scholarly journals In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum albumin nanoparticles: in vitro cytocompatibility studies

2017 ◽  
Vol Volume 12 ◽  
pp. 4073-4084 ◽  
Author(s):  
Priyanka Singh ◽  
Yeon Ju Kim ◽  
Hina Singh ◽  
Sungeun Ahn ◽  
Verónica Castro-Aceituno ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Water Soluble ◽  
Ginsenoside Rh2 ◽  
Albumin Nanoparticles ◽  
In Situ Preparation

Bovine Serum Albumin Nanoparticles Containing Amphotericin B: Characterization, Cytotoxicity and In Vitro Antifungal Evaluation

2015 ◽  
Vol 15 (12) ◽  
pp. 10183-10188 ◽  
Author(s):  
Diani Meza Casa ◽  
Thaysa Ksiaskiewcz Karam ◽  
Aline de Cristo Soares Alves ◽  
Aline Aparecida Zgoda ◽  
Najeh Maissar Khalil ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Amphotericin B ◽  
Bovine Serum ◽  
Albumin Nanoparticles

Formulation, Characterization and In-Vitro Evaluation of 6-Azauridine Loaded Bovine Serum Albumin Nanoparticles

2014 ◽  
Vol 2 (4) ◽  
pp. 288-293
Author(s):  
Sunanda Dhavala ◽  
B. S. Harish ◽  
G. Poorani ◽  
Veerappan Anbazhagan ◽  
Kiran Babu Uppuluri
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Albumin Nanoparticles

Vascular perfusion: a novel means of studying oviduct function

1985 ◽  
Vol 248 (5) ◽  
pp. E624-E632 ◽  
Author(s):  
H. J. Leese ◽  
S. M. Gray
Keyword(s):  
Amino Acids ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Vascular Perfusion ◽  
Ovarian Artery ◽  
In Situ Preparation ◽  
Luminal Perfusion ◽  
Oviduct Fluid

An in situ preparation for the combined vascular and luminal perfusion of the rabbit oviduct has been developed. Medium 199, gassed with 5% CO2 in O2 and supplemented with heparin, antibiotics, and 2.5% wt/vol dialyzed bovine serum albumin was infused into the ovarian artery at a rate of 1 ml/min. Krebs Ringer bicarbonate medium was recirculated through the lumen at a rate of 50 microliter/min. The ovary was perfused together with the oviduct, and the preparation is viable for up to 3 h. Equal concentrations of pyruvate, lactate, glucose, and sucrose added to the vascular medium were transported at different rates into the lumen, as was a physiological mixture of amino acids. A proportion of the lactate entering the lumen was synthesized within the oviduct from vascular glucose. When glucose and pyruvate were omitted from the vascular medium, their appearance and that of lactate in the lumen was barely detectable, suggesting that these oviduct fluid components are mainly derived from the blood. The oviduct maintained a steady transmural potential difference of 5.9 mV (lumen negative). With vascular perfusion alone, oviduct fluid entered the oviduct lumen at a rate of 16.8 microliter/h. In oviducts taken from rabbits 3 days postovulation, there was a general decrease in the vascular to lumen flux of all nutrients measured. Preliminary work has shown that the preparation may be used to study ovulation, ovum pickup and transport, and fertilization.

scholarly journals PEGylation of Ginsenoside Rg3-Entrapped Bovine Serum Albumin Nanoparticles: Preparation, Characterization, and In Vitro Biological Studies

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Lijun Zhang ◽  
Junfeng Hui ◽  
Pei Ma ◽  
Yu Mi ◽  
Daidi Fan ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Drug Loading ◽  
Entrapment Efficiency ◽  
Ginsenoside Rg3 ◽  
Albumin Nanoparticles ◽  
Drug Entrapment Efficiency ◽  
Drug Loading Efficiency

Ginsenoside Rg3 (Rg3) is one of three triterpene saponins from red ginseng. It has important structural functions and pharmacological properties. However, due to its poor solubility, low bioavailability, and short half-life in blood circulation, its clinical application was unsuccessful for the treatment of a variety of cancers. In order to overcome this limitation, this study prepared mPEGylation-Rg3 bovine serum albumin nanoparticles (mPEG-Rg3-BSA NPs). The characteristics of the NPs, such as drug entrapment efficiency, drug loading efficiency, surface morphology, thermal stability, and cytotoxicity in vitro, were investigated. The results showed that the appropriate particle size of the obtained NPs was 149.5 nm, the water solubility and stability were better than free Rg3, and the drug entrapment efficiency and drug loading efficiency were 76.56% and 17.65%, respectively. Moreover, the cytotoxicity assays of the mPEG-Rg3-BSA NPs and free Rg3 revealed that the mPEG-Rg3-BSA NPs have greater anticancer effects in HepG2 cells and A549 cells. However, the cytotoxic effect of free Rg3 was higher than the mPEG-Rg3-BSA NPs in L929 cells. The results indicated that using the mPEGylation method and selecting BSA as a carrier to form the nanodrug carrier system were effective for improving the properties of Rg3.

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