scholarly journals An experimental study to evaluate the antidotal activity of Neeli Moola (Indigofera tinctoria) Kalka w.s.r to haematological parameters in Vatsanabha (Aconitum ferox ) induced toxicity

2020 ◽  
Vol 5 (06) ◽  
pp. 98-101
Author(s):  
Sreelakshmi A ◽  
Shrinidhi R ◽  
Sudhakar Bhat ◽  
Sreejith K ◽  
Chaithra S. Hebbar
Keyword(s):  
Hematological Parameters ◽  
Drug Group ◽  
Haematological Parameters ◽  
Albino Rats ◽  
Test Drug ◽  
Test Results ◽  
Wistar Strain ◽  
One Way Anova ◽  
Indigofera Tinctoria ◽  
Antidotal Activity

Background: Vatsanabha is a potent cardiac poison comes under the category of Mahavisha with its main active principles aconitine, picraconine and pseudaconine. In the literatures there are descriptions about toxicants and in some traditional Malayalam textbooks antidotes has been explained under the concept “Prathyoushadha”. Neeli Moola has been mentioned as a Prathyoushadha for Vatsanabha poisoning in a renowned textbook „Visha Vaidya Jyotsnika‟. Hence to evaluate the antidotal activity in Vatsanabha poisoning, Neeli Moola Kalka has been used and the haematological parameters were analyzed to screen the results. Methods: Wistar strain albino rats were used in this study which were divided into 3 groups, normal control, toxic control and the test drug group. The duration of the study was 28 days .The data generated was mentioned as Mean±SEM. Difference among the groups was assessed by employing one way ANOVA followed by Dunnet‟s mul tiple „t‟ test. Results: Reversible action has been observed after the administration of Neeli Moola Kalka in the hematological parameters which has shown toxicity changes due to administration of Vatsanabha. Conclusion: Neeli Moola Kalka is having mild to moderate antidote effect in Vatsanbha induced toxicity.

scholarly journals An Experimental Study to Evaluate the Anti-Diarrheal Activity of Bhuvaneshwara Rasa in Wistar Albino Rats

2021 ◽  
pp. 27-35
Author(s):  
Simi Jose ◽  
Chaitra L.V
Keyword(s):  
Experimental Study ◽  
Castor Oil ◽  
Raw Materials ◽  
Life Time ◽  
Drug Group ◽  
Albino Rats ◽  
Test Drug ◽  
Wistar Albino Rats ◽  
Important Disease

Atisara (Diarrhea) is most commonly encountered disease of the present era, due to unhealthy and irregular habits. Atisara (Diarrhea) finds a place as important disease in individual’s life as everyone suffers from it at least once in life time. Bhuvaneshwara rasa is a unique herbo-mineral formulation explained in Bhaishajya ratnavali indicated for all kinds of diarrhea cases. Bhuvaneshwara rasa is a potent formulation having Saindhava, Triphala, Yamani, Bilva peshika and Gruhadhooma. Materials and methods: Raw materials were screened and collected and the formulation selected for the present study Bhuvaneshwara rasa was prepared accordingly. Anti-diarrheal activity of Bhuvaneshwara rasa was evaluated experimentally in albino rats. Experimental study was conducted in 3 groups of animals for anti-diarrheal study each. Anti-diarrheal study was done by castor oil induced diarrhoea and castor oil induced enter-pooling method. Bhuvaneshwara rasa (test drug) and Loperamide are effective in controlling diarrheal episodes. Bhuvaneshwara rasa has shown significant Anti-diarrheal activity in both Castor oil -induced diarrhea and castor oil induced enteropooling, test drug effective than control. Result: Bhuvaneshwara rasa was prepared according to SOP. Test drug group have shown effect experimentally. Conclusion: Bhuvaneshwara rasa is a good anti-diarrheal drug. It can be administered in all types of Atisara.

scholarly journals PHARMACOLOGICAL IMPACT OF EDIBLE MACROFUNGI D. SPATHULARIA AND S. COMMUNE ON HEMATOLOGICAL PROFILE OF ALBINO RAT

2021 ◽  
Vol 9 (03) ◽  
pp. 527-533
Author(s):  
Amar Kumar ◽  
◽  
Manoj Kumar ◽  
Rakesh Ranjan ◽  
M.P. Sinha ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Schizophyllum Commune ◽  
Haematological Parameters ◽  
Albino Rats ◽  
Albino Rat ◽  
Cell Parameters ◽  
North East India ◽  
North East ◽  
Wbc Count ◽  
The Impact

Hematological investigations had been seen as helpful instruments in diagnosing the condition of wellbeing of an individual, the impact of food consumed can be assessed utilizing these apprehensions. In addition, the distinctive blood cell parameters are likewise helpful in the appraisal of immunological status of the individual. The present work was aimed to assess the impact of aqueous extract of edible macrofungiDacryopinaxspathularia and Schizophyllum commune on hematological parameters of albino rats. The two experimental macrofungi have been reported to possess several pharmacological properties like antioxidant, anti-diabetic, antibacterial, hepatoprotective, nephroprotective efficacies, and these are also used as traditional dietary and medicinal sources especially by the local people of North-east India. But the impact of these two edible macrofungi on the haematological parameters is still not reported. The results of the present work revealed that on administration of D. spathularia extract (500 mg/kg BW), slight but significant increase in total WBC count, platelets, eosinophils and monocytes was observed. There was no significant change found in rest of the hematological parameters. On the other hand, the administration of S. commune extract (500 mg/kg BW) had resulted into slight but significant increase in total WBC count and eosinophils, and rest all hematological parameters had not changed significantly. Thus, the two edible macrofungi under study does not have any adverse effects on haematological parameters and hence they are safe and can be used as dietary and nutraceutical purpose.

scholarly journals Phytochemical evaluation and exploration of the hepatoprotective activity of 5 different formulations in CCL4 induced albino RATS

2016 ◽  
Vol 4 (1) ◽  
pp. 25
Author(s):  
Hardik Patel ◽  
Nilesh Patel ◽  
Janmejay Patel ◽  
Payal Patel ◽  
Apurva Patel
Keyword(s):  
Total Bilirubin ◽  
Statistical Significance ◽  
Hepatoprotective Activity ◽  
Damage Parameter ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Significant Activity ◽  
Test Drug ◽  
Liver Disorders ◽  
One Way Anova

<p><strong>Background:</strong> Hepatotoxicity and Liver disorders are chronic disorders due to different causes. It affects people in their prime of life, predominantly between the ages of 25-75 years with unpredictable courses. The different formulations are assumed to have significant activity in the treatment of the Liver disorders.</p><p><strong>Objective:</strong> The present study planned to evaluate the synergistic efficacy activity of the different formulations using CCL4 induced hepatotoxic model albino rats.</p><p><strong>Materials &amp; Methods:</strong> The Phytochemical analysis of the T.cordifolia and five different formulations were performed. The animals were divided into eight different groups of 6 animals each as CCl4 treated, Single Plant extracts treated and another different test drug treated groups except 1st group, which was treated with only normal saline. The drugs were administered orally, twice a day and continued for 20 days. On the last day, all the group of animals were treated with the 1ml/kg CCl4 I.P. The Statistical significance was assessed using One-way ANOVA.</p><p><strong>Results:</strong> It was observed that 5 different formulations, i.e. Herbolive Syrup, Hepatonej Syrup, Hepanej Capsule, Herbolive Capsule, and Hepatonej Capsule produced significant hepatoprotective effect on 21st day. All the Formulations have significantly reduced the elevated level of Total Bilirubin, Direct Bilirubin, SGPT, SGOT, and ALP level.</p><p><strong>Conclusion:</strong> The result reveals that all the Herbomineral formulations possess the better hepatoprotective activity compare to single T.cordifolia plant extract. It is due to synergistic action of the various plants and minerals used into the formulation which brings down the elevated liver damage parameter to almost normal level.</p>

Evaluation of anti-arthritic potential of Leonotis nepetifolia (L.)R.Br. against Freund’s adjuvant induced arthritis

2017 ◽  
Vol 2 (05) ◽  
Author(s):  
Reshmi Pushpan ◽  
Nishteswar Karra ◽  
Mukesh B. Nariya ◽  
Ashok V. K.
Keyword(s):  
Aqueous Extract ◽  
Dosage Form ◽  
Kidney Diseases ◽  
Albino Rats ◽  
Test Drug ◽  
Standard Drug ◽  
Wistar Strain ◽  
Whole Plant ◽  
Significant Difference ◽  
Weak Activity

Background: Leonotis nepetifolia (L.)R.Br. (LN) belonging to Lamiaceae family is a tall erect annual weed native to Southern India and tropical Africa used by tribals and folklore traditions in India for cough, fever, stomach ache, skin ailments, kidney diseases, rheumatism and dysmenorrhoea. The purpose of the study is to evaluate the anti-arthritic activity of the traditional dosage form( decoction) as used by the tribals in comparison to a modified dosage form(dry aqueous extract ) of whole plant of LN in experimental animal models. Materials and Methods: Thirty wistar strain albino rats were selected and randomly divided into five groups. Arthritis was induced by Freund’s complete adjuvant (FCA) and then treated with either the decoction of whole plant of LN or the dry aqueous extract for 30 days.The various parameters like paw volume, ponderal changes, serum biochemical parameters and histopathological changes were assessed. The data was analyzed by employing one-way ANOVA followed by Dunnet’s multiple‘t’ test for unpaired data to determine significant difference between groups at P>0.05. Results: In the present study it was observed that dry aqueous extract form of the test drug is having weak activity against primary oedema whereas decoction form did not show any effect on primary oedema. Both forms of test drug have comparable values as standard drug on 25th day in secondary oedema. Conclusion: The findings suggest the beneficial effect of the drug against chronic inflammation and inhibition of periarthritis and osteogenic activity.

In Vivo Anti-Anemic Effect of an Aqueous Root Extract of Phyllanthus Muellerianus (Kuntze) Exell in Model Rats.

2021 ◽  
Author(s):  
James Nyirenda ◽  
Gershom B. Lwanga ◽  
Kaampwe M. Muzandu ◽  
David K. Chuba ◽  
Gibson M. Sijumbila
Keyword(s):  
Plant Extract ◽  
Hematological Parameters ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Aqueous Root Extract

Abstract Ethnopharmacological relevanceAnemia is a very serious condition in Zambia. One of the plants that has been used traditionally is Phyllanthus muellerianus where different parts of shrub are used to treat a number of diseases in Zambian folklore medicine. Earlier studies have investigated medicinal properties of its aqueous root extracts. This study evaluated the effect of P. muellerianus roots on the hematological indices of albino rats and determined its phytochemical profile. Aim of the studyTo carry out phytochemical screening of the root extract and assess the ant-anemic effect of the aqueous extract on laboratory rats with tail-bled induced anemia Materials and MethodsThirty-six male albino rats placed in six groups were used for the study. The groups comprised the 100, 200, and 400 mg/kg plant extract, Ranferon (200 mg/kg) positive control, anemic non treated control and a normal (non-anemic) control. Anemia, induced through bleeding of the rats, was defined as hemoglobin (Hb) levels less than 12 g/dL. The anti-anemic potential of the plant was determined by comparing its effect on the hematological parameters of rats on treatment to that of the control group.ResultsAfter treatment, rats on the 400 mg/kg plant extract dose showed the greatest increase in the mean values for Hb, Packed cell volume (PCV) and RBC count were 43.3±1.2%, 15.4±0.3 g/dL and 6.3±0.3 x106 /mL respectively, when compared to the negative control group (P < 0.05). Phytochemical screening revealed positive results for alkaloids, flavonoids, saponins, glycosides, steroids, triterpenoids and tannins with varying amounts.Conclusions. The aqueous root extract of P. muellerianus was efficacious against anemia in a dose-dependent manner. The phytochemical compositions seem to be responsible for its hematopoietic properties. Thus, the root decoction of P. muellerianus is useful in alleviating anemia and the results lend credence to its use in traditional medicine in the management of anemia.

scholarly journals Protective role of eclipta alba against hyperlipidemia induced by high-fat diet in albino rats

2019 ◽  
Vol 10 (2) ◽  
pp. 1181-1184
Author(s):  
Satheesh Naik K ◽  
Gurushanthaiah M ◽  
Nagesh Raju G ◽  
Lokanadham S ◽  
Seshadri Reddy V
Keyword(s):  
High Fat Diet ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Underlying Mechanism ◽  
Protective Role ◽  
Albino Rats ◽  
High Fat ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Eclipta Alba ◽  
Wistar Strain

Eclipta Alba has been used in traditional and folklore medicine to treat Hyperlipidemia and hepatic disorders. The present study was aimed to investigate the Antihyperlipidemic and hepatoprotective potentials of Eclipta Alba in high-fat diet -induced Albino rats and to determine the underlying mechanism.  A total of 30 adult albino rats of Wistar strain weighing 165–215 g were utilized. Animals were treated with high-fat diet for 8 weeks followed by post-treatment of E. Alba for 1 week, 2 weeks, and 3 weeks, respectively. After 12 h of fasting on the last day of the experiment, serum blood samples were collected in EDTA vials and processed for biochemical analysis.  A significant decrease in levels of total cholesterol and triglycerides was noted on animals treated with E. alba compared to high-fat diet animals. Treatment of hypercholesterolemic rats with E. Alba showed a marked decrease of serum low-density lipoprotein (LDL) and very LDL cholesterol concentrations compared to the hypercholesterolemic rats. High-fat diet feeding worsened the levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase enzymes, whereas the same markers were significantly improved by supplementation with E. alba compared to the normal group.  E. alba acts as an antihyperlipidemic agent in hyperlipidemic conditions and helps for better health.

scholarly journals Antihyperglycemic Activity of The Ethanolic Extract of the Bark of Mitragyna Ciliata (MYCA) in Albino Induced Diabetic Rats

2021 ◽  
pp. 957-965
Author(s):  
PAYE Plenseh Diana ◽  
James McClain
Keyword(s):  
Glucose Level ◽  
Hematological Parameters ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Vascular Complication ◽  
Severe Side Effect ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Toxicological Effect ◽  
Treatment Of Diabetes

Background: Diabetes an epidemic that gives rise to an increased macro vascular complication mortality rate of 1.5 million annually, increasing by 2035. Treatment of diabetes is complicated and associated with a severe side effect.</p> <p>Objective: To evaluate the antihyperglycemic property of the ethanolic extract of the back of MYCA and its toxicological effect on the hematological parameters with histopathological analysis of selected organs of induced diabetic albino rats.</p> <p>Method: The ethanoic extract (1000 mg/kg bw and 500mg/kg bw) and glibenclamide (0.5mg/kg bw) were administered to diabetic induced rats.  The effect of the extract on the hematological was studied in the diabetic induced rats. Histopathological changes were observed in the liver of induced diabetic rats after the administration of the MYCA extract.</p> <p>Results: The anti-hyperglycaemic effect was observed at two different levels, for two different doses (1000mg/kg and 500mg/kg). The results also showed significant suppression of blood glucose level in glucose fed hyperglycaemic albino rats but showed no significantly suppressed glucose level in overnight fasted normoglycemic rats.</p> <p>Conclusion: The findings revealed that the ethanolic extract of MITRAGYNA CILIATA (MYCA) possess antihyperglycemic property. Besides, the extract can prevent the various complication of diabetes and improve some hematological parameters.

scholarly journals PENGENDALIAN NYAMUK AEDES SPP MENGGUNAKAN PERANGKAP ALAMIAH

2018 ◽  
Vol 7 (1) ◽  
pp. 95-99
Author(s):  
Deri Kermelita
Keyword(s):  
Control Group ◽  
Sugar Solution ◽  
Test Results ◽  
Control Group Design ◽  
Mosquito Trap ◽  
Post Test ◽  
Observation Cage ◽  
Palm Sugar ◽  
Primary Vector ◽  
One Way Anova

Aedes spp mosquito is the primary vector  or the main transmitter of dengue fever. it’s existence cause public health disturbing. Many research are being done to find ways of controlling mosquitoes, one of them by using a mosquito trap attractants. The purpose of this study was to determine the effectiveness of the addition of attractants to modified mosquito trap to the Aedes aegypti mosquitoes control. The study design using analytical methods with a "post-test only control group design". The number of mosquitoes that are used at each observation cage was 30, with 9 times  repetition.  One way ANOVA used to analized the data then followed by LSD test. Results modifications mosquito trap attractants addition of palm sugar solution mixed with yeast bread is more effective (ρ = 0.000) of the yeast mixed tape.

scholarly journals PENGARUH MODIFIKASI ATRAKTAN TERHADAP JUMLAH TELUR NYAMUK Aedes sp YANG TERPERANGKAP DI KELURAHAN KARANGPUCUNG KECAMATAN PURWOKERTO SELATAN KABUPATEN BANYUMAS TAHUN 2014

2015 ◽  
Vol 34 (2) ◽  
pp. 1-19
Author(s):  
Sovi Milasari ◽  
Mela Firdaust
Keyword(s):  
Research Design ◽  
Test Group ◽  
Hemorrhagic Fever ◽  
Active Role ◽  
Group Differences ◽  
Test Results ◽  
Development Goals ◽  
The Village ◽  
Static Group ◽  
One Way Anova

Millennium Development Goals (MDGs), which is about illuminating the sixth goal of HIV / AIDS,malaria and other infectious diseases. One infectious disease is a disease of Dengue Hemorrhagic Fever(DHF). The purpose of this study is to determine the effect of the modification attractant the number ofeggs of Aedes sp Karangpucung trapped in the Village, District of Purwokerto Selatan Banyumas2014.Pre-experimental research design is, this research design using the static group comparisondesign. Determining sampling at this research using inclusion criteria. 5 study subjects DHF casesin 2013 were located in the Village. Karangpucung. Data accumulation techniques is by usingquestionner. Data analysis using Kruskall Wallis test as an alternative to One Way Anova test.Kruskal-Wallis statistical test results obtained p = 0.007 ≤ α 0,05 mean there is a significantdifference between the number of eggs of Aedes sp trapped in each attractant. Then followedeach test group differences using nonparametric analysis with the U Mann Whitney testgroups 1 and 2 are significant (p = 0.631) meaning that there is no difference, groups 1 3 and 2 3groups was significant (p = 0.009) means there different.In conclusion, the effect of the modification there isattractant against Aedes sp Karangpucungtrapped in the Village District of Purwokerto Selatan Banyumas 2014. Suggestion submitted to thecommunity should play an active role in the activities PSN Plus 3M.

scholarly journals PENGARUH VARIASI KONSENTRASI EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP KARAKTERISTIK SEDIAAN SALEP DENGAN MENGGUNAKAN BASIS PEG 4000 dan PEG 400

2021 ◽  
Vol 2 (02) ◽  
pp. 7
Author(s):  
Tri Puji Lestari
Keyword(s):  
Leaf Extract ◽  
Homogeneity Test ◽  
Test Results ◽  
Peg 400 ◽  
Cell Tissue ◽  
Peg 4000 ◽  
Significant Difference ◽  
Ointment Formulation ◽  
The Difference ◽  
One Way Anova

ABSTRAK Daun Belimbing Wuluh (Averrhoa bilimbi L) memiliki kandungan tanin dan flavonoid. Kandungan Flavonoid dan Tanin yang terdapat dalam belimbing wuluh mampu memberikan efek untuk menyembuhkan kulit yang mengalami kerusakan jaringan sel akibat luka bakar. Ada beberapa cara untuk pemanfaatan Daun Belimbing Wuluh antara lain dengan dibuat dalam sediaan salep. Pada penelitian ini salep dibuat dengan variasi konsentrasi ekstrak daun belimbing wuluh sebesar 10%, 15%, dan 20%  mengguankan basis PEG 4000 dan PEG 400. Ekstrak daun belimbing wuluh diperoleh menggunakan metode maserasi dengan menggunakan etanol 70% sebagai pelarutnya. Salep yang sudah dibuat kemudian dilakukan uji karakteristik meliputi uji organoleptis, homogenitas, daya lekat dan uji daya sebar. Hasil uji organoleptis dan homogenitas di analisa secara deskrisptif kemudian hasil uji daya lekat dan uji daya sebar di analisa dengan menggunakan analisa statistik one-way ANOVA.  Hasil penelitian menunjukan bahwa salep berbentuk semipadat dengan warna hijau muda sampai hijau kehitaman. Hasil uji homogenitas di dapatkan untuk ketiga formula adalah homogen. Nilai daya sebar pada ketiga formula menunjukan perbedaan yang signifikan, pada hasil uji daya lekat dan pH didapatkan tidak ada perbedaan yang signifikan terhadap ketiga formulasi. Dengan demikian perbedaan konsentrasi zat aktif yang digunakan berpengaruh terhadap daya sebar sediaan salep ekstrak daun belimbing wuluh. Kata kunci: Averrhoa bilimbi L; Salep; PEG   ABSTRACT Carambola leaf Wuluh (Averrhoa bilimbi L) contains tannins and flavonoids. The content of flavonoids and tannins contained in starfruit able to give effect to heal skin damaged cell tissue from burns. There were several ways for  utilization  of  Averrhoa  bilimbi  L.  among  others,  made  some  preparations, especially ointment formulation. This study aims to formulate leaf extract ointment preparation starfruit (Averrhoa bilimbi L) with various concentrations of 10%, 15%, and 20% using the PEG 4000 and PEG 400 as a base. Leaf extract obtained from meserasi leaf Averrhoa bilimbi  L  with  70%  ethanol. The ointment that has been made is then subjected to characteristic tests including organoleptic tests, homogeneity, adhesion and spreadability tests. The results of the organoleptic test and homogeneity were analyzed descriptively, then the results of the adhesion test and the spreadability test were analyzed using one-way ANOVA statistical analysis. The results showed that the ointment was semisolid, light green to blackish green. The homogeneity test results obtained for the three formulas are homogeneous. The value of the spreadability of the three formulas showed a significant difference, the results of the adhesion and pH test showed no significant difference between the three formulations. Thus the difference in the concentration of the active substance used affects the spreadability of the starfruit leaf extract ointment. Keywords: Averrhoa bilimbi L; ointment; PEG

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