scholarly journals IOT BASED MOLECULAR MARKERS FOR DISEASE RESISTANCE STUDIES IN RICE - STRATEGY AND CHALLENGES

2021 ◽  
Vol 9 (11) ◽  
pp. 319-322
Author(s):  
Yeswanth Sai Kumar Thota ◽  
◽  
Harshitha Uriti ◽  
Sree Lakshmi Mandadapu ◽  
Shaik Mohammed Junaid ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Magnaporthe Grisea ◽  
Brown Planthopper ◽  
High Yield ◽  
Rice Yellow Mottle Virus ◽  
Viral Origin ◽  
Length Polymorphisms ◽  
Yellow Mottle ◽  
The Internet Of Things ◽  
Simple Sequence

Rice production is constrained by diseases of fungal, bacterial and viral origin. The Internet of Things (IoT) – network of interconnected devices - is an application for disease related uses, collection of data, processing for testing and monitoring. This review article aims about how IoT can track and allows disease resistance studies in in Oryza species. Among them Xanthomonas oryzae, Magnaporthe grisea, RYMV (Rice yellow mottle virus), and brown planthopper causes the high yield losses. Disease resistance genes are identified and they are screened by the SSR (simple sequence repeats), RAPD (Randomly Amplified Polymorphic DNA) and RFLP (restriction fragment length polymorphisms) analysis.

scholarly journals Relative resistance to Rice yellow mottle virus in rice 

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 1-8 ◽  
Author(s):  
M.T. Salaudeen
Keyword(s):  
Agronomic Traits ◽  
Disease Incidence ◽  
Mottle Virus ◽  
High Yield ◽  
Rice Yellow Mottle Virus ◽  
Rice Cultivars ◽  
Progress Curve ◽  
Breeding Programmes ◽  
Yellow Mottle ◽  
Paddy Yield

We identified sources of Rice yellow mottle virus (RYMV) resistance in rice cultivars. Eight cultivars together with susceptible and resistant controls were evaluated under screenhouse conditions as inoculated and uninoculated treatment in completely randomised design with three replications. Seedlings were inoculated with the virus by sap transmission at two weeks after sowing. Disease incidence and severity (scale 1–9: 1–3 = green leaves with sparse dots or streaks, 9 = yellow or orange leaves and some plant dead), yield, and agronomic traits were recorded. Data analyses included Area Under the Disease Progress Curve (AUDPC), independent t-test, and Analysis of Variance. According to differences in most measured traits control cultivars FARO 29 and Gigante were proved to be the most susceptible and partially tolerant ones, respectively. Cvs FARO 12, FARO 17, FARO 37, and FARO 52 were classified as partially tolerant. Uninoculated control plants performed better than the inoculated for all the yield and agronomic parameters. Reduction in plant height (6%) and number of tillers per plant (4.8%), increased days to heading (3 days), and reduction in paddy yield (6.5%) was lowest in cv. Gigante. Paddy yield per plant of the RYMV-inoculated was the highest in cv. Gigante (2.4 g). The rice cultivars which combined RYMV-resistance with high-yield could be utilised in rice breeding programmes in order to enhance food security.

scholarly journals The rice yellow mottle virus P1 protein exhibits dual functions to suppress and activate gene silencing

2010 ◽  
Vol 61 (3) ◽  
pp. 371-382 ◽  
Author(s):  
Séverine Lacombe ◽  
Martine Bangratz ◽  
Florence Vignols ◽  
Christophe Brugidou
Keyword(s):  
Gene Silencing ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Yellow Mottle ◽  
Activate Gene ◽  
Dual Functions

OAC Elmira winter barley

1997 ◽  
Vol 77 (4) ◽  
pp. 639-640 ◽  
Author(s):  
D. E. Falk ◽  
E. Reinbergs ◽  
G. Meatherall
Keyword(s):  
Disease Resistance ◽  
Hordeum Vulgare ◽  
Winter Hardiness ◽  
Winter Barley ◽  
High Yield ◽  
Science Department ◽  
Hordeum Vulgare L ◽  
Southern Ontario ◽  
Agriculture And Food ◽  
The University

OAC Elmira is a high-yielding, disease-resistant, hardy winter barley adapted to Southern Ontario. OAC Elmira has good winter hardiness and high hectoliter weight. It has better disease resistance than any of the check cultivars and long straw with a lax, nodding head. It was developed by the Ontario Ministry of Agriculture and Food through the Crop Science Department of the University of Guelph. Key words: Hordeum vulgare L., high yield, disease resistance, winter hardiness

Molecular epidemiology of the RNA satellite of Rice yellow mottle virus in Africa

2003 ◽  
Vol 148 (9) ◽  
pp. 1721-1733 ◽  
Author(s):  
A. Pinel ◽  
Z. Abubakar ◽  
O. Traor� ◽  
G. Konat� ◽  
D. Fargette
Keyword(s):  
Molecular Epidemiology ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Yellow Mottle

scholarly journals Use of simple sequence repeat (SSR) markers for screening blue disease resistance in cotton germplasm exchange

2015 ◽  
Vol 14 (41) ◽  
pp. 2871-2875 ◽  
Author(s):  
Faustine Christopher ◽  
Vieira Hoffmann Lucia ◽  
Ismail Tibazarwa Flora ◽  
Lukonge Everina
Keyword(s):  
Disease Resistance ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Germplasm Exchange ◽  
Simple Sequence

scholarly journals Synthesis of an infectious full-length cDNA clone of rice yellow mottle virus andmutagenesis of the coat protein

Virology ◽  
1995 ◽  
Vol 206 (1) ◽  
pp. 108-115 ◽  
Author(s):  
C. Brugidou ◽  
C. Holt ◽  
M. Ngon A Yassi ◽  
S. Zhang ◽  
R. Beachy ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cdna Clone ◽  
Full Length ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Full Length Cdna ◽  
Yellow Mottle

scholarly journals The Genetic Basis of High Resistance to Rice Yellow Mottle Virus (RYMV) in Cultivars of Two Cultivated Rice Species

Plant Disease ◽  
1999 ◽  
Vol 83 (10) ◽  
pp. 931-935 ◽  
Author(s):  
M. N. Ndjiondjop ◽  
L. Albar ◽  
D. Fargette ◽  
C. Fauquet ◽  
A. Ghesquière
Keyword(s):  
Genetic Basis ◽  
Virus Isolate ◽  
Mottle Virus ◽  
F1 Hybrids ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rice Yellow Mottle Virus ◽  
Recessive Resistance ◽  
Cultivated Rice ◽  
Recessive Resistance Gene ◽  
Yellow Mottle

Three cultivars of Oryza sativa (IR64, Azucena, and Gigante) and four cultivars of O. glaberrima (Tog5681, Tog5673, CG14, and SG329) were evaluated for their resistance to two isolates of rice yellow mottle virus (RYMV) by enzyme-linked immunosorbent assay (ELISA) and symptomatology. Cultivars Tog5681 and Gigante were highly resistant, and no symptoms were observed when either virus isolate was inoculated at 10 or 20 days postgermination and assayed by ELISA at 7, 14, 22, 35, 50, or 64 days postinoculation. Azucena showed a partial resistance, whereas the other cultivars were susceptible. Symptom appearance was associated with increase in ELISA absorbance in the systemically infected leaves. The best discrimination among the cultivars occurred when the plants were inoculated at 10 days postgermination. Crosses were made between the highly resistant (Gigante and Tog5681) and the susceptible (IR64) cultivars to determine the genetic basis of resistance to RYMV. Evaluation of F1 hybrids and interspecific progenies, as well as the segregation of resistance in F2 and F3 lines of the IR64 × Gigante cross, provided results consistent with the presence of a single recessive resistance gene common to Tog5681 and Gigante.

scholarly journals Seleksi Berbasis Marka Molekuler pada Padi Generasi F2 Guna Merakit Galur Padi Harapan Tahan Wereng Coklat

Agrikultura ◽  
2016 ◽  
Vol 27 (1) ◽  
Author(s):  
Nono Carsono ◽  
Gigih Ibnu Prayoga ◽  
Neni Rostini ◽  
Danar Dono
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Yield Loss ◽  
Insect Pest ◽  
Brown Planthopper ◽  
F2 Population ◽  
Significant Yield ◽  
F2 Generation ◽  
F2 Progeny ◽  
Simple Sequence

ABSTRACTMolecular Marker-based Selection on F2 Progeny for Development of Promising Rice Lines Resistant to Brown PlanthopperBrown planthopper (BPH) is the major insect pest of rice and accounts for significant yield loss. This experiment was aimed to develop BC1F1 and F3 brown planthopper resistant rice lines. Selection on the basis of SSR markers can be done by using two polymorphic SSR markers, i.e., RM586 dan RM8213, which screened from eight SSR markers for BPH resistant. Sixty-three F2 genotypes from IP-1 (derived from IR-64 x PTB-33) population and twenty F2 genotypes from PP-11 (derived from Pandan Wangi x PTB-33) population were selected and will be used for further research by selfed and backcrossed to recipient parents. Chi-squares test for segregation of DNA bands in F2 generation showed that RM8213 fitted with 1:2:1 Mendelian ratio for controlling photosynthetic rates and trichomes length in IP-1 population. This information could be used in programs to develop a durable brown planthopper resistant rice cultivar.Keywords: BPH, F2 population, Moleculer marker, SSRABSTRAKWereng coklat merupakan salah satu hama utama pada tanaman padi yang mampu menurunkan produksi padi secara nyata. Penelitian ini bertujuan untuk memperoleh galur-galur padi F2 yang memiliki marka-marka yang berasosisasi dengan ketahanannya terhadap wereng coklat. Seleksi pada galur padi F2 hasil persilangan telah dilakukan melalui teknik marka molekuler Simple Sequence Repeat (SSR) menggunakan dua marka SSR yang menunjukkan polimorphisme yaitu RM586 dan RM8213 dari delapan marka yang diskrining. Sebanyak 63 genotip dari populasi IP-1 (hasil persilangan IR-64 x PTB-33) dan 20 genotip dari populasi PP-11 (hasil persilangan Pandan Wangi x PTB-33) untuk disilangkan sendiri maupun disilang balik dengan tetua recipient. Selain itu, hasil analisis Chi-Kuadrat untuk segregasi pita DNA menunjukkan bahwa primer RM8213 memiliki rasio 1:2:1 (dominasi tidak sempurna) dalam mengontrol karakter laju fotosintesis dan panjang trikoma terhadap wereng coklat pada populasi IP-1. Informasi yang diperoleh dari penelitian ini nantinya dapat digunakan untuk program perakitan kultivar padi tahan wereng coklat yang durable.Kata Kunci: Marka molekuler, Populasi F2, SSR, Wereng coklat

scholarly journals Pengujian Kemurnian Genetik Benih Padi Galur F3 (Pandanwangi x PTB33) Terseleksi Menggunakan Marka Molekuler Simple Sequence Repeats (SSR)

Agrikultura ◽  
2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Syindy Raffini Nasihin ◽  
Wieny H. Rizky ◽  
Nono Carsono
Keyword(s):  
Simple Sequence Repeats ◽  
Simple Sequence Repeat ◽  
Brown Planthopper ◽  
Plant Analysis ◽  
Current Experiment ◽  
Genetic Purity ◽  
Experimental Station ◽  
Purity Testing ◽  
Brown Planthopper Resistance ◽  
Simple Sequence

ABSTRACTSeed Purity Testing of F3 Progeny of Rice Lines Derived from a Cross between Pandanwangi x PTB-33 Estimated by Simple Sequence Repeat MarkersSeeds with high purity is required to produce maximum crop yield. Genetic purity of selected F3rice seed progenies derived from a cross between Pandanwangi x PTB33 was estimated by SSR(simple sequence repeats) molecular markers. Objective of current experiment was to obtain riceseed with high genetic purity (100%) in terms of homogeneity of alleles. The experiment wasconducted at Laboratory of Plant Analysis and Biotechnology, meanwhile field experiment wasperformed at Ciparanje Experimental Station, Faculty of Agriculture, Universitas Padjadjaran.Based on primer designed by Bradbury (aromatic trait) and primers RM589 and RM586 (supposedto correlate with brown planthopper resistance gene), seeds of F3 selected had 100% geneticpurity. SSR markers applied for each offspring were able to demonstrate the purity of the seed.Genotypes with 100% genetic purity can be continued for propagating their seeds.Keywords: F3, seed purity, seed rice, SSR markersABSTRAKBenih dengan kemurnian genetik tinggi sangat diperlukan untuk produksi tanaman yangmaksimal. Kemurnian genetik padi generasi F3 hasil persilangan Pandanwangi x PTB-33 diestimasidengan menggunakan marka molekuler SSR. Percobaan ini bertujuan untuk mendapatkan generasiF3 yang memiliki kemurnian genetik 100%. Penelitian dilaksanakan di Kebun PercobaanCiparanje, Fakultas Pertanian Universitas Padjadjaran dan Laboratorium Analisis dan BioteknologiTanaman. Hasil analisis menggunakan primer Bradbury menunjukkan 100% benih murniberdasarkan karakter aromatik, begitupun berdasarkan karakter ketahanan terhadap wereng(primer RM589 dan RM586) menunjukkan 100% benih murni. Marka molekuler SSR yangdigunakan untuk verifikasi kemurnian mampu menunjukkan kemurnian genetik benih padi yangtinggi. Genotip PP dapat dilanjutkan untuk pengujian dan atau perbanyakan benih sumber.Kata kunci: benih padi, F3, marka SSR, uji kemurnian genetik

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