scholarly journals Microbial Pattern and Antibiotic Sensitivity Test Among Eye Infection Patients at Bali Mandara Eye Hospital Bali

2021 ◽  
Vol 6 (2) ◽  
pp. 57-60
Author(s):  
Patria Dewi Pande

Abstract Infection disease of the eye usually found in health care facilities and can caused by viral,bacterial, parasite and fungi. Bali Mandara Eye Hospital was special hospital for eye disease in Bali. In this hospital infection disease of the eye dominated by bacterial conjunctivitis, viral conjunctivitis, corneal ulver, and endophtalmitis. This study was a restropective descriptive study using patient who are take eye secret culture in Bali Mandara Eye Hospital and sample were take from 2019 till 2020 It can be seen that was found seven microba and dominated disease was corneal ulcer 26 sample (86%) followed by endophtalmitis 4 sample (14%). The most microba were Pseudomonas aeruginosa. Pseudomonas aeruginosa found resistant in almost antibiotics and sensitive tolevofloxacin, gentamycin dan ceflazidime Keywords: infection, eye, culture, antibiotics

2017 ◽  
Vol 1 (2) ◽  
pp. 23
Author(s):  
Putriana Indah Lestari ◽  
Ika Susanti ◽  
Huda Rahmawati

Abstrak : Penyakit infeksi merupakan salah satu masalah kesehatan yang penting. Penggunaan antibiotik yang tidak rasional dan tepat guna pada pasien penyakit infeksi beresiko menyebabkan terjadinya resistensi. Tujuan dari penelitian ini yaitu untuk mengetahui pola kepekaan bakteri terhadap antibiotik pada pasien Ruang Rawat Intensif (ICU) RSPI Prof. Dr. Sulianti Saroso (RSPI-SS) Jakarta. Penelitian dilakukan deskriptif dan retrospektif terhadap data sekunder hasil uji kepekaan antibiotik dan jenis bakteri dari 107 pasien dalam kurun waktu 2011. Hasil menunjukkan 68 (65,4%) pasien mendapatkan hasil kultur positif dan uji kepekaan bakteri terhadap antibiotik. Jenis bakteri patogen yang dominan yaitu Acinetobacter baumannii (29,4%), disusul oleh Pseudomonas aeruginosa (27,9%), Klebsiella pneumoniae (13,2%) dan Escherichia coli (8,8%). Sebagian besar bakteri pada pasien ICU RSPISS telah berkurang kepekaannya (resisten) terhadap beberapa antibiotik. A. baumannii dan P. aeruginosa merupakan bakteri yang paling resisten terhadap antibiotik uji. Pola kepekaannya menunjukkan bahwa bakteri patogen mempunyai resistensi tertinggi terhadap erythromycin dan terendah terhadap amikasin.Infectious diseases is an important health problem. Irrational antibiotics usage is a leading cause in initiating drugs resistances. A preliminary study was conducted on the sensitivity pattern of microorganisms against antibiotics at the intensive care unit of Sulianti Infectious Diseases Hospital Jakarta. Retrospective. Secondary data were collected on the results of antibiotics sensitivity tests and species of microorganisms of 107 patients during the year 2011. Sixty eight (65,4%) patients were positive on microorganism culture test and tested on antibiotic sensitivity test. Predominance pathogenic species found were Acinetobacter baumannii (29,4%), followed by Pseudomonas aeruginosa (27,9%), Klebsiella pneumoniae (13,2%) and Escherichia coli (8,8%). Most species were less sensitive (resistant) to several antibiotics. The pattern of sensitivity showed that pathogenic microorganisms were the most resistant against erythromycin and the most sensitive antibiotics was amikacin.


2019 ◽  
Vol 43 (2) ◽  
pp. 77-85
Author(s):  
Ikram A.A. Al-Samarraae

This study aimed to isolate Pseudomonas aeruginosa from cattle (bovine) milk with mastitis to characterize its antimicrobial susceptibility against some antibiotics, and to identify aminoglycoside acetyltransferase (aac-3-Ib) gene.A total of 100 bovine milk samples were collected randomly from different local cow farms at districts of Wasit governorate, Iraq. Six P. aeruginosa isolates were obtained using bacterial culture method and further identified by Analytical Profile Index (API-20E). The antibiotic sensitivity test was performed by disc diffusion methods. Among the 5 antibiotics used, the highest resistance (100%) was found with Nalidixic acid andtetracycline, follow by gentamicin (50%) and the lowest resistance rate (16.6%, and 33.3%) was to the ciprofloxacin and cephalothin respectively. PCR was performed for all the gentamicin resistant isolates that be among the aminoglycoside family. The where the frequency of aac(3)-Ib gene have product (530bp) was to 3 of P. aeruginosa isolates. From the finding of present study we concluded that P.aeruginosa thatisolated from mastitic bovinehave been developed resistance against aminoglycosides through presence of aac(3)-Ibgene and the ciprofloxacin and cephalothin can be taken as good choice of treatment


e-CliniC ◽  
2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Monica Lie Sedjati ◽  
Oraetlabora Immanuel Palandeng ◽  
Olivia Claudia Pingkan Pelealu

Abstract: Otitis externa is an inflammation of the ear canal that occur in acute or chronic stage caused by an infection of bacteria, viruses or fungi. The extent of the use of antimicrobials in community can lead to resistant. This study aimed to know the bacteria pattern of otitis externa and the antibiotic sensitivity test at Otorhinolaryngology Departement Prof. Dr. R. D. Kandou Hospital Manado period of November - December 2013. This is a descriptive prospective study. A total of 22 otitis externa patients, the largest age group was adults 15-49 years old with 13 persons (59,1%). Most identified gender was female by 15 people (68.2%). All the patients diagnosed with a history of trauma. The most complained symptoms were pruritus 19 people (86,3%). There were 8 types of bacteria that has been identified and the most identified were 4 samples (18,2%) as Staphylococcus aureus and 4 samples (18,2%) as Pseudomonas aeruginosa. In susceptibility test, the most sensitive antibiotic were Levofloxacin and Ciprofloxacin by 20 samples (100%). The most resistant was Clindamycin 20 sampel (100%). Conclusions : The most identified bacteria were Staphylococcus aureus and Pseudomonas aeruginosa with 4 samples (18.2%). The most sensitive antibiotic were Levofloxacin and Ciprofloxacin 20 samples (100%). Keywords: otitis externa, bacterial pattern, sensitivity test.   Abstrak: Otitis eksterna merupakan peradangan pada liang telinga yang terjadi secara akut maupun kronis yang disebabkan infeksi oleh bakteri, virus maupun jamur. Luasnya penggunaan antimikroba di kalangan masyarakat dapat berujung pada keadaan resisten. Penelitian ini bertujuan untuk mengetahui pola kuman penyebab otitis eksterna dan uji kepekaan antibiotik di poliklinik THT-KL BLU RSUP Prof. Dr. R. D. Kandou Manado periode November-Desember 2013. Penelitian ini bersifat deskriptif prospektif. Dari 22 orang pasien otitis eksterna, diperoleh kelompok umur terbanyak adalah kelompok umur dewasa 15-49 tahun sebanyak 13 orang (59,1%). Jenis kelamin terbanyak adalah perempuan berjumlah 15 orang (68,2%). Semua pasien yang terdiagnosis memiliki riwayat trauma. Gejala yang terbanyak dikeluhkan pasien otitis eksterna adalah pruritus berjumlah 19 orang (86,3%). Terdapat 8 jenis bakteri yang teridentifikasi dan terbanyak adalah Staphylococcus aureus 4 sampel (18,2%) dan Pseudomonas aeruginosa 4 sampel (18,2%). Pada uji kepekaan, antibiotik dengan sensitivitas tertinggi adalah Levofloxacin dan Ciprofloxacin 20 sampel (100%). Angka resistensi tertinggi ditunjukkan oleh Clindamycin 20 sampel (100%). Kesimpulan : Bakteri terbanyak adalah Staphylococcus aureus dan Pseudomonas aeruginosa masing -masing 4 sampel (18,2%). Angka kepekaan tertinggi ditunjukkan oleh Levofloxacin dan Ciprofloxacin 20 sampel (100%). Kata Kunci: otitis eksterna, pola kuman, uji kepekaan.


2017 ◽  
Vol 28 (1) ◽  
pp. 35
Author(s):  
Saba R. Khdair

A total of 50 environmental Pseudomonas aeruginosa isolates were collected from sewage and tap water in Baghdad, Iraq. The MICs of Cefotaxime and Ceftazidime were determined by using agar dilution method, The MIC ranged from 2 to 256 µg/ml.The results of antibiotic sensitivity test showed that among sewage P. aeruginosa isolates, resistance was observed most often to Ticarcillin (92%), Penicillin G (84%), Ceftazidime (12%), (8%) for each of Cefotaxime and Ticarcillin. On the other hand, all tap water isolates were sensitive to Ofloxacin and Levofloxacin, Except (5%) of isolates were resistant to Cefotaxime (25%) to Ceftazidime and (95%) to Ticarcillin. All isolates were tested for Extended-Spectrum β-Lactamase (ESBL) production. Ten isolates (20%) were found to be ESBL producers. All environmental P. aeruginosa isolates were screened for the presence of the blaCTX-M genes by application PCR, Only (30%) of them were positive for this test.


2021 ◽  
Vol 21 (2) ◽  
pp. 64-71
Author(s):  
FAUZAN ARISANDI ◽  
SAFIKA . SAFIKA ◽  
FACHRIYAN HASMI PASARIBU

The use of antibiotics is an alternative to reduce disease incidence and is used to increase egg-laying hens. This study aims to detect the presence of antibiotic resistance to resistance coding genes in Pseudomonas aeruginosa bacteria. The cloacal swab samples were collected randomly as many 66 samples from several egg-laying hens farms in Cianjur Regency, West Java. The collected isolates were examined bacteriologically. The results of the antibiotic sensitivity test of 8 samples (12.1%) of the Pseudomonas aeruginosa showed the resistance level of antibiotics class tetracycline (tetracycline50%, oxytetracycline75%, and doxycycline50%), fluoroquinolone (enrofloxacin 12.5%), and phenicol (chloramphenicol 12.5%). Pseudomonas aeruginosa was resistant to tetracyclines, oxytetracyclines, doxycycline, enrofloxacin and is sensitive to chloramphenicol. The detection of resistance coding genes showed the genes amount of tetA (62.5%) and qnrS (75%). Efforts to determine the level of resistance and use of antibiotics in farms need regular surveillance and monitoring to ensure the wise use of antibiotics.


Author(s):  
Immaculata U. Nwankwo ◽  
Kelechi C. Edward ◽  
Chinedu N. Nwoba ◽  
Chinyere V. Okwudiri

Introduction: The skin is a barrier that limits invasion and growth of pathogenic bacteria. Bacterial species in patients with skin infection and their antibiogram were evaluated for the presence of inhabiting bacteria. Method: The sample obtained with swab sticks were streaked on their respective culture plates containing nutrient agar, MacConkey agar and blood agar, incubated 370 for 24h. The recovered bacteria after incubation were characterized and identified according to standard microbiological criteria. Results: Five bacteria species were isolated which includes Staphylococus aureus; Escherichia coli, Klebsiella specie, Proteus sp and Pseudomonas aeruginosa. Staphylococus aureus (33.03%) had the highest percentage occurrence while Proteus Sp (9.17%) had the lowest percentage occurrence. The highest number of isolates were recovered from male patients (61.47%). Age wise the least percentage of isolates were gotten from age group 31-40 (1.83%) while the highest was obtained from those within the age range of 11-20 (40.37%) followed by 1-10 years of age (25.69%). More isolates were recovered from in-patients (68.81%) than from Out-patients (31.19%). The antibiotic sensitivity test showed that Klebsiella sp, Proteus spp and Staphylococcus aureus were sensitivity to all the antibiotics tested while pseudomonas aeruginosa had 30% activity (sensitivity to only 3 drugs) for the antibiotics used in this study. Conclusion: From this study, it can be said that Staphylococcus aureus, Escherichia coli, Klebsiella sp, Proteus sp and Pseudomonas aeruginosa are implicated in the skin infections in humans and their presence if not treated will lead to long term disease in human affected.


2019 ◽  
Vol 12 (10) ◽  
pp. 1524-1528
Author(s):  
A. N. Dapgh ◽  
A. S. Hakim ◽  
H. A. Abouelhag ◽  
A. M. Abdou ◽  
E. A. Elgabry

Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are able to live everywhere and suggested to be the causative agent of great scope of acute and chronic animal infections. Aim: The current study was carried out to illustrate the prevalence of P. aeruginosa in small ruminants and existence of some virulence operons as well as its antimicrobial resistance. Materials and Methods: A total of 155 samples from sheep and 105 samples from goats (mouth abscesses, fecal swabs, nasal, tracheal swabs, and lung tissue) were collected for bacteriological study, existence of some virulence expression operons with the study of their sensitivity to the antimicrobials using disc diffusion and presence of mexR operon which is responsible for multidrug resistance (MDR). Results: The bacteriological examination revealed that P. aeruginosa was isolated from nine out of 155 samples from sheep (5.8%) and four isolates out of 105 samples from goat (3.8%). It is found that 12 (92.3%), 10 (76.9 %), and 8 (61.5%) of P. aeruginosa isolates harbored hemolysin phospholipase gene (pclH), gene (exoS), and enterotoxin gene (toxA), respectively. The results of antibiotic sensitivity test showed that all tested isolates were resistant to ampicillin, bacitracin, erythromycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, and tobramycin but sensitive to ciprofloxacin and norfloxacin. The MDR (mexR) operon was existed in all isolates. Conclusion: There is a growing risk for isolation of virulent MDR P. aeruginosa from sheep and goat illness cases, and this should be regarded in the efficient control programs.


2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Alke Rumimpunu

Abstract: Otitis Media, an infection of middle ear, is one of the health problems that can cause hearing loss to total deafness. The causes of otitis media are inter alia infections of the aerob bacteria. In Indonesia, the incidence of otitis media is 3.9-6.9 %. Patience compliance due to treatment, germ resistance, anatomy of the ear, and complications lead to difficulties in terms of treatment of the otitis media itself. This study aimed to determine the pattern of bacteria and their susceptibility to antibiotics in patients with otitis media. This was a descriptive prospective study with samples of 20 patients diagnosed with otitis media who met the inclusion criteria. The patients’ ear discharge was used for identifiaction and culture of bacteria in blood agar, nutrient agar and McConkey agar. Gram staining was used for bacteria identificatiion. Levofloxacin and cyprofloxacin were used for antibiotic sensitivity test. Conclusion: The identified bacteria were Staphylococcus aureus, Enterobacter Aerogenes, Staphylococcus Epidermitis, Proteus vulgaris, Cibrobacter divertus, Alcaligenes falcelus, and Pseudomonas aeruginosa. Bacterial growth were mostly found in children. Almost all bacteria were sensitive to both antibiotics, but they were fairly resistant to clindamycin and erythromycin.Keywords: patterns of bacteria, sensitivity test, otitis mediaAbstrak: Otitis media merupakan salah satu gangguan kesehatan telinga yang dapat menyebabkan gangguan pendengaran hingga tuli. Gangguan pada otitis media terletak di telinga bagian tengah. Penyebab otitis media antara lain infeksi bakteri aerob. Di Indonesia, angka kejadian otitis media sebesar 3,9-6,9%. Ketidakpatuhan penderita dalam perawatan, kuman yang resisten, bentuk anatomi telinga, dan adanya komplikasi menyebabkan kesulitan dalam hal pengobatan dan perawatan otitis media. Penelitian ini bertujuan untuk mengetahui pola bakteri dan kepekaannya terhadap antibiotika pada penderita otitis media. Penelitian ini bersifat deskriptif prospektif dengan sampel sebanyak 20 pasien yang didiagnosis otitis media yang memenuhi kriteria inklusi. Sekret telinga diambil untuk identifikasi kuman dan kultur pada agar darah, agar nutrien, dan agar McConkey. Pengecatan Gram dilakukan untuk identifikasi kuman dan uji kepekaan dilakukan terhadap antibiotika levofloxasin dan ciprofloxasin. Simpulan: Bakteri yang diidentifikasi yaitu Staphylococcus aureus, Enterobacter Aerogenes, Staphylococcus Epidermitis, Proteus vulgaris, Cibrobacter divertus, Alcaligenes falcelus, dan Pseudomonas aeruginosa. Pertumbuhan kuman tersering ditemukan pada anak-anak. Hampir semua bakteri peka terhadap antibiotika levofloxacin dan ciprofoxacin tetapi memperlihatkan tingkat resistensi cukup tinggi terhadap clindamycin dan eritromycin. Kata kunci: pola bakteri, uji kepekaan, otitis media


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