Antibacterial, Antioxidant and Angiotensin-I Converting Enzyme Inhibition Effects of Margotia gummifera Extracts rich in Phenolic Compounds

2021 ◽  
Vol 17 ◽  
Author(s):  
Moufida Bouchekrit ◽  
Hocine Laouer ◽  
Yavus Selim Cakmak ◽  
Mohamed Hajji ◽  
Saber Boutellaa ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Methanolic Extract ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Diffusion Method ◽  
Antihypertensive Activity ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme

Objective: The current investigation was conducted to evaluate phenol and flavonoid contents, antibacterial, antioxidant and antihypertensive potentials of three extracts of Margotia gummifera aerial parts. Methods: The antibacterial effect was assessed using wells agar diffusion method against 11 strains. The antioxidant effect was evaluated using different methods, 2, 2-diphenyl-1-picrylhydrazyl, total antioxidant capacity, ferric reducing ability power, cupric reducing antioxidant capacity and β-carotene/linoleic acid bleaching assay. The antihypertensive activity was performed using hippuryl-histidyl-leucine substrates. Results: The highest yield was obtained by hydro-methanolic extract, 19.801 %, however ethyl acetate extract seemed to be rich on phenolic and flavonoid compounds comparing to other extracts, 822.14±69.10 mg GAE g-1 ext. and 117.28±1.80 mg RE g-1 ext., respectively. The high free radical scavenging activity was mentioned in ethyl acetate extract with IC50 of 48.820±1.25 µg/ml. The same extract showed better antioxidant activity in all tested procedures. In antibacterial activity, the hydro-methanolic extract exhibited moderate effect against all tested bacteria except Salmonella enterica and Enterococcus faecalis which gave 18±2.0 and 16±1.0 mm of inhibition, whereas, it seemed to be inactive towards two Gram-negative bacteria. Concerning antihypertensive activity, all extracts can inhibit the Angiotensin-I Converting Enzyme, but the potential effect was given by hydro-methanolic and aqueous extracts, 97.75 % and 96.65 %, respectively. Conclusion: It can be stated that bioactive compounds of Margotia gummifera extracts had different biological activities which confirm their therapeutic uses in traditional medicine.

scholarly journals Studies on Chemical Constituents and Biological Activities of an Endophytic Fungi from Magnifera indica L.

2015 ◽  
Vol 13 (1) ◽  
pp. 63-67
Author(s):  
Mozammel Haque ◽  
Mohammad Shoeb ◽  
Nilufar Nahar
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Diffusion Method ◽  
Published Data ◽  
Nmr Studies ◽  
Brine Shrimp Lethality Assay

Two compounds, ergosterol (1) and 4-hydroxy-hexadec-6-enoic acid methyl ester (2) were isolated from the ethyl acetate extract of the endophytic fungal strain labeled as MI-3, isolated from the leave of Magnifera indica L. The structures of the isolated compounds were elucidated by 1H NMR studies and comparing with published data. The crude ethyl acetate extract, three column fractions and ergosterol were tested for antimicrobial activity against five Gram-positive and eight Gram-negative bacteria and three fungi by disc diffusion method. The general toxicity and antioxidant activity of the parent extract, column fractions and ergosterol were also evaluated by using brine shrimp lethality assay and free radical scavenging assay, respectively. Low activities were observed in all cases. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21862 Dhaka Univ. J. Pharm. Sci. 13(1): 63-67, 2014 (June)

Peptides present in the non-digestible fraction of common beans (Phaseolus vulgaris L.) inhibit the angiotensin-I converting enzyme by interacting with its catalytic cavity independent of their antioxidant capacity

2015 ◽  
Vol 6 (5) ◽  
pp. 1470-1479 ◽  
Author(s):  
Diego A. Luna-Vital ◽  
Elvira González de Mejía ◽  
Sandra Mendoza ◽  
Guadalupe Loarca-Piña
Keyword(s):  
Phaseolus Vulgaris ◽  
Antioxidant Capacity ◽  
Common Bean ◽  
Antioxidant Activities ◽  
Common Beans ◽  
Converting Enzyme ◽  
Phaseolus Vulgaris L ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme

Pure peptides present in common-bean non-digestible fraction inhibited ACE by interacting with its catalytic cavity, and presented antioxidant activities.

scholarly journals Characterization and Antioxidant and Angiotensin I-Converting Enzyme (ACE)-Inhibitory Activities of Gelatin Hydrolysates Prepared from Extrusion-Pretreated Milkfish (Chanos chanos) Scale

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 346 ◽  
Author(s):  
Chun-Yung Huang ◽  
Yung-Hsiang Tsai ◽  
Yong-Han Hong ◽  
Shu-Ling Hsieh ◽  
Ren-Han Huang
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Sodium Dodecyl ◽  
Degree Of Hydrolysis ◽  
Fish Scale ◽  
Fish Gelatin ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme ◽  
Ace Inhibitory

Fish gelatin hydrolysates have been shown to possess various biological activities due to their unique Gly-Pro-Y and Gly-X-Hyp sequences. In the current study, fish gelatin was extracted from non-extruded milkfish scale (FSG1) or extrusion-pretreated milkfish scale (FSG2); extracted gelatins were hydrolyzed with different combinations of Flavourzyme and Alcalase to give four different hydrolysates, namely: FSGH1 (FSG1 hydrolyzed with Flavourzyme), FSGH2 (FSG1 hydrolyzed with Alcalase + Flavourzyme), FSGH3 (FSG2 hydrolyzed with Flavourzyme), and FSGH4 (FSG2 hydrolyzed with Alcalase + Flavourzyme). The extrusion-pretreatment process enhanced the extraction yield of gelatin from fish scale. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared (FTIR) analyses showed the extracts FSG1 and FSG2 possessed characteristics of gelatin. Moreover, the physicochemical characteristics of FSGH1–FSGH4 were examined by analyses of their degree of hydrolysis, amino acid composition, UV spectrum, FTIR spectrum, molecular weight, and RP-HPLC profile. Additional biological functional analyses showed that all of the studied gelatin hydrolysates FSGH1–FSGH4 possessed antioxidant activity dose-dependently as revealed by DPPH scavenging, ABTS scavenging, and reducing power analyses. In addition, FSGH2 and FSGH4 showed higher angiotensin-I-converting enzyme (ACE)-inhibitory activity as compared to FSGH1 and FSGH3. Taken together, FSGH2 and FSGH4 showed high antioxidant activity and potent anti-ACE activity. Due to the potential antioxidant and antihypertensive properties of FSGH2 and FSGH4, further research is needed to explore their possible use as natural supplementary raw materials in food and nutraceutical products.

scholarly journals Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity of Peptides Isolated from Carabao Mango (Mangifera indica) Flesh

KIMIKA ◽  
2021 ◽  
Vol 32 (1) ◽  
pp. 1-18
Author(s):  
Trisha Rojas ◽  
Jherome Co ◽  
Mia Clare Marie Bercansil ◽  
Lory Jane Dela Cruz ◽  
Lawrence Yves Uy ◽  
...  
Keyword(s):  
Mangifera Indica ◽  
Ace Inhibition ◽  
The Philippines ◽  
Antihypertensive Activity ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Inhibitory Peptide ◽  
Angiotensin I Converting Enzyme ◽  
Ace Inhibitory

Hypertension has been reported as the second major cause of morbidity in the Philippines. One of the mechanisms to control blood pressure is through the inhibition of the angiotensin I-converting enzyme (ACE). This study specifically focused on the determination of ACE inhibitory activities of peptides from ‘Carabao’ mango flesh of three shell colors (SC1, SC3, SC5). The bioactive peptides were obtained upon a series of extraction, purification, and enzymatic hydrolysis steps. Among the undigested and digested crude and purified samples, the highest in vitro ACE inhibition was exhibited by the three-hour digest of SC3 (83.28 ± 0.83%). The most prominent peak from the fractionation of the mixture of peptides in SC3 digest was evaluated for ACE inhibition, and the decrease in activity inferred the synergistic effect of the peptides in exhibiting the inhibitory function. This selected bioactive peptide was revealed to have alanine and phenylalanine as the components, which can possibly be AF – an ACE inhibitory peptide determined from in silico analyses. Meanwhile, assessment of the in vivo antihypertensive activity showed no significant results due to insufficiently administered doses of the samples. Overall, the measured activity of the ACE inhibitory peptides inferred the potential of mango as a functional food in dealing with hypertension.  

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