scholarly journals ARTIFICIAL FIELD INOCULATION OFBREAD WHEAT ADVANCED LINES WITH TILLETIAINDICA, CAUSAL AGENT OF KARNAL BUNT

2021 ◽  
Vol 9 (12) ◽  
pp. 147-154
Author(s):  
Guillermo Fuentes-Davila ◽  
◽  
Ravi Prakash-Singh ◽  
Ivon Alejandra Rosas-Jauregui ◽  
Carlos Antonio Ayon-Ibarra ◽  
...  

The reaction to Tilletiaindica of one thousand and ninety twobread wheat advanced lines were evaluated in the field during the crop season 2016-2017. Sowing in beds with two rows was carried out on November 11 and 24, 2016, using 8 g of seed. Five spikes per line were inoculated by injection with 1 mL of an allantoidsporidial suspension (10,000/mL) during the boot stage, and at maturity the percentage of infection was determined by counting healthy and infected grains. The range of infection in the first date was 0-88.83 with a mean of 31.81%, while in the second date it was 0-82.65% with a mean of 24.44%.The range of infection of the two dates was 0.46-83.71% with a mean of 28.12%.Sixteenlines showed a percentage of infection equal or below 5.0% in both dates, and out of those lines, the following five showed less than 2.5%: two sister lines of MUNAL#1/FRANCOLIN#1*2/3/ATTILA*2/PBW65//MURGA(CMSS12Y00701T-099TOPM-099Y-099M-0SY-13M-0WGY), MUNAL#1/FRANCOLIN#1*2/3/ATTILA*2/PBW65//MURGA (CMSS12Y00701T-099TOPM-099Y-099M-0SY-17M-0WGY), BAJ#1/3/KIRITATI//ATTILA*2/PASTOR*2/4/MUTUS*2/TECUE#1, VILLAJUAREZF2009/6/ATTILA/3*BCN//BAV92/3/PASTOR/4/TACUPETOF2001*2/BRAMBLING/5/PAURAQ, and KACHU/BECARD//WBLL1*2/BRAMBLING/4/FRET2/TUKURU//FRET2/3/MUNAL#1. Lines with the highest percentage of infection were: BABAX/LR42//BABAX*2/3/KUKUNA/4/CROSBILL#1/5/BECARD/6/KSW/SAUAL//SAUAL/7/BABAX/LR42//BABAX*2/3/KUKUNA/4/CROSBILL#1/5/BECARD with 88.83 in the first date,MUU/KBIRD//2*KACHU/KIRITATIwith 84.77 and 82.65%in the first and second date, respectively, and TACUPETOF2001*2/BRAMBLING//WBLL1*2/BRAMBLING/6/WBLL1*2/KURUKU*2/5/REH/HARE//2*BCN/3/CROC_1/AE.SQUARROSA(213)//PGO/4/HUITES/7/BAV92//IRENA/KAUZ/3/HUITES/4/2*ROLF07 with 81.67% in the first date. The average of the three highest levels of infection of the susceptible checkwas99.7%.

2021 ◽  
Vol 2 (2) ◽  
pp. 1580-1589
Author(s):  
Guillermo Fuentes-Dávila ◽  
Ivón Alejandra Rosas-Jáuregui ◽  
Carlos Antonio Ayón-Ibarra ◽  
José Luis Félix-Fuentes ◽  
Pedro Félix-Valencia

Twenty advanced lines of triticale were evaluated for resistance to karnal bunt during the 2014-2015 crop season, at the Norman E. Borlaug Experimental Station. The sowing dates were November 19 and 29, 2014. One mL of an allantoid sporidial suspension (10,000/mL)  was injected during the boot stage of ten spikes per line. A mist-irrigation system was used to provide high humidity in the experimental area. Harvesting and threshing were done manually, and the counting of infected and healthy grains was perfomed by visual inspection. The range of infection for the first sowing date was 0 - 2.4%, with an average of 0.3, and for the second 0 - 1.4%, with an average of 0.4. Five lines did not show any infected grains in both dates and the rest was in the  0.1-2.5% infection category. The average of infection of the susceptible check during the period of inoculation of this group of lines was 86.6%. The highest average percentage of infection of both dates were shown by lines: TURACO/CENT.SARDEV/7/LIRON_2/5/DISB5/3/SPHD/ PVN//YOGUI_6/4/KER_3/6/BULL_10/MANATI_1/8/LIRON_2/5/DISB5/3/SPHD/PVN// YOGUI_6/4/KER_3/6/BULL_10/MANATI_1/9/BICEN (CTSS08Y00035S-099Y-026M-19Y-099M-2Y-2BMX-4Y)  with 1.6 and POPP1_2/CAAL//THELIN#2/5/PRESTO//2*TESMO_1/ MUSX603/4/ARDI_1/TOPO1419//ERIZO_9/3/SUSI_2/6/ARDI/GNU//2*FAHAD_1/4/BULL_ 10/MANATI_1/3/ELK54/ BUF_2//NIMIR_3 with 1.3%. These lines also showed the highest percentage of infection in the first date with 1.8 and 2.4%, respectively.  


2001 ◽  
Vol 81 (3) ◽  
pp. 503-508 ◽  
Author(s):  
D. A. Gaudet ◽  
G. Fuentes-Davila ◽  
R. M. De Pauw ◽  
P. A. Burnett

The reactions of 47 Canadian wheat and triticale cultivars to Tilletia indica Mitra, the causal agent of Karnal Bunt (KB), were evaluated in the field at Centro de Investigaciones Agricolas del Noroeste (CIANO), Yaqui Valley, Mexico, during 1998 and 1999. The majority of lines and cultivars flowered during January and February, which coincided with the onset of cooler temperatures and high relative humidity required for optimum infection by the fungus. Canada Western Amber Durum (CWAD) and triticale were resistant, ranging from 0 to 6% infection. Canada Western Red Spring (CWRS) wheats were also generally resistant, but the cultivars Roblin, BW90 and Laura, and the experimental line Roblin*2/BW 553, were susceptible, ranging from 11 to 28% in treatments with the highest infection levels. Canada Western Extra Strong (CWES) wheats varied in reaction from moderately resistant to susceptible, ranging from 1 to 15% infection. Canada Prairie Spring (CPS) wheats were generally susceptible, exhibiting infection levels ranging from 5 to 31% infection in the most severely infected treatments. Canada Western Soft White Spring (CWSWS) wheats were uniformly susceptible; the highest recorded infection level among Canadian wheats was observed on AC Reed (38%) during 1999. The susceptibility of CPS and CWSWS cultivars may represent an increased risk to the establishment of KB if the fungus were to become introduced into western Canada. Key words: Karnal bunt, wheat, disease resistance, disease susceptibility


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
HMT Herath ◽  
WHMW Herath ◽  
SO Duke ◽  
M Tomaso-Peterson ◽  
NPD Nanayakkara

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
JM Rollinger ◽  
R Spitaler ◽  
M Menz ◽  
P Schneider ◽  
EP Ellmerer ◽  
...  

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