scholarly journals Magnitude, Seasonal-variation, Serological and Hematological Profile of Dengue in a Tertiary Teaching Hospital, Karwar, India

2021 ◽  
Author(s):  
F. Sneha Kukanur ◽  
G. Naveen ◽  
N. Ashwin Chitrabanu ◽  
B.M. Prashant ◽  
R. Meghana ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Indian Subcontinent ◽  
Dengue Infection ◽  
Viral Disease ◽  
Antibody Detection ◽  
P Value ◽  
Igg Antibody ◽  
Tertiary Teaching ◽  
Ns1 Antigen ◽  
One Year

Dengue viral infection is the most widely spread arbo-viral disease in Indian subcontinent. High index of clinical suspicion especially during its peak season can be rewarding in diagnosing as well as early case management of anticipated DHF and DSS cases. To estimate the magnitude, seasonal-variation, serological as well as hematological aspects of dengue cases. This was a prospective observational study held in Microbiology and Hematology laboratories of our hospital for duration of one year from July-2019 to June-2020. All the suspected dengue cases were subjected to NS1-antigen, IgM and IgG antibody detection. The samples were also tested for platelet count, total count, haematocrit as well as hemoglobin estimation. All 1,550 dengue suspected cases were subjected to serological testing, among which 157 (10.1%) were positive. The most affected populations were the adult male. As the study was conducted for one year, we could observe the seasonal trend which peaked during post-monsoon. Out of 157 cases, 81.5%, 0.6% and 17.8% were determined as primary, secondary and old dengue cases respectively. There was a significant association between NS1 antigen and fever of </= 5 days duration with ‘p’ value< 0.00001. Thrombocytopenia, leucopenia and increased haematocrit were witnessed in 15.9%, 28.6% and 35% respectively. Our study shows that we had a high magnitude of primary cases that are prone to secondary dengue infection which might have a catastrophic effect giving rise to DHF, DSS or SD.

scholarly journals Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

2011 ◽  
Vol 18 (12) ◽  
pp. 2095-2101 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Mark S. Bailey ◽  
Ampai Tanganuchitcharnchai ◽  
Kemajittra Jenjaroen ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Dengue Infection ◽  
Antibody Test ◽  
Antibody Detection ◽  
Sample Collection ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Tests

ABSTRACTSix assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.

scholarly journals Trend of Dengue Virus Infection with Seasonal Variation at HIMS, Varanasi, Uttar Pradesh, India

2021 ◽  
Author(s):  
Hari Om Trivedi ◽  
Asim Kumar Singh ◽  
Mukesh Kumar Singh
Keyword(s):  
Platelet Count ◽  
Seasonal Variation ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Uttar Pradesh ◽  
Febrile Illness ◽  
Cerebral Hypoperfusion ◽  
Structural Protein ◽  
Igg Antibody ◽  
Ns1 Antigen

Introduction: Dengue is the most common mosquito borne arboviral infection in India, and it has become endemic in India with consistent episodes. Dengue is an acute febrile illness caused by Dengue Virus (DENV-1, DENV-2, DENV-3, DENV-4), an arthropod of family Flaviviridae, transmitted through the bite of female Aedes aegypti mosquitoes. Although, dengue is more prevalent in humans but also it may sometimes be seen in monkeys. The only animals to be affected when bitten by a mosquito, infected with dengue fever are monkeys other animals do not carry or spread dengue. Patient presents with hyperthermia, headache, severe joint and muscle pain, fatigue and skin rashes. Neurological manifestation in sever dengue results from multiorgan dysfunction due to cerebral edema, haemorrhage due to vascular leak, cerebral hypoperfusion, and electrolyte disturbances. Aim: To study serological markers with association of platelet count and trend of DENV infection with seasonal variation. Materials and Methods: The present retrospective study was conducted for a period of two years in Heritage Institute of Medical Sciences (HIMS), Varanasi, Uttar Pradesh, India, from January 2017 to December 2018. Blood samples were collected for 2140 suspected dengue patients. Out of the serologically positive cases, serological confirmation and platelet count was done for dengue infection. Data was presented as percentages. Results: Total 2140 suspected cases were admitted and out of them 199 (9.3%) were found to be seropositive for dengue infection. Non structural protein 1 (NS1) positive cases out of which total seropositive cases were reported to be 127 (63.81%) and 147 (73.86%) cases were positive for NS1 antigen either alone or along with antibody. Out of the total 199 positive cases thrombocytopenia was observed in 126 (63.31%) cases. Positive cases which were under 15 years of age were reported to be 92 (46.23%). Conclusion: The study concludes that NS1 antigen and IgM- IgG antibody consideration in the diagnosis of dengue infection builds the opportunity of early diagnosis so as to keep away the complications significantly.

Incidence and Pattern of Clinical Dengue Cases among Travelers and NonTravelers in a District of Bangladesh

2020 ◽  
Vol 7 (1) ◽  
pp. 3-7
Author(s):  
Syed Abdul Kader ◽  
Sonia Afrin Rob
Keyword(s):  
Endemic Area ◽  
Dengue Infection ◽  
Laboratory Data ◽  
Data Entry ◽  
Cross Sectional Study ◽  
P Value ◽  
College Hospital ◽  
Cross Sectional ◽  
Test Kit ◽  
Ns1 Antigen

Background: Expansion of Dengue fever caused by a mosquito borne arbovirus to new countries and, from urban to rural settings constitutes an important health problem in the world including Bangladesh. Objective: This study was conducted to evaluate spread of clinical Dengue to previously non-endemic Barisal district and get an idea of how recent this spread is by comparing proportions of non-travelers and travelers to an endemic area among the admitted patients. Methodology: The incidence of dengue infection in Barisal division with and without travel history to known endemic area was investigated in the current cross sectional study from a conveniently selected sample of patients admitted to the medicine department of Sher-e-Bangla medical College Hospital form Barisal district from July 15, through August, to September 15, 2019. Clinical and laboratory data were collected by attending doctors and checked by investigators. The primary diagnostic tool was NS1 antigen detected by SD Bioline Dengue NS1 Ag Test Kit. Data entry and analysis was done by SPSS version 24.0 software. Result: A total number of sample size was 212 admitted patents of whom 138(65.1%) were male, 74 (34.9 %) were female,116 (54.7%) of patients traveled to a known endemic zone, 96 (45.3%) did not; 206 (97.2%) were NS1 positive, 4 (1.9%) were IgM positive, 2 (0.9%) were IgM positive and IgG positive; Of travelling 116 patients, 92(79.3%) were male, 24(20.7%) were female, of travelling 96 patients 46(47.9%) were male, 50(52.1%) were female. p-value was <0.001. Conclusion: The study concluded that Dengue is becoming endemic in previously non-endemic zones like greater Barisal, though travelers still holds the major share of disease burden. Male preponderance in traveling to endemic zone was statistically significant. Bangladesh Journal of Infectious Diseases 2020;7(1):3-7

scholarly journals THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Dr. Tanajee Zade ◽  
Dr. K. Srinivas ◽  
Dr. Akshay Berad
Keyword(s):  
Early Diagnosis ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Tertiary Care ◽  
High Morbidity ◽  
Care Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of  General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words:  Dengue, NS1 Antigen, IgM antibody, Platelet

scholarly journals Analysis of Platelet Indices and its correlation with platelet count In Dengue

2020 ◽  
Vol 11 (SPL2) ◽  
pp. 284-289
Author(s):  
Dhanya Menon ◽  
Ganthimathy Sekhar ◽  
Siddharth A R ◽  
Sridevi M
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Large Cell ◽  
Health Concern ◽  
P Value ◽  
Distribution Width ◽  
Platelet Indices ◽  
Ns1 Antigen ◽  
Automated Hematology Analyzer

Dengue is a global arboviral disease of growing public health concern. Several parameters have been used to detect the infection and severity of dengue. Recent evidence suggests that platelet indices can be used to assess the severity and prognosis of dengue infection. This retrospective study aims at assessing the role of the platelet indices – Mean Platelet Volume (MPV), Platelet Distribution Width, (PDW), P-LCR (Platelet Large Cell Ratio) and Plateletcrit (PCT) and its correlation with the platelet count in 50 Dengue NS1 antigen positive cases and to find the sex ratio and the age-wise distribution of the dengue cases. The indices were obtained using an Automated Hematology analyzer and were compared with the platelet count, considering p-value <0.05 as statistically significant. Thrombocytopenia was found in a majority of the cases and PDW and PLCR were found to be inversely proportional to the platelet count. MPV was found to have no correlation with the platelet count. Hence, we conclude that Platelet count, PDW, and P-LCR can be used as predictors of Dengue infection and severity, whereas MPV cannot be used as a predictor of Dengue infection or severity.

scholarly journals Diagnosis of Dengue Virus Infection in Oral Fluid using Immunochromatographic Tests.

2017 ◽  
Vol 11 (1) ◽  
pp. 6-9
Author(s):  
Tahmina Akther ◽  
Biswajit Paul ◽  
Saif Ullah Munshi ◽  
Shahina Tabassum
Keyword(s):  
Diagnostic Accuracy ◽  
Oral Fluid ◽  
Viral Infections ◽  
Dengue Infection ◽  
High Sensitivity ◽  
Epidemiological Survey ◽  
Igg Antibody ◽  
Serological Tests ◽  
Ns1 Antigen ◽  
Sensitivity Specificity

Dengue viral infections are diagnosed by detecting dengue NS1 antigen and dengue specific IgM/IgG antibody by traditional serological tests using patient's blood. Recently saliva is being used employed for diagnosis of different infectious diseases including dengue. In the present study, dengue NS1 antigen and anti-dengue IgM/IgG antibody were detected using rapid immunochromatographic (ICT) kits for diagnosis of dengue and compared with the results of serum ICT results performed on corresponding sera collected from the same individuals. A total of 215 suspected dengue patients were tested and dengue infection was found in 176 (81.9%) sera and 161 (74.9 %) oral fluid samples. Among the 30 dengue NS1 antigen positive sera, 28 were also positive for NS1 in oral fluid indicating high sensitivity (93.3%), specificity (100%), diagnostic accuracy (99.1%), PPV (100%) and NPV (98.9 %) of testing dengue NS1 antigen in oral fluid. Similarly oral fluid assay for anti-dengue IgM showed sensitivity, specificity, diagnostic accuracy, PPV and NPV of 87.3%, 100.0%, 95.8%, 100.0% and 94.1% respectively. Test for anti-dengue IgM/IgG in oral fluid showed sensitivity, specificity, diagnostic accuracy, PPV and NPV of 89.7%, 100.0%, 98.1%, 100.0% and 97.7% respectively. All these parameters for detection of anti-dengue IgG by ICT showed 100%in oral fluid. Thus, results from this study indicates that detection of dengue NS1 antigen or anti-dengue IgM/IgG in oral fluid is an alternative tool for dengue diagnosis. It may benefits dengue diagnosis especially in infants and children since it is easy to collect and require no additional sample processing. It also has the potential to use for epidemiological survey. Bangladesh J Med Microbiol 2017; 11 (1): 6-9

A PROSPECTIVE STUDY ON VARIOUS SEROLOGICAL MARKERS AND PLATELET COUNT IN DENGUE INFECTION IN AND AROUND KANCHEEPURAM.

2021 ◽  
pp. 45-46
Author(s):  
Hamsadwani K P ◽  
Sneka P ◽  
Sangamithra V
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Febrile Illness ◽  
Antibody Detection ◽  
Serological Markers ◽  
Serum Samples ◽  
Platelet Counts ◽  
Acute Viral Infection ◽  
Ns1 Antigen ◽  
A Prospective Study

INTRODUCTION: Dengue is an acute viral infection with potential fatal complications, presenting with non-specic fever that mimics other febrile illness. Specic antibody detection has been the mainstay of diagnosis which is prone for false positive and negative reactions. The newer parameter NS1 appears to be highly specic and reliable for diagnosis. Therefore, we tried to evaluate the association of platelet counts against NS1 and IgM / IgG in dengue infections. OBJECTIVE: To study the association of thrombocytopenia with NS1 antigen and specic antibodies in dengue infection in kancheepuram population. MATERIALS AND METHODS: Serum samples from clinically suspected dengue cases were tested for NS1,IgM,IgG by immunochromatography – based test. Platelet counts were obtained from all positive cases. Test results of dengue-specic parameters and platelet counts were compared. RESULTS: Of the 612 samples tested, 150 were positive for one or more dengue serological markers. Of the 150, 27(18%) were positive for NS1only, 45(30%)were positive for IgM, 54(36%) were positive for IgG only. Of the 150, 39(26%) showed platelet count less than 1 lakh. CONCLUSION: Inclusion of NS1 in the diagnosis of dengue increases the detection and specicity rate. Thus correlation of platelet count, NS1,IgM and ,IgG gives detailed picture of the stage and prognosis of the disease

scholarly journals Correlation of serological markers and thrombocytopenia in early diagnosis of dengue infection

2018 ◽  
Vol 6 (3) ◽  
pp. 812 ◽  
Author(s):  
Suchita Vikas Ingale ◽  
Aditi Jayant Upadhye ◽  
Jayshree Jayant Upadhye
Keyword(s):  
Platelet Count ◽  
Early Diagnosis ◽  
Indian Subcontinent ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Supportive Therapy ◽  
Hemorrhagic Fever ◽  
Control Measures ◽  
Serum Samples ◽  
Ns1 Antigen

Background: Dengue is an acute viral infection with fatal complications. To reduce the disease burden, early and specific diagnosis of dengue hemorrhagic fever or dengue shock syndrome with supportive therapy reduces morbidity and mortality. This study was undertaken to evaluate the efficacy of NS1 antigen and platelet count.Methods: A total of 200 serum samples were collected from clinically suspected cases of dengue fever. Serum samples were tested for NS1, IgM and IgG and correlated with the platelet count.Results: Out of 200 samples screened in our study, 100 (50%) were positive for dengue parameters, 100 (50%) were negative. Out of 100 positive cases, 95 (95%) cases were positive for NS1antigen either alone or in combination with antibodies. 84 (84%) cases were exclusively positive for NS1 antigen only. Primary infection (positive for NS1 Ag, IgM, NS1 + IgM) was seen in 99 (99%) cases and secondary infection (positive for IgG, NS1 + IgG, IgM + IgG, NS1+ IgM+ IgG) was seen in 1 case (1%). Out of 100 positive cases, thrombocytopenia was observed in 60 (60%) of patients while 40 (40%) had platelet count within normal range.Conclusions: Dengue is endemic to Indian subcontinent. Currently no specific antiviral therapy is available. Measuring platelet count and detecting the NS1 antigen plays a vital role in early diagnosis, management and implementing the control measures in community to avoid spread of dengue.

scholarly journals Dengue infection in central India: a 5 years study at a tertiary care hospital

2017 ◽  
Vol 5 (6) ◽  
pp. 2483 ◽  
Author(s):  
Smita T. Deshkar ◽  
Sharmila S. Raut ◽  
Ravindra K. Khadse
Keyword(s):  
Wide Spectrum ◽  
Dengue Infection ◽  
Tertiary Care ◽  
Central India ◽  
Dengue Shock Syndrome ◽  
Viral Disease ◽  
Antibody Detection ◽  
Care Hospital ◽  
Significant Drop ◽  
Clinical Illness

Background: Dengue is one of the most important mosquito borne viral disease with wide spectrum of clinical presentation and often with unpredictable clinical evolution and outcome. Approximately 50 million infections occur annually world-wide, but what’s the real size of the problem in India?  Nobody truly knows...!!  Present study was carried out to determine seropositivity, clinical profile and seasonal variation of dengue infection in central India.Methods: Study was carried out from January 2012 to December 2016. Blood samples were collected from 15,606 patients with dengue like clinical illness and serum was separated. All the samples were subjected to IgM antibody detection by dengue MAC ELISA.Results: Prevalence of dengue in dengue suspected cases was found to be 24.49% (3,822/15,606). Maximum number of positive cases, 1,548 (40.50%) were in the age group of 0-10 years. Males (60.83%) were affected more than females (39.17%). Peak was observed in the months of August, September, October and November. Common presenting features were fever followed by myalgia, arthralgia, headache and bleeding manifestations. Significant drop in platelet count was observed in patients with dengue shock syndrome and dengue haemorrhagic fever.Conclusions: Number of dengue cases in central India are on increase and continued surveillance is essential to determine epidemiological and seasonal trend.

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