrates which are represented by hemicellulose polysaccharides and cellulose. Analysis of the monosaccharide composition of hemicelluloses showed that the hydrolyzate contains mannose, glucose and galactose residues in a ratio of 6: 0,5: 3., which may indicate the presence of galactomannan in their composition. The developed method involves the treatment of the coffee sludge with enzyme preparation with beta-mannanase activity. The process was carried out in aqueous medium at temperature 50 °C, varying the hydromodule in the range of 30… 50, the ratio of enzyme: substrate (1:25, 1:50 and 1:100) for 24…72 hours. This study presents the characteristics of the monomer composition and molecular weight distribution of polysaccharide samples obtained in this way. Only mannose was found in the hydrolyzate of water-soluble mannan. The rational conditions of enzymatic processing of raw materials are determined. The modified mannan is soluble in water. It contains the highest number of target fragments with a molecular weight of about 20 kDa which are considered to be the most physiologically active. Subsequently the modified mannan can be used in nanotechnology functional food ingredients and dietary supplements.


Author(s):  
L. E. Glagoleva ◽  
N. P. Zatsepilina ◽  
M. V. Kopylov ◽  
S. O. Rodionov

The leading direction in the field of nutrition is the creation of a range of products that contribute to improving health when they are used daily in the diet. The urgency of studying bovine colostrum-colostrum, which is an immune-modeling animal raw material, has been substantiated. Colostrum is a natural source of all the ingredients needed to build immunity. Biologically active substances contained in cow colostrum contribute to: restoration of immunity; restoration of the intestines and stomach; strengthening the nervous system; renewal of brain cells; improving emotional tone and mood; increasing vitality and performance; slowing down the aging process; protection against diseases of the intestines and stomach, cardiovascular system, respiratory tract, diabetes, allergies, osteoporosis and a number of other diseases. Colostrum contains a minimum of 37 immune factors and 8 growth factors that help the body fight disease and promote good health and longevity. The classes of immunoglobulins contained in colostrum, which represent the bulk of whey proteins, have been studied. Colostrum is a limited source of raw materials, its production period is short, there are many opportunities for industrial use, but due to the small amount of raw materials, the market remains undeveloped, with the exception of food additives. Taking into account the extensive therapeutic and medicinal properties of colostrum, its unique composition and properties, its composition and physicochemical properties were investigated, the fractionation parameters were determined using various systems. To process the experimental studies, the STATISTICA 12 software package was used. To obtain the regression equation, the matrix data were processed using the Microsoft Excel 2010 software package. The analysis of the data obtained indicates the possibility and prospects of using colostroma in food production technology.


Author(s):  
С.В. ЗВЕРЕВ ◽  
В.А. ЗУБЦОВ ◽  
В.Г. ЛОБАНОВ ◽  
Ю.Ф. РОСЛЯКОВ ◽  
Е.В. ГЛУХОВА

Исследована комплементарность белка льняного жмыха с белком ряда семян и зерна бобовых культур с целью создания пищевых добавок повышенной биологической ценности для использования в индивидуальном,функциональном, специализированном и спортивном питании. Произведен расчет аминокислотных скоров белка зерна продуктов растениеводства по отношению к эталонному белку ФАО/ВОЗ 2013 г. для человека (без учета усвояемости). По общему уровню белка и основным показателям льняной жмых существенно уступает только соевому жмыху и обрушенному люпину. Однако белок семян льна неполный. Разработан алгоритм оптимизации пятикомпонентных пищевых белковых систем по критерию максимума минимального аминокислотного скора лимитирующих аминокислот смеси. Программа расчета рационального состава белковых смесей выравнивает скоры лимитирующих аминокислот двух компонентов при ограничениях на содержание остальных трех компонентов. Определено, что идеальный белок (18–20%) получается при смешивании льняного жмыха с продуктами переработки зерна амаранта с долей льна (д. л.) 13–27%. Смеси льняного жмыха (ЛЖ) с нутом (Н) и люпином обрушенным (ЛО) с д. л. 37,0 и 6,5% соответственно имеют меньшее общее содержание белка, чем каждая компонента в отдельности, например, %: (ЛЖ + Н) 28, (ЛЖ + ЛО) 39, ЛЖ 31, ЛО 40. Однако смеси содержат большее количество эталонного белка: (ЛЖ + Н) 28%; (ЛЖ + ЛО) 37,6%. Для сравнения, содержание эталонного белка в продукте составляет, %: ЛЖ 23,3; ЛО 36,7; Н 25,0. Установлено, что высокое содержание белка в жмыхе, полученном из семян льна, позволяет создавать смеси с зернобобовыми продуктами, чей белок комплементарен льняному белку, иполучать пищевые системы с белком улучшенного аминокислотного профиля. The complementarity of flaxseed cake protein with the protein of a number of seeds and legume grains was studied in order to create food additives of increased biological value for use in individual, functional, specialized and sports nutrition. The calculation of the amino-acid score of the grain protein of some leguminous crops in relation to the FAO/WHO 2013 reference protein for humans (excluding digestibility) was made. In terms of the overall protein level and main indicators, flaxseed cake is significantly inferior only to soy cake and collapsed lupine. However, the protein of flax seeds is incomplete. An algorithm for optimizing five-component food protein systems based on the criterion of the maximum of the minimum amino-acid score of the limiting amino acids of the mixture is developed. The program for calculating the rational composition of protein mixtures equalizes the scores of limiting amino acids of two components with restrictions on the content of the other three components. It was determined that the ideal protein (18–20%) is obtained by mixing flaxseed cake with products of amaranth grain processing with a proportion of flax of 13–27%. Mixtures of flaxseed cake (FC) with chickpeas (Ch) and collapsed lupine (CL) with a proportion of flax of 37,0 and 6,5%, respectively, have a lower total protein content than each component separately, for example, %: (FC + Ch) 28, (FC + CL) 39, FC 31, CL 40. However, the mixtures contain more of the reference protein: (FC + Ch) 28%; (FC + CL) 37,6%. For comparison, the reference protein content in the product is, %: FC 23,3; CL 36,7; Ch 25,0. It was found that the high protein content in the cake obtained from flax seeds makes it possible to create mixtures with leguminous products whose protein is complementary to flaxseed protein, and to obtain food systems with protein of an improved amino acid profile.


2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Oonagh Smith ◽  
Sarah Kiernan

Abstract Background and Aims Hyperphosphataemia has been independently associated with increased morbidity and mortality across the lifecycle of chronic kidney disease. Dietary phosphate restriction is the corner stone of hyperphosphataemia management. Traditionally the focus was on limiting foods that are naturally high in phosphate such as meats, dairy, wholegrain and nut products. However, phosphate-containing additives are a growing concern. Artificial phosphates are thought to be readily absorbed by the body and are increasingly being added to processed foods, at a time when there is an increased desire and reliance on convenience foods. It has been shown that educating patients with end stage renal disease to avoid phosphate-containing food additives can result in a modest but clinically significant improvement in serum phosphorus levels. 1 The aim of this study was to evaluate how feasible it is for a patient to source convenience packaged cooked meats/poultry that are free from artificial phosphate and if such products are a suitable option for the renal patient in relation to salt and protein content. Method Data was manually collected in a branch of the five leading supermarket chains. The ingredient list of each product food label was checked for artificial phosphate (phos), artificial potassium (K) or the corresponding E numbers and the nutrition table examined for salt and protein content (per 100g / 25g portion or slice). The price was checked in store and manually calculated per 7g protein exchange. Results


2020 ◽  
Vol 11 (1) ◽  
pp. 908-912 ◽  
Author(s):  
Olga A. Gracheva ◽  
Alizade S. Gasanov ◽  
Damir R. Amirov ◽  
Bulat F. Tamimdarov ◽  
Dina M. Mukhutdinova ◽  
...  

Amino acids are the basic structural units of protein molecules in the body. Currently, about 300 amino acids are known, of which 26 are studied best. Amino acids or their derivatives (for example, immune bodies) are part of enzymes, hormones, pigments, and other specific substances that play a crucial role in digestive and metabolic processes. In the process of metabolism, many amino acids are synthesized in the body from other amino acids or compounds, and therefore they are called interchangeable. Amino acids that are not synthesized in the body or are formed in insufficient quantities are called indispensable. According to the content and ratio of essential amino acids, feed proteins are subdivided into full and inferior. Deficiency, absence, or imbalance of essential amino acids in animal diets is accompanied by a deterioration in protein use, metabolic disorders, and decreased productivity. The following amino acids are indispensable; arginine, viburnum, histidine, isoleucine, leucine, lysine, methionine, threonine, tryptophan, phenylalanine. Among the essential amino acids, especially important ones, are those called critical. These are lysine, methionine + cystia, threonine, and tryptophan. A deficiency, absence, or imbalance of essential amino acids in animal diets is accompanied by a deterioration in protein use, metabolic disorders, and decreased productivity. Research and production experiment was conducted within Olenka Poultry Factory LLC and the problematic research laboratory of feed additives of the Department of Animal Feeding and Feed Technology named after P. D. Pshenichny at the National University of Life and Environmental Sciences. The effective level of arginine in compound feed for broilers of the Cobb-500 cross was experimentally determined. The use of compound feed with arginine levels in the first rearing period (1 to 10 days) - 1.28%, in the second (11 to 22 days) - 1.15% and in the third (23 to 42 days) - 1.11% gives the opportunity to get broiler chickens at the age of 42 days, weighing 2.654 kg, at a feed expenditure of 1.78 kg per 1 kg of gain.


2020 ◽  
Vol 200 ◽  
pp. 210-220
Author(s):  
O. Ya. Mezenova ◽  
L. S. Baydalinova ◽  
N. Yu. Mezenova ◽  
S. V. Agafonova ◽  
E. A. Kazimirova ◽  
...  

Processing of secondary raw materials from production of canned food «Sprats in oil» is considered. Comprehensive technology is developed for producing of protein, fat, and protein-mineral food additives from the sprat waste, as smoked sprat heads, using the method of high temperature hydrolysis. Rational method of the hydrolysis is substantiated, including preliminary separation of fat in fatty raw materials and its enzymatic-thermal treatment. The optimal values of temperature and duration of hydrolysis in autoclave are determined. Balances of organic substances are calculated for the main operations. This new technology was tested for fatty (24 %) and medium fat (13 %) raw materials under the temperature of 130–160 о С. The hydrolyzed organic mass was divided into fat, protein, and mineralized fractions and proteinfat emulsion. Food additives containing low molecular weight water-soluble peptides and high molecular weight insoluble proteins were extracted from the protein fraction by freeze-drying and mineral compounds were extracted from the mineral fraction by convective drying. The proteins were extracted more thoroughly, so the content of low molecular weight peptides in the freeze-dried hydrolysate of protein fraction was > 80 %. The produced additives have pleasant organoleptic properties and are sanitary safe. They are tested with some seafood, with positive result. The developed technology for processing of smoked waste is economically valuable and allows to solve the problem of pollution in the fish smoking industry.


Food Industry ◽  
2020 ◽  
Vol 5 (3) ◽  
pp. 44-51
Author(s):  
Olga Mitryashkina ◽  
Lydia Shulgina ◽  
Yuriy Shulgin

To activate the elastic tissues regeneration, a man needs to receive at least 5 g of such amino acids as proline and oxyproline, which are involved in the process of the body’s own collagen production, with food every day. The source of collagen can be products made from the hearts of farm animals. High temperature influence destroys collagen, and there is an accumulation of low-molecular peptides that are available to the human body. The article presents the results on the development of new types of canned food based on the farm animal hearts and plant raw materials. The animal hearts are high-protein raw materials in terms of protein content. The fat content does not exceed 3.7 ± 0.5 %, which results in a lower energy value compared to muscle tissue. Proteins in animal hearts are balanced in terms of amino acid composition; the content of collagen in them is 4.8–4.5 %, which is significantly higher than its amount in muscle tissue proteins. The researchers developed technology and recipes of canned food based on animal hearts and vegetable raw materials. The mass fraction of hearts in canned food recipes is 60.0–65.0 %. The additional components were beans and vegetables. Developed canned food made on the basis of animal hearts and vegetable raw materials had high organoleptic characteristics: protein content was 10.7–12.6 %; fat – 7.6–9.1 %. 100 g of canned food contains 0.85 ± ± 0.5 g of collagen-forming amino acids, which allows the human body to provide 16.5 ± ± 0.5 % of the daily need for them. Canned food is an additional source of iron (the content of which is 2.9 ± 0.3 g/100 g). A man recommends canned foods based on animal hearts and vegetable raw materials as specialized products for dietary and preventive nutrition of people of older age groups and persons with reduced processes of elastic tissue regeneration.


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