scholarly journals THE USE OF CARROT SEED OIL (DAUCUS CAROTA L.) TO FORMULATE NANOEMULGELS AS AN EFFECTIVE NATURAL SUNSCREEN AND SKIN ANTI-AGING

2022 ◽  
pp. 124-129
Author(s):  
ANAYANTI ARIANTO ◽  
HAKIM BANGUN ◽  
SUMAIYAH ◽  
CHRISTY NATASYA DWI YANTI PUTRI SIREGAR
Keyword(s):  
Phase Separation ◽  
Seed Oil ◽  
Physical Stability ◽  
Tween 80 ◽  
High Energy ◽  
Protection Factor ◽  
Daucus Carota L ◽  
Cycling Test ◽  
Different Temperatures ◽  
Carrot Seed

Objective: The purpose of this study was to develop a nanoemulgel containing vegetable oil of carrot seed oil as an effective natural sunscreen and skin anti-aging. Methods: Nanoemulgels containing 4% carrot seed oil were formulated in three formulas with different ratios of Tween 80 and Sorbitol and prepared by using the high-energy emulsification method. The nanoemulgels were determined for the organoleptic characteristic, globule size, pH, physical stability during storage for 12 w at three different temperatures (room, high and low temperature), centrifugation, and cycling test. The Sun Protection Factor (SPF) value was determined by UV spectrophotometric method and the effectiveness of anti-aging was evaluated by using a skin analyzer and the results were compared with sunscreen emulgel. Results: Nanoemulgel containing 4% carrot seed oil with a ratio of Tween 80 as surfactant and Sorbitol as co-surfactant 40 and 20 resulted in the smallest mean droplet size of 338.34 nm and the sizes were increased during 12 w of storage at room temperature but still in the nano size and this nanoemulgel did not show phase separation or still stable. These nanoemulgels were also stable after the centrifugation and cycling test. The emulgel preparation was not stable or showed phase separation after the centrifugation test. The SPF value obtained from the nanoemulgel was 20.28±0.22 and these values were higher than the sunscreen emulgel (13.94±0.27). The pore size, spot, and wrinkles of the volunteer skin were reduced after using the nanoemulgel containing 4% carrot seed. Conclusion: The sunscreen and skin anti-aging activity of nanoemulgel preparation containing 4% carrot seed oil with a ratio of surfactant Tween 80 and co-surfactant Sorbitol 40 and 20 were more effective compare with emulgel preparation.

scholarly journals Preparation and Evaluation of Nanoemulgels Containing a Combination of Grape Seed Oil and Anisotriazine as Sunscreen

2020 ◽  
Vol 8 (B) ◽  
pp. 994-999
Author(s):  
Anayanti Arianto ◽  
Desi Yet Lie Lie ◽  
Sumaiyah ◽  
Hakim Bangun Bangun
Keyword(s):  
Seed Oil ◽  
Physical Stability ◽  
Grape Seed ◽  
Protection Factor ◽  
Transmission Electron Microscopy Analysis ◽  
Cycling Test ◽  
Transmission Electron ◽  
Grape Seed Oil ◽  
Droplet Morphology

BACKGROUND: Grape seed oil contains Vitamin E which acts as skin antioxidant and natural ultraviolet (UV) absorbent and anisotriazine is used as chemical absorbent. Sun protection factor (SPF) value of the sunscreen and physical stability can be increased using a combination of grape seed oil and anisotriazine as sunscreen material and preparation by nanotechnology. AIM: The objective of this study was to prepare and evaluate physical stability and in vitro SPF value of sunscreen nanoemulgel containing grape seed oil and anisotriazine. METHODS: Nanoemulgels containing 4% grape seed oil and anisotriazine (1.6% and 3.2%) were formulated by adding 2% of Carbopol 940 gel to the optimized nanoemulsions formulation with a ratio of nanoemulsion and gel 4:1. The nanoemulgels were evaluated physical stability during storage for 12 weeks at variations of temperature, centrifugation, and cycling test. SPF values of nanoemulgels were determined by UV–visible spectrophotometric method and compared to emulgel. Droplet morphology observation of nanoemulgel using transmission electron microscope. RESULTS: The results of this study showed that sunscreen nanoemulgel containing 4% grape seed oil and 3.2% anisotriazine had average droplet size of 187.5 nm, physically stable during experiment for 12 weeks at variation of temperature and after centrifugation and cycling test, but the sunscreen emulgel showed a phase separation. The SPF of nanoemulgel containing a combination of 4% grape seed oil and 3.2%, nanoemulgel without anisotriazine, and emulgel formulation was 19.325 ± 0.232, 11.169 ± 0.113, and 11.913 ± 0.161, respectively. Transmission electron microscopy analysis of droplet morphology showed that this nanoemulgel formulation formed a spherical globule. CONCLUSION: The sunscreen nanoemulgel formulation containing combination of 4% grape seed oil and 3.2% anisotriazine more stable than sunscreen emulgel during experiment for 12 weeks at room temperature and showed the SPF value higher compared to emulgel containing 4% grape seed oil and 3.2% anisotriazine and nanoemulgel without anisotriazine.

scholarly journals Characteristics and Stability of Nanostructured Lipid Carrier (NLC) Aleurites Moluccana Seed Oil (AMS oil) Using Various Combinations of Beeswax and Oleum Cacao

2019 ◽  
Vol 9 (01) ◽  
pp. 94-97
Author(s):  
Tristiana Erawati M ◽  
Denara Asha Putri ◽  
Arum Sekar Maharani ◽  
Noorma Asha Putri ◽  
Widji Soeratri
Keyword(s):  
Thermal Cycling ◽  
Real Time ◽  
Seed Oil ◽  
Physical Stability ◽  
Extreme Temperature ◽  
Stability Test ◽  
Nanostructured Lipid Carrier ◽  
Thermal Cycling Test ◽  
Cycling Test ◽  
Aleurites Moluccana

This study aims to determine the effect of the combination of beeswax and oleum cacao on the characteristics and stability of nanostructured lipid carrier-aleurites moluccana seed oil (NLC-AMs oil). The combination ratio of beeswax-oleum cacao and AMs oil is 3:1 which total lipid 20%, while the ratio of beeswax-oleum cacao used were F1(100:0); F2 (50:50); F3 (25:75); and F4 (0:100). These preparations are made by the high shear homogenization method because the processing technique is relatively easier, faster and it is possible to get nanoparticle size. Then characterization and physical stability test (real time, thermal cycling test, and centrifugation) were carried out. The results of this study can be concluded that: 1) NLC-AMs oil with combinations of beeswax and oleum cacao has smaller particle size than those using only single solid lipids (oleum cacao or beeswax). 2) Increased concentration of oleum cacao in the NLC-AMs oil system increases its viscosity. 3) The ratio of beeswax and oleum cacao affects the recrystallization index of the NLC-AMs oil. The lowest recrystallization index is in the NLC-AMs oil with a combination of beeswax-oleum cacao in F2 (50:50). 4) The results of the real time stability test for 8 weeks of storage revealed the NLC-AMs oil system with a combination of solid fat beeswax and oleum cacao on F3 (25:75) had the best stability. 5) All formulas are not stable against extreme temperature changes (thermal cycling test) and shocks (centrifugation) indicated by system separation.

scholarly journals FORMULATION AND EVALUATION OF COSMETIC FOUNDATION USING EPIGALLOCATECHIN GALLATE AS A SUN PROTECTION

2020 ◽  
pp. 130-134
Author(s):  
EFFIONORA ANWAR ◽  
SHIFA RIZKAMIARTY
Keyword(s):  
Phase Separation ◽  
Skin Irritation ◽  
Physical Stability ◽  
Epigallocatechin Gallate ◽  
Facial Skin ◽  
Protection Factor ◽  
Hen’S Egg ◽  
Irritation Test ◽  
Uv Visible ◽  
Skin Irritation Test

Objective: The objective of the study was to obtain a lotion foundation using epigallocatechin gallate (EGCG) as an active ingredient designed with asun protection factor (SPF) value around 30 that can effectively protect facial skin from ultraviolet radiation (UVR) exposure and that is safe to use.Methods: In this study, we determine SPF value using UV–visible spectrophotometry at a wavelength between 290 and 320 nm. The preparationformula of the foundation was made with an EGCG concentration of 0.4%, a concentration which can yield the desired SPF value of about 30. Physicalstability was performed at low (4±2°C), ambient (25±2°C), and high (40±2°C) temperatures; cycling and centrifugation tests were also conducted.Safety was evaluated by eye irritation test using hen’s egg test on chorioallantois method and skin irritation test using the Draize and patch testsmethod.Results: The SPF values of 0.04% EGCG and lotion foundation containing 0.4% EGCG were 31.02±0.72 and 33.20±0.59, respectively. The results ofcycling and centrifugal tests indicated that lotion foundation showed an absence of crystals and lack of any phase separation between oil and waterphases. The physical stability test showed no significant changes for all parameters. Safety tests resulted in neither skin nor eye irritation.Conclusion: The EGCG foundation developed was physically stable with a good appearance and did not irritate the skin or eyes thus are safe to usealso can effectively protect skin against UVR exposure.

scholarly journals PHYSICAL STABILITY AND ANTIOXIDANT ACTIVITY ASSAY OF A NANOEMULSION GEL FORMULATION CONTAINING TOCOTRIENOL

2017 ◽  
Vol 9 ◽  
pp. 140
Author(s):  
Mentari Mayang Suminar ◽  
Mahdi Jufri
Keyword(s):  
Antioxidant Activity ◽  
Room Temperature ◽  
Inhibitory Concentration ◽  
Physical Stability ◽  
Tween 80 ◽  
Skin Damage ◽  
Activity Assay ◽  
Nanoemulsion Gel ◽  
Different Temperatures ◽  
The Stability

Objective: Tocotrienols have an antioxidant potential higher than that of tocopherols. Nanoemulsion gel can deliver tocotrienols into the skin toprevent skin damage caused by free radicals and improve the stability of the dosage form. The present study aimed to determine the physical stabilityand antioxidant activity of a nanoemulsion gel formulation containing tocotrienol.Methods: The tocotrienol nanoemulsion was made using tocotrienols, oleic acid, Tween 80, 96% ethanol, and propylene glycol. The gel base was madeusing a carbomer and triethanolamine. A physical stability test was conducted at three different temperatures, namely, low temperature (4±2°C),room temperature (27±2°C), and high temperature (40±2°C). The antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl methodfor determining inhibitory concentration (IC50) values.Results: Formula 1 demonstrated the best physical stability, with a pH of 6.2. The droplet size of the tocotrienol nanoemulsion gel was 596 nm, witha zeta potential value of −27.1 nm. The IC50 of the tocotrienol nanoemulsion gel was 6252.14 ppm.Conclusion: The nanoemulsion gel formulation retained antioxidant activity and was physically stable for 8 weeks.

scholarly journals Preparation and Evaluation of Sunscreen Nanoemulsions with Synergistic Efficacy on SPF by Combination of Soybean Oil, Avobenzone, and Octyl Methoxycinnamate

2019 ◽  
Vol 7 (17) ◽  
pp. 2751-2756
Author(s):  
Anayanti Arianto ◽  
Gra Cella ◽  
Hakim Bangun
Keyword(s):  
Phase Separation ◽  
Soybean Oil ◽  
Droplet Size ◽  
Room Temperature ◽  
Liquid Paraffin ◽  
Physical Stability ◽  
Antioxidant Properties ◽  
Ultra Violet ◽  
High Energy ◽  
Natural Oils

BACKGROUND: Soybean oil contains vitamin E and acts as a natural sunscreen which can absorb Ultra Violet (UV) B light and has antioxidant properties to reduce the photooxidative damage that results from UV-induced Reactive Oxygen Species production. The UV blocking from most natural oils is insufficient to obtain a high UV protection. The strategies for preparations of sunscreen products with high SPF can be done by nanoemulsion formulation and Ultra Violet filter combinations of Soybean Oil, Avobenzone and Octyl methoxycinnamate. AIM: The purpose of this study was to prepare and in vitro efficacy evaluation of sunscreen nanoemulsion containing Soybean oil, Avobenzone and Octyl methoxycinnamate. METHODS: The sunscreen nanoemulsions were prepared by the high energy emulsification method. The formulation uses a combination of 3% Avobenzone, 7.5% Octyl methoxycinnamate, with different ratio of Soybean oil and Liquid Paraffin. The nanoemulsion was evaluated for droplet sizes by using particle size analyzer, physical stability in room temperature (25 ± 2°C during experiment for 12 weeks of storage, physical stability (cycling test), phase separation by centrifugation at 3750 rpm for 5 hours, pH, viscosity, and Sun Protection Factor (SPF) value by UV spectrophotometric. The SPF value of sunscreen nanoemulsion was compared to sunscreen nanoemulsion without Soybean Oil and sunscreen emulsion. Particle morphology observation of nanoemulsion by using Transmission Electron Microscope. RESULTS: The sunscreen nanoemulsion formulation containing a combination of 3% Avobenzone, 7.5% Octyl methoxycinnamate with a ratio of 2.73% Soybean Oil and 0.27% Paraffin Oil resulted in the smallest average droplet size of 68.47 nm. The sunscreen nanoemulsion without Soybean Oil had an average droplet size of 384.07 nm. The globules size was increased during the experiment for 12 weeks of storage at room temperature, but there was no phase separation after centrifugation. The formulation of sunscreen emulsion, phase separation was formed after centrifugation. The nanoemulsion had a pH value of 7.23 ± 0.06 and a viscosity value of 133.33 ± 7.22 cP. The sunscreen nanoemulsion containing a combination of 3% Avobenzone, 7.5% Octyl methoxycinnamate 2.73%, Soybean Oil, 2.73% and 0.27% Liquid Paraffin had SPF value (21.57 ± 1.21) higher than sunscreen nanoemulsion without Soybean Oil (16.52 ± 0.98) and sunscreen emulsion (15.10 ± 0.22). The TEM analysis of globules morphology showed that the sunscreen nanoemulsion formed a spherical globule. CONCLUSION: The sunscreen nanoemulsion containing a combination of 3% Avobenzone, 7.5% Octyl Methoxycinnamate, 2.73% Soybean Oil and 0.27% Liquid Paraffin showed synergistic sunscreen efficacy on SPF. This sunscreen nanoemulsion is more stable than sunscreen emulsion formulation during the experiment for 12 weeks at room temperature.

scholarly journals Physical Stability and Sun Protection Factors Measurement of Sunscreen Preparations in Stress Storage Conditions Using Spectrophotometry

2018 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Widi Wulandari ◽  
Hendri Wasito ◽  
Sri Sutji Susilowati
Keyword(s):  
Ph Value ◽  
Physical Stability ◽  
Chemical Properties ◽  
Storage Condition ◽  
Storage Conditions ◽  
Sun Protection ◽  
Emission Region ◽  
Protection Factor ◽  
Different Temperatures ◽  
Value Measurement

Background: Sunscreen preparations can be used effectively to absorb sunlight especially in the UV wave emission region. The stress condition during the storage of preparation may affect physical and chemical properties. This study aims to determine the effect of stress storage conditions on physical stability and Sun  Protection  Factor (SPF) values as the effectiveness of sunscreen preparations stored in three different temperatures. Method : This experimental study was conducted with the selection of sunscreen samples and performing physical stability tests in termed of color observation, physical observation, pH value, spreading test, adhesive test, and SPF value measurement of sunscreen using spectrophotometry in lotion and gel dosage forms stored at room temperature (25◦C), low temperature (4◦C), and high temperature (40◦C). Results: The results showed that the stress storage condition influenced the physical properties of the preparation in the form of adhesive property, spreading capacity, and significantly affect the SPF value of the sunscreen (p <0.05) after 14 days of storage.

scholarly journals FORMULASI SEDIAAN KRIM A/M DENGAN VARIASI KONSENTRASI EKSTRAK ETANOL DAGING BUAH PEPAYA (Carica Papaya) MENGGUNAKAN EMULGATOR TWEEN 80 DAN SPAN 80

Media Farmasi ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 33
Author(s):  
Gita Puspita ◽  
Nining Sugihartini ◽  
Iis Wahyuningsih
Keyword(s):  
Phase Separation ◽  
Physical Properties ◽  
Carica Papaya ◽  
Physical Stability ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Test Results ◽  
Fruit Extract ◽  
Extract Concentration ◽  
Span 80

Papaya (Carica papaya) contains flavonoid and phenolic compounds proven to have sunscreen and antithyrosinase activity. For this reason, there is a need to formulate them in the form of cream preparations. This study analyzes the effect of the ethanol extract concentration of papaya flesh in the form of A / M cream preparations on their physical properties. The extracts were obtained using the maceration method with 70% ethanol solvent in A / M cream preparations and concentrations of 1%, 3%, and 5% through Tween 80 and Span 80 emulgators. Creams were evaluated for physical properties, including pH, adhesion, dispersion, viscosity, and physical stability. The test results showed that an increase in the concentration of ethanol extract led to a decrease in pH (p <0.05), viscosity (p <0.05), adhesion (p <0.05), and an increase in dispersion (p <0.05). Additionally, phase separation occurs at a concentration of 5% papaya fruit extract cream. These results show that variations in the ethanol extract concentration of papaya fruit influence the physical properties of A / M cream.Keywords: Carica papaya; A / M cream; Physical Properties.Pepaya (Carica papaya) mengandung senyawa flavonoid dan fenolik  yang terbukti memiliki aktivitas sebagai tabir surya dan antitirosinase sehingga perlu diformulasikan dalam bentuk sediaan krim. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh konsentrasi ekstrak etanol daging buah papaya dalam bentuk sediaan krim A/M terhadap sifat fisik sediaan. Ekstraksi buah papaya diperoleh dengan metode maserasi dengan pelarut etanol 70% dalam sediaan krim A/M dengan konsentrasi 1% (FI), 3% (F2) Dan 5% (F3). Krim dievaluasi sifat fisik meliputi pH, daya lekat, dan daya sebar, viskositas dan stabilitas fisik. Hasil uji menunjukkan bahwa peningkatan konsentrasi ekstrak etanol buah papaya menyebabkan penurunan pH (p<0.05), viskositas (p<0.05), daya lekat (p<0.05), peningkatan daya sebar (p<0.05). Terjadi pemisahan fase pada konsentrasi 5% krim ekstrak buah papaya. Berdasarkan hasil dapat disimpulkan bahwa variasi konsentrasi ekstrak etanol daging buah papaya memiliki pengaruh terhadap sifat fisik krim A/MKata kunci : Carica papaya; krim A/M; Sifat Fisik

scholarly journals Optimasi Formulasi Esens Sheet Mask Kombinasi Ekstrak Spirulina platensis dan Nanopartikel Bentonit dengan Metode Simplex Lattice Design

2019 ◽  
Vol 16 (1) ◽  
pp. 18-27
Author(s):  
Erindyah Retno Wikantyasning ◽  
Ulil Fikri Nurhakimah ◽  
Ramadhani Dwi Sula ◽  
Kartika Fidi Astuti
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Spirulina Platensis ◽  
Physical Stability ◽  
Tween 80 ◽  
High Energy ◽  
Lattice Design ◽  
Nanoparticles Characterization ◽  
Simplex Lattice ◽  
Optimum Formula

Spirulina platensis contained phycocyanin that has the potential for antioxidant activity. Bentonite contains various minerals that can absorb water and was known having ability for scavenging of free radicals. The purpose of this study was to obtain the optimum formulae of sheet mask essence, containing combination of Spirulina platensis extract and bentonite nanoparticles and evaluated the antioxidant activity using DPPH (2,2-diphenyl-1-pikrihidrazil) assay. Bentonite nanoparticles was prepared by High Energy Milling (HEM) method and characterized using Particle Size Analyzer (PSA). The results of the nanoparticles characterization was 152,8±0,69 nm for the particle size and 0.106±0.03 for PI value. Physical stability tests showed that the formula was homogenous, viscosity between 0.7-1.1 dPas, and pH between 5-6.5. The IC50 value of Spirulina platensis extract was 256.46 ppm with AAI category was medium. The antioxidant test of this fomula that contain Spirulina platensis extract and bentonite nanoparticles did not show any potential antioxidant activity. The concentrations of PEG 400 and Tween 80 were used to formulate the optimum formula were 4.29% w/w and 6.71% w/w.

scholarly journals Formulation and Evaluation of Moringa Seed Oil Nanoemulsion Gel

1970 ◽  
Vol 7 (6) ◽  
pp. 1-5 ◽  
Author(s):  
Nita Tirmiara ◽  
Julia Reveny ◽  
Jansen Silalahi
Keyword(s):  
Particle Size ◽  
Room Temperature ◽  
Seed Oil ◽  
Tween 80 ◽  
High Energy ◽  
Particle Size Measurement ◽  
Nanoemulsion Gel ◽  
Oil Concentration ◽  
Gel Preparation ◽  
The Stability

Objective: The objective of this study was to formulate and evaluate the moringa seed oil (MSO) nanoemulsion gel using high-energy emulsification method. Methods: Nanoemulsion gel formulated by high-energy emulsification method using the comparison of surfactant (tween 80) and cosurfactant (sorbitol) concentration with the variation of moringa seed oil concentration. Evaluation of the stability of the nanoemulsion gel preparation includes centrifugation test, viscosity, pH, organoleptic observation (odor, color, clarity, and phase separation), and particle size measurement during 12 weeks storage at room temperature. Results: The results showed that all nanoemulsion gel preparations are transparent yellow, characteristic odor, type weights 1.0888–1.1193 g/ml, and stable for 12 weeks storage at room temperature. The smallest particle size produced by the nanoemulsion gel preparation in a formula of the concentration of 5%, which 52.25 nm. Conclusions: Moringa seed oil can be formulated as a nanoemulsion gel by high energy emulsification method. MSO  with a 5% concentration was very stable for 12 weeks storage.    

scholarly journals Stabilitas Fisik Dan Antioksidan Mikroemulsi Minyak Nilam Dengan Variasi Tween 80 Dan PEG 400

Media Farmasi ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 185
Author(s):  
Ayu Shabrina ◽  
Annisa Rahayuning Pratiwi ◽  
Mimiek Muurukmihadi
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Tween 80 ◽  
Test Method ◽  
Test Results ◽  
Climatic Chamber ◽  
Cycling Test ◽  
Peg 400 ◽  
Pogostemon Cablin ◽  
Dpph Method

Patchouli oil (Pogostemon cablin Benth.) has potential as an antioxidant and high volatility due to air and light, therefore it needs to be formulated in the form of a microemulsion. This research aims to determine the physical stability and antioxidant microemulsion of patchouli oil with different concentrations of Tween 80 and PEG 400. Microemulsions was prepared with patchouli oil content of 5% along with different concentration of Tween 80: PEG 400 were  F1 (26%: 13%), F2 (13%: 26%) and for F3 (26%: 26%). The patchouli oil microemulsion was tested for physical stability by the cycling test method using a climatic chamber at a temperature of 40 oC ± 2oC and 4 oC ± 2oC  for 6 cycles. Furthermore, the tests include organoleptic, homogeneity, viscosity, pH, and separation. The antioxidant test was carried out using the DPPH method, and the quantitative data on physical stability and antioxidants were analyzed by a t-dependent test. The stability test results showed that F2 and F3 had decreased viscosity after 6 storage cycles (p <0.05). The results of the homogeneity, pH and separation tests did not experience significant differences. The test results of patchouli oil microemulsion antioxidant activity showed no change in antioxidant activity during 6 storage cycles with the mean inhibition percent before and after the cycling test was  73,13 ± 0,38 %.  Patchouli oil microemulsion with tween 80: PEG 400 26%: 13% showed the most stable physical quality and antioxidant properties.Keywords : Microemulsion, Patchouli Oil, Physical Stability, AntioxidantsMinyak nilam (Pogostemon cablin Benth.) berpotensi sebagai antioksidan dan memiliki laju volatilitas tinggi akibat udara dan cahaya sehingga perlu diformulasikan dalam bentuk mikroemulsi. Penelitian ini bertujuan untuk mengetahui stabilitas fisik dan antioksidan mikroemulsi minyak nilam dengan variasi konsentrasi tween 80 dan PEG 400. Mikroemulsi dibuat dengan kadar minyak nilam 5% dan variasi konsentrasi campuran tween 80:PEG 400 adalah  untuk F1 (26%:13%) dan F2 (13%:26%) serta untuk F3 (26%:26%). Mikroemulsi minyak nilam diuji stabilitas fisik dengan metode cycling test menggunakan climatic chamber pada suhu 40 oC ± 2oC dan 4 oC ± 2oC selama 6 siklus. Pengujian meliputi organoleptis, homogenitas, viskositas, pH, dan uji pemisahan. Uji antioksidan dilakukan menggunakan metode DPPH. Data kuantitatif dari stabilitas fisik dan antioksidan dianalisis dengan uji t-dependent. Hasil uji stabilitas menunjukkan F2 dan F3 mengalami penurunan viskositas setelah 6 siklus penyimpanan (p < 0,05). Hasil uji homogenitas,pH dan uji pemisahan tidak mengalami perbedaan bermakna. Hasil uji aktivitas antioksidan mikroemulsi minyak nilam menunjukkan tidak terjadi perubahan aktivitas antioksidan selama 6 siklus penyimpanan dengan rerata persen inhibisi sebelum dan sesudah cycling test adalah 73,13 ± 0,38 %. Mikroemulsi minyak nilam F1 dengan tween 80: PEG 400 26%:13% menunjukkan Mutu fisik dan sifat antioksidan yang paling stabil.Kata kunci : Mikroemulsi, Minyak Nilam, Stabilitas Fisik, Antioksidan

Sign in / Sign up

Export Citation Format

Share Document