scholarly journals A COMPARATIVE CLINICAL EVALUATION FOR PRECISE ISOLATION USING ADDITION SILICONE DAM COVERAGE WITH CONVENTIONAL ISOLATION TECHNIQUES- AN IN-VIVO STUDY

2021 ◽  
Vol 9 (11) ◽  
pp. 111-115
Author(s):  
Shradha Suman ◽  
◽  
Prakash Lokhande ◽  
Dhanyakumar N.M ◽  
Siddheswaran V. ◽  
...  

Context: A proper isolation technique plays a key role in the success of restoration of carious tooth. Aim Of The Study: The aim of this in-vivo study was to evaluate efficacy of addition silicone dam coverage technique with conventional isolation methods in restorative procedures and endodontic treatment. Settings And Design: Forty-Five patients who fulfill the inclusion criteria were selected for the study. Materials And Methods: All the participants underwent clinical procedures using 3 different isolation techniques. Group-1: Clinical procedures were carried out using traditional cotton roll isolation. Group-2: Clinical procedures were carried out using conventional rubber dam isolation procedure. Group-3: Clinical procedures were carried out using addition silicone dam coverage technique. The efficacy of isolation in terms of clinician usage and patient comfort is evaluated by a single evaluator. The results were tabulated and statistically analyzed. Statistical Analysis: IBM SPSS (version 21.0) software was used. Chi-square test was performed, considering P < 0.05 for statistical significance. Results: All the groups demonstrated satisfactory clinical performance. Upon inter and intra-group comparison of the isolation methods, there was statistically significant difference (P > 0.05). Conclusion: It is important to achieve an aseptic environment in clinical restorative and endodontic procedures. Addition silicone dam coverage technique is a chair side modified design which is user friendly with improved treatment efficacy. However, long-term clinical studies must be needed for further evaluation.

2008 ◽  
Vol 74 (7) ◽  
pp. 1997-2003 ◽  
Author(s):  
Mathieu Millette ◽  
Gilbert Cornut ◽  
Claude Dupont ◽  
François Shareck ◽  
Denis Archambault ◽  
...  

ABSTRACT This study demonstrated the capacity of bacteriocin-producing lactic acid bacteria (LAB) to reduce intestinal colonization by vancomycin-resistant enterococci (VRE) in a mouse model. Lactococcus lactis MM19 and Pediococcus acidilactici MM33 are bacteriocin producers isolated from human feces. The bacteriocin secreted by P. acidilactici is identical to pediocin PA-1/AcH, while PCR analysis demonstrated that L. lactis harbors the nisin Z gene. LAB were acid and bile tolerant when assayed under simulated gastrointestinal conditions. A well diffusion assay using supernatants from LAB demonstrated strong activity against a clinical isolate of VRE. A first in vivo study was done using C57BL/6 mice that received daily intragastric doses of L. lactis MM19, P. acidilactici MM33, P. acidilactici MM33A (a pediocin mutant that had lost its ability to produce pediocin), or phosphate-buffered saline (PBS) for 18 days. This study showed that L. lactis and P. acidilactici MM33A increased the concentrations of total LAB and anaerobes while P. acidilactici MM33 decreased the Enterobacteriaceae populations. A second in vivo study was done using VRE-colonized mice that received the same inocula as those in the previous study for 16 days. In L. lactis-fed mice, fecal VRE levels 1.73 and 2.50 log10 CFU/g lower than those in the PBS group were observed at 1 and 3 days postinfection. In the P. acidilactici MM33-fed mice, no reduction was observed at 1 day postinfection but a reduction of 1.85 log10 CFU/g was measured at 3 days postinfection. Levels of VRE in both groups of mice treated with bacteriocin-producing LAB were undetectable at 6 days postinfection. No significant difference in mice fed the pediocin-negative strain compared to the control group was observed. This is the first demonstration that human L. lactis and P. acidilactici nisin- and pediocin-producing strains can reduce VRE intestinal colonization.


2017 ◽  
Vol 38 (6) ◽  
pp. 3647
Author(s):  
Claudia Lizandra Ricci ◽  
Rogério Giuffrida ◽  
Glaucia Prada Kanashiro ◽  
Hilidia Stephania Rufino Belezzi ◽  
Carolina De Carvalho Bacarin ◽  
...  

The objective of this study was to evaluate the use of the Kowa HA-2 applanation tonometer in measuring intraocular pressure (IOP) in cats. Ten healthy eyes were used in an ex vivo study in which the calibration curve for manometry vs. tonometry was determined by artificially raising the IOP in 5 mmHg increments up to 60 mmHg (10-60 mmHg). Both eyes of 10 anesthetized cats were studiedin vivo to compare manometry vs. tonometry. In the ambulatory study, 78 healthy eyes, 7 eyes with glaucoma and 20 eyes with uveitis were evaluated by tonometry, which was performed with topical anesthesia and 1% fluorescein eye drops for the formation of fluorescein semicircles. The correlation coefficient (r²) between the manometer and the Kowa HA-2 tonometer was 0.993 and the linear regression equation was y = 0.0915x + 0.0878 in the ex-vivo study. In the in vivo study, the IOP values (mean±SD, in mmHg) in manometry were 15.6 ± 1.1(14.0 – 17.5) and in tonometry were 15.5 ± 1.2(13.5 – 17.2), with no significant difference (P > 0.05) between manometry and tonometry. In ambulatory study, using the Kowa HA-2 tonometer, the IOP values (mean±SD, in mmHg) were 15.0 ± 1.5 (11.8 – 18.3) for the healthy eyes, 38.4 ± 8.1(29.6 – 53.7) for glaucomatous eyes and 10.4 ± 2.0(5.3 – 12.2) for eyes with uveitis. There was a strong correlation and accuracy between the IOP values with the manometry and the Kowa HA-2 tonometer. In the ambulatorystudy the IOP values obtained with the tonometer were compatible for animals with healthy eyes and with clinical signs of glaucoma and uveitis. We conclude that the Kowa HA-2 tonometer can be used in the measurement of IOP in cats, since it is a practical and accurate method in this species.


Author(s):  
Anil K. Tomer ◽  
Hysum Mushtaq ◽  
Anila Krishna Saxena ◽  
Megna Bhatt ◽  
Ayush Tyagi ◽  
...  

Objectives: The aim of this study was to evaluate the clinical performance of a nano filled flowable and nano hybrid bulk fill resin composite in class I restorations. Methods and Materials: Twenty patients were selected for this in vivo study. Each patient received at least one pair of restorations, restored with nano hybrid bulk fill resin composite (IPS Empress direct [IED]) and nano hybrid Tetric N Ceram flowable composite [TNC]. Each restorative resin system was used with its respective adhesive system according to manufacturers’ instructions. A total of 40 class I restorations were placed by one operator.  Restorations were blindly evaluated by two examiners at baseline and 3, 6, and 12 months respectively using modified US Public Health Service Ryge criteria. The data obtained was statistically analyzed using Chi square test to compare the two restorative materials for each category. Results: At 3, 6 and 12, months, recall rate was 100%, 95% and 85%, respectively, with a retention rate of 100%. There were statistically significant differences between the two restorative resins in terms of marginal adaptation and marginal discoloration (p<0.05). No differences were observed between the restorative resins in terms of retention (p<0.05). None of the restorations showed postoperative sensitivity, or loss of anatomic form. Conclusion: Within the limitations of this study, nano hybrid bulk fill composite resin viz. IPS EMPRESS DIRECT showed better clinical performance than nano filled flowable composite in terms of marginal discoloration and marginal adaptation. Keywords: direct composite, bulk, hybrid filled resin


2015 ◽  
Vol 5 (2) ◽  
pp. 74 ◽  
Author(s):  
PriyankaL Kalimireddy ◽  
AR Prabhakar ◽  
Chandrashekar Yavagal ◽  
S Sugandhan

2003 ◽  
Vol 10 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Brigitte Vollmar ◽  
Jan Slotta ◽  
Ruth Nickels ◽  
Ernst Wenzel ◽  
Michael Menger

2015 ◽  
Vol 44 (1) ◽  
pp. 24-30
Author(s):  
Patrícia Lins Azevedo do Nascimento ◽  
Rafael Bezerra Ribeiro ◽  
Cícero Romão Gadê-Neto ◽  
Alexandre Henrique de Moura Dias

AIM: To assess dimensional change and antimicrobial activity of disinfectants substances incorporated during the dental stone manipulation. MATERIAL AND METHOD: In vivo - microorganisms were collected in alginate molds of 30 volunteers inoculated on BHI agar and incubated at 37 °C for 24 hours. The molds were cast with type IV gypsum, manipulated with saline (G1), 1% sodium hypochlorite (G2) and 4% chlorhexidine (G3), replacing the water. After setting of plaster with 1 hour two collections on models were made. After 24 hours, the readings were performed. The Kruskal-Wallis and Wilcoxon tests with confidence interval of 99% and 95% respectively were used. In vitro - Müeller Hinton agar petri dishes were inoculated with S. mutans (ATCC25175), S. sanguis (ATCC10556) and E. faecalis (ATCC29212), over which were placed steel rings filled with the same substances of the in vivo study. After deposition of gypsum and incubation, halos were measured with a digital caliper and data were submitted to ANOVA and Tukey's test with confidence interval of 95%. Dimensional Change - With a metallic matrix and a perfectly adapted tray, the insertion axis and force used for moulding and obtain 30 specimens in type IV gypsum were standardized, following the same distribution of the study groups in vivo. The specimens were measured by Image Pro Plus software and data were submitted to ANOVA and Tukey's test with confidence interval of 95%. RESULT: Data from the in vivo study demonstrated a significant difference between the mold and each model (p<0.001). In the Wilcoxon test there was no significant difference between groups of models. At the in vitro test, G2 showed greater inhibition zones in all micro-organisms tested compared to G3, but with respect to dimensional changes, there was a significant difference between solutions and metallic standard, where G3 caused less change than G2. CONCLUSION: Chlorhexidine 4% showed to be the most suitable disinfectant.


2017 ◽  
Vol 87 (6) ◽  
pp. 841-846 ◽  
Author(s):  
Aslihan Zeynep Oz ◽  
Abdullah Alper Oz ◽  
Sabahat Yazıcıoglu

ABSTRACT Objectives: The aim of this in vivo study was to investigate the preventive effect of two different adhesives on enamel demineralization and compare these adhesives with a conventional one. Materials and Methods: Fifteen patients requiring the extraction of their first four premolars for orthodontic treatment were included in the study. One premolar was randomly selected, and an antibacterial monomer-containing and fluoride-releasing adhesive (Clearfil Protect Bond, Kuraray Medical, Okayama, Japan) was used for orthodontic bracket bonding. Another premolar was randomly selected, and a fluoride-releasing and recharging orthodontic adhesive (Opal Seal, Ultradent Products, South Jordan, Utah) was used. One premolar was assigned as a control, and a conventional adhesive (Transbond XT, 3M Unitek, Monrovia, Calif) was used. The teeth were extracted after 8 weeks, and the demineralization areas of the 45 extracted teeth were analyzed using microcomputed tomography with software. Results: There was no significant difference between the white spot lesion (WSL) rates of the adhesives (P &gt; .05). The volumes of the WSLs varied from 0 to 0.019349 mm3. Although Opal Seal showed the smallest lesion volumes, there was no significant difference in volumetric measurements of the lesions among the groups (P &gt; .05). Conclusions: The findings indicated no significant differences between the preventive effects of the adhesives used in this in vivo study over 8 weeks.


2016 ◽  
Vol 138 (12) ◽  
Author(s):  
Jung Joo Kim ◽  
David S. Musson ◽  
Brya G. Matthews ◽  
Jillian Cornish ◽  
Iain A. Anderson ◽  
...  

We have developed a novel cell stretching device (called Cell Gym) capable of applying physiologically relevant low magnitude strains to tenocytes on a collagen type I coated membrane. We validated our device thoroughly on two levels: (1) substrate strains, (2) cell level strains. Our cell level strain results showed that the applied stretches were transferred to cells accurately (∼90%). Our gene expression data showed that mechanically stimulated tenocytes (4%) expressed a lower level of COL I gene. COX2 gene was increased but did not reach statistical significance. Our device was then tested to see if it could reproduce results from an in vivo study that measured time-dependent changes in collagen synthesis. Our results showed that collagen synthesis peaked at 24 hrs after exercise and then decreased, which matched the results from the in vivo study. Our study demonstrated that it is important to incorporate physiologically relevant low strain magnitudes in in vitro cell mechanical studies and the need to validate the device thoroughly to operate the device at small strains. This device will be used in designing novel tendon tissue engineering scaffolds in the future.


2021 ◽  
Vol 12 (1) ◽  
pp. 144-152
Author(s):  
Minal Gopal Tulsani ◽  
Dhanraj Ganapathy ◽  
Divya Rupawat ◽  
Sanjana Devi

Aim: To evaluate the effectiveness of midazolam and zolpidem on postoperative pain perception in patients undergoing implant placement. Materials and Methods: In the present in vivo study 60 patients undergoing implant placement were selected based on the inclusion criteria framed and were randomly allocated using sequentially numbered, opaque, and sealed envelope (SNOSE) method into 3 groups with 20 patients each after obtaining informed consent. Group A was the control group, Group B received midazolam 7.5 mg 30 minutes before the procedure. Group C received zolpidem 5 mg 30 minutes before the procedure. The anxiety level of patients was recorded using the Corah scale and postoperative pain was recorded after 2 hours of implant placement using the VAS scale. Statistical analysis was done using analysis of variance (ANOVA), one-way multivariate analysis of variance (one-way MANOVA), and then Tukey’s Honestly Significant Difference (HSD) test for comparison among groups at the 0.05 level of significance. Results: Group A had a mean anxiety level of 16 ± 1.451, Group B had a mean anxiety level of 11.2 ± 2.858, and Group C had a mean anxiety level of 13 ± 2.9019 and a statistically significant difference between the groups was observed ( P < .05). The mean for the postoperative pain perception for Group A was 6.8 ± 1.1965, for Group B was 3.8 ± 1.3611, and Group C was 5 ± 1.451 and a statistically significant difference between the groups was observed ( P < .05). Conclusion: This study concluded that both midazolam and zolpidem significantly reduced anxiety levels and postoperative pain in patients undergoing implant placement.


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