scholarly journals Parvovirus B19 affects thrombopoietin and IL-11 gene expression in human bone marrow mesenchymal stem cells

2021 ◽  
Author(s):  
Mahin Behzadi Fard ◽  
Amir Atashi ◽  
Shahin Amiri ◽  
Saeid Kaviani ◽  
Mohammad Ali Gholampour ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Target Genes ◽  
Parvovirus B19 ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Gene Expressions ◽  
Marrow Mesenchymal Stem Cells

Aim: Human bone marrow mesenchymal stem cells (hBMSCs) may be infected by parvovirus B19 (B19V). hBMSCs support bone marrow hematopoiesis by producing stromal cells, secretion of cytokines and growth factors, etc. Because of the lifetime persistent infection of the virus in healthy individual’s bone marrow, this study aims to evaluate B19V effects on hBMSCs gene expression of some crucial hematopoietic cytokines. Materials & methods: hBMSCs were transfected with pHI0 plasmid containing the B19V genome. The quantitative mRNA expression of target genes was evaluated 24 h after transfection. Results: Our findings demonstrated a significant increase in expression levels of IL-11 and TPO (p < 0.05). Conclusion: We concluded that alteration in the gene expressions in B19V-infected hBMSCs might have significant effects on the bone marrow microenvironment as well as hematopoiesis.

scholarly journals The Effect of TNF-α on the Expression of MMP9 in Human Mesenchymal Bone Marrow-Derived Stem Cells

2020 ◽  
Vol 10 (1) ◽  
pp. 138
Author(s):  
Khaled Sharifi ◽  
Maryam Ayatollahi ◽  
Ramin Yaghoubi ◽  
Mohmmad Hossain Sanati ◽  
Afsune Afshari ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adult Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Control Group ◽  
Marrow Mesenchymal Stem Cells ◽  
Mmp9 Gene

Matrix metalloproteinase 9 (MMP9) as the enzyme of adult stem cells secreted from damage cells. In spite of low level of MMP9 enzyme in the mesenchymal stem cells, many inflammatory cytokines stimulation such as TNF-&alpha; could increase MMP9 level in cells. Current study evaluated the expression of the MMP9 enzyme under the influence of TNF-&alpha; in human bone marrow mesenchymal stem cells. The human bone marrow mesenchymal stem cells were classified into control and experimental groups. In the experimental groups, various concentrations of the TNF-&alpha; (1ng/ml and 10ng/ml) were administrated in different times (10 and 24 hours), whereas the control group was not treated with TNF-&alpha;. MMP9 gene expression was evaluated by Real-Time PCR. TNF-&alpha; administration in 1ng/ml and 10ng/ml dosage for 10 hours, induced the expression of MMP9 1468.3 and 1782.8 times more than the control group, respectively. After 24h, in comparison between 1ng/ml and 10ng/ml with control groups, MMP9 expression were 442.64 and 1184.4 times more than control group, respectively. In conclusion, the expression rate of the MMP9 gene in bone marrow mesenchymal stem cells might be effected by dosage and time of exposure to TNF-&alpha;. Furthermore, the time of exposure might have the prominent role in alteration of MMP9 gene expression induction in the mesenchymal stem cells.

scholarly journals Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 58 (1) ◽  
pp. 63-73
Author(s):  
Shahin Amiri ◽  
Amir Atashi ◽  
Mehdi Azad ◽  
Azin Elmi ◽  
Mahnoosh Abbaszade Dibavar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Inflammatory Cytokine ◽  
Parvovirus B19 ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cytokine Genes ◽  
Marrow Mesenchymal Stem Cells

Candidate kinases for adipogenesis and osteoblastogenesis from human bone marrow mesenchymal stem cells

2021 ◽  
Author(s):  
Xia Yi ◽  
Ping Wu ◽  
Jianyun Liu ◽  
Shan He ◽  
Ying Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factors ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cytoplasmic Proteins ◽  
Marrow Mesenchymal Stem Cells

Adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis) are closely related processes involving with the phosphorylation of numerous cytoplasmic proteins and key transcription factors.

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