scholarly journals Trapping bacteria and fungi using microfluidic design

2022 ◽  
Vol 9 (1) ◽  
pp. 110-116
Author(s):  
Galon et al. ◽  

Escherichia coli and Candida auris are not easy to identify in laboratories without special technology. In this study, we have presented microfluidic designs for trapping bacteria and fungi. Two trapping chambers are designed using AutoCAD and the fluid dynamics of the bacteria and fungi are simulated using D. Schroeder’s Fluid Dynamics Simulation software. The designs are modified versions of a device that is constructed and simulated with numerical predictions, which include sizes and apertures in consideration of the specified microbe. The current designs take into account the exact dimensions of E. coli and C. auris under fluid flow and passive microfluidic technique, where actuation is based on geometry, is considered. The measurements of the design ensure that the species are to be trapped due to diffusion and ¬¬fluid dynamics. From the simulation, the stagnation is to be shown with its default setting, and approximation is done in its motion which is simulated in the two-dimensional space of the bacteria and fungi. The microfluidic designs will be useful during experiments in deciphering necessary information of the bacteria and fungi and will be a platform in modeling numerous biomedical assays and in the optimization of biophysical tools.

2020 ◽  
pp. 0309524X2092495
Author(s):  
Federico González Madina ◽  
Alejandro Gutiérrez ◽  
Pedro Galione

In this work, two-dimensional models of Savonius rotors are simulated using OpenFOAM® in order to predict the aerodynamic performance of small-scale vertical-axis wind turbines. The results are reported analyzing the aerodynamic performance and forces acting on the rotors. Power coefficient, [Formula: see text], is compared with experimental data for each operation point, and for three different geometries. Simulations with first- and second-order discretization schemes are carried out and compared, both quantitative and qualitative. Since usual grid dimensions result not to be suitable for simulations of Savonius rotors, an analysis of different domains is performed and compared. Finally, a set up for computational fluid dynamics simulation of two-dimensional Savonius rotors is proposed. The fluid–rotor interaction is analyzed and the vortex shedding is correlated with [Formula: see text] values and wake description.


2013 ◽  
Vol 661 ◽  
pp. 81-86
Author(s):  
Lei Wang ◽  
Huai Chen ◽  
Ting Ting Liu ◽  
Jun Yang Ji ◽  
Xue Hui Gan

In the molding process of the tri-component composite spinning, the flow control of each component plays a significant impact on extrusion swell. This paper simulates the extrusion swell of the tri-component fiber in different flow ratio of different components based on Polyflow fluid dynamics simulation software, and measures the extrusion swell ratio of the tri-component fiber in different flow ratio of different components through the experiment. Simulation and experimental results show that by adjusting the melt flow ratio of the various components, we can obtain the desired extrusion swell ratio, which can improve the performance of the tri-component fiber.


2014 ◽  
Vol 50 (1) ◽  
pp. 58-71 ◽  
Author(s):  
Rajib Kumar Saha ◽  
Madhumita Ray ◽  
Chao Zhang

The disinfection characteristics of an open channel ultra-violet (UV) disinfection reactor is investigated numerically. The computational fluid dynamics (CFD) model used in this study is based on the volume of fluid (VOF) method to capture the water–air interface. The Lagrangian particle tracking method is used to calculate the microbial particle trajectory and the discrete ordinate (DO) model is used to calculate the UV intensity field inside the reactor. A commercial CFD software package ANSYS FLUENT is used to solve the governing equations. Custom user defined functions (UDFs) are developed to calculate the UV doses. A post-processor is developed in MATLAB to implement the inactivation kinetics of the microbes. The post-processor provides the probabilistic dose distribution and reduction equivalent dose (RED) values achievable in the reactor. The numerical predictions are compared with available experimental data to validate the CFD model. A parametric study is performed to understand the effects of different parameters on disinfection performance of the reactor. The low/high dosed particle trajectories, which can provide an insight for hydraulic and optical characteristics of the reactor for possible design improvements, are identified.


Author(s):  
A. Verschoor ◽  
J. Frank ◽  
M. Radermacher ◽  
T. Wagenknecht ◽  
M. Boublik

The small (30S) subunit of prokaryotic ribosomes can assume any of a wide range of tilt positions on the specimen support. Correspondence analysis should make it possible to order views appearing in the electron micrograph according to the angle of tilt.231 individual windowed images from two micrographs showing negatively stained 30S subunits from E. coli ribosomes were subjected to multireference alignment. Correspondence analysis yielded six morphologically significant factors of variance. The second of these related to variations in stain concentrations, which are irrelevant at the level of gross morphology. The coordinates for each image in five-dimensional space (relating to factors 1,3,4,5, and 6) were subjected to a nonlinear mapping algorithm, which calculated an optimal two-dimensional map.The resulting distribution (Fig 1) consisted of two clusters, one of rightfacing, the other of left-facing views. Subaverages along the outer margin of the cluster on the left showed the particle in a range of typical views.


2011 ◽  
Vol 415-417 ◽  
pp. 1859-1862 ◽  
Author(s):  
Guo Jun Jin ◽  
Gui Qin Li ◽  
Yi Sun ◽  
Li Xin Lu ◽  
Song Lin ◽  
...  

Two-dimensional modeling and the computational fluid dynamics simulation are performed to investigate the deposition rate and the uniformity of the thin film under different power in PECVD reaction chamber using 13.56 MHz frequency. The results of the simulation show that as the power increased, the deposition rate of the thin film first increased gradually and then saturated, but the changes of power have little effect on uniformity.


2018 ◽  
Vol 49 (1) ◽  
pp. 20-31 ◽  
Author(s):  
Beren R Jackson ◽  
Sam M Dakka

Airfoil self-noise or trailing edge noise and shear noise were investigated computationally for a NACA 0012 airfoil section, focusing on noise mechanisms at the trailing edge to identify and understand sources of noise production using ANSYS Fluent. A two-dimensional computational fluid dynamics simulation has been performed for 0°, 8°, and 16° airfoil angles of attack capturing surface pressure contours, contours of turbulent intensity, contours of surface acoustic power level, vorticity magnitude levels across the airfoil profile, and x- and y-directional self-noise and shear noise sources across the airfoil profile. The results indicate that pressure gradients at the upper surface do increase as the angle of attack increases, which is a measure of vortices near the surface of the trailing edge associated with turbulence cease as the boundary layer begins to separate. Comparison of the turbulent intensity contours with surface acoustic power level contours demonstrated direct correlation between the energy contributed by turbulent structures (i.e. vortices) and the level of noise measured at the surface and within the boundary layer of the airfoil. As angle of attack is increased, both x and y sources have the same trends; however, y sources (perpendicular to the free-stream flow) appear to have a bigger impact as angle of attack is increased. Furthermore, as the angle of attack increased, shear noise contributes less and less energy further downstream of the airfoil and becomes dominated by noise energy from vortical structures within turbulence. The two-dimensional computational fluid dynamics simulation revealed that pressure, turbulent intensity, and surface acoustic power contours further corroborated the previously tested noise observations phenomena at the trailing edge of the airfoil.


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