Comparison of Harvesting Techniques and Corpus Luteum Bearing on Recovery and Quality of Sheep Oocytes

ABSTRACT: The objective of this study was to evaluate the sperm quality obtained of domestic cats by electroejaculation and recovery of the tail of the epididymis after cooling at -1°C and 4°C for 24 and 48 hours. Twenty-nine adult cats (2 to 6kg) were used. Sperm collection was performed by electroejaculation (EEJ), and after 48 hours, the cats were orchiectomized, and sperm sample was obtained from the vas deferens and epididymis tail (EPD). The samples were diluted in ACP-117® extender, and the sperm characteristics were evaluated at three different moments: when still fresh, 24 and 48 hours after cooling. In order to compare the two refrigeration temperatures, the first stage was to analyze if there was a difference between the harvesting techniques. After this, two experiments were conducted: in the first, sperm sample from 14 cats were used and the cooling was performed at -1°C; and in the second, sample from 15 cats were used and the sperm were refrigerated at 4°C. Sperm kinetics were evaluated by computerized analysis (CASA) and concentration by Neubauer chamber, spermatic morphology was evaluated by modified Karras staining, and membrane integrity was evaluated by eosin nigrosine. The results obtained were analyzed in R software, version 3.2.5 using the Mann-Whitney test for variables with abnormal distributions, considering significance at the level of 5%. In ejaculate samples, higher values of total morphological defects were observed after 24 and 48 hours of refrigeration at 4°C (P<0.022) compared to refrigeration at -1°C, using Friedman test. To quantify the decrease in sperm quality, parameter reductions were calculated among time points (F-24h/F-48h/24h-48h). In EPD samples, a greater reduction in sperm quality was detected after 24 hours of refrigeration at 4°C, both in motility and sperm kinetics and in the movement and velocity indices, compared to refrigeration at -1°C. Based on the results, it can be concluded that cooling of feline spermatozoa at -1°C for up to 48 hours was efficient in maintaining spermatic quality collected by EEJ and EPD, and it could be an alternative to spermatozoa cryopreservation in domestic felines.

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Comparative seasonal assessment on the quality of Black Bengal goat oocytes in view of in vitro embryo production

Asian-Australasian Journal of Bioscience and Biotechnology ◽

10.3329/aajbb.v5i3.53870 ◽

2020 ◽

Vol 5 (3) ◽

pp. 78-87

Author(s):

Shikha Sarkar ◽

Md Saiful Islam ◽

Md Enayet Kabir ◽

Falguni Dadok ◽

Md Abdullah Al Zaber ◽

...

Keyword(s):

Corpus Luteum ◽

Recovery Rate ◽

In Vitro Maturation ◽

Winter Season ◽

Excellent Opportunity ◽

Different Seasons ◽

In Vitro Embryo Production ◽

Black Bengal Goat

In vitro maturation (IVM) of oocytes provide an excellent opportunity for cheap and abundant embryos for carrying out animal improvement. With the aim for studying the quality of oocyte in different seasons of Black Bengal goat, both right and left ovaries were collected from the slaughter houses. For each of the specimens, gross parameters such as right, left, corpus luteum (CL)-present and corpus luteum (CL)-absent group were evaluated on the basis of weight (g), length (cm), width (cm), follicles aspirated and number and state of cumulus-oocyte-complexes (COCs), normal COCs and abnormal COCs, the effect of season on cumulus oocyte complexes (COCs) of goat oocytes. Our study revealed that the average number of follicles and oocytes recovery rate were higher in summer than in winter season and recovery rate of grade A and B oocytes were higher in summer than that of in winter and recovery rate of grade C and D were higher in winter than that of in summer. In the present study a significantly greater number of oocytes per ovary were recovered from ovaries without a corpus luteum than from ovaries with a corpus luteum. So the summer was the best season for recovery of COCs which have a worthy competence to be matured in vitro. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (3), 78-87

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Effect of follicular ablation and gonadotropin priming on the recovery and quality of oocytes in Boran cows

International Journal of Veterinary Science and Research ◽

10.17352/ijvsr.000093 ◽

2021 ◽

pp. 138-143

Author(s):

Demissie Tilaye ◽

Yilma Tefera ◽

Degefa Tamrat ◽

Wirtu Gemechu ◽

Lemma Alemayehu

Keyword(s):

Corpus Luteum ◽

Divided Dose ◽

Recovery Rate ◽

Oocyte Quality ◽

Cleavage Rate ◽

Exogenous Hormones ◽

Maturation Rate ◽

The Mean ◽

Possible Cause

Genetic differences have been suggested as a possible cause for variation in responses to exogenous hormones. Here we evaluated the effect of follicle ablation, exogenous FSH and coasting time prior to ovum pick-up (OPU) on the number of follicles suitable for aspiration, oocyte quality, and cleavage rate in Ethiopian Boran cows. The experiment was carried out in three parts, I) Cows were synchronized using 500µg PGF2α given 11 days apart. Cows were then subjected to a biweekly ovum pickup session before ovulation (n=5) or starting Day 7 after ovulation (n=4) for three weeks. II) Cows were similarly synchronized and all visible follicles were ablated on the first days of overt estrus which were then further grouped into cows that received a divided dose of 350IU FSH (n=5) or 175IU FSH (n=5) over three days. In both groups OPU was carried out weekly starting 48h after the last FSH for six weeks. III) A similar protocol as in part II was carried out but coasting period was increased to 72hrs for cows that received 350IU FSH as divided dose (n=5) and 48hrs coasting period for single 350IU FSH dose (n=5). The covariates of follicles and oocyte were not affected (P>0.05) by corpus luteum presence at OPU. The mean number of medium (7.36±0.57) and large (8.28±0.96) follicles were significantly higher (P<0.05) in group that received divided 350IU FSH. Similarly, the mean number of Grade-1 (4.19±0.24) and Grade-2 (4.32±.27) COC, maturation rate (70.41%) and cleavage rate (47.5%) were significantly higher (P<0.05) in group that received 350IU FSH. COC quality was significantly (P<0.05) influenced by costing period. However, both maturation and cleavage rates were not affected by the coasting period. This study demonstrated that follicular ablation and treatment with FSH improves follicular population and oocyte recovery rate in Boran cows.

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The Diversity Of Weeds In Organic Linseed And Flax Crop

Environment Technology Resources Proceedings of the International Scientific and Practical Conference ◽

10.17770/etr2011vol2.974 ◽

2015 ◽

Vol 2 ◽

pp. 276

Author(s):

Elvyra Gruzdevienė ◽

Zofija Jankauskienė

Keyword(s):

Research Centre ◽

Local Market ◽

Organic Farms ◽

Fibre Flax ◽

Experimental Station ◽

The World ◽

Harvesting Techniques ◽

Growing Conditions ◽

Cold Pressed

The flax is grown in the world for many years. The area of linseed in the world is much more than that of fibre flax. The seeds of ecologically grown linseed have high value as the row material for food, medicine, fodder, oil production. The cold pressed oil and seeds of ecologically grown linseed are especially popular in EU, Canada and USA. The quality of the finished linen product is often dependent upon growing conditions and harvesting techniques. The organic textile trend is starting to develop worldwide, while in Lithuania it is still almost non-existent. Therefore, the chance for Lithuanian farmers appears to export the ecological seed and fiber, not only use them in local market. Lithuanian farmers are in luck for the advices how to grow flax in ecological way. Therefore, in 2007- 2009 some investigations were carried out at the Upytė Experimental Station of the Lithuanian Research Centre for Agriculture and Forestry (Panevėžys district, Lithuania). The results of our investigation showed that it is possible to grow and harvest fibre flax and linseed in organic farms without any pesticides. The incidence of weeds is one of the biggest problems in organic growing of flax and linseed.

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Effects of age and quality of thawed embryos, synchronization and corpus luteum function on pregnancy rates of bovine embryo recipients

Theriogenology ◽

10.1016/0093-691x(87)90117-8 ◽

1987 ◽

Vol 27 (1) ◽

pp. 240 ◽

Cited By ~ 3

Author(s):

P. Humblot ◽

J. Perrin ◽

N. Jeanguyot ◽

M. Nibart ◽

M. Thibier

Keyword(s):

Corpus Luteum ◽

Pregnancy Rates ◽

Bovine Embryo

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Effect of ovarian types and collection techniques on the number of follicles and the quality of cumulus-oocyte-complexes in cow

Bangladesh Journal of Animal Science ◽

10.3329/bjas.v45i3.31034 ◽

2017 ◽

Vol 45 (3) ◽

pp. 10-16

Author(s):

Khandoker MAMY ◽

NF Atiqah ◽

N Ariani

Keyword(s):

Corpus Luteum ◽

Recovery Rate ◽

Normal Basis ◽

Blunt Dissection ◽

Embryo Production ◽

Aspiration Technique ◽

In Vitro Embryo Production ◽

Dissection Technique

This experiment was conducted for evaluation of bovine slaughterhouse ovary, follicles, and cumulus-oocyte-complexes (COCs) and to compare the effect of collection techniques on the recovery rate of COCs. The collected slaughterhouse ovaries was classified as corpus luteum present (CL+) and corpus luteum absent (CL-) groups. It was found that 62.5% of the ovaries collected were CL- type and 37.5% were CL+ type. For collection of COCs, blunt dissection and aspiration techniques were performed and number of follicles collected was recorded. The higher number of follicles were dissected and aspirated from CL - ovaries (11.2±1.8and 37.8±14.9, respectively) compared to CL+ ovaries 10.5±1.5and 28.3±15.6, respectively). The follicular materials collected from both techniques were observed under microscope to categorize the COCs as A (oocyte surrounded with cumulous cells homogenously), B (oocyte surrounded with cumulous cells partially), C (oocyte not surrounded at all by cumulous cells) and D (degeneration observed both in oocyte and cumulous cells). Grade A and grade B were classified as normal and grade C and grade D were considered as abnormal COCs. The result indicated that ovaries having no CL contributing more total number of COCs per ovary (6.8±1.0) and also contributing higher normal COCs (5.7±0.9) than that of ovaries with CL (6.0±2.0 and 4.5±1.5, respectively) in blunt dissection technique. But same trend of result was not found in aspiration technique. Similarly, higher percentage of COCs recovery rate was also recorded in blunt dissection (61.6±4.6% vs 16.5±4.9%, on total basis) than aspiration (48.6±2.9% vs 11.7±4.1%, on normal basis) technique. So, ovaries without CL and blunt dissection technique found more suitable for harvesting the higher number and superior quality of COCs for extending the in vitro embryo production experiment.Bang. J. Anim. Sci. 2016. 45 (3): 10-16

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Pesisir cattle superovulation with various dosage of Follicle Stimulating Hormone (FSH) on embrio production

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/888/1/012034 ◽

2021 ◽

Vol 888 (1) ◽

pp. 012034

Author(s):

T Afriani ◽

E Purwati ◽

J Hellyward ◽

Jaswandi ◽

M Mundana ◽

...

Keyword(s):

Experimental Design ◽

Corpus Luteum ◽

Analysis Of Variance ◽

Embryo Quality ◽

Follicle Stimulating Hormone ◽

Research Material ◽

Randomized Design ◽

Completely Randomized Design ◽

Stimulating Hormone

Abstract This study aimed to determine the use of various doses of FSH hormone, the number of lutheum corpus and the number and quality of embryos produced in the superovulation program in Pesisir cattle. The experimental design was Analysis of Variance (Anova) in a completely randomized design. The research material used 16 cows from Pesisir cattle that had been synchronized with estrous with CIDR implants for 13 days and were injected with the PGF2α hormone after the release of CIDR, then scheduled AI was performed. The dose of FSH hormone was 16 ml, 17 ml, 18 ml and 19 ml, each treatment consisting of 4 replications. The injection of the FSH hormone was carried out for 3 days at a decreased dose. Collection of donor embryos was carried out on days 6 to 8 after the scheduled AI. The parameters observed included superovulation response, number of corpus luteum, number of embryos and quality of embryos. The results showed that the response of Pesisir cattle superovulation was 81.25%. The average number of CL obtained was 13.75 and embryos for Pesisir cattle was 11.25. The results also showed that the quality of the embryos obtained were grade A 19, grade B 18, grade C 7, grade D 1. It can be concluded that the use of various doses of FSH hormone at doses of 16 mg, 17 ml, 18 ml and 19 ml had shown a superovulatory response. The 17 ml dose of FSH hormone showed a large number of embryos and had the best embryo quality.

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Influence of corpus luteum and ovarian volume on the number and quality of bovine oocytes

Animal Science Journal ◽

10.1111/asj.12261 ◽

2014 ◽

Vol 86 (2) ◽

pp. 148-152 ◽

Cited By ~ 7

Author(s):

Jurandy Mauro Penitente-Filho ◽

Carolina Rodrigues Jimenez ◽

Adriana Moreira Zolini ◽

Erly Carrascal ◽

Jovana Luiza Azevedo ◽

...

Keyword(s):

Corpus Luteum ◽

Ovarian Volume ◽

Bovine Oocytes

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Diagnosis of Interventional Transvaginal Maternal Diseases Based on Color Doppler Ultrasound

Journal of Healthcare Engineering ◽

10.1155/2021/5517785 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Canliang Wen ◽

Lan Huang ◽

Hongye Jiang

Keyword(s):

Ectopic Pregnancy ◽

Corpus Luteum ◽

Doppler Ultrasound ◽

Color Doppler ◽

Color Doppler Ultrasound ◽

Clinical Analysis ◽

Clinical Value ◽

Inspection Method ◽

Ultrasound Technology

In recent years, with the development of color Doppler ultrasound technology in obstetrics, this noninvasive, direct, convenient, and sensitive inspection method has become one of the best methods to observe the fetal circulation in the uterus. This paper discusses the clinical value of using transvaginal color Doppler ultrasound in the differential diagnosis of ovarian corpus luteum disease and ectopic pregnancy disease. This paper selects 100 cases of ectopic pregnancy and 100 cases of pregnant corpus luteum as the experimental research objects. Clinical analysis of transvaginal color Doppler ultrasonography was performed on all patients. In the process of measuring the patient’s ectopic pregnancy, the size of the patient’s adnexal mass is mainly measured, and the blood flow spectrum is measured. The clinical choice of transvaginal color Doppler ultrasound method to distinguish ectopic pregnancy disease and corpus luteum pregnancy disease can play a significant value. It can be effectively diagnosed according to the type of disease, then effective methods can be studied for clinical treatment, the quality of life of patients with the two diseases can be significantly improved, and the clinical application value of color Doppler ultrasound can be improved.

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