scholarly journals ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE SP.) AND TAWAS UT (AMPELOCISSUS SP.) FROM CENTRAL KALIMANTAN AGAINST PROPIONIBACTERIUM ACNES

2019 ◽  
pp. 7-10
Author(s):  
SYAHRIDA DIAN ARDHANY
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanolic Extract ◽  
Moderate Activity ◽  
Zone Of Inhibition ◽  
Central Kalimantan ◽  
Weak Activity ◽  
Vitro Effect

Objective: The aim of the present study was to investigate phytochemical screenings and the in vitro effect antibacterial of BD (Eleutherine Sp.) and TU (Ampelocissus Sp.) against Propionibacterium acnes.Methods: The antibacterial activity was investigated against Propionibacterium acnes by well diffusion method.Results: Preliminary phytochemical screenings of BD ethanolic extract were found positive alkaloid, saponin, tannins, and steroid while TUpositive flavonoid, saponin, tannins, steroid, and triterpenoid. Antibacterial activity against Propionibacterium acnes of ethanolic extract BD with concentration 25 mg/ml and 50 mg/ml showed the zone of inhibition 3.23 mm and 7.8 mm with category weak activity while ethanolic extract TU with same concentration showed zone of inhibition 10 mm (weak activity) and 16.3 mm (moderate activity) which mean ethanolic extract TU have better antibacterial activity. A combination ethanolic extract of both with variant ratio showed a zone of inhibition 6.7 mm (1:1), 3.9 mm (1:2), and 3.63 mm (2:1).Conclusion: In this present study showed the highest potential antibacterial activity against propionibacterium acnes is an ethanolic extract of TU and The best ratio combination is 1:1. Furthermore, this study needs more research with variant concentration so that may be possible to be used as natural anti-acne formulations.

scholarly journals ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT BAWANG DAYAK (ELEUTHERINE BULBOSA (MILL.) URB) IN CREAM AGAINST PROPIONIBACTERIUM ACNES

2019 ◽  
pp. 1-4
Author(s):  
SYAHRIDA DIAN ARDHANY ◽  
SUSI NOVARYATIIN
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanolic Extract ◽  
Moderate Activity ◽  
Adhesion Test ◽  
Organoleptic Characteristics ◽  
Weak Activity ◽  
Dispersion Test

Objective: The aims of this present study were to formulate antiacne cream consisting ethanolic extract of bawang dayak and evaluate antibacterialactivity of cream on day 0 and day 7 to see stability activity and preparation.Methods: Cream formula of bawang dayak was evaluated organoleptic characteristics, homogeneity, pH, adhesion test, dispersion test, and in vitroantibacterial against Propionibacterium acnes.Results: The results of evaluate cream homogeneity were F3 and F4 on day 7 separate and non-homogen but all formula pH suitable for topicalapplication. On day 0, F1 and F2 shown antibacterial activity in category weak activity, F3 and F4 in category moderate activity, while on day 7 allformula have antibacterial activity in category weak activity.Conclusion: All cream formula potentials inhibit against P. acnes but this research must be improved both of preparation and stability activity.

Evaluation of Antibacterial Activity of Combined Plant Extract of Pyracantha crenulata and Zanthoxylum armatum

2020 ◽  
Vol 2 (29) ◽  
pp. 32-36
Author(s):  
Semwal Amit Negi Sweta
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Disk Diffusion Method ◽  
Zanthoxylum Armatum ◽  
Plant Extraction

Abstract-Medicinal plants represent an essential source of drugs and have played an important role in healthcaresystem.PyracanthacrenulataandZanthoxylum armatumhave been used as traditional medicine. The main aim of the study was to find the antibacterial activity of the selected plants against bacterialspecies:E.coliandPseudomonasaeruginosa. The solvents used for plant extraction were hexane, chloroform, ethanol and aqueous. The in vitro antimicrobial activity was performed by Agar disk diffusion method. The hexane and aqueous extracts showed moderate activity whereas theethanolicextractsshowedasignificantantibacterial activity. In the study Tetracycline was used as standard. The combined ethanolic extract of both the selected plant showed the synergistic effect on the bacterial strain tested. This leads to the conclusion that the combined effect can have possible application in the development of products as antimicrobial.

scholarly journals In-vitro screening of antihelmintic and antibacterial activity of malvastrum coromandelianum leaves

2021 ◽  
pp. 4-7
Author(s):  
Tirupathi Rao Annavarapu ◽  
Pragna Malavika B ◽  
Aravinda Swami P
Keyword(s):  
Antibacterial Activity ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
The Other ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Malvastrum Coromandelianum

The main objective of the work is to investigate the antithelmintic and antibacterial activity of the Malvastrumcoromandelianum leaves. The extract was tested for antithelmintic activity against adult Indian earthworm and also tested for antibacterial activity against the gram positive bacteriaS.aureus,B.subtilis and gram negative bacteria against E.Coli, P.aerugenosa, P.putida.The anthelmintic activity was observed at 100mg/ml with reference to standardAlbendazole(10mg/ml).The maximum antibacterial activity was observed in S.aureus at 500mg/ml with of zone of inhibition17 mm and next is S.aureus, the best antibacterial activity was observed against P.aerugenosa and P.putidawith 15 mm of zone of inhibition. The zone of inhibition of extract was compared with standard Gentamycin 25 µg/ml. The extract shows significant activity against the other bacterial pathogens. From the results, it was concluded that the ethanolic extract of Malvastrumcoromandelianum leaves have anthelmintic and antibacterial activity.

COMPARATIVE IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF PLUMBAGO ZEYLENICA

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (04) ◽  
pp. 53-55
Author(s):  
G.P Vadnere ◽  
◽  
A.R. Pathan ◽  
B.U Kulkarni ◽  
A.K. Singhai
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Pathogens ◽  
Bacterial Pathogen ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Gastrointestinal Disorders ◽  
Moderate Activity ◽  
White Variety ◽  
In Vitro Antibacterial Activity

Ethanolic extract of leaf, flower and root of Plumbago zeylenica L. (white variety) traditionally used in the treatment of gastrointestinal disorders were screened for antibacterial activity against bacterial pathogens Staphyllococcus aureus, Escerchia coli, Klebsiella pneumoniae, Enterobacter aurugens, Proteus vulgaris, Shigella sonnei, Salmonella typhi which are known to cause different type of gastrointestinal disorders. The results showed that leaf and root extracts exhibited strong antibacterial action at concentration of 2-8 mg/ mL of the bacterial pathogen tested. However, powdered flower extract showed moderate activity. Thus the present data reveals that leaves and flowers are equally potential for the treatment of bacterial pathogens, which causes intestinal catarrh, indigestion, and colic intestinal abscesses.

scholarly journals IN VITRO COMPARISON OF THE ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF AZADIRACHTA INDICA LEAVES WITH GENTAMYCIN, AMPICILLIN, NITROFURANTOIN, AND COTRIMOXAZOLE ON BACTERIAL PATHOGENS ISOLATED FROM URINARY TRACT INFECTION PATIENTS

2017 ◽  
Vol 10 (8) ◽  
pp. 72 ◽  
Author(s):  
Meenakshi Jindal ◽  
Chauhan S
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Ethanolic Extract ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Neem Leaves ◽  
Mean Diameter ◽  
The Mean ◽  
In Vitro Comparison

  Objective: In vitro comparison of the antibacterial activity of ethanolic extract of Azadirachta indica (neem) leaves with gentamycin, ampicillin, nitrofurantoin, and cotrimoxazole in bacterial pathogens isolated from urinary tract infection (UTI) patients.Methods: Ethanolic extract of neem leaves was prepared by the standard method. The antimicrobial activity against bacteria isolated from UTI patients was determined by agar well diffusion method and then mean zone of inhibition of neem extract was compared with a mean zone of inhibition of gentamycin, ampicillin, nitrofurantoin, and cotrimoxazole.Results: Among 200 samples which were included in the study, bacteria isolated were Escherichia coli (60%), Klebsiella pneumoniae (15%), Pseudomonas aeruginosa (11%), Enterococcus faecalis (5%), Proteus mirabilis (3%), and Staphylococcus aureus (6%). The result obtained was statistically analyzed by unpaired t-test. The difference in the mean diameter of the zone of inhibition between ethanolic extract of Azadirachta indica (neem) leaves and nitrofurantoin was statistically highly significant for K. pneumoniae (p<0.0001) and P. mirabilis (p=0.01) and insignificant for other bacteria. On comparing, the mean diameter of the zone of inhibition of ethanolic extract of A. indica (neem) leaves with ampicillin and cotrimoxazole, it was found to be statistically highly significant for all bacteria E. coli (p<0.0001), K. pneumoniae (p<0.0001), P. aeruginosa (p<0.0001) E. faecalis (p<0.001), and S. aureus (p<0.0001) expect P. mirabilis. The mean diameter of the zone of inhibition of ethanolic extract of A. indica (neem) leaves when compared with gentamycin was statistically highly significant for all the bacteria.Conclusion: The ethanolic extract of A. indica leaves has antibacterial activity. Antibacterial activity of ethanolic extract of A. indica leaves is comparable to nitrofurantoin for bacteria E. coli, P. aeruginosa, E. faecalis, and S. aureus. Ethanolic extract of A. indica leaves has similar antibacterial activity as ampicillin and cotrimoxazole for bacteria P. mirabilis. The antibacterial activity of gentamycin is less as compared to an ethanolic extract of A. indica leaves for all the bacteria.

scholarly journals Formulasi Sediaan Krim Anti Acne dan Uji Aktivitas Ekstrak Etanol Bawang Dayak (Eleutherine bulbosa (Mill.) Urb) terhadap Propionibacterium acnes

2019 ◽  
Vol 2 (2) ◽  
pp. 121-126
Author(s):  
Syahrida Dian Ardhany ◽  
Yunari Puspitasari ◽  
Yuyun Meydawati ◽  
Susi Novaryatiin
Keyword(s):  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Homogeneity Test ◽  
Moderate Activity ◽  
Adhesion Test ◽  
Physical Test ◽  
Weak Activity ◽  
Dispersion Test

Bawang dayak (Eleutherine bulbosa (Mill.)urb) is one of plants of Central Kalimantan. Empirically, Bawang dayak is used to treat skin problems, one of them is acne vulgaris, so that it is necessary to test the inhibition of ethanolic extract of Bawang Dayak against acne causing such as P.acnes.  In the pharmaceutical world there is still no research conducted on the manufacture of antiacne cream contain ethanolic extract of Bawang Dayak bulbs, so the researchers conducted it to improve the efficiency of the use of traditional medicine. The results of phytochemical screening showed that ethanolic extract of bawang dayak contained flavonoid, alkaloid, saponin, tanin and catechol with the inhibition zone classified as moderate activity at the concentration of 1% and weak activity at other concentrations (5%, 10% and 15%). Based on the evalution of cream preparations the results of the organoleptic test for antuacne cream have a dark brown color and distinctive odor, homogen for homogeneity test, adhesion test and dispersion test according to the requirements and the pH observation showed ph (6) suitable for topical application. Based on these results, the evaluation antiacne cream fulfills the physical test, but it needs to be developed further for stability and effectiveness of antiacne cream against the bacteria acnes causing.   

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

scholarly journals Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Séverine Boisard ◽  
Anne-Marie Le Ray ◽  
Anne Landreau ◽  
Marie Kempf ◽  
Viviane Cassisa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Positive ◽  
Weak Activity ◽  
Agar Dilution

During this study, thein vitroantifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains:Candida albicans, C. glabrata, andAspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains includingStaphylococcus aureus. Organic extracts showed a significant antifungal activity againstC. albicansandC. glabrata(MIC80between 16 and 31 µg/mL) but only a weak activity towardsA. fumigatus(MIC80= 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially againstS. aureus(SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC10030–97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

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